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http://dx.doi.org/10.6116/kjh.2015.30.4.37.

Improving Effect of a Combined Extract of Rhei Rhizoma and Glycyrrhizae Rhizoma through Anti-oxidative Stress in Reflux Esophagitis rats  

Kim, MinYeong (College of Korean Medicine, Daegu Hanny university)
Shin, YuOck (College of Korean Medicine, Daegu Hanny university)
Lee, JooYoung (College of Korean Medicine, Daegu Hanny university)
Lee, AhReum (College of Korean Medicine, Daegu Hanny university)
Shin, SungHo (College of Korean Medicine, Daegu Hanny university)
Kwon, OJun (Gyeongbuk Regional industry Evalution, Daegyeong Institute for Regional Program Evalution)
Seo, BuIl (College of Korean Medicine, Daegu Hanny university)
Roh, Seong-Soo (College of Korean Medicine, Daegu Hanny university)
Publication Information
The Korea Journal of Herbology / v.30, no.4, 2015 , pp. 37-44 More about this Journal
Abstract
Objectives : The present study was designed to evaluate the anti-inflammatory and anti-oxidative stress activities through regulation of Nrf2-mediated genes by Rhei rhizoma and Glycyrrhiza rhizoma combined extract (RGE) in reflux esophagitis.Methods : The antioxidant activity of RGE in vitro was measured in terms of radical scavenging capacity such as DPPH and ABTS. RGE was administered at 350 mg/kg body weight prior to induction of reflux esophagitis. Reflux esophagitis was induced that tied the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats.Results : RGE scavenged DPPH and ABTS effectively and IC50of RGE each were 4.9 μg/ml and 45.6 μg/ml. Our results show that RGE administration markedly ameliorated mucosal damage upon histological evaluation. In serum and esophagus tissue, RGE significantly suppressed the oxidative stress biomarkers. Reflux esophagitis induced rats exhibited down-regulation of antioxidant-related proteins in the esophagus; however, the levels with treatment of RGE were significantly higher than those of vehicle reflux esophagitis rats. RGE treatment caused significant reductions in activation of NF-κB transcription factor. Thus, RGE significantly exhibited potent anti-inflammatory activities by suppressing the protein expression levels of pro-inflammatory proteins such as COX-2 and iNOS and inflammatory cytokines such as TNF-αin the esophagus tissue.Conclusions : Reflux esophagitis caused considerable levels of oxidative stress in the esophageal mucosa and the administration of RGE reduced the esophageal mucosa damage through the regulation of Nrf2 and NF-κB pathways. Our findings can considered as supplementary therapy in the prevention or treatment of reflux esophagitis.
Keywords
Rhei Rhizoma; Glycyrrhiza Rhizoma; gastroesophagea reflux disease; oxidative stress; anti-inflammation; antioxidation;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 National Health Insurance Service. 『Stmach-reflux esophagitis disease』, improve by the correct eating habits. Retrieved May. 05, 2015, from http://www.nhis.or.kr/bbs7/boards/B0039/13739.
2 Badillo R, Francis D. Diagnosis and treatment of gastroesophageal reflux disease. World J Gastrointest Pharmacol Ther. 2014 ; 5(3) : 105-12.   DOI
3 Lee JS, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Kim HJ, Hahm KB. Involvement of oxidative stress in experimentally induced reflux esophagitis and Barrett's esophagus: clue for the chemoprevention of esophageal carcinoma by antioxidants. Mutat Res. 2001 ; 480-481 : 189-200.   DOI   ScienceOn
4 Oh TY, Lee JS, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Cho SW, Lee KM, Hahm KB. Oxidative stress is more important than acid in the pathogenesis of reflux oesophagitis in rats. Gut. 2001 ; 49(3) : 364-71.   DOI
5 Kim BH. Effects of Rhei Rhizoma on Gastric Ulcer in Sprague-Dawley Rats. Korean J Orient Physiol Pathol. 2011 ; 5(1) : 71-7.
6 Lee SN, Kim HK, Seo BL. A philological study on poisoning of Rhei Radix et Rhizoma(大黃). J East-West Med. 2012 ; 37(3) : 13-24.
