• Title/Summary/Keyword: Bm60

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Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus

  • Du, Meng-Fang;Yin, Xin-Ming;Guo, Zhong-Jian;Zhu, Liang-Jun
    • BMB Reports
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    • v.39 no.6
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    • pp.737-742
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    • 2006
  • Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight of 31.0 kDa. Bm60 and its homologues have been identified in 11 completely sequenced lepidopteran NPVs. The transcript of Bm60 was detected by RT-PCR at 18-72 h post-infection (p.i.), while the corresponding protein could be detected at 24-72 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm60. The expression of Bm60 was inhibited in the presence of Ara-c, an inhibitor of viral DNA synthesis. These results together indicated that Bm60 was a late gene. The size of Bm60 product was found to be a 31 kDa in BmNPV-infected BmN cells, consistent with predicted molecular weight. Immuno-fluoresence analysis showed that the Bm60 product was first detected in the cytoplasm at 24 h p.i and also located in nucleus during later infection. In conclusion, the available data suggest that Bm60 is a functional ORF of BmNPV and encodes a 31kDa protein expressed in the later stage of infection cycle.

Use of FT-IR to Identify Enhanced Biomass Production and Biochemical Pool Shifts in the Marine Microalgae, Chlorella ovalis, Cultured in Media Composed of Different Ratios of Deep Seawater and Fermented Animal Wastewater

  • Kim, Mi-Kyung;Jeune, Kyung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.19 no.10
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    • pp.1206-1212
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    • 2009
  • Growth rates, photosystem II photosynthesis, and the levels of chlorophyll $\alpha$ and secondary metabolites of Chlorella ovalis were estimated to determine if they were enhanced by the addition of swine urine (BM) or cow compost water (EP) that had been fermented by soil bacteria to deep seawater (DSW) in an attempt to develop media that enabled batch mass culture at lower costs. Growth of C. ovalis in f/2, f/2-EDTA+BM60%, DSW+BM30%, and DSW+EP60% was enhanced and maintained in the log phase of growth for 16 days. The cell densities of C. ovalis in DSW+EP60% ($4.1{\times}10^6$ Cells/ml) were higher than those of f/2 ($2.9{\times}10^6$ Cells/ml), f/2-E+BM60% ($3.7{\times}10^6$ Cells/ml), and DSW+BM30% ($2.7{\times}10^6$ Cells/ml). The growth rate was also more favorable for C. ovalis cultured in DSW+EP60% ($0.15\;day^{-1}$) than that of C. ovalis cultured in the control medium (f/2) ($0.12\;day^{-1}$). Furthermore, the chlorophyll a concentration of C. ovalis cultured in DSW+EP60% (4.56 mg/l) was more than 2-fold greater than that of C. ovalis cultured in f/2 (2.35 mg/l). Moreover, the maximal quantum yields of photo system II at 470 nm (Fv/Fm) were significantly higher in organisms cultured at f/2-E+BM60% (0.53) and DSW+EP60% (0.52) than in the other treatment groups. Finally, Fourier transformation infrared (FT-IR) spectroscopy revealed that C. ovalis grown in DSW+EP60% had more typical peaks and various biochemical pool shifts than those grown in other types of media. Taken together, the results of this study indicate that the use of DSW+EP60% to culture C. ovalis can reduce maintenance expenses and promote higher yields.

Design of Temperature Compensation Circuit for Satisfying the Intermodulation Specification of Power Amplifier (전력증폭기의 혼변조 규격 만족을 위한 온도보상회로 설계)

  • Park, Won-Woo;Kim, Byung-Chul;Cho, Kyung-Rae;Lee, Jae-Buom
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.12
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    • pp.2609-2614
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    • 2010
  • Temperature compensation circuit is implemented by using the temperature sensor, and Intermodulation (IM) Specification of Power Amplifier is satisfied in the temperature range of $-30^{\circ}C{\sim}60^{\circ}C$ with this temperature compensation circuit. The output voltage of temperature compensation circuit which vary 170mV with the temperature is applied to the gate of TR in 3W output power Amplifier. As the result, right 3rd IM component is -18.5~-26dBm, left 3rd IM component is -18.5~-35dBm, and the left and right 5th IM component is -24~-26dBm in the temperature range of $-30^{\circ}C{\sim}60^{\circ}C$. It is confirmed that IM specification of power amplifier which is under -17dBm in the whole temperature range is satisfied.

