• 한국구조물진단유지관리공학회 논문집
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• 제12권1호
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• pp.91-100
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• 2008

다단계 온도프리스트레싱 공법은 기존의 프리스트레싱 공법과 단면접합공법의 장점을 이용한 새로운 개념의 프리스트레싱 공법으로 프리스트레싱용 커버플레이트를 다단계로 가열하여 거더의 하부플랜지에 고장력볼트로 접합한 뒤, 열원을 제거하여 발생하는 다단계 수축력을 보강에 필요한 프리스트레싱력으로 이용한 공법이다. 본 연구는 다단계 온도프리스트레싱 공법의 실제적인 적용성 평가를 위하여 다단계 가열을 위한 가열 장비의 가열성능 평가 실험을 실시하였으며, 라멘형 강거더 임시교량을 제작하고 다단계 온도프리스트레싱 공법을 적용하여 도입 프리스트레싱력을 확인하였다. 또한, 트럭을 이용한 하중재하 실험을 실시하여 구조해석 결과와 비교하였다.

• 한국구조물진단유지관리공학회 논문집
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• 제21권3호
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• pp.76-85
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• 2017

본 연구에서는 유압식 급속재하 시험 장치를 제작하여 변형 속도에 따른 후크형 강섬유 및 폴리아미드 섬유보강 시멘트 복합체의 압축강도 및 인장강도 특성을 평가하였다. 그 결과, 변형 속도가 증가함에 따라 압축강도, 최대 응력 점에서의 변형 및 탄성계수는 증가하였으며, 섬유 종류 및 혼입률은 변형 속도에 의한 압축강도의 영향은 크지 않았다. 본 연구에서 평가된 압축강도의 DIF는 CEB-FIP model code 2010에 비해 상회하였으며, ACI-349의 예측값과 유사한 경향이 나타났다. 인장특성의 경우에도 변형 속도가 증가함에 따라 인장강도와 변형능력이 크게 향상되었다. 후크형 강섬유보강 시멘트 복합체는 변형 속도가 증가함에 따라 섬유와 매트릭스의 부착력이 증가하는 것에 의해 인장강도와 변형능력이 크게 향상되었으며, 섬유가 매트릭스로부터 인발되는 파괴 특성이 나타났다. 한편, 폴리아미드 섬유보강 시멘트 복합체의 경우 섬유와 매트릭스의 부착력이 크기 때문에 섬유가 매트릭스로부터 인발되지 않고 끊어지는 파괴 특성이 나타났으며, 폴리아미드 섬유보강시멘트 복합체의 인장특성에 대한 변형 속도 효과는 섬유의 인장강도에 큰 영향을 받는 것으로 판단되었다. 이러한 결과로부터 폴리아미드 섬유보강 시멘트 복합체의 인장강도에 대한 변형 속도의 효과는 후크형 강섬유의 부착력에 대한 민감도 보다 큰 것으로 사료된다.

• KSBB Journal
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• 제27권4호
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• pp.232-236
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• 2012

Comparative liquid chromatographic enantiomer separation of ${\alpha}$-amino acids, their esters and primary amino compounds was performed using two chiral stationary phases (CSPs) prepared by covalently bonding (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA) of the same chiral selector. In general, the separation factors and resolution factors for these analytes on CSP 1 were greater than on CSP 2, while these capacity factors on CSP 2 were quite greater than on CSP 1. Except for leucine methyl ester and phenylalanine methyl ester, the elution orders of all analytes including ${\alpha}$-amino ${\alpha}$-alkyl acids and phenylglycine alkyl esters on CSP 1 are identical to those on CSP 2. This study showed that different connecting structures for these two CSPs might influence their ability to resolve the analytes depending on their structures related to the chiral recognition mechanism.

• BMB Reports
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• 제45권8호
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• pp.476-481
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• 2012

Flavodoxin (Fld) has been demonstrated to bind to ferredoxin-NADP$^+$ reductase A (FprA) in Pseudomonas putida. Two residues ($Phe^{256}$, $Lys^{259}$) of FprA are likely to be important for interacting with Fld based on homology modeling. Site-directed mutagenesis and pH-dependent enzyme kinetics were performed to further examine the role of these residues. The catalytic efficiencies of FprA-$Ala^{259}$ and FprA-$Asp^{259}$ proteins were two-fold lower than those of the wild-type FprA. Homology modeling also strongly suggested that these two residues are important for electron transfer. Thermodynamic properties such as entropy, enthalpy, and heat capacity changes of FprA-$Ala^{259}$ and FprA-$Asp^{259}$ were examined by isothermal titration calorimetry. We demonstrated, for the first time, that $Phe^{256}$ and $Lys^{259}$ are critical residues for the interaction between FprA and Fld. Van der Waals interactions and hydrogen bonding were also more important than ionic interactions for forming the FprA-Fld complex.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 추계학술대회논문집
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• pp.479-483
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• 2006

As high-definition television(HDTV) broadcasting were generalized, there have been many researches and developments about large storage capacity and fast data transfer rate in optical disk drives(ODD). Pickup actuators must have high flexible mode frequencies and gain margins. Flexible modes are caused by the flexibility of moving parts in the actuator and a servo bandwidth is limited by them. As a result, the system becomes unstable for high-speed operations in high density reading and recording. In this paper, we suggest improved modeling method that considers the bonding layer. And, flexible mode frequency of actuator is improved by Design of Experiment of lens holder. Magnet circuit is designed considering the relation with moving part. Through improving yoke design, the magnetic flux is changed and DC tilt is reduced. Consequently, we designed actuator which has high flexible mode frequency and gain margins.