7 The whole country a college of Oriental medicine. The joint textbook publish commission compilation. Herbology. Seoul : Younglimsa. 1995 : 242-3.
8 He ZH, He MF, Ma SC, But PP. Anti-angiogenic effects of rhubarb and its anthraquinone derivatives. J Ethnopharmacol. 2009 ; 121(2) : 313-7.   DOI   ScienceOn
9 Huang Q, Lu G, Shen HM, Chung MC, Ong CN. Anti-cancer properties of anthraquinones from rhubarb. Med Res Rev. 2007 ; 27(5) : 609-30.   DOI   ScienceOn
10 Jeon SG, Rhee PL. Prevalence, Clinical Manifestations, and Treatment of Gastroesophageal Reflux Disease in Korea. Korean J Gastroenterol. 2008 ; 51(6) : 331-7.
11 Kim J, Cha YN, Surh YJ. A protective role of nuclear factor-erythroid 2-related factor-2 (Nrf2) in inflammatory disorders. Mutat Res. 2010 ; 690(1-2) : 12-23.   DOI   ScienceOn
12 Lee S, Shin S, Kim H, Han S, Kim K, Kwon J, Kwak JH, Lee CK, Ha NJ, Yim D, Kim K. Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways. J Inflamm (Lond). 2011 ; 8(1) : 16.   DOI   ScienceOn
13 Gi Y. Effects on Squalene about secretion of TNF-ɑ & IL-6 by Glycerol-induced acute-renal failure in mice kidney. Chosun university. 2006.
14 Ekmekcioglu S, Ellerhorst J, Smid CM, Prieto VG, Munsell M, Buzaid AC, Grimm EA. Inducible nitric oxide synthase and nitrotyrosine in human metastatic melanoma tumors correlate with poor survival. Clin Cancer Res. 2000 ; 6(12) : 4768-75.
15 Vincent HK, Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes (Lond). 2006 ; 30(3) : 400-18.   DOI
16 Yoshida N. Inflammation and oxidative stress in gastroesophageal reflux disease. J Clin Biochem Nutr. 2007 ; 40(1) : 13-23.   DOI   ScienceOn
17 Dreger H, Westphal K, Weller A, Baumann G, Stangl V, Meiners S, Stangl K. Nrf2-dependent upregulation of antioxidative enzymes: a novel pathway for proteasome inhibitor-mediated cardioprotection. Cardiovasc Res. 2009 ; 83(2) : 354-61.   DOI   ScienceOn
18 Qiu L, Song Z, Setaluri V. Oxidative stress and vitiligo: the Nrf2-ARE signaling connection. J Invest Dermatol. 2014 ; 134(8) : 2074-6.   DOI   ScienceOn
19 Lee JS, Oh TY, Ahn BO, Cho H, Kim WB, Kim YB, Surh YJ, Kim HJ, Hahm KB. Involvement of oxidative stress in experimentally induced reflux esophagitis and Barrett's esophagus: clue for the chemoprevention of esophageal carcinoma by antioxidants. Mutat Res. 2001 ; 480-481 : 189-200.   DOI   ScienceOn
20 Baeuerle PA, Henkel T. Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994 ; 12 : 141-79.   DOI   ScienceOn
21 Van Antwerp DJ, Martin SJ, Verma IM, Green DR. Inhibition of TNF-induced apoptosis by NF-kappa B. Trends Cell Biol. 1998 ; 8(3) : 107-11.   DOI   ScienceOn
22 O’Neill LA, Kaltschmidt C. NF-kappa B: a crucial transcription factor for glial and neuronal cell function. Trends Neurosci. 1997 ; 20(6) : 252-8.   DOI   ScienceOn
23 Altomare A, Guarino MP, Cocca S, Emerenziani S, Cicala M. Gastroesophageal reflux disease: update on inflammation and symptom perception. World J Gastroenterol. 2013 ; 19(39) : 6523-8.   DOI   ScienceOn
24 Beg AA, Finco TS, Nantermet PV, Baldwin AS Jr. Tumor necrosis factor and interleukin-1 lead to phosphorylation and loss of I kappa B alpha: a mechanism for NF-kappa B activation. Mol Cell Biol. 1993 ; 13(6) : 3301-10.   DOI
