• 한국해양생명과학회지
• /
• 제6권2호
• /
• pp.97-105
• /
• 2021

꼬막은 해양 어업으로써 아시아 전 지역에 있어서 중요한 수산자원 중 하나이다. 하지만, 공장의 산업화, 해양 환경오염, 그리고 지구 온난화로 인해 해양 어업 생산량이 급격히 떨어졌다. 우리나라 남해안의 주요 수산자원인 꼬막의 유전적 특성을 파악하기 위하여 꼬막의 전장유전체를 해독하고 염색체 서열을 규명하였다. 915.4 Mb의 게놈을 조립하였고, 19개의 염색체 유전자 서열을 식별하였다. 꼬막의 유전체에서 25,134개의 유전자들을 확인하였고, 그 중에 22,745개의 유전자들에 대한 기능을 확인했으며, 4,014개의 유전자들에 대한 KEGG pathway를 분석하였다. 꼬막유전체와 8종의 다른 패류와 비교유전체 분석을 통하여 확장/감소(gene gain and loss) 분석을 수행한 결과, 725개의 유전자군의 확장과 479개의 유전자군의 감소를 확인하였다. 꼬막의 homeobox 유전자 클러스터는 촉수담륜동물 내에서 잘 보존된 유전자 구조를 보였다. 또한, 꼬막은 3개의 hemoglobin 유전자들이 피조개의 hemoglobin과 높은 유사성을 보였다. 꼬막의 전장유전체 정보를 통해 꼬막의 환경 적응과 진화의 유전적 특성과 생리적 특성뿐만 아니라, 꼬막 양식의 효율성을 높이는 양식산업에 널리 이용될 수 있는 유전적 정보를 제공할 것이다.

• 대한의생명과학회지
• /
• 제11권1호
• /
• pp.85-88
• /
• 2005

Hemoglobin is oxygen carrier protein within erythrocyte in blood. Apoprotein of this, globin, is synthesized in the cytosol but it's cofactor, heme, is synthesized in the mitochondria. It has not been known very well how globin receives the heme from mitochondria and folds to hemoglobin. In this folding process, the initial structure of globin seems to be very important. A small volume of globin at acid pH was added rapidly into the bulk of an egg phosphatidylcholine $60\%$ liposome, containing hemins, at neutral pH according to the Rapid Dilution method. It was observed that an acid-induced unfolding structure of globin is initially needed to receive hemins from the lipid bilayer of liposomes. Also, this conclusion was confirmed with the absorption spectrum of the refolded globin separated by centrifugation.

• 대한의생명과학회지
• /
• 제23권3호
• /
• pp.208-214
• /
• 2017

To investigate the effects of strenuous physical exercise on commonly used hematological markers in subjects the intensive long running. Blood samples were obtained from nineteen participants in a 622 km ultra-marathon race before, 300 km and immediately after completion of the 622 km ultra-marathon. Samples were analyzed for total white cell count (WBC) and differential, total red cell count (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), platelets, mean platelets volume (MPV), platelets distribution width (PDW). Significant increases were found in WBC, neutrophil and platelets at 622 km compared to the pre-race. RBC, hemoglobin and hematocrit decreased statistically significantly the race at 300 km and 622 km compared to pre-race. A wide range of hematological perturbations occur during 622 km ultra-marathon running but it was physiological changes within a reference range. The 622 km ultra-marathon is less likely to cause clinically significant hematologic changes in athletes.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
• /
• pp.208.2-208.2
• /
• 2016

Many researchers have paid attention to the studies on the interaction between non-thermal plasma and aqueous solutions for biomedical applications. The gas composition in the plasma is very important. Oxygen and nitrogen are the main gases of interest in biological applications. Especially, we focus on the oxygen concentration. In this experiment, we studied the role of oxygen concentration in plasma induced chemical reactions in solution. At first, the amount of ions are measured according to changing the oxygen concentration. And we checked the relationship between these ions and pH value. Secondly, when the oxygen concentration is changed, it identified the type and amount of radical generated by the plasma. In order to confirm the effect of these chemical property change to biological material, hemoglobin and RBCs are chosen. RBCs are one of the common basic biological cells. Thirdly, when plasma treated according to oxygen concentration in nitrogen feeding gas, oxidation of hemoglobin and RBC is checked. Finally, membrane oxidation of RBC is measured to examine the relation between hemoglobin oxidation and membrane damage through relative hemolysis and Young's modulus. Our results suggest that reactive species generated by the plasma differsdepending on the oxygen concentration changes. The pH values are decreased when oxygen concentration increased. OH decrease and NO increase are also observed. These reactive species makes change of chemical properties of solution. We also able to confirm that the difference in these reactive species to affect the oxidation of the Hb and RBCs. The Hb and RBCs are more oxidized with the high oxygen concentration conditions. But membrane is damaged more by plasma treatment with only nitrogen gas. It is shown that red blood cells membrane damage and oxidation of hemoglobin are not directly related.

