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Occurrence of Fusarium Surface Rot on Sweet Potato Caused by Fusarium commune

Fusarium commune에 의한 고구마 표피썩음병의 발생

  • 최효원 (농촌진흥청 국립농업과학원) ;
  • 홍성기 (농촌진흥청 국립농업과학원) ;
  • 이영기 (농촌진흥청 국립농업과학원) ;
  • 남영주 (농촌진흥청 국립농업과학원) ;
  • 이재금 (농촌진흥청 국립농업과학원) ;
  • 안치중 (여주시 농업기술센터)
  • Received : 2014.03.17
  • Accepted : 2014.03.26
  • Published : 2014.03.31

Abstract

In June 2013, surface rot symptoms were observed on sweet potatoes (Ipomoea batatas) in Yeoju city, Korea. The lesions were circular, light to dark brown, firm, dry, and superficial. The diseased area became sunken in older tissues, and the symptoms usually did not extend deeply into the flesh. Seven isolates of Fusarium species were isolated from diseased sweet potatoes. All isolates were identified as Fusarium oxysporum based on morphological characteristics on CLA medium. To confirm the identification, molecular analysis of elongation factor 1 alpha gene was conducted. Among the isolates, however, four isolates were F. commune, and three isolates were F. oxysporum based on the DNA sequence data. Pathogenicity was tested using agar block inoculation on wounded or unwounded sweet potato pieces. Lesions were observed on wounded sweet potato pieces after 7 days inoculation in only F. commune isolates. This is the first report that F. commune causes Fusarium surface rot of sweet potato in Korea.

2013년 6월, 경기도 여주시에서 저장 중인 고구마 괴근 표면에 갈색의 썩음 증상이 관찰되었다. 병징은 둥글고, 옅은 갈색 내지 진한 갈색이며, 단단하고 건조한 편으로 표피에 한하여 얄팍하게 나타났다. 병반 부위는 오래된 부위에서 움푹 패이고, 괴근의 내부로 깊게 썩히지 않았다. 병든 고구마에서 7개의 Fusarium균을 분리하였고, CLA 배지에서 균학적 특징을 조사한 결과 F. oxysporum으로 동정되었다. 이러한 결과를 확인하기 위하여 elongation factor 1 ${\alpha}$ 유전자 부위의 염기서열을 분석하였다. 그 결과, 7개 균주 중 4개는 F. commune으로, 3개는 F. oxysporum으로 확인되었다. 고구마 괴근을 상처구와 무상처구로 구분하고, 균사절편을 접종하여 병원성을 확인한 결과, 접종 7일 후 F. commune을 접종한 상처구에서만 병징이 관찰되었다. 따라서 본 병을 Fusarium commune에 의한 고구마 표피썩음병으로 명명하며, 국내에서 최초로 보고한다.

Keywords

References

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