25 Kim DJ, Roh SS. Effect on Acute reflux Esophagitis by Evodiae Fructus Aquous Extract. Kor J Herbol. 2012；27(1)：51-8.   DOI   ScienceOn
26 Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med. 1994 ; 16(2) : 149-56.   DOI   ScienceOn
27 Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978 ; 86(1) : 271-8   DOI   ScienceOn
28 El-Serag HB, Sweet S, Winchester CC, Dent J. Update on the epidemiology of gastro-oesophageal reflux disease: a systematic review. Gut. 2014 ; 63(6) : 871-80.   DOI   ScienceOn
29 Wung BS, Hsu MC, Wu CC, Hsieh CW. Piceatannol upregulates endothelial heme oxygenase-1 expression via novel protein kinase C and tyrosine kinase pathways. Pharmacol Res. 2006 ; 53(2) : 113-22.   DOI   ScienceOn
30 Lee HH, Park SA, Almazari I, Kim EH, Na HK, Surh YJ. Piceatannol induces heme oxygenase-1 expression in human mammary epithelial cells through activation of ARE-driven Nrf2 signaling. Arch Biochem Biophys. 2010 ; 501(1) : 142-50.   DOI   ScienceOn
31 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999 ; 26(9-10) : 1231-7.   DOI   ScienceOn
32 Yokozawa T, Chen CP, Tanaka T. Direct scavenging of nitric oxide by traditional crude drugs. Phytomedicine. 2000 ; 6(6) : 453-63.   DOI   ScienceOn
33 Dudonné S, Vitrac X, Coutière P, Woillez M, Mérillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem. 2009 ; 57(5) : 1768-74.   DOI   ScienceOn
34 Hong JS, Kang BG, Jang YS, Kim SH, Wang Z, Park YH, Park JH and Lim SS. Studies on Standardization of Licorice Based on Its Active Components with On-line HPLC Bioassay System. Korean J Plant Res. 2014 ; 27(5) : 401-14.   DOI   ScienceOn
35 Myung SH, Kim YS. Anti-Oxidative and Neuroprotective Effects of Rhei Rhizoma on BV-2 Microglia Cells and Hippocampal Neurons. Korean J Orient Physiol Pathol. 2005 ; 19(3) : 647-55.
36 Son YJ, Kim YS, Lee YJ. The Effects of Rhei palmati Radix and Rhei undulati Radix on the Blood Lipids and Enzymes of Hypercholesterolic Rats. Kor J Herbol. 1999 ; 14(1) : 61-8.
37 Mae T, Kishida H, Nishiyama T, Tsukagawa M, Konishi E, Kuroda M, Mimaki Y, Sashida Y, Takahashi K, Kawada T, Nakagawa K, Kitahara M. A licorice ethanolic extract with peroxisome proliferator-activated receptor-gamma ligand-binding activity affects diabetes in KK-Ay mice, abdominal obesity in diet-induced obese C57BL mice and hypertension in spontaneously hypertensive rats. J Nutr. 2003 ; 133(11) : 3369-77.
38 Park BH. Pharmacological and Physiologicol activities of Glycyrrhizae Radix. Ewha Womans University. 1983.
39 Lee KH, Rhee KH. Anti-Inflammatory Effects of Glycyrrhiza glabra Linne Extract in a Dextran Sulfate Sodium-Induced Colitis Mouse Model. Korean J Food Nutr. 2010 ; 23(4) : 435-9.
40 Jung HY. Pharmacological treatment for reflux esophagitis. J Korean Med Assoc. 2011 ; 54(1) : 88-91.   DOI   ScienceOn
41 Woo KS, Hwang IG, Noh YH, Jeong HS. Antioxidant Activity of Heated Licorice (Glycyrrhiza uralensis Fisch) Extracts in Korea J Korean Soc Food Sci Nutr. 2007 ; 36(6) : 689-95.   DOI   ScienceOn
42 Omura N, Kashiwagi H, Chen G, Suzuki Y, Yano F, Aoki T. Establishment of surgically induced chronic acid reflux esophagitis in rats. Scand J Gastroenterol. 1999 ; 34(10) : 948-53.   DOI