Design and Implementation an RF-state for WLL multichannel personal stations (WLL 다중채널 단말기용 RF단 설계 및 제작)

  • 장홍주
    • Journal of Korea Society of Industrial Information Systems
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    • v.6 no.2
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    • pp.31-37
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    • 2001
  • In this paper, We designed and implemented an RF module for WLL multi-channel personal stations. Then, we experimentally evaluated in receiving sensitivity, adjacent channel interference selectivity and spurious response limit. From the measurement results on the RF module for WLL multi-channel personal stations, we observed and confirmed as follows: First, the variation in output power level was kept to be less than 30 dBm in the range of the input power level from -90 dBm to -30dBm. This measurement result confirmed the dynamic range of 60 dB. Secondly, at the output stage of the RF module, the gain flatness in the pass band was measured to be less than 2dB, which confirmed a precise control of the bandspread signals. Thirdly, at the RF module, the bandwidth and power level in transmission were measured to be 10 MHz, respectively we note that these features satisfy the design specifications.

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60GHz band RF transceiver of the broadband point-to-point communication system (광대역 점대점 통신시스템용의 60GHz 대역 무선 송수신기)

  • Choi, Jae-Ha;Yoo, Young-Geun
    • Journal of the Institute of Convergence Signal Processing
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    • v.13 no.1
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    • pp.39-43
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    • 2012
  • 60GHz band RF transceiver was made with the NRD waveguide structure for the point- to-point communication. A dielectric line that of comprising NRD waveguide was the milling process was not easy because a material gets soft, and also compression and expansion according to a temperature were serious, so this line was not suitable for the device in which the resonance characteristic was important. In addition, the thing for comprising amplification module was difficult in the NRD waveguide structure. In this paper, a way in which to overcome mentioned in upper part, the transceiver was made by below technology. Components in which the resonance characteristic was not important were made with the NRD waveguide hybrid IC, and components in which the resonance characteristic was important were made with waveguide. An amplifier packaged and modularizing the bare chip, it equipped at the NRD waveguide within. Manufactured transceiver communicated with FDD method, and it had 10dBm output power, and -60dBm minimum receive sensitivity.

High Efficiency V-band Power Combining Modules Using Slotline-to-Microstrip Transition (슬롯라인-마이크로스트립 변환을 이용한 고효율 V-band 전력 결합 모듈)

  • Kim Dong-Ki;Jeong Jin-Ho;Kwon Young-Woo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.16 no.6 s.97
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    • pp.580-585
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    • 2005
  • Two high-efficiency and high power power-combing modules were developed using slotline-to-microstrip transition at V-band. Power-combining modules incorporating two MMIC power amplifiers demonstrated combining efficiencies higher than $80\%$(maximum $86\%$) with saturated output power of 22.96 dBm and 22.81 dBm, respectively. The measurement of back-to-back connected combiners demonstrated insertion loss less than 1.2 dB with return loss better than 15 dB around 60 GHz, respectively.

Quality Characteristics of Syrup made with Saccharified Barley Liquid (보리당화액을 첨가한 시럽의 제조와 품질특성)

  • Kim, Ji-Hyun;Cho, Eun-Ju
    • Culinary science and hospitality research
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    • v.21 no.6
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    • pp.242-254
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    • 2015
  • Barley is a main food source, along with rice, in our dietary life that is easy to buy and process. It is required to develop a beverage base and barley syrup for desserts in order to raise utilization of barley beyond its present use in Sikhye (sweet fermented rice drink) and Jocheong (grain syrup) production. In pursuit of the goal to increase the usability of barley as an ingredient of processed food, this study examined the optimal preparation conditions of barley mash for barley syrup. In addition, the study prepared a barley syrup using saccharified barley liquid, analyzed quality characteristics, and conducted a sensory evaluation. Saccharified barley liquid(barley mash) was prepared with ratios of adding malt of unhulled barley germinated as 0 g(CON), 10 g(BM10), 20 g(BM20), and 30 g(BM30). The results of measuring pH under the varied conditions of saccharification temperatures from $50^{\circ}C$, $60^{\circ}C$, and $70^{\circ}C$, as well as time from 1 to 8 hours, treveal that the optimum condition for malt saccharification is $60^{\circ}C$ for 5 hours. By adding oligosaccharides and lemon juice to the saccharified barley liquid at the different ratios of added malt, this study measured quality characteristics(color value, viscosity, pH, sweetness) of barley syrup warmed up for 1 hour. As the result of measuring color in the barley syrup prepared by saccharified barley liquid, higher levels of added malt saw L-value decreased while a-value and b-value both increased. BMs30 showed the highest viscosity of $1,202.67{\pm}3.06$. As for pH, BMs30 was the highest at pH $3.57{\pm}0.02$. The result of the sensory evaluation of barley syrup showed the superior sensory characteristics of BMs20 in terms of color, flavor, sweetness, viscosity and overall quality.

