• 디지털융복합연구
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• 제15권5호
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• pp.471-478
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• 2017

본 연구에서는 제주 해녀를 대상으로 식이성 톳 섭취가 혈압 및 총콜레스테롤 변화에 미치는 영향에 대해 알아보고자 하였다. 20명의 현직해녀지원자를 10명씩 나누어 톳 섭취 집단과 톳을 섭취하지 않을 집단으로 구분한 후, 8주 간톳 섭취 집단에게 일일 100g의 식이성 톳을 밥과 함께 섭취케 하여 차이를 비교하였다. 그 결과 첫째, 톳을 섭취한 집단의 수축기 혈압은 유의하게 감소하였다. 둘째, 이완기 혈압은 두 집단 모두 유의한 감소를 보였으며, 집단 간 차이는 없었다. 셋째, 수축기-이완기 혈압 차이는 톳을 섭취한 집단은 유의한 감소를 보였으며, 톳을 섭취하지 않은 집단은 증가하였다. 넷째, 총콜레스테롤은 톳을 섭취한 집단에서 유의한 감소가 나타났지만 톳을 섭취하지 않은 집단은 증가하였다. 이상의 결과는 톳을 일일 100g씩 밥과 함께 섭식함으로써 총콜레스테롤 감소 및 수축기 혈압 감소에 효과가 있었음을 나타낸다.

• BMB Reports
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• 제36권6호
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• pp.565-571
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• 2003

The locomotor responses of human peripheral blood neutrophils and lymphocytes were measured by the change from spherical to polarized shapes in the presence of endotoxins (lipopolysaccharide, LPS) of enteric pathogens: S. dysenteriae type 1, V. cholerae Inaba 569B, S. typhimurium, and K. pneumoniae. We reported earlier that these endotoxins are chemotactic factors for the neutrophils since they stimulated cell polarization within a few minutes of incubation. Endotoxins had an inhibitory effect upon neutrophil phagocytosis of opsonized yeast and the cells engulfed fewer yeasts. Interestingly, endotoxins increased neutrophil adhesion to clean glass surfaces, but stimulated the cells to exhibit increased random locomotion (chemokinesis) through cellulose nitrate filters and show an enhanced ability to reduce nitroblue tetrazolium (NBT) dye. Unlike neutrophils, lymphocytes direct from blood do not show polarized morphology towards chemotactic factors but the cells acquire locomotor capacity during 24-72 h culture with mitogens such as phytohemagglutinin (PHA), phorbol myristate acetate or concanavalin A. Stimulation of blood lymphocytes with endotoxins did not induce cell polarization in short-term but long-term culture resulted in an increase in the proportion of polarized cells that acquired locomotor morphologies. The majority of these cells were identified as esterase negative B-lymphocytes that migrated through filters. Despite the optimum time of incubation for each of these cell types being different, we found that lymphocytes respond to much lower concentrations of endotoxins than the neutrophils. These findings suggest that endotoxins of enteric pathogens modulate the functions of human blood neutrophils and lymphocytes.

• 비교문화연구
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• 제21권
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• pp.165-188
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• 2010

One of the things that should be done first in establishing the cosmic life culture is to reflect on the old human-oriented culture. If the cosmic life culture absolutizes everything except for humans according to the logic of reason, its foundation will weaken or eventually get lost. Separating humans from the universe is just like separating life from it. Given that all life, whether it is humans or the earth, originated from the universe, such an effort for separation merely values an individual life by excluding all life or whole life. When the human body and the cosmic chi' blood are not in an active flow, it means there is a problem with life. What is in the greatest need in such a case is the sincere human mind that follows the principle of cosmic life. It is like the sincerity found in the pasonri singer, who mellows all the hardships and difficulties in the world out and create songs out of them like the shadow. It is the pansori singer's shadow that changes the universe. It is only when the extreme force of human mind communicates with that of the universe that the cosmic life or cosmic life culture can be created. In that sense, it is urgent to create life out of the universe inside me and create a universe out of all life in and outside me. It is such a grave plan in human history in that it involves finding the "Sanal" which is the core of life living hidden inside the body whose life force gradually goes away or inside the universe, and creating the culture of Bokseung in which it bursts out. The most important thing in life is the flow, and the mankind is currently standing in the life flow of the massive universe's chaosmos. The greatest task the mankind is currently faced with is to think over how to deal with the period of Big Chaos in the massive universe's chaosmos reversely and establish the cosmic life culture anew.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1433-1442
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• 2021