• 대한수의사회지
• /
• 제26권1호
• /
• pp.38-42
• /
• 1990

The effect of Aloe vera administration(500mg/kg, P.O.) for 21 days on the total white blood cell, packed cell volume, hemoglobin, total protein, serum glutamic pyruvic transaminase and sorbitol dehydrogenase values in cobalt-60 gamma irradiated rats was s

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제37권4호
• /
• pp.272-277
• /
• 2011

Purpose: The aim of this study was to compare the estimated blood loss and determine the change in hemoglobin depending on the combination of each orthognathic surgery. Subjects and Methods: The subjects of this study were patients who underwent orthognathic surgery among those diagnosed with a dentofaical deformity in Mok-Dong hospital, Ewha Womans University from 2002 to 2009. One hundred patients (men - 36, women - 64, mean age of $24.5{\pm}4.6$) participated in the study and were divided into four groups (group 1 - bilateral sagittal ramus osteotomy [BSSRO], group 2 - BSSRO+Genioplasty, group 3 - Lefort 1+BSSRO+genioplasty, group 4 - anterior segmental osteotomy on maxilla and mandible). A comparative study on the estimated blood loss (EBL), operation time, peri-operative changes in hemoglobin was performed using anesthesia records. The results were analyzed statistically using a Mann-Whitney U-test and Spearman's Rho test - SPSS 12.0 (SPSS Inc. Chicago, IL, USA). Results: In group 1 (BSSRO), the mean EBL, operation time and change in hemoglobin was $394.43{\pm}52.69$ ml, $184{\pm}42.33$ minutes, and 1.43, respectively, In group 2 (BSSRO+genioplasty), it was $556.32{\pm}63.42$ ml, $231{\pm}37.45$ minutes, and 1.80, respectively. In group 3 (Lefort 1+BSSRO+Genioplasty), it was $820.55{\pm}105.54$ ml, $320{\pm}15.41$ minutes, and 2.73, respectively. In group 4 (segmental osteotomy), it was $1025.39{\pm}160.21$ ml, $355{\pm}20.10$ minutes, and 3.33, respectively. In particular, in group 3, significant differences were observed depending on the method of the orthognathic surgery. The mean EBL in a Lefort 1 osteotomy with advancement was only 687 ml, whereas Lefort 1 osteotomy with canting correction (992 ml), even impaction (764 ml), and posterior nasal spine impaction (100 ml) showed a much higher EBL. Conclusion: From these results, the EBL and peri-operation hemoglobin increased as treatment plans became more complicated and increasing operation time. Safe orthognathic surgery should be performed by applying proper autologous transfusion plans based on the average EBL of each orthognathic surgery type.

• 한국어병학회지
• /
• 제6권2호
• /
• pp.123-131
• /
• 1993

1990년 8월에 양식넙치의 병어에서 분리한 Staphylococcus epidermidis를 건강한 넙치에 인위감염 시킨 후, 혈액성상과 혈청화학성분의 변화를 24시간 간격으로 4일간 추적하였다. 적혈구수, 헤모글로빈량, 헤마토크릿치, 평균적혈구헤모글로빈농도 및 평균적혈구헤모글로빈량은 균 접종 24시간에서 48시간 내에 유의적으로 감소하였다. 총단백, 알부민, 글로부린 및 총콜레스테롤치는 균 접종 24시간에서 45시간만에 유의적으로 증가하였으나 혈당, 빌리루빈 및 혈청 트란스아미나제는 균 접종 24시간에서 48시간내에 유의적으로 감소하였다. 균 접종 48시간 후에 적혈구는 장경이 짧게 되어 둥근 모양으로 되었다. 균 접종 72시간 후에는 용혈된 적혈구와 호중구가 많이 출현하였다. 균 접종 48시간 후부터 프라이스-존즈 곡선은 좌방으로 이행되었다. 따라서 인위적 감염에 의한 양식 넙치의 포도상구균증은 용혈성 빈혈을 나타내었다.

