• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.341-346
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• 1998

In this paper we describe the cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme dihydroflavonol 4-reductase (DFR) in Matthiola incana R. Br. A heterologous cDNA probe from Zea mays was used to isolate full-size DFR cDNA clone from a corolla-specific cDNA library. Comparison of the coding region of this DFR cDNA sequence including the sequences of Zea mays, Anthirrinum majus, Petunia hybrida, Callistephus chinensis, Dianthus caryophyllus and Rosa hybrida reveals a identity higher than 61% at the nucleotide level. The DFR transcript is G/C rich in monocotyledonous plants show a strong codon bias preferring codons with a G or C in the third position. The function of this nucleotide sequences were verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRNA from wild type and mutant plants and by in vitro expression yielding an enzymatically active reductase. Genomic southern blot analysis showed the presence of one gene for DFR in Matthiola incana. Northern blot analysis of the DFR wild type and mutant lines showed that the lack of DFR activity in the stable acyanic mutant k17b is clearly by a transcriptional block of the DFR gene.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.10
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• pp.979-985
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• 2010

This study was performed to detect enterococci strain as an indicator of faecal contamination, to identify of 16S rDNA sequence and vancomycin resistance by MIC (Minimum Inhibitory Concentration) test from drinking spring-water samples in Seoul. The detection frequency of enterococci was 42 (19.8%) among 212 samples, and its concentration was ranged from 0 to 110 CFU/100 mL. These results were confirmed the possibility as an indicator microorganisms that similar to the frequency of E. coli detection (t test p-value 0.268, significant level 0.05). Isolated 56 enterococci samples were identified by 16S rDNA sequence data and their NCBI BLAST searching. They were identified to Enterococcus faecalis of 24 samples, E. faecium (10), E. casseliflavus (10), E. gallinarum (3), E. hirae (2), E. durans (2), E. sanguinicola (1). E. faecalis was dominant species that clinical case report of a domestic was similar. Vancomycin resistant enterococci (VRE) of 53 samples showed that vanB and vanC1/C2 type with 2 and 12 case, respectively. These results indicated that the drinking spring-water quarantined to fecal pollution for block out outbreak of gastrointestinal symptom with using such as disinfection process.

• The Korean Journal of Petroleum Geology
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• v.6 no.1_2 s.7
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• pp.20-24
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• 1998

Expert system is one type of artificial intelligence softwares that incorporate problem-solving knowledges and experiences of human experts by use of symbolic reasoning and rules about a specific topic. In this study, an expert system, PLAYMAKER2, is used to interpret sedimentary facies and depositional settings of the sedimentary sequence. The original version of the expert system, PLAYMAKER, was developed in University of South Carolina in 1990, and modified into the present PLAYMAKER2 with some changes in the knowledge-base of the previous system. The late Cenozoic sedimentary sequence with maximum 10,000 m in thickness, which is located in the Korean Oil Exploration Block VI-1 at the southwestern margin of the Ulleung Basin, is analysed by the expert system, PLAYMAKER2. The Cenozoic sedimentary sequence is divided into two units-lower Miocene and upper Pliocene-Pleistocene sediments. The depositional settings and sedimentary facies of the Miocene sediments interpreted by PLAYMAKER2 in terms of belief values are: for depositional settings, slope; $57.4\%$, shelf; $21.4\%$, basin; $10.1\%$, and for sedimentary facies, submarine fan; $35.7\%$, continental slope; $26.3\%$, delta; $16.1\%$, deep basinplain; $6.1\%$ continental shelf; $3.2\%$, shelf margin; $1.4\%$. The depositional settings and sedimentary facies of the Pliocene-Pleistocene sediments in terms of belief values we: for depositional settings, slope; $59.0\%$, shelf; $22.8\%$, basin; $7.0\%$, and for sedimentary facies, delta; $24.1\%$, continental slope; $22.2\%$, submarine fan; $17.3\%$, continental shelf; $7.0\%$, deep basinplain; $4.8\%$, shelf margin; $2.6\%$. The comparison of the depositional settings and sedimentary facies consulted by PLAYMAKER2 with those of the classical interpretation from previous studies shows resonable similarity for the both sedimentary units-the lower Miocene sediments and the upper Pliocene-Pleistocene sediments. It demonstrates that PLAYMAKER2 is an efficient tool to interpret the depositional setting and sedimentary facies for sediments. However, to be a more reliable system, many sedimentologists should work to refine and add geological rules in the knowledge-base of the expert system, PLAYMAKER2.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.91-94
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• 2014

