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Detection of Enterococci and their Vancomycin Resistance in Drinking Spring-Water  

Yoon, Tae-Ho (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Hyang (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Lee, Seung-Joo (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Yeo, In-Hak (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Eom, Seok-Won (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Publication Information
Journal of Korean Society of Environmental Engineers / v.32, no.10, 2010 , pp. 979-985 More about this Journal
Abstract
This study was performed to detect enterococci strain as an indicator of faecal contamination, to identify of 16S rDNA sequence and vancomycin resistance by MIC (Minimum Inhibitory Concentration) test from drinking spring-water samples in Seoul. The detection frequency of enterococci was 42 (19.8%) among 212 samples, and its concentration was ranged from 0 to 110 CFU/100 mL. These results were confirmed the possibility as an indicator microorganisms that similar to the frequency of E. coli detection (t test p-value 0.268, significant level 0.05). Isolated 56 enterococci samples were identified by 16S rDNA sequence data and their NCBI BLAST searching. They were identified to Enterococcus faecalis of 24 samples, E. faecium (10), E. casseliflavus (10), E. gallinarum (3), E. hirae (2), E. durans (2), E. sanguinicola (1). E. faecalis was dominant species that clinical case report of a domestic was similar. Vancomycin resistant enterococci (VRE) of 53 samples showed that vanB and vanC1/C2 type with 2 and 12 case, respectively. These results indicated that the drinking spring-water quarantined to fecal pollution for block out outbreak of gastrointestinal symptom with using such as disinfection process.
Keywords
Enterococci; Drinking Spring-water; VRE;
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