• 한국식품영양과학회지
• /
• 제19권1호
• /
• pp.53-60
• /
• 1990

농촌 주부의 식생활상태는 건강과 밀접한 관계를 가지므로 식생활환경과 식사내용 및 혈액 성상에 대한 내용을 분석 비교하였다. 대상자의 학력은 낮으며 가족수는 전국평균보다 많으며 약한 저체중상태가 60%나 되었다. 가정기기의 사용률은 중간 정도이나 농기계 보유율은 1.36대에 불과 하였으며 위생생활은 이루어진다고 볼 수 있으나 문화생활수준은 매우 낮았다. 혈압은 정상보다 낮으며 농부증은 4.72점으로서 요관찰대상점수였다. 식품군별 섭취량은 전반적으로 낮았으며 곡류와 채소가 주류이고 동물성식품은 빈약하였다. 영양소는 비타민C 및 아니아신을 제외하고 모두 권장량에 미치지 못하였으며 칼슘은 66.3%, 철분은 56.6%로서 특히 낮았다. 혈액성상은 혈청단백질은 정상으로 지질, 칼슘, 마그네슘 등은 정상범위는 벗어나지 않으나 낮은 수준이었다. 혈청철분과 헤모글로빈, 헤마토크릿은 낮은 수치가 많아 빈혈빈도가 높음을 보였으며 혈청 ferritin은 특히 낮았다. 각 요인의 상관관계를 보면, 곡류를 혈청마그네슘과 높은 상관을 보였고 혈청콜레스테롤은 곡류, 유지류, 채소류, 육류의 섭취와 상관을 보이고 체중과 신체충실지수와 밀접한 상관을 보였다. 빈혈과 관련하여 볼 때 헤모글로빈과 헤마토크릿 수치는 과일류, 생선류의 섭추와는 음의 상관을, 신체충실지수와는 양의 상관을 보였다.

• Childhood Kidney Diseases
• /
• 제22권2호
• /
• pp.42-46
• /
• 2018

Purpose: We investigated whether the white blood cell (WBC) count to hemoglobin (Hgb) level ratio is correlated with the presence of cortical defects on dimercaptosuccinic acid (DMSA) renal scan in children with febrile urinary tract infection (UTI). Methods: We examined 95 children who were consecutively admitted to our hospital with their first episode of febrile UTI. Blood tests (C-reactive protein [CRP], WBC, Hgb] were performed. All enrolled children underwent DMSA scanning during admission. Data were compared between children with positive and negative DMSA results. The correlations between WBC to Hgb ratio and the presence of cortical defects on DMSA scan, and between WBC to Hgb ratio and CRP level were analyzed using the Pearson chi-squared test. Multiple logistic regression analysis was used to evaluate whether WBC to Hgb ratio could predict the cortical defects on DMSA scan in children with febrile UTI. Results: The WBC to Hgb ratio was significantly higher in children with positive DMSA results than in those with negative DMSA results; positively correlated with the presence of cortical defects on DMSA scan and CRP; and was a significant factor for predicting the presence of cortical defects on DMSA scan. Conclusion: The WBC to Hgb ratio may predict the presence of cortical defects on acute DMSA scans in children with febrile UTI.

• 한국컴퓨터정보학회논문지
• /
• 제23권10호
• /
• pp.135-141
• /
• 2018

In this paper, we propose to evaluate the effect of resistin-like molecule alpha ($Relm{\alpha}$) on the progression of anemia of inflammation. Anemia of inflammation is a common feature of inflammatory disorders, including chronic kidney disease, infections, and rheumatoid arthritis. $Relm{\alpha}$ is highly up-regulated in various inflammatory states, especially those involving asthma, intestinal inflammation, and parasitic diseases, and regulates the pathogenesis of those diseases. However, the role of $Relm{\alpha}$ in anemia of inflammation is unknown. To explore the roles of $Relm{\alpha}$ in anemia of inflammation in vivo, we generated mouse model of the disease by injecting 0.25 mg/kg lipopolysaccharides (LPS) intraperitoneally into $Relm{\alpha}-deficient$ and wild-type (WT) mice daily for 10 days. Research data was expressed as differences between LPS-treated $Relm{\alpha}-deficient$ and WT mice by a two-tailed non-parametric Mann-Whitney U-test using GraphPad Instat program. The results of the study are as follows: LPS-treated $Relm{\alpha}-deficient$ mice had significantly (p<0.05) lower hemoglobin contents, hematocrit levels and red blood cell indices including mean corpuscular volume, mean corpuscular hemoglobin than WT controls. This decrease was accompanied by significant (p<0.05) increase in total white blood cell and monocyte counts in the blood. However, there was no significant difference in mRNA levels of hepatic hepcidin and renal erythropoietin between the two animal groups. Taken together, these results indicates that $Relm{\alpha}$ deficiency exacerbates the anemia by increasing inflammation, suggesting therapeutic value of $Relm{\alpha}$ in the treatment of anemia of inflammation.