• 한국식품영양과학회지
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• 제21권4호
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• pp.460-470
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• 1992

BAT는 신생아를 비롯하여 동면을 취하는 동물 그리고 설치류에 주로 많은 양이 분포되어 있으며, 세포내지방산을 산화시켜 열을 방출하여 체온조절 및 에너지균형 조절자로서 중요한 역할을 하고 있다. 그러므로 에너지 균형 조절을 통한 체중 조절을 통하여 비만과 밀접한 관련성을 맺고 있는 것으로 보인다. 유전적으로 비만인 실험동물의 경우에는 주로 주위온도, 식이량과 그 구성.성분비 변화와 같은 자극에 대해 민감하게 반응을 일으키지 못해 BAT의 열생성 결함으로 비만이 초래되는 것으로 보인다. 인간의 경우에 있어서는 어느 정도 실험동물의 경우와 비슷한 양상을 보이나, 체내 BAT의 분포량이나 BAT의 연소기질인 지방산의 합성능력 등에 많은 차이점들이 있기 때문에 실험동물의 경우를 인간에게 그대로 적용시킬 수는 없다. 뿐만 아니라 인간을 대상으로 연구하는 데에는 몇가지 문제점들이 있다. 우선 사람의 나이, 성, 실험시작전 영양상태, 스트레스 정도, 유전적 배경 그리고 날씨 에 대한 적응능력에 따라서 열생성 정도가 달라지므로 실험결과에 대한 정확한 해석을 하기가 어렵다. 또한 신생아에 비해서 성인의 경우 BAT의 양이 소량이고 분산되어 있기 때문에 BAT량 결정에 어려움이 있고 열생성 정도와 에너지 소비율과 같이 BAT의 열생성 기준을 나타내는 실험방법에도 많은 문제점이 있는 것으로 나타났다. 그러므로 많은 사람을 대상으로 하여 좀더 정확한 측정방법의 개발을 통해서 열생성에 미치는 타 요인들을 배제 시켜 실험을 하여 소비되는 에너지량을 정확하게 산출하고 BAT의 열생성 기전과 자극원인을 명확히 규명한다면 비만의 치료에 많은 도움을 줄 것으로 생각된다.

• 한국화재소방학회논문지
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• 제30권2호
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• pp.123-132
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• 2016

본 연구는 새로운 차체를 이용 구급자동차 내부 공간의 디자인 작업에 따른 차량 내부의 구조해석을 수행한 결과로서, 해외 기준 및 디자인된 구급자동차를 바탕으로 3D 설계 작업을 수행하였다. 또한, 차체 및 구급자동차 내부에 대하여 10G의 충격을 가한 후 이에 따른 구조해석 결과를 살펴보았다. 이때 부품의 자중과 구성품의 무게를 고려하여 유럽의 EN 규정에 따라 해석을 수행하였다. 구조해석 결과, 차량 내부의 프레임과 각종 파이프로 구성되어진 핸드레일은 비교적 큰 응력의 하중은 받지 않았으나 내장 판넬과 캐비넷류는 50MPa 이상의 큰 응력을 받는 것으로 해석되었다. 이에 따른 보강 설계를 실시하는 경우에는 두께 및 형상의 수정이 필요하다는 결론을 얻을 수 있었다. 이와 같은 연구 결과를 바탕으로 국내 구급자동차를 이용하는 구급대원 및 차량내부의 환자에게 보다 안전한 차량을 제작하는 기초자료가 될 수 있을 것이라 기대된다.

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1132-1141
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• 2020

Inflammatory bowel disease (IBD) is an increasing global burden and a predisposing factor to colorectal cancer. Although a number of treatment options are available, the side effects could be considerable. Studies on fecal microbiota transplantation (FMT) as an IBD intervention protocol require further validation as the underlying mechanisms for its attenuating effects remain unclear. This study aims to demonstrate the ameliorative role of FMT in an ulcerative colitis (UC) model induced by dextran sulfate sodium (DSS) and elucidate its relative mechanisms in a mouse model. It was shown that FMT intervention decreased disease activity index (DAI) levels and increased the body weight, colon weight and colon length of experimental animals. It also alleviated histopathological changes, reduced key cytokine expression and oxidative status in the colon. A down-regulated expression level of genes associated with NF-κB signaling pathway was also observed. The results of 16S rRNA gene sequencing showed that FMT intervention restored the gut microbiota to the pattern of the control group by increasing the relative abundance of Firmicutes and decreasing the abundances of Bacteroidetes and Proteobacteria. The relative abundances of the genera Lactobacillus, Butyricicoccus, Lachnoclostridium, Olsenella and Odoribacter were upregulated but Helicobacter, Bacteroides and Clostridium were reduced after FMT administration. Furthermore, FMT administration elevated the concentrations of SCFAs in the colon. In conclusion, FMT intervention could be suitable for UC control, but further validations via clinical trials are recommended.

