Browse > Article

Ex vivo Expansion and Clonal Maintenance of CD34+ Selected Cells from Cord Blood and Peripheral Blood  

Kim, Soon Ki (Hematology-Oncology, Department of Pediatrics, Inha University of Hospital, Inha Medical College)
Ghil, Hye Yoon (Clinical Research Center, Inha University of Hospital, Inha Medical College)
Song, Sun U. (Clinical Research Center, Inha University of Hospital, Inha Medical College)
Choi, Jong Weon (Department of Laboratory Medicine, Inha University of Hospital, Inha Medical College)
Park, Sang Kyu (Department of Pediatrics§, College of Medicine, Ulsan University)
Publication Information
Clinical and Experimental Pediatrics / v.48, no.8, 2005 , pp. 894-900 More about this Journal
Abstract
Purpose : Because of the unavailability of marrow transplantation, umbilical cord blood (CB) is increasingly being used. We evaluated the potential of ex vivo expansion and clonality in CD34+ cells separated from cord blood source and mobilized peripheral blood (PB) in a serum-free media. Methods : The CD34+ cells, selected from CB and mobilized PB, were expanded with hematopoietic growth factors. They were then cultured for burst-forming units of erythrocytes (BFU-E), colony-forming units of granulocytes and monocytes (CFU-GM) and colony-forming units of megakaryocytes (CFU-Mk) at culture days 0, day 4, day 7, and day 14 with various growth factors. Results : The CB-selected CD34+ cells showed significantly higher total cell expansion than those from the PB at day 7 (2 fold increase than PB). The CB-selected CD34+ cells produced more BFU-E colonies than did the PB on culture at days 7 and at day 14. Also, the CB-selected CD34+ cells produced more CFU-Mk colonies than did the PB on culture at day 4 and at day 7. Conclusion : The ex vivo expansion of the CB cells may be promising in producing total cellular expansion, CFU-Mk and BFU-E compared with PB for 7 to 14 days. The growth factors combination including megakaryocyte growth and development, flt3-ligand and interleukin-3 showed more expansion in the view of total cells and clonal maintenance compared with less combination.
Keywords
CD34+ cells; Colony-forming unit of megakaryocyte; Cord blood; Mobilized peripheral blood; Ex vivo expansion;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kaushansky K, Lok S, Holly RD, Broudy VC, Lin N, Bailey MC, et al. Promotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombopoietin. Nature 1994;369:568-71   DOI   ScienceOn
2 Miltenyi S, Muller W, Weichel W, and Radbruch A. High gradient magnetic cell separation with MACS. Cytometry 1990;11:231-8.   DOI   ScienceOn
3 Traycoff CM, Abboud MR, Laver J, Brandt JE, Hoffman R, Law P, et al. Evaluation of the in vitro behavior of phenotypically defined populations of umbilical cord blood hematopoietic progenitor cells. Exp Hematol 1994;22:215-22
4 McKenna HJ, de Vries P, Brasel K, Lyman SD, Williams DE. Effect of flt3 ligand on the ex vivo expansion of human CD34＋ hematopoietic progenitor cells. Blood 1995; 86:3413-20
5 Bernstein ID, Andrews RG, Zsebo KM. Recombinant human stem cell factor enhances the formation of colonies by CD34＋and CD34＋lin- cells, and the generation of colonyforming cell progeny from CD34＋lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor. Blood 1991;77:2316-21
6 Goussetis E, Theodosaki M, Paterakis G, Stefanaki K, Tsecoura C, Papassarandis C, et al. Kinetics of quiescent cord blood stem/progenitor cells with high proliferative potential in stem-cell expansion culture. Cytotherapy 2003; 5:500-8   DOI   ScienceOn
7 Debili N, Wendling F, Katz A, Guichard J, Breton-Gorius J, Hunt P, et al. The Mpl-ligand or thrombopoietin or megakaryocyte growth and differentiative factor has both direct proliferative and differentiative activities on human megakaryocyte progenitors. Blood 1995;86:2516-25
8 Hirao A, Kawano Y, Takaue Y, Suzue T, Abe T, Sato J, et al. Engraftment potential of peripheral and cord blood stem cells evaluated by a long-term culture system. Exp Hematol 1994;22:521-6
9 Moore MAS, Hoskins I. Ex vivo expansion of cord bloodderived stem cells and progenitors. Blood Cells 1994;20:468-81
10 Young JC, Bruno E, Luens KM, Wu S, Baker M, Murray LJ. Thrombopoietin stimulates megakaryocytopoiesis, myelopoiesis, and expansion of CD34＋ progenitor cells from single CD34＋Thy-1＋Lin- primitive progenitor cells. Blood 1996;88:1619-31
11 Nishihira H, Kato K, Isoyama K, Takahashi TA, Kai S, Kato S, et al. The Japanese cord blood bank network experience with cord blood transplantation from unrelated donors for haematological malignancies : an evaluation of graft-versus-host disease prophylaxis.Nishihira H, Kato K, Isoyama K, Takahashi TA, Kai S, Kato S, et al. The Japanese cord blood bank network experience with cord blood transplantation from unrelated donors for haematological malignancies : an evaluation of graft-versus-host disease prophylaxis. Br J Haematol 2003; 120:516-222003; 120:516-22   DOI   ScienceOn
