• Journal of the Institute of Convergence Signal Processing
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• v.16 no.2
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• pp.74-82
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• 2015

In this study, a BMW plant process control system model which produces BMW is suggested and the BMW plant process controller with the following functions is developed. The first function is to operate the electronic overload relays to stop the blower for a certain period of time and to re-operate it again when the blower is overloaded. The second function is to close the motor operated valve automatically in case of power failure to prevent the circulation from the guided tank to the compost throwing tank and to block leak from the compost throwing tank due to the failure of ball valve. The third function is to transfer produced BMW from the concentration tank to 4 storage tanks for automatic managing of the BMW output. A device to measure the signal of the BMW plant process controller and a test equipment are developed. The designed BMW plant process controller is checked to see if it operates correctly according to the design specifications. The sequence control method based on BMW plant process controller is developed at a low cost in this study, so it is expected to bring improvements in the stability and the efficiency of system and to cause reductions in the operation and the management costs in the future.

• Korean Journal of Ichthyology
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• v.32 no.2
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• pp.39-48
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• 2020

The slender shinner (Pseudopungtungia tenuicorpa), a tiny freshwater fish of about 8 to 10 cm belonging to Cyprinidae, is an endangered species found only in the Han and Imjin Rivers on the Korean Peninsula. During the breeding season, this species spawns in nests of Coreoperca herzi, a predator of this species, or small crevices on rocks. This unique reproductive ecology can make this species more vulnerable to anthropogenic perturbance that can further limit the places to spawn. Here, mtDNA and microsatellite loci were analyzed to identify the genetic diversity and structure of slender shinners and further to provide the basic data necessary for the conservation planning of this species. A total of 28 polymorphic microsatellite markers were developed using Illumina paired-end sequencing, and 67 slender shinners collected from three localities in the Han River were genotyped using these loci. This species showed a remarkably high level of genetic diversity with mean expected heterozygosity of 0.914 and mean allele number per locus of 27.9, and no signature of drastic demographic decline was detected. As a result of our microsatellite analysis, the genetic structure between the two stems of the Han River, North Han and South Han, was prominent. Such a genetic structure was also evident in the sequence analysis of 14 haplotypes obtained from mtDNA control region. Although slender shinners are only found in very limited areas around the world, the genetic structure indicates that there is a block of gene flow among the populations, which should be reviewed in the future if management and restoration of this species is needed.

• Environmental Analysis Health and Toxicology
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• v.31
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• pp.10.1-10.8
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• 2016

Objectives Aromatase inhibitors that block estrogen synthesis are a proven first-line hormonal therapy for postmenopausal breast cancer. Although it is known that standardized extract of Ginkgo biloba (EGb761) induces anti-carcinogenic effects like the aromatase inhibitors, the effects of EGb761 on steroidogenesis have not been studied yet. Therefore, the effects of EGb761 on steroidogenesis and aromatase activity was studied using a H295R cell model, which was a good in vitro model to predict effects on human adrenal steroidogenesis. Methods Cortisol, aldosterone, testosterone, and $17{\beta}$-estradiol were evaluated in the H295R cells by competitive enzyme-linked immunospecific assay after exposure to EGb761. Real-time polymerase chain reaction were performed to evaluate effects on critical genes in steroid hormone production, specifically cytochrome P450 (CYP11/ 17/19/21) and the hydroxysteroid dehydrogenases ($3{\beta}$-HSD2 and $17{\beta}$-HSD1/4). Finally, aromatase activities were measured with a tritiated water-release assay and by western blotting analysis. Results H295R cells exposed to EGb761 (10 and $100{\mu}g/mL$) showed a significant decrease in $17{\beta}$-estradiol and testosterone, but no change in aldosterone or cortisol. Genes (CYP19 and $17{\beta}$-HSD1) related to the estrogen steroidogenesis were significantly decreased by EGb761. EGb761 treatment of H295R cells resulted in a significant decrease of aromatase activity as measured by the direct and indirect assays. The coding sequence/Exon PII of CYP19 gene transcript and protein level of CYP19 were significantly decreased by EGb761. Conclusions These results suggest that EGb761 could regulate steroidogenesis-related genes such as CYP19 and $17{\beta}$-HSD1, and lead to a decrease in $17{\beta}$-estradiol and testosterone. The present study provides good information on potential therapeutic effects of EGb761 on estrogen dependent breast cancer.

