• Journal of Microbiology
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• v.45 no.5
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• pp.402-408
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• 2007

A strain producing a potent protease was isolated from turban shell. The strain was identified as Bacillus sp. S17110 based on phylogenetic analysis. The enzyme was purified from culture supernatant of Bacillus sp. S17110 to homogeneity by ammonium sulfate precipitation, SP-Sepharose, and DEAE-Sepharose anion exchange chromatography. Protease activity of the purified protein against casein was found to be stable at pH 7 to pH 10 and around $50^{\circ}C$. Approximately 70% of proteolytic activity of the enzyme was detected either in the presence of 100 mM SDS or Tween 20. The enzyme activity was enhanced in the presence of $Ca^{2+},\;Zn^{2+},\;Mg^{2+}$, but was inhibited by EDTA, indicating that it requires metal for its activity. The purified enzyme was found to be a monomeric protein with a molecular mass of 75 kDa, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography. The purified enzyme was analyzed through peptide fingerprint mass spectra generated from matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) and a BLAST search, and identified as immune inhibitor A (inhA) deduced from nucleotide sequence of B. cereus G9241. Since InhA was identified as protease that cleave antibacterial proteins found in insect, inhA-like protease purified from Bacillus sp. S17110 might be pathogenic to sea invertebrates.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1993-2005
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• 2016

Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain's extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome. The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed for B. pumilus. These data provide B. pumilus a comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.

• Journal of Ginseng Research
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• v.34 no.1
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• pp.17-22
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• 2010

Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.

• IMMUNE NETWORK
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• v.2 no.1
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• pp.35-40
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• 2002

Background: CTLA-4 (Cytotoxic T Lymphocyte associated Antigen 4, CD152) has been known as a homologue of CD28, an accessory molecule providing a key costimulatory signal for successful antigen-driven activations of T lymphocyte. Most of biochemical and cell biological characteristics of the CD152 protein remain unknown while those of CD28 have been characterized in detail. Methods: In this study CD152 expression in both $CD4^+$ and $CD8^+$ PBLs was studied by using flow cytometry. And intracellular CD152 multiprotein complex was purified and used for generating antibodies recognizing proteins composing of intracellular CTLA-4 multi protein complex. Results: Level of surface expression of this molecule was peaked at 2 days of PHA stimulation in flow cytometric analysis. 40~45% of PHA blast cells were $CD152^+$ in both of two subsets at this stage and the level of expression were equivalent in both two subsets. Contrary to this surface expression, intracellular expression was peaked at day 3 and it was preferentially induced in $CD8^+$ cells and about 60% of $CD8^+$ cells were $CD152^+$ at this stage. High molecular weight (>350 kD) intacellular CD152 protein complex purified by using preparative electrophoresis were immunized into rabbits and then 3 different anti-P34PC4, anti-P34PC7 and anti-P34PC8 antibodies were obtained. Using these 3 antibodies two unknown antigens associated with intracellular CD152 multiprotein complex were found and their molecular weights were 54 kD and 75 kD, respectively. Among these, the former was present as 110 kD homodimer in non-reducing condition. Conclusion: It seemed that 34 kD intracellular CD152 molecule forms high molecular weight multiprotein complex at least with 2 proteins of 75 kD monomer and 110 kD homodimer.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.9 s.339
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• pp.41-48
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• 2005

In this paper, we suggest the scheme combining long-term feedback and short-term feedback in MIMO multi-user system and analyze its system performance. At the beginning of communication, long-term feedback is fed back to transmitter such as the number of active antennas, constellations, and transmission powers which are determined to satisfy the given target symbol error and target data rate for each user. After this, short-term feedback of active antenna index is fed back periodically Simulation results show the proposed scheme outperforms better than long-term feedback or short-term feedback information scheme.

• Journal of the Korea Concrete Institute
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• v.23 no.1
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• pp.23-30
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• 2011

In this study, deterioration degrees of concrete were investigated at laboratory under seawater attack and cycling freeze-thaw, which are major durability performance deterioration factors of concrete. Deteriorations of mixed concrete using Portland & blended cement were examined by instrumental analysis of changes in relative dynamic modulus of elasticity and compressive strength. After 520 cycles of freeze-thaw, relative dynamic modulus of elasticity and compressive strength of concrete mixed with normal Portland and LHC over 75% showed relatively low resistance of approximately 44% of those values of SRC. Concrete replaced with 50% fine powder of blast furnace slag showed the most excellent freeze-thaw resistance among the tested blended cement concrete.