Dual-Band Class-F Power Amplifier based on dual-band transmission-lines (이중 대역 전송선로를 활용한 이중 대역 F급 전력 증폭기 개발)

  • Lee, Chang-Min;Park, Young-Cheol
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.47 no.4
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    • pp.31-37
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    • 2010
  • In this paper, highly efficient dual-band class-F power amplifiers(PAs) for cellular and WLAN bands are suggested and implemented. For the first step, single-band class-F amplifiers at 840MHz, 2.4GHz are designed using commercial E-pHEMT FETs. The performance of two single band PAs are as much as 81.2% of efficiency with the output power of 24.4dBm with 840MHz PA and 93.5% of efficiency with 22.4dBm from the 2.4GHz. For the dual-band class-F PA, the harmonic controlling circuit with ideal SPDT switch was suggested. The length of transmission line is variable by a SPDT switch. As a results, the operation in 840MHz showed the peak efficiency of 60.5% with 23.5dBm, while in 2.4GHz mode the efficiency was 50.9% with the output power of 19.62dBm. Besides, it is shown that the harmonic controller of class-F above 2Ghz could be implemented on the low cost FR-4 substrate.

The study on dynamic fracture toughness of friction-welded M.E.F. dual phase steel (복합조직강의 마찰용접부에 대한 동적파괴특성)

  • 오세욱;유재환;이경봉
    • Journal of Welding and Joining
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    • v.7 no.3
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    • pp.19-27
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    • 1989
  • Both the SS41 steel and the M.E.F(martensite encapsulated islands of frrite) dual phase steel made of SS41 steel by heat treatment were welded by friction welding, and then manufactured machinemade Vnotch standard Charpy impact specimens and precracked with a fatigue system at BM(base metal), HAZ(heat affected zone) and WZ(weld interface Zone). The impact test of them was performed with an instrumented impact test machine at a number of temperatures in constant loading velocity and the dynamic fracture characteristics were studied on bases of the absorbed energy, dynamic fracture toughness and fractography from the test. The results obtained are as follows; At the room temperature, the absorbed energy is HAZ.geq.WZ.geq.BM in case of the M.E.F. dual phase steel: BM.geq.HAZ.geq.WZ in case of the SS41 steel, HAZ.geq.BM.geq.WZ at the low temperature. The absorbed energy is decreased markedly with the temperature lowering; it is highly dependent on the temperature. The dynamic fracture toughness of the M.E.F. dual phase steel is HAZ.geq.WZ.geq.BM at the room temperature; BM.geq.WZ.geq.HAZ below-60.deg. C. Therefore the reliability of friction welding is uncertain at the low temperature(below-60.deg. C). The dynamic fracture toughness of the SS41 steel; HZA.geq.WZ.geq.BM at overall temperature region. The flaw formed by rotational upsetting pressure was shown y SEM; in this region. The absorbed energy per unit area and dynamic fracture toughness were low relative to other region.

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Design and Fabrication of a Broadband RF Module for 2.4GHz Band Applications (2.4GHz 대역에서의 응용을 위한 광대역 RF모듈 설계 및 제작)

  • Yang Doo-Yeong;Kang Bong-Soo
    • The Journal of the Korea Contents Association
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    • v.6 no.4
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    • pp.1-10
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    • 2006
  • In this paper, a broadband RF module is designed and tested for 2.4GHz band applications. The RF module is composed of a low noise amplifier (LNA) with a three stage amplifier, a single ended gate mixer, matching circuits, a hairpin line band pass filter and a Chebyshev low pass filter to convert the radio frequency (RF) into the intermediate frequency (IF). The LNA has a high gain and stability, and the single ended gate mixer has a high conversion gain and wide dynamic range. In the analysis of the broadband RF module, the composite harmonic balance technique is used to analyze the operating characteristics of an RF module circuit. The RF module has a 55.2dB conversion gain with a 1.54dB low noise figure, $-120{\sim}-60dBm$ wide RF power dynamic range, -60dBm low harmonic spectrum and a good isolation factor among the RF, IF, and local oscillator (LO) ports.

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