In this study, Saccharomyces cerevisiae culture fluid (SCCF) has been added to a diet of lactating dairy cows to attempt to improve the ruminal fermentation and potentially increase the dry matter intake (DMI) and milk yield. This study was conducted to investigate the effects of SCCF on the milk yield and blood biochemistry in lactating cows during the summer. Twenty-four Holstein dairy cows were randomly assigned to one of four treatments: (1) total mixed ration (TMR-1) (Control); (2) TMR-1 supplemented with SCCF (T1); (3) TMR-2 (containing alfalfa hay) (T2); and (4) TMR-2 supplemented with SCCF (T3). SCCF (5 ml/head, 2.0×10⁷ CFU/mL) was mixed with TMRs daily before feeding to dairy cows. The mean daily temperature-humidity index (THI) during this trial was 76.92 ± 0.51 on average and ranged from 73.04 to 81.19. For particle size distribution, TMR-2 had a lower >19 mm fraction and a higher 8-9 mm fraction than TMR-1 (p < 0.05). The type of TMR did not influence the DMI, body weight (BW), milk yield and composition, or blood metabolites. The milk yield and composition were not affected by the SCCF supplementation, but somatic cell counts were reduced by feeding SCCF (p < 0.05). Feeding SCCF significantly increased the DMI but did not affect the milk yield of dairy cows. The NEFA concentration was slightly decreased compared to that in the control and T2 groups without SCCF. Feeding a yeast culture of S. cerevisiae may improve the feed intake, milk quality and energy balance of dairy cows under heat stress.

• 문화기술의 융합
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• 제6권3호
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• pp.413-418
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• 2020

신경통은 중증의 고통을 수반하는 질병으로써 인간의 삶의 질에 매우 강한 영향을 주는 질병이며, 국내에서도 노화에 따라 유병율이 증가하는 추세이다. 그동안 신경통에 대한 국내외연구들은 주로 신경통과 연관성이 있는 정신질환, 인구학정 정보, 영양성분 등 과의 연관성 분석이 대부분이고, 혈액정보와의 연관성 연구는 매우 드물다. 따라서, 본 연구에서는 신경통과 혈액 파라미터들과의 연관성을 도출하고 신경통과 연관성이 있는 임상지표를 도출하고자 한다. 본 연구를 위해 질병관리본부의 국민건강영양조사 데이터를 기반으로 binary logistic regression을 이용하여 분석하였다. 실험결과 남녀 모두에서 나이가 신경통과 가장 연관성이 높은 경향이 있는 것으로 나타났으며, 여성에서는 복부둘레와 헤마토크리트 수치가 신경통과 연관성이 있는 것으로 나타났고, 남성에서는 공복시혈당과 헤모글로빈이 신경통과 연관성이 있는 것으로 나타났다. 또한 복부둘레 및 혈액정보들과 신경통사이에서의 연관성이 남녀에서 차이가 있는 것을 밝혀내었다.