• The Journal of Advanced Prosthodontics
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• v.1 no.2
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• pp.75-84
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• 2009

STATEMENT OF PROBLEM. A number of studies about the nano-treated surfaces of implants have been conducting along with micro-treated surfaces of implants. PURPOSE. The purpose of this study was to get information for the clinical use of nano-treated surfaces compared with micro-treated surfaces by measuring removal torque and analyzing histological characteristics after the placement of various surface-treated implants on femurs of dogs. MATERIAL AND METHODS. Machined surface implants were used as a control group. 4 nano-treated surface implants and 3 micro-treated surface implants [resorbable blast media surface (RBM), sandblast and acid-etched surface (SAE), anodized RBM surface] were used as experimental groups. Removal torque values of implants were measured respectively and the histological analyses were conducted on both 4weeks and 8weeks after implant surgery. The surfaces of removed implants after measuring removal torque values were observed by scanning electron microscopy (SEM) at 8 weeks. RESULTS. 1. Removal torque values of the nano-treated groups were lower than those of micro-treated groups. 2. Removal torque values were similar in the anodized RBM surface groups. 3. On the histological views, there was much of bone formation at 8 weeks, but there was no difference between 4 and 8 weeks, and between the types of implant surfaces as well. CONCLUSION. it is suggested that implant topography is more effective in removal torque test than surface chemistry. To get better clinical result, further studies should be fulfilled on the combined effect of surface topography and chemistry for the implant surface treatments.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2006.11a
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• pp.5-8
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• 2006

This study investigates the field application, Songdo the # 1st WORLD, on zero-crack construction of the fundamental mass concrete using double bubble sheets and applying low heat mixture. Experimental results of hydration heat analysis showed that crack modulus of concrete incorporating 20% of blast furnace slag cement was 1.0 in 120 hours, representing 50% probability of crack occurrence, thus requiring additional measures. As for a curing method, a specimen insulating two layers of vinyl chloride+double bubble sheets exhibited only $16.5^{\circ}C$ difference between upper and lower sections, and it also showed favorable workability as well as competitive economic side. Therefore it was determined to use it for curing method in this field. For the curing results of practical field, using 2 layers of vinyl chloride+double bubble sheets and applying low heat mixture on the fundamental mass concrete in 3A residential building exhibited less than only $15^{\circ}C$ difference between surface and center section of that in 5 days elapse and less than $20^{\circ}C$ in 9 days. This means that the crack by hydration heat was prevented, and other fields structures also resisted the plastic shrinkage by insulating the vinyl, sinking crack by second temping, even drying shrinkage by the mixture of low unit water. Therefore the crack on the placement has not been found so far, since the construction was started before 6 month.

• Journal of Korean Tunnelling and Underground Space Association
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• v.16 no.4
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• pp.373-386
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• 2014

This paper concerns the deformation behavior of tunnels crossing weak zone during excavation. A three dimensional finite element model was adopted in order to conduct a parametric study on the orientation of weaj zone in terms of strike and dip angle relative to the tunnel longitudinal axis. The results of the analyses were then analyzed so that the tunnel displacements in terms of the ratios between the crown settlement and springline displacement can be related to the orientation of the weak zone. The results indicate that the displacement ratios between the tunnel crown and springline tend to quantitatively change when a weak zone exists near or ahead of the tunnel suggesting that the displacement ratios can be effectively used to predict the weak zone during tunnelling. Practical implications of the findings are discussed.

• Journal of the Society of Naval Architects of Korea
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• v.35 no.1
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• pp.72-79
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• 1998

Static deformation theory of elastoplastic structures can be applied only if the magnitude of loading is less than the plastic collapse force $F_c$. However, with impact or explosive blast loading, the structure can be subjected to an intense but short-duration force pulse that exceeds the plastic collapse force and initiates structural collapse. In this paper, the dynamic response of a rigid-perfectly plastic cantilever subject to intense impact loading is examined in terms of the plastic collapse force. When a step loading is applied, the motion of the beam is calculated and analyzed through the non-dimensionalization of variables. It is concluded that the motion of a beam can be characterized as a function of the nondimensionalized force parameter, $f{\equiv}F/F_c$, where $F_c$ represents the critical force for plastic collapse. This result is used to the analysis of the beam motion subject to rectangular force pulse.