• International Journal of Industrial Entomology and Biomaterials
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• 제21권2호
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• pp.157-162
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• 2010

Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the $(His)_6$-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified $(His)_6$-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.

• 한국정보통신학회논문지
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• 제4권1호
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• pp.219-226
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• 2000

본 논문에서는 WLL 광대역 코드분할 다원 접속(Wideband CDMA) 시스템 기지국용 If(Intermediate Frequency) 송수신기의 설계 및 구현에 대하여 고찰하였다. 제작된 IF 송수신기는 송신단, 수신단, 국부발진기로 구성되었다. 처리되는 신호의 대역폭을 10MHz로 고려하여 IF 반송파는 40MHz로 설계하였으며, 측정 결과 IF 송신단의 출력 전력은 기저 대역 입력이 -10dBm $\pm3dB$ 일 때 40MHz에서 -5dBm $\pm3dB$, 수신단의 출력 전력은 IF 대역 입력이 -5dBm $\pm3dB$ 일 때 기저대역에서 -10dBm $\pm3dB$의 특성을 얻었다. 또한, 자동이득 조절 루프는 -7dBm에서 +2dBm까지의 9dB 입력 범위에서 동작하여 약 2dBm의 일정한 레벨을 출력시켰고, 1MHz부터 5MHz까지의 신호를 스윕시켜 IF시스템 내에서의 위상 변화를 관찰한 결과 위상 왜곡이 매우 적어 데이터 통신시스템에 적용이 가능함을 보였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.25-29
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• 2001

We have cloned and characterized an antiapoptotic gene, p35, which blocks apoptosis, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 897 bp p35 has an open reading frame of 299 amino acids. The BmNPV-K1 p35 showed a high identity to Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain. The BmNPV-K1 p35 was different from the amino acid sequences of BmNPV T3 at 6 positions. The p35 gene of BmNPV-K1 was 99.2% identical at the nucleotide level and 98% identical at the amino acid level to BmNPV T3. The location of p35 gene in the BmNPV-K1 genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were con- firmed by Northern hybridization analysis.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.37-41
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• 2001

The ecdysteroid UDP-glucosyltransferase (egt) gene isolated from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain was compared to its homologue from Autographa californica NPV (AcNPV) and Bm NPV T3. The egt gene of BmNPV-K1 encoded 506 amino acid open reading frame, and was 99.6% identical at the amino acid level and 99.2% identical at the nucleotide level to BmNPV T3. The BmNPV-K1 egt gene showed highly identity to AcNPV and BmNPV T3 strain. The BmNPV-K1 egt gene was different from amino acid sequence at 2 positions, 19 and 72, in BmNPV T3. The genomic location of egt gene in the BmNPV-K1 was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were confirmed by Northern hybridization analysis. Transcripts of the egt of Bm NPV-K1 peaked around 12 hrs postinfection (p.i.) and reduced at 24 hrs p.i.

• 한국통신학회논문지
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• 제33권1A호
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• pp.85-90
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• 2008

Emitter degeneration에 의한 band SiGe HBT 이중 평형형 상향 주파수 혼합기의 선형성 개선 효과를 비교하였다. 시뮬레이션을 통해 출력 전력과 변환 이득을 동시에 고려하여 degeneration 저항값을 최적화 시켰으며 이를 $0.35{\mu}m$ Si-BiCMOS 공정을 이용하여 제작하였다. 제작 및 측정 결과, -5 dBm의 8.0 GHz LO 신호 및 100 MHz의 IF 신호 입력 시, degeneration 저항이 없는 상향 주파수 혼합기는 15.5 dB의 선형 변환 이득과 -13 dBm의 RF 출력 전력 및 3.7 dBm의 $OIP_3$를 나타내었고, degeneration 저항을 사용한 상향 주파수 혼합기는 9 dB의 선형 변환 이득과 -10 dBm의 RF 출력 전력 및 8.7 dBm의 $OIP_3$를 각각 나타내었다.

• 한국잠사곤충학회지
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• 제33권1호
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• pp.21-26
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• 1991

Bm 배양세포주(BmN4)에서 BmNPV의 다각체 단백질 합성과 DNA복제에 대한 실험결과는 다음과 같다. 1. BmNPV는 insect Grace's medium에서 배양되고 있는 BmN4 세포에서 NOV나 DNA로 감염시킨 경우 모두 잘 증식되었으며, 도립현미경 관찰 결과 접종 후 48시간이 경과하면서 다각체가 형성되기 시작하였다. 2. 또한 전자현미경으로 핵내 형성된 다각체와 협성중인 nucleocapsid 다발을 관찰하였으며, 바이러스 입자는 SNPV로 존재하였다. 3. Western blot분속에 의한 다각체 단백질의 합성은 접종 후 18시간부터 관찰되었으며, 다각체 단백질의 분자량은 30kd이었다. 4. Wild type BmNPV DNA와 Bm 세포(BmN4)에서 NOV 및 DNA 감염에 의해 형성된 바이러스 DNA간의 제한효소 패턴은 차이가 없었다.