• 대한한방소아과학회지
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• 제33권2호
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• pp.12-21
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• 2019

Objectives Coptidis Rhizoma and Glycyrrhiza Uralensis are herbs that treat obesity and dampness-phlegm. The aim of this study is to investigate the efficacy of Coptidis Rhizoma and Glycyrrhiza Uralensis on lipid deposition with nonalcoholic fatty liver disease (NAFLD). Methods Male 6-week-old C57BL/6 male mice were divided into three groups: control group (Ctrl), high fat diet group (HFF), and high fat diet with Coptidis Rhizoma and Glycyrrhiza Uralensis extract administration group (CGT). Each 10 mice were allocated to each group (total of 30 mice). All mice were allowed to eat fat rich diet freely throughout the experiment. To examine the effect of Coptidis Rhizoma and Glycyrrhiza Uralensis, we observed weight changes, total cholesterol and glucose levels, lipid blot distributions, PGC-1, p-$I{\kappa}B$, and p-JNK. Results Body weights for all mice were measured and analyzed the difference between the groups. Weight gain was significantly lower in CGT group than the HFF group. Total cholesterol and glucose levels were significantly lower in CGT group. The distribution of lipid blots and positive reaction of PGC-1 were significantly lower in CGT group. The positive reaction of p-$I{\kappa}B$ in hepatic tissues was significantly lower in CGT group. The positive reaction of p-JNK in hepatic tissues was significantly lower in CGT group. Conclusions Coptidis Rhizoma and Glycyrrhiza Uralensis have the effect of improving non - alcoholic fatty liver induced insulin resistance through regulation of lipid metabolism.

• Animal Bioscience
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• 제34권4호
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• pp.621-632
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• 2021

Objective: This study was conducted to investigate the effects of supplementation with rumen-protected γ-aminobutyric acid (GABA) on carcass characteristics and meat quality of Hanwoo steers. Methods: Eighteen Hanwoo steers with an average initial weight of 644.83±12.91 kg were randomly allocated into three different groups. Each group consisted of 6 animals that were treated with different diets formulated based on the animals' body weights. The control (C) group was fed a basal diet consisting of concentrate and rice straw with 74% total digestible nutrients (TDNs) and 12% crude protein (CP). The two other groups were treatment groups; one group was fed a basal diet (74% TDNs and 12% CP) supplemented with rumen-protected GABA at a dose of 150 mg/kg feed, and the other group was fed a basal diet (74% TDNs and 12% CP) supplemented with GABA at a dose of 300 mg/kg feed. Results: The GABA supplementation significantly contributed to better growth performance (p<0.05), especially the weight gain and average daily gain. It also contributed to the lower cooking loss (p<0.05), improvements in essential antioxidant enzymes and stable regulation of antioxidant activities in the longissimus lumborum of Hanwoo steers, as represented by the lower formation of malondialdehyde content within the meat, the inhibition of myoglobin oxidation indicated by the retention of the oxymyoglobin percentage, and the suppression of metmyoglobin percentage during cold storage (p<0.05). Conclusion: Higher doses of GABA may not significantly promote better animal performance and meat quality, suggesting that dietary supplementation with GABA at a dose of 100 ppm is sufficient to improve the meat quality of Hanwoo steers.

• 산업식품공학
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• 제22권4호
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• pp.358-365
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• 2018

통곡물 시리얼(Whole grain cereal, WGC)이 함유된 식이는 에너지 대사 조절에 중요한 다량영양소(macronutrients)를 제공한다. 본 연구는 고지방식이(high-fat diet, HFD)로 유발된 비만 마우스를 이용하여 WGC의 근감소성 비만 예방 효과에 대해 평가하였다. C57BL/6N 마우스에 정상식이(normal diet, ND), ND+WGC, HFD, HFD+WGC를 12주 동안 제공하였다. WGC는 체중, 식이효율, 체지방 및 지방세포의 크기를 감소시켰다. 또한, WGC는 간 무게 및 간에 축적된 지방을 감소시킴으로써 HFD에 의한 비알코올성 지방간을 개선시켰다. 더욱이, WGC는 비만 마우스 및 정상 마우스의 근육 무게 및 근력을 증가시켰다. 따라서, WGC는 지방 축적을 억제하고 근육량을 증가시키므로 근감소성 비만 예방을 위한 기능성 식품으로 사용될 수 있을 것으로 기대된다.

• 대한중풍순환신경학회지
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• 제15권1호
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• pp.1-12
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• 2014

■ Objectives This study was designed to investigate the effects of Ephedra Herba hexane fraction on lipid levels in serum and lipid accumulation in liver tissue in Hyperlipidemic mice. ■ Methods Hyperlipidemia was induced by providing high fat diet for 4 weeks. Normal group was provided with normal diet. CTL groupwas provided with high fat diet. Ephedra Herba hexane fraction group was provieded with high fat diet and administered orally in the concentration of 1.5mg/kg body weight/day for 2 weeks. In this experiment, effects on total cholesterol, HDL-cholesterol, triglyceride, AST, ALT, fasting blood glucose in serum were measured. In addition histopathological changes in liver tissue were also observed. ■ Results Ephedra Herba hexane fraction did not affects weight gain, serum AST and ALT in hyperlipidemic mice. Oral administration of Ephedra Herba hexane fraction lowered levels of total cholesterol and triglyceride, which were elevated by induction of hyperlipidemia. In addition, Ephedra Herba hexane fraction group showed downward tendency of lipid accumulation compared with CTL group. Finally, administration of Ephedra Herba hexane fraction lowered fasting blood glucose significantly. And Ephedra Herba hexane fraction also ameliorates anti-oxidative stress systems in internal organs which play key role in disease prevention. ■ Conclusion These results suggest that Ephedra Herba hexane fraction can prevent lipid accumulation in liver tissue through regulation of dyslipidemia and hyperglycaemia.