• BMB Reports
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• v.30 no.5
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• pp.303-307
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• 1997

Grb2, which is composed of a Src homology 2 (SH2) domain and two Src homology 3 (SH3) domains, is known to serve as an adaptor protein in signaling for Ras activation. Thus, a blocker of the Grb2 interactions with other proteins can be a potential candidate for an anticancer drug. In this study, we have developed a high throughput screening method for SH3 domain binding ligands and blockers. Firstly, we made and purified the glutathione S-transferase (GST)-fusion proteins with the Grb2 SH2 and SH3 domains, and the entire Grb2. This method measures the binding of a biotin-labeled oligopeptide, derived from a Grb2/SH3 binding motif in the hSos, to the GST-fusion proteins, which are precoated as glutathione S-transferase fusion protein on a solid phase. When $1\;{\mu}g$ of each fusion protein was used to coat the wells, both N- and C- terminal SH3 the domains as well as the whole of Grb2 were able to interact with the biotin-conjugated ligand peptide, while the SH2 domain and GST alone showed no binding affinity. Although N- and C- terminal SH3 domains showed an increase of binding to the ligand peptide in proportion to the amount of peptide, the GST fusion protein with Grb2 demonstrated much higher binding affinity. GST-Grb2 coating on the solid phase showed a saturation curve; 66 and 84% of the maximal binding was observed at 100 and 300 ng/$100\;{\mu}l$, respectively. This binding assay system was peptide sequence-specific, showing a dose-dependent inhibition with the unlabeled peptide of SH3 binding motif. Several other peptides, such as SH2 domain binding motifs and PTB domain binding motif, were ineffective to inhibit the binding to the biotin-conjugated ligand peptide. These results suggest that our method may be useful to screen for new anticancer drug candidates which can block the signaling pathways mediated by SH3 domain binding.

• Research in Plant Disease
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• v.21 no.4
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• pp.315-320
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• 2015

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at $58^{\circ}C$ for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.3 s.309
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• pp.9-16
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• 2006

Many image and video compression algorithms work by splitting the image into blocks and producing variable-length code bits for each block data. If variable-length code data are transmitted consecutively over error-prone channel without any error protection technique, the receiving decoder cannot decode the stream properly. So the standard image and video compression algorithms insert some redundant information into the stream to provide some protection against channel errors. One of redundancies is resynchronization marker, which enables the decoder to restart the decoding process from a known state in the event of transmission errors, but its usage should be restricted not to consume bandwidth too much. The Error Resilient Entropy Code(EREC) is well blown method which can regain synchronization without any redundant information. It can work with the overall prefix codes, which many image compression methods use. This paper proposes EREREC method to improve FEREC(Fast Error-Resilient Entropy Coding). It first calculates initial searching position according to bit lengths of consecutive blocks. Second, initial offset is decided using statistical distribution of long and short blocks, and initial offset can be adjusted to insure all offset sequence values can be used. The proposed EREREC algorithm can speed up the construction of FEREC slots, and can improve the compressed image quality in the event of transmission errors. The simulation result shows that the quality of transmitted image is enhanced about $0.3{\sim}3.5dB$ compared with the existing FEREC when random channel error happens.

• Korean Journal of Construction Engineering and Management
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• v.15 no.6
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• pp.26-34
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• 2014

Highway construction is a combination of linear, repetitive, and highly equipment intensive operations. Various types of construction equipment are deployed to ensure undisrupted performance of construction, and thus productivity improvement and cost-saving can be achieved through well-thought-out planning. The selection of construction equipment is dependent upon construction sequence, site conditions, and construction methods. In the process of planning, management should consider various types of construction methods per each type of construction operation. Also, management should map out proper construction equipment operation plan that takes the construction duration and cost measures into consideration. However, limited availability of historic data from the similar types of operations has been a stumbling block to proper construction planning, making the operations performed based upon experience and intuition guided by rules-of-thumb. As a consequence, the planing phase rarely provided an adequate validity in the implementation phase. The researchers developed a process logic for each construction type that management can utilize from early phase of highway construction planning process. Moreover, derived the construction equipment combination optimized for efficiency by using the process simulation technique. The developed method is expected to be useful for the decision-making process that aims to evaluate efficiency of various process plans and to ensure optimal selection of construction equipment for highway construction projects.