• Nuclear Engineering and Technology
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• 제54권9호
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• pp.3403-3414
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• 2022

The purpose of this study is to model and optimize the block-matching and 3D filtering (BM3D) algorithm and to evaluate its applicability in brain single-photon emission computed tomography (SPECT) images using a fan beam collimator. For quantitative evaluation of the noise level, the coefficient of variation (COV) and contrast-to-noise ratio (CNR) were used, and finally, a no-reference-based evaluation parameter was used for optimization of the BM3D algorithm in the brain SPECT images. As a result, optimized results were derived when the sigma values of the BM3D algorithm were 0.15, 0.2, and 0.25 in brain SPECT images acquired for 5, 10, and 15 s, respectively. In addition, when the sigma value of the optimized BM3D algorithm was applied, superior results were obtained compared with conventional filtering methods. In particular, we confirmed that the COV and CNR of the images obtained using the BM3D algorithm were improved by 2.40 and 2.33 times, respectively, compared with the original image. In conclusion, the usefulness of the optimized BM3D algorithm in brain SPECT images using a fan beam collimator has been proven, and based on the results, it is expected that its application in various nuclear medicine examinations will be possible.

• 한국정보통신학회논문지
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• 제3권3호
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• pp.517-531
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• 1999

본 논문에서는 INMARSAT-M형 송신기에 사용되는 L-BAND(1626.5-1646.5 MHz)용 2단 가변이득 전력 증폭기를 연구 개발하였다. 2단 가변이득 전력증폭기는 구동증폭단과 전력증폭단에 의해 고출력 모드일 때 +42 dBm, 저출력 모드일 때는 +36 dBm의 전력으로 증폭되며, 각각에 대해 상한 +1 dBm과 하한 -2 dBm의 오차를 허용한다. 제작의 간편성 때문에 전체 2단 가변이득 전력증폭기를 크게 구동증폭단과 전력증폭단 두 부분으로 나누어 구현하였으며, 전력증폭부를 구동하기 위한 구동단은 HP사의 MGA-64135와 Motorola사의 MRF-6401을 사용하였으며, 전력증폭단은 ERICSSON사의 PTE-10114와 PTF-10021을 사용하여 RF부, 온도보상회로 및 출력 조절회로를 함께 집적화 하였다. 이득조절은 구동증폭단의 MGA-64135의 바이어스 전압을 조절하는 방법을 제시하였으며, 실험 결과와 잘 일치하였다. 제작된 2단 가변이득 전력증폭기는 20 MHz대역폭 내에서 소신호 이득이 42 dB와 36 dB 이상, 입ㆍ출력 정재파비는 1.5:1 이하, 5 dBm의 $P_{1dB}$. $P_{ldB}$출력레벨에서 3 dB Back off 시켰을 때 32.5 dBc의 I $M_3$를 얻었다. 1636.5 MHz 주파수에 대해 출력전력은 43 dBm과 37 dBm으로서 설계시 목표로 했던 최대 출력전력 20 Watt를 얻었다.다.다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -2
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• pp.1046-1049
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• 2002

In this paper, a sub-harmonic dual-gate FET mixer for IMT-2000 base-station was designed by using single-gate FET cascode structure and driven by the second order harmonic component of LO signal. The dual-gate FET mixer has the characteristic of high conversion gain and good isolation between ports. Sub-harmonic mixing is frequently used to extend RF bandwidth for fixed LO frequency or to make LO frequency lower. Furthermore, the LO-to-RF isolation characteristic of a sub-harmonic mixer is better than that of a fundamental mixer because the frequency separation between the RE and LO frequency is large. As RF power is -30dBm and LO power is 0dBm, the designed mixer shows the -47.17dBm LO-to-RF leakage power level, 10dB conversion gain, -0.5dBm OIP3, -10.5dBm IIP3 and -1dBm 1dB gain compression point.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.217-223
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• 2004

A cathepsin L-like cysteine protease, v-cath, encoded by the baculovirus has been shown to playa role in host liquefaction. We have identified a v-cath gene in the silkworm virus, Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 969 bp v-cath has an open reading frame of 323 amino acids. A putative cleavage site and catalytic sites were conserved in BmNPV-K1 v-cath. The predicted three-dimensional structure of BmNPV-K1 v-cath revealed that the overall fold of BmNPV-K1 v-cath is similar to that of other proteases of the papain family. The deduced amino acid sequence of BmNPV-K1 v-cath showed 98% and 97% protein sequence identity to BmNPV T3 strain and to Autographa californica nuclear polyhedrosis virus, respectively. The BmNPV-K1 v-cath differed at 4 amino acid positions from BmNPV T3. The v-cath gene in BmNPV-K1 genome is located on the EcoRV 6 kb and XhoI 9 kb fragments. Northern hybridization analysis of BmNPV K1 v-cath gene revealed that it is expressed late in infection.