12 Thomas SJ, Lamping CP, Ziegler BL. Phenotype analysis of hematopoietic CD34＋ cell populations derived from human umbilical cord blood using flow cytometry and cDNApolymerase chain reaction. Blood 1994;83:2103-14
13 Wagner JE, Kernan NA, Steinbuch M, Broxmeyer HE, Gluckman E. Allogeneic sibling umbilical cord-blood transplantation in children with malignant and non-malignant disease. Lancet 1995;346:214-9   DOI   ScienceOn
14 Li K, Li CK, Chuen CK, Tsang KS, Fok TF, James AE, et al. Preclinical ex vivo expansion of G-CSF-mobilized peripheral blood stem cells : effects of serum-free media, cytokine combinations and chemotherapy. Eur J Haematol 2005;74:128-35   DOI   ScienceOn
15 Gluckman E, Broxmeyer HE, Auerbach AD, Friedman HS, Douglas GW, Devergie A, et al. Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical cord blood from an HLA-identical sibling. N Engl J Med 1989;321:1174-8   DOI   ScienceOn
16 Rocha V, Labopin M, Sanz G, Arcese W, Schwerdtfeger R, Bosi A, et al. Acute Leukemia Working Party of European Blood and Marrow Transplant Group; Eurocord-Netcord Registry. Transplants of umbilical-cord blood or bone marrow from unrelated donors in adults with acute leukemia. N Engl J Med 2004;351:2276-85   DOI   ScienceOn
17 Paquette RL, Dergham ST, Karpf E, Wang HJ, Slamon DJ, Souza L, Glaspy JA. Culture conditions affect the ability of ex vivo expanded peripheral blood progenitor cells to accelerate hematopoietic recovery. Exp Hematol 2002;30:374-80   DOI   ScienceOn
18 Jaroscak J, Goltry K, Smith A, Waters-Pick B, Martin PL, Driscoll TA, et al. Augmentation of umbilical cord blood (UCB) transplantation with ex vivo-expanded UCB cells : results of a phase 1 trial using the AastromReplicell System. Blood 2003;101:5061-7   DOI   ScienceOn
19 Muench MO, Barcena A. Megakaryocyte growth and development factor is a potent growth factor for primitive hematopoietic progenitors in the human fetus. Pediatr Res 2004;55:1050-6.   DOI   ScienceOn
20 Almici C, Carlo-Stella C, Wagner JE, Rizzoli V. Umbilical cord blood as a source of hematopoietic stem cells : from research to clinical application. Haematologica 1995;80:473- 9
21 Cairo MS, Wagner JE. Placental and/or umbilical cord blood : an alternative source of hematopoietic stem cells for transplantation. Blood 1997;90:4665-78
22 Wendling F, Maraskovsky E, Debili N, Florindo C, Teepe M, Titeux M, et al. Humoral regulator of megakaryocytopoiesis. Nature 1994;369:571-4   DOI   ScienceOn
23 Shpall EJ, Quinones R, Giller R. Transplantation of adult and pediatric cancer patients with cord blood progenitors expanded ex vivo [abstract]. Blood 2000;98:851a.   DOI   ScienceOn
24 Seoh JY, Woo SY, Im SA, Kim YJ, Park HY, Lee S, et al. Distinct patterns of apoptosis in association with modulation of CD44 induced by thrombopoietin and granulocytecolony stimulating factor during ex vivo expansion of human cord blood CD34＋ cells. Br J Haematol 1999;107: 176-85   DOI   ScienceOn
25 Laughlin MJ, Barker J, Bambach B, Koc ON, Rizzieri DA, Wagner JE, et al. Hematopoietic engraftment and survival in adult recipients of umbilical-cord blood from unrelated donors. N Engl J Med 2001;344:1815-22   DOI   ScienceOn
26 Kim SK, Koh SK, Song SW, Shin SH, Choi GS, Kim, WC, et al. Ex vivo expansion and clonality of CD34＋ selected cells from bone marrow and cord blood in a serum-free media. Molecules and Cells 2002;14:367-73
27 Rubinstein P, Carrier C, Scaradavou A, Kurtzberg J, Adamson J, Migliaccio AR, et al. Outcomes among 562 recipients of plancental-blood transplants from unrelated donors. N Engl J Med 1998;339:1565-76   DOI   ScienceOn
28 Sanz GF, Saavedra S, Planelles D, Senent L, Cervera J, Barragan E, et al. Standardized, unrelated donor cord blood transplantation in adults with hematologic malignancies. Blood 2001;98:2332-8.   DOI   ScienceOn
29 Terada Y, Hato F, Sakamoto C, Hasegawa T, Suzuki K, Nakamae H, Ohta K, Yamane T, Kitagawa S, Hino M. Thrombopoietin stimulates ex vivo expansion of mature neutrophils in the early stages of differentiation. Ann Hematol 2003;82:671-6   DOI   ScienceOn
30 Schattner M, Lefebvre P, Mingolelli SS, Goolsby CL, Rademaker A, White JG, et al. Thrombopoietin-stimulated ex vivo expansion of human bone marrow megakaryocytes. Stem Cells 1996;14:207-14   DOI   ScienceOn
31 McNiece I, Jones R, Bearman SI, Cagnoni P, Nieto Y, Franklin W, et al. Ex vivo expanded peripheral blood progenitor cells provide rapid neutrophil recovery after highdose chemotherapy in patients with breast cancer. Blood 2000;96:3001-7
32 Lefebvre P, Winter JN, Kahn LE, Giri JG, Cohen I. Megakaryocyte ex vivo expansion potential of three hematopoietic sources in serum and serum-free medium. J Hematotherapy 1999;8:199-208   DOI   ScienceOn
33 Lyman SD. Biology of flt3 ligand and receptor. Int J Hematol 1995;62:63-73   DOI   PUBMED   ScienceOn
34 Bertolini F, Battaglia M, Pedrazzoli P, Da Prada GA, Lanza A, Soligo D, et al. Megakaryocytic progenitors can be generated ex vivo and safely administered to autologous peripheral progenitor cell transplant recipients. Blood 1997; 89:2679-88