• 대한약침학회지
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• 제16권3호
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• pp.11-22
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• 2013

Objectives: The present investigation aimed at examining if post-cancer treatment with a potentized homeopathic drug, Condurango 30C, which is generally used to treat oesophageal cancer, could also show an ameliorating effect through apoptosis induction on lung cancer induced by benzo[a]pyrene (BaP) in white rats (Rattus norvegicus). Methods: Lung cancer was induced after four months by chronic feeding of BaP to rats through gavage at a dose of 50 mg/kg body weight for one month. After four months, the lung-cancer-bearing rats were treated with Condurango 30C for the next one ($5^{th}$), two ($5^{th}-6^{th}$) and three ($5^{th}-7^{th}$) months, respectively, and were sacrificed at the corresponding time-points. The ameliorating effect, if any, after Condurango 30C treatment for the various periods was evaluated by using protocols such as histology, scanning electron microscopy (SEM), annexinV-FITC/PI assay, flow cytometry of the apoptosis marker, DNA fragmentation, reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and western blot analyses of lung tissue samples. Results: Striking recovery of lung tissue to a near normal status was noticed after post-cancerous drug treatment, as evidenced by SEM and histology, especially after one and two months of drug treatment. Data from the annexinV-FITC/PI and DNA fragmentation assays revealed that Condurango 30C could induce apoptosis in cancer cells after post-cancer treatment. A critical analysis of signalling cascade, evidenced through a RT-PCR study, demonstrated up-regulation and down-regulation of different pro- and anti-apoptotic genes, respectively, related to a caspase-3-mediated apoptotic pathway, which was especially discernible after one-month and two-month drug treatments. Correspondingly, Western blot and immunohistochemistry studies confirmed the ameliorative potential of Condurango 30C by its ability to down-regulate the elevated epidermal growth factor receptor (EGFR) expression, a hallmark of lung cancer. Conclusion: The overall result validated a positive effect of Condurango 30C in ameliorating lung cancer through caspase-3-mediated apoptosis induction and EGFR down-regulation.

• 대한본초학회지
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• 제30권4호
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• pp.37-44
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• 2015

Objectives : The present study was designed to evaluate the anti-inflammatory and anti-oxidative stress activities through regulation of Nrf2-mediated genes by Rhei rhizoma and Glycyrrhiza rhizoma combined extract (RGE) in reflux esophagitis.Methods : The antioxidant activity of RGE in vitro was measured in terms of radical scavenging capacity such as DPPH and ABTS. RGE was administered at 350 mg/kg body weight prior to induction of reflux esophagitis. Reflux esophagitis was induced that tied the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats.Results : RGE scavenged DPPH and ABTS effectively and IC₅₀of RGE each were 4.9 μg/ml and 45.6 μg/ml. Our results show that RGE administration markedly ameliorated mucosal damage upon histological evaluation. In serum and esophagus tissue, RGE significantly suppressed the oxidative stress biomarkers. Reflux esophagitis induced rats exhibited down-regulation of antioxidant-related proteins in the esophagus; however, the levels with treatment of RGE were significantly higher than those of vehicle reflux esophagitis rats. RGE treatment caused significant reductions in activation of NF-κB transcription factor. Thus, RGE significantly exhibited potent anti-inflammatory activities by suppressing the protein expression levels of pro-inflammatory proteins such as COX-2 and iNOS and inflammatory cytokines such as TNF-αin the esophagus tissue.Conclusions : Reflux esophagitis caused considerable levels of oxidative stress in the esophageal mucosa and the administration of RGE reduced the esophageal mucosa damage through the regulation of Nrf2 and NF-κB pathways. Our findings can considered as supplementary therapy in the prevention or treatment of reflux esophagitis.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4597-4606
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• 2012

Cholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells of the bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activity of a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activity against a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was performed by DNA fragmentation assay and induction of genes that may be involved in the resistance of CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. To investigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/DMN)-induced CCA hamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily or every alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with 5-fluorouracil (positive control) or distilled water (untreated control). Median $IC_{50}$ (concentration that inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolic extract of ginger were 10.95, 53.15, and $27.86{\mu}g/ml$, respectively. More than ten DNA fragments were visualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed following exposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of any significant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage. The survival time and survival rate of the CCA-bearing hamsters were significantly prolonged compared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivo studies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCA with the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved in conferring resistance of CCA to the ginger extract.