In June 2013, surface rot symptoms were observed on sweet potatoes (Ipomoea batatas) in Yeoju city, Korea. The lesions were circular, light to dark brown, firm, dry, and superficial. The diseased area became sunken in older tissues, and the symptoms usually did not extend deeply into the flesh. Seven isolates of Fusarium species were isolated from diseased sweet potatoes. All isolates were identified as Fusarium oxysporum based on morphological characteristics on CLA medium. To confirm the identification, molecular analysis of elongation factor 1 alpha gene was conducted. Among the isolates, however, four isolates were F. commune, and three isolates were F. oxysporum based on the DNA sequence data. Pathogenicity was tested using agar block inoculation on wounded or unwounded sweet potato pieces. Lesions were observed on wounded sweet potato pieces after 7 days inoculation in only F. commune isolates. This is the first report that F. commune causes Fusarium surface rot of sweet potato in Korea.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.9 no.1
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• pp.75-87
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• 2009

We propose in this paper a novel approach to image fusion in which the fusion rule is guided by optimizing an image clarity function. A Genetic Algorithm is used to stochastically select, comparative to the clarity function, the optimum block from among the source images. A novel nested Genetic Algorithm with gifted individuals found through bombardment of genes by the mutation operator is designed and implemented. Convergence of the algorithm is analytically and empirically examined and statistically compared (MANOVA) with the canonical GA using 3 test functions commonly used in the GA literature. The resulting GA is invariant to parameters and population size, and a minimal size of 20 individuals is found to be sufficient in the tests. In the fusion application, each individual in the population is a finite sequence of discrete values that represent input blocks. Performance of the proposed technique applied to image fusion experiments, is characterized in terms of Mutual Information (MI) as the output quality measure. The method is tested with C=2 input images. The results of the proposed scheme indicate a practical and attractive alternative to current multi-focus image fusion techniques.

• Journal of Korea Multimedia Society
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• v.12 no.7
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• pp.922-928
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• 2009

In H.264 as the newest video standard, 9 modes are used in order to predict the signal values of a block composed with several pixels by intra prediction. From these process, H.264 can bring high compression ratio in the encoded signal but the use of total 9 modes can give the inefficiency of the increase of the complexity induced by the amount of operation processing or the number of searching which is applied to compare adjacent pixels. This paper proposes a simplification method of prediction mode for the intra-picture coding by considering subjective interest region. There are certain region being interested within a picture of the video sequence. This region requires better subjective picture quality than the other regions. The proposed method increases the simplification of prediction mode by providing just essential modes of total 9 modes for less interest regions compared with the interest region. It is possible to get the additional 11%$\sim$15% simplification of the prediction mode by the proposed method, compared with the conventional method which simplifies the prediction mode for all of the picture by using the prediction characteristics only.

• BMB Reports
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• v.40 no.4
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• pp.571-576
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• 2007

Three anti-apoptosis genes, Ls-iap2, iap3 and p49 were found in Leucania separata multiple nuclear polyhedrovirus. Amino acid sequence homology of Ls-IAP2 and Ls-IAP3 with Op-IAP2 and Op-IAP3 from Orgyia pseddotsugata MNPV were 20% and 42%, while that of Ls-P49 is 28% with Sl-P49 from Spodoptera littorolis MNPV. Ls-IAP2 contains one baculoviral IAP repeat (BIR) domain followed by a RING domain, while Ls-IAP3 contains two BIRs and a RING. Ls-P49 contains a reactive site loop, predicted cleavage site (KKLD$^{74}{\downarrow}$G) that is different from Sl-P49 (TVID$^{94}{\downarrow}$G). Expressed Ls-iap3 or Ls-p49 under presence of actinomycin D in SF9 cells, DNA ladder assayrevealed that Ls- IAP3 or Ls-P49 could block the apoptosis of SF9 cells induced by actinomycin D. Replication of p35 deficient-mutant Autographa californica MNPV in SF9 cells was also rescued when Ls-iap3 or Ls-p49 was expressed transiently. No anti-apoptotic activity was observed for Ls-IAP2. The results showed that both of Ls-IAP3 and Ls-P49 were functional apoptotic suppressors in SF9 cells.