• Journal of the Korean Geophysical Society
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• v.3 no.1
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• pp.1-12
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• 2000

A palaeomagnetic study was carried out on Early through Late Cretaceous sandstones and volcanic sequences (the Songnaedong Formation, Chaeyaksan Volcanics, Konchonri Formation, and Jusasan Andesite it ascending order) from Konchonri area in the northern Milyang subbasin of the Kyongsang Basin, Korea. A high-temperature stable remanence with direction of $d=22.9^{\circ},\;i=59.1^{\circ}\;({\alpha}_{95}=3.0^{\circ})$ has been isolated and a corresponding pole was $71.6^{\circ}N,\;199.6^{\circ}E\;(A_{95}=4.2^{\circ})$. The characteristic high-temperature component resides in both hematite and magnetite. The primary nature of this remanence is confirmed from positive fold and reversals tests, The palaeopole is consistent with those of the Hayang Group in other parts of the Kyongsang Basin. A comparison of the palaeomagnetic pole position from the studied area with the contemporary pole from China west of the Tan-Lu fault presents that Konchonri area has experienced little latitudinal displacement nor vertical-axis block rotation relative to the Chinese blocks since the Cretaceous. Based on the formations indicating dual polarity, radiometric and paleontologic data, the magnetostratigraphic age of the studied sequence from the Songnedong Formation to the Jusasan Andesite ranges from upper Albian to lower Campanian reverse polarity chronozone. On the other hand, volcanic samples of the Chaeyaksan Volcanics and the Jusasan Andesite showed the scattered directions considered in group, even though individual sample showed a stable remanent magnetization in response to thermal demagnetization. It indicates that they have been reworked after acquisition of the stable remanent magnetization.

• Journal of Broadcast Engineering
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• v.7 no.1
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• pp.45-54
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• 2002

This paper proposes a video compression algorithm using characteristics of wavelet coefficients. The proposed algorithm can provide lowed bit rate and faster running time while guaranteeing the reconstructed image qualify by the human virtual system. In this approach, each video sequence is decomposed into a pyramid structure of subimages with various resolution to use multiresolution capability of discrete wavelet transform. Then similarities between two neighboring frames are obtained from a low-frequency subband which Includes an important information of an image and motion informations are extracted from the similarity criteria. Four legion selection filters are designed according to the similarity criteria and compression processes are carried out by encoding the coefficients In preservation legions and replacement regions of high-frequency subbands. Region selection filters classify the high-frequency subbands Into preservation regions and replacement regions based on the similarity criteria and the coefficients In replacement regions are replaced by that of a reference frame or reduced to zero according to block-based similarities between a reference frame and successive frames. Encoding is carried out by quantizing and arithmetic encoding the wavelet coefficients in preservation regions and replacement regions separately. A reference frame is updated at the bottom point If the curve of similarity rates looks like concave pattern. Simulation results show that the proposed algorithm provides high compression ratio with proper Image quality. It also outperforms the previous Milton's algorithm in an Image quality, compression ratio and running time, leading to compression ratio less than 0.2bpp. PSNR of 32 dB and running tome of 10ms for a standard video image of size 352${\times}$240 pixels.

• Economic and Environmental Geology
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• v.25 no.1
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• pp.61-72
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• 1992

The Danyang area consists of the thrust and folded sedimentary rocks of Paleozoic and Mesozoic Era. The area is bounded by major tectonic units which are the Gagdong Thrust to the west and the Okdong Fault to the east. According to the structural analyses, the area is affected by polyphase deformation. This study establishes deformational sequence in the area. Mylonite zone along the Okdong Fault corresponds to the first generation of structures ($D_1$). $D_1$-structures are discrete shear zone in the Jangsan Formation and bedding parallel extensional deformation in the Cambro-Ordovician sequences. $D_2$-structures were formed prior to the sedimentation of the Jurassic Bansong Group, which are the NW-trending fold and linear structures. After sedimentation of the Bansong Group, the area is strongly affected by the Daebo Orogeny which produces NE-trending thrusts, folds and linear structures. Earlier structures were tightened and rotated toward NE. Some thrust faults did not propagate into the Bansong Group. It is suggested either the Bansong Group acted as a decoupling horizon or rest on unconformably on the thrust faults. The area is weakly affected by $D_4$-event of which structures are E-W trending folds and faults. The Jugryeong Fault clearly cut the earlier folds and thrust faults. The rocks within the fault zone were sliced and rotated during the strike-slip movements. Block rotation and transpressional features can be commonly observed.