• 한국식생활문화학회지
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• 제34권6호
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• pp.748-760
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• 2019

This study examined the difference of dietary intake behavior between groups of adults aged >20 years with or without dyslipidemia, and comparing the various types of dyslipidemia, based on the Seventh (2016) KNHANES. Men showed higher rate for triglyceridemia and LDL-cholesterolemia than women and subjects in the age group 50-59 years exhibited the highest incidence of triglyceridemia, total cholesterolemia and HDL-cholesterolemia. The ratio of obese people was higher in most types of dyslipidemias. The top five foods in each group of cereal & grain, meat, vegetable, and fruit were selected for analysis, based on the food frequency. The dyslipidemia group showed higher intake frequencies for mixed grains, cabbage kimchi and leafy vegetable than the no-dyslipidemia group, and the group with high blood total cholesterol for mixed grains, apple, and cutlassfish-croaker. Group with high blood triglyceride showed higher intake frequencies of rice and mackerel-mackerel pike than the other groups of high blood triglyceride. No food showed significant difference in the frequencies between groups of LDL-cholesterol. Group with high blood HDL-cholesterol showed higher intake frequency of bibim-fried rice, deep fried chicken, stir fried chicken, apple, tangerine, banana and butter-margarine. These results indicate that people with high levels of blood total cholesterol are more concerned with food having health benefits than those with abnormal levels of other blood lipid. More information on dietary benefits need to be provided to patients having high levels of triglyceride, LDL-cholesterol and HDL-cholesterol.

• 한국식생활문화학회지
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• 제24권3호
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• pp.338-343
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• 2009

This study was conducted to investigate the effects of Takju lees hot water extracts on the blood pressure in spontaneously hypertension rats (SHR). Twenty eight male SHR were grouped by their blood pressure and fed a control diet or experimental diets containing 1% (G1), 2% (G2), or 4% (G4) Takju lees extracts for 4 weeks. Food intake was not significantly different among the groups. However, body weight gain was significantly lower in groups fed the Takju lees extract than the control group. The systolic blood pressure was significantly lower in the Takju lees extract containing groups (especially in G4 group) than the control groups. In addition, mean blood pressure {(systolic+dyastolic)/2} decreased with an increase in the amount of Takju lees extract in the diet and feeding period. Takju lees extract decreased angiotensin I converting enzyme (ACE) activity in a dose-dependent manner. These results suggest that the Takju lees extract exert an antihypertensive effect by decreasing ACE activity.

• 한국식생활문화학회지
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• 제21권2호
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• pp.216-224
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• 2006

This study was conducted to examine the correlation among meals variety, obesity index, blood pressure, and lipid profiles of Korean adults. A total of 308 adults(men 124, women 184) measured the anthropometric characteristics, blood pressure, dietary intake using 24-hour recall method, and serum lipid profile. The average ages of the study subjects were 50.9 yrs in men and 51.4 yrs in women. The average height, weight and BMI were 166.3 cm, 68.4 kg, $24.7\;kg/cm^2$ in men and 154.3 cm, 59.8 kg, $25.0\;kg/cm^2$ in women, respectively. The numbers of food items in meal of men and women were 14.1, 15.9 for breakfast, 15.7, 15.9 for lunch, 14.7, 14.1 for dinner, respectively. The numbers of dish items in meal of men and women were 4.0, 4.3 for breakfast, 4.3, 4.3 for lunch, 4.2, 3.9 for dinner, respectively. The average blood pressure, total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol and atherogenic index were 126.6/76.0 mmHg, 181.0 mg/dL, 157.7 mg/dL, 40.9 mg/dL, 108.6 mg/dL, 3.5 in men and 123.2/73.6 mmHg, 185.0 mg/dL, 137.3 mg/dL, 44.8 mg/dL, 112.7 mg/dL, 3.2 in women, respectively. The total cholesterol was negatively correlated to the number of food item for lunch(p<0.05). Therefore, more systematic studies to investigate the meal variety roles of blood lipids in meals of adults were required.