• Archives of Plastic Surgery
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• v.37 no.5
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• pp.644-649
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• 2010

Purpose: As the mean life expectancy of people has been prolonged, and the elderly people who participate in the production activities has been increasing, it is expected that the demand on the replantation of amputated digits in elderly patients would increase. But, there are few studies about the replantation of amputated digits in elderly patients. Therefore, we report treatment outcomes of replantation of amputated digits in elderly patients. Methods: From 1998 to 2008, the replantation was performed in 51 completely amputated digits of 33 patients aged 60 years or older. We performed the replantation in the usual manner. Under the brachial plexus block, the surgical procedures carried out in the following sequence: internal fixation using Kirschner wire, tenorrhaphy, arteriorrhaphy, neurorrhaphy and venorrhaphy. If the arterial ends could not be approximated without tension, a vein graft was performed. Results: Of a total of 51 digits, 46 digits (90%) survived. 13 patients (40%) had underlying medical problem preoperatively. But, in all the patients, there were no postoperative medical complications. As the postoperative surgical complications, excluding five cases of the total necrosis of digit, there were three cases of venous congestion, two cases of arterial insufficiency, seven cases of infection and 16 cases of partial necrosis. Conclusion: Age alone does not affect the survival of replanted digits. Type of injury is the most important factor that affects the survival of replanted digits.

• The KIPS Transactions:PartB
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• v.17B no.6
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• pp.405-412
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• 2010

Previous background generation techniques showed bad performance in complex environments since they used only temporal contexts. To overcome this problem, in this paper, we propose a new background generation method which incorporates spatial as well as temporal contexts of the image. This enabled us to obtain 'clean' background image with no moving objects. In our proposed method, first we divided the sampled frame into m*n blocks in the video sequence and classified each block as either static or non-static. For blocks which are classified as non-static, we used MRF framework to model them in temporal and spatial contexts. MRF framework provides a convenient and consistent way of modeling context-dependent entities such as image pixels and correlated features. Experimental results show that our proposed method is more efficient than the traditional one.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.5
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• pp.438-443
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• 2004

Objectives : The proper development of the facial structures relies upon a sequence of tightly regulated signaling interactions between the ectoderm and mesoderm involving the participation of several families of signaling molecules. Among these, bone morphogenetic proteins (BMPs) have been suggested to be a key signal that regulates the development of the mandible and the initiation and morphogenesis of the teeth. The aim of this study was to examine the artificial development of the mandibular structures and to examine the role of BMPs on tooth morphogenesis and differentiation using an organ culture system. Materials and Methods : The tooth germs from Ed 11.5, 13.5 mice were dissected, and transplanted into the diastema of the mandible primordia. The mandibles containing the transplanted tooth germs were cultured in vitro. During this period, beads soaked with BMP4 were implanted around the transplanted tooth germs. In addition, a diastema block containing the transplanted tooth germ was dissected, then transferred to an adult mouse kidney. After the organ culture, the developing mandibular explant was removed from the kidney and prepared for the tissue specimens. Odontogeneis of the transplanted tooth germs was examined after Hematoxylin-eosin, Masson-trichrome staining. Results : Proliferation and differentiation of the tooth germs cultured in the diastema was observed. In the BMP4-treated tooth germs, the formation of the first and second molars was noted. The crown of the developing tooth showed the formation of a mature cusp with the deposition of enamel and dentin matrix. In conclusion, it was confirmed that BMP4 is involved in the formation of a dental crown and the differentiation of ameloblasts and odontoblasts of the molar tooth during the development of the transplanted tooth germs.