• BMB Reports
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• 제44권2호
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• pp.118-122
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• 2011

Most widely used secondary structure assignment methods such as DSSP identify structural elements based on N-H and C=O hydrogen bonding patterns from X-ray or NMR-determined coordinates. Secondary structure assignment algorithms using limited $C{\alpha}$ information have been under development as well, but their accuracy is only ~80% compared to DSSP. We have hereby developed SABA (Secondary Structure Assignment Program Based on only Alpha Carbons) with ~90% accuracy. SABA defines a novel geometrical parameter, termed a pseudo center, which is the midpoint of two continuous $C{\alpha}s$. SABA is capable of identifying $\alpha$-helices, $3_{10}$-helices, and $\beta$-strands with high accuracy by using cut-off criteria on distances and dihedral angles between two or more pseudo centers. In addition to assigning secondary structures to $C{\alpha}$-only structures, algorithms using limited $C{\alpha}$ information with high accuracy have the potential to enhance the speed of calculations for high capacity structure comparison.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2003년도 추계학술발표강연 및 논문개요집
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• pp.216-216
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• 2003

The layered nickel oxides (LiNiO$_2$) have been studied for possible use as cathode materials i3l 4V lithium batteries. Although LiCoO$_2$ has been known as the best candidate material for Li-ion batteries, which produces the best performance LiNiO$_2$ is generally accepted as an attractive cathode material, because of its various advantages such as lower cost higher discharge capacity and better reversibility. In this investigation, we calculated the electric state of LiNiO$_2$ using DV-X$\alpha$ molecular orbital method in order to obtain the information of chemical bonding among the Li, Ni and O. In LiNiO$_2$, alternate layers of Li and Ni occupy the octahedral sites of a cubic close packing of oxide ions, making up a rhombohedral structure with an R-3m space group, Li in 3a, Ni in 3b, and O in 6c sites. On the basis of this, we made the cluster model and studied ionization of each atoms and interaction between atoms according to Mullilcen population analysis.

• Computers and Concrete
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• 제8권5호
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• pp.597-616
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• 2011

With respect to rehabilitation, strengthening and retrofitting of existing and deteriorated columns in buildings and bridges, CFRP sheets have been found effective in enhancing the performance of existing RC columns by wrapping and bonding CFRP sheets externally around the concrete. Concrete columns and piers that are confined by both lateral steel reinforcement and CFRP are sometimes referred to as "hybrid" concrete columns. With the availability of experimental data on concrete columns confined by steel reinforcement and/or CFRP, the study presents modeling using artificial neural networks (ANNs) to predict the compressive strength of hybrid circular RC columns. The prediction of the ultimate confined compressive strength of RC columns is very important especially when this value is used in estimating the capacity of structures. The present ANN model used as parameters for the confining materials the lateral steel ratio (${\rho}_s$) and the FRP volumetric ratio (${\rho}_{FRP}$). The model gave good predictions for three types of confined columns: (a) columns confined with steel reinforcement only, (b) CFRP confined columns, and (c) hybrid columns confined by both steel and CFRP. The model may be used for predicting the compressive strength of existing circular RC columns confined with steel only that will be strengthened or retrofitted using CFRP.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.146-154
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• 2020

With the advantages of biocatalytic method, enzymes have been excavated for the synthesis of chiral amino acids by the reductive amination of ketones, offering a promising way of producing pharmaceutical intermediates. In this work, a robust phenylalanine dehydrogenase (PheDH) with wide substrate spectrum and high catalytic efficiency was constructed through rational design and active-site-targeted, site-specific mutagenesis by using the parent enzyme from Bacillus halodurans. Active sites with bonding substrate and amino acid residues surrounding the substrate binding pocket, 49L-50G-51G, 74M,77K, 122G-123T-124D-125M, 275N, 305L and 308V of the PheDH, were identified. Noticeably, the new mutant PheDH (E113D-N276L) showed approximately 6.06-fold increment of k_cat/Km in the oxidative deamination and more than 1.58-fold in the reductive amination compared to that of the wide type. Meanwhile, the PheDHs exhibit high capacity of accepting benzylic and aliphatic ketone substrates. The broad specificity, high catalytic efficiency and selectivity, along with excellent thermal stability, render these broad-spectrum enzymes ideal targets for further development with potential diagnostic reagent and pharmaceutical compounds applications.

• Structural Engineering and Mechanics
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• 제62권5호
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• pp.595-606
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• 2017

During their life span, post-tensioned concrete structures may be exposed to thermal loads. Therefore, there has been a growing interest in research on the advanced analysis and design of post-tensioned concrete slabs subjected to thermal loads. This paper investigates the structural behaviour of post-tensioned one-way spanning concrete slabs. A nonlinear finite element model for the analysis of post- tensioned unbonded and bonded concrete slabs at elevated temperatures was developed. The interface between the tendon and surrounding concrete was also modelled, allowing the tendon to retain its profile shape during the deformation of the slab. The load-deflection behaviour, load-force behaviour in the tendon, and the failure modes are presented. The numerical analysis was conducted by the finite element ANSYS software and was carried out on two different one-way concrete slabs chosen from literature. A parametric study was conducted to investigate the effect of several selected parameters on the overall behavior of post-tensioned one-way concrete slab. These parameters include the effect of tendon bonding, the effect of thermal loading and the effect of tendon profile. Comparison between uniform thermal loading and nonuniform thermal loading showed that restrained post tensioned slab with bottom surface hotter has smaller failure load capacity.