• 대한미생물학회지
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• 제11권1호
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• pp.27-32
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• 1976

Sodium amylosulfate(SAS) has been reported to be an effective substance to inactivate the anti-bacterial activity of blood in blood culture media. The advantage of the use of SAS over sodium polyanethol sulfonate(SPS) is that it does not inhibit the growth of some bacteria! species which are known to be inhibited by SPS. As to S. typhi, SPS is reported to enhance the growth, however the effect of SAS on this organism is not known as yet. Using 43 strains of S. typhi, isolated from clinical materials, the authors tried to determine the effect of SAS on this organism. The methods used for this study were : the SPS and SAS paper disk I sensitivity test, tests on the growth in trypticase soy broth(TSB) with SPS and with SAS, and experimental blood culture in SPS and SAS incorporated TSB. The following results were obtined. 1). S. typhi strains with the turbidity of No. 0.5 tube of MacFarland nepherometer were inoculated onto Mueller-Hinton plate and 1mg disk of SPS and SAS were applied. After 24-hour incubation, none of the 43 strains showed inhibition zone by SPS disk, but all of them showed zones by SAS disk with a mean zone diameter of 9.5mm(Table 1). 2) Inocula consisting of one to 54 viable counts of 37 strains were inoculated into three different media; TSB with 0.05% SPS, TSB with 0.05% SAS and TSB alone. After 24-hour incubation the mean of the optical densities of each medium were 0.483, 0.482 and 0.459 respectively, showing that SAS does not inhibit the growth of S. typhi. Moreover it was shown that there was no correlation between the amount of inocula and growth(Table 2 and Fig. 1). 3). Each set of media in 5 ml amounts consisting of one tube of TSB with 0.05% SPS, one tube of TSB with 0.05% SAS and two tubes of TSB were inoculated with 8, 64. 640 and 6400 viable counts of bacteria. Then 0.5 ml of fresh normal blood was added to all tubes except for one tube of TSB. Macroscopic observation after 24 hour incubation showed a heavy growth in all tubes except for the tube of TSB plus blood, which showed only a light growth in the tube of the heaviest inoculum. This result clearly demonstrates that the growth of S. typhi is inhibited by some antibacterial activities of fresh blood, which are counter acted by SPS and SAS(Table 3). Between SPS and SAS, there was no significant difference found(Table 4 and Fig. 2). With all these results it can be postulated that the addition of SAS into a rountine blood culture media may raise the positivity of S. typhi isolation and shorten the incubation period.

• Clinical and Experimental Pediatrics
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• 제48권8호
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• pp.894-900
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• 2005

목 적 : 조직적합 항원의 불일치로 인하여 골수이식을 할 수 없는 경우에 점점 더 제대혈이 사용되고 있다. 그러나 제대혈의 조혈모세포의 수가 적기 때문에 이를 증가시킬 대책이 필요한 바, 여러 성장인자를 조합하여 체외증폭하여 말초혈의 체외증폭과 비교하였다. 방 법 : 저자들은 제대혈 및 말초혈로부터 분리한 CD34+ 세포를 혈청이 아닌 배양체에서 체외 증폭하여 비교하였다. Miltenyi 방법으로 분리한 CD34+는 조혈성장인자들과 함께 체외 증폭 시켰다. 증폭 당일, 4일 후, 7일 후 및 14일에 증폭된 세포를 가지고 burst-forming units of erythrocytes (BFU-E), colony-forming units of granulocytes and monocytes (CFU-GM) 및 colony-forming units of megakaryocytes (CFU-Mk)의 생성 능력을 알아보았다. 결 과 : 말초혈에 비하여 제대혈로부터 분리한 CD34+ 세포의 증폭 능력이 2배로 컸다. 체외에서7일 및 14일 동안 증폭된 제대혈이 더 많은 BFU-E를 생성하였고, 4일 및 7일 동안 증폭된 제대혈이 더 많은 CFU-Mk를 생성하였다. 결 론 : MGDF, FL 및 IL-3를 포함한 성장인자의 자극 하에서 제대혈의 체외 증폭이 더 많은 BFU-E 및 CFU-Mk를 생성하였으므로, 이를 이용한 체외 증폭을 시도하는 것의 가능성을 시사하고 있다.