• International Journal of Industrial Entomology and Biomaterials
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• v.44 no.2
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• pp.44-54
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• 2022

The food source of white-spotted flower chafer, Protaetia brevitarsis is important to economical performance. The P. brevitarsis larva were feed by mulberry and oak fermented sawdust. The effect of additional additives, Korean black raspberry (called bokbunja) marc and blueberry marc to fermented sawdust feed on the development and oviposition characteristics were investigated. The development periods of P. brevitarsis was decreased from 165.7 d to 40.0 d (mulberry sawdust) and 169.3 d to 39.0 d (oak sawdust), but the survival rate of larva was not affected by the addition of Korean black raspberry marc to fermented sawdust. However, blueberry marc addition to the fermented sawdust did not significant effect on the development period and survival rate of P. brevitarsis. The percentage of larva weight over 2.5 g was tend to increase by addition of Korean black raspberry marc and/or blueberry marc. The number of oviposition was also tend to increase by adding additional feed to sawdust but there was no significant different.

• Preventive Nutrition and Food Science
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• v.14 no.3
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• pp.188-194
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• 2009

In this study, we evaluated the phytochemical compositions of the fruits of the pear cactus, mulberry, and Korean black raspberry to determine how they related to antioxidant capacity. Specifically, the total soluble polyphenol, flavonoid and anthocyanin contents were determined, and the phenolic compounds were evaluated by HPLC. In addition, the antioxidant capacity was evaluated by electron paramagnetic resonance (EPR), SOD-like activity and nitrate scavenging activity. Korean black raspberry was found to have the highest level of phytochemicals, followed by mulberry and pear cactus (p<0.05). Furthermore, EPR revealed that Korean black raspberry extract (100 ${\mu}g$/mL) removed 78.6% of the superoxide radicals in a xanthin-xanthin oxidase. Additionally, when the removal of hydroxyl radicals was evaluated, the extract of pear cactus showed 50% scavenging activity at 1000 ${\mu}g$/mL. Korean black raspberry extract showed the highest SOD-like activity, which was 44% (p<0.05). Finally, the nitrate scavenging activity of both extracts of mulberry and Korean black raspberry were found to be 93%, while that of the pear cactus was 63% at pH 1.2 (p<0.05).

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.656-661
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• 2005

The study was conducted to investigate per oral (PO) effects of Black raspberry wine on testosterone levels in Sprague-Dawley rats oral administrated with Methoxychlor in order to establish the experimental clinical model for evaluating the influences on the sexual hormones of SD-Rat administrated with Methoxychlor(MET), it was dissolved in acetone and olive oil (1:19), which was administrated orally at doses of 200mg/kg body weight/ day for 7days. Black raspberry wine of 13% alcohol concentration, was prepared from ripen fruits of Rubus coreanus fermented with Saccharomyces cervisiae. PO administration of Black raspberry wine for 15 week produced dramatic increases of serum testosterone levels. Increase in the testosterone level was observed, using gamma counter with 125I testosterone, starting from 1 week post administration. Maximum increase in testosterone level was observed at 4 week post administration, 5.18${\pm}$0.76ng/mL, which was 10.1 times higher than before and at 15 weeks post administration it was recorded as 1.67${\pm}$0.19ng/mL indicating Black raspberry wine as an effective phyto-testosteronic beverage of the future.

• The Korea Journal of Herbology
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• v.35 no.4
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• pp.45-50
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• 2020

Objective : This study was performed to evaluate the toxicity after a single oral administration of black raspberry extract to male and female Sprague-Dawley (SD) rats and to determine the approximate lethal dose (ALD). Methods : We previously showed that the black raspberry extract repressed the simvastatin-mediated expression of Proprotein convertase subtilisin/kexin type 9 (PCSK9) and improved Low-Density Lipoprotein cholesterol (LDL-C) uptake by hepatocytes through the induction of the Low-Density Lipoprotein Receptor expression in hepatocytes. The groups consisted of black raspberry extract groups, as an oral dose of 2,000 mg/kg and a control group. 5 weeks SD rats were randomly assigned to 4 groups of 5 rats. Each male and female SD rats were administered orally once. For 14 days after the administration, mortality, clinical signs, changes in body weight, and necropsy findings were observed according to the "Standard for Toxicity Study of Pharmaceuticals" of Korea Food and Drug Administration (KFDA) guideline and "Acute Oral Toxicity- Fixed Dose Procedure" of OECD Test Guideline. Results : There were no cases of mortality in the group administered with 2,000 mg/kg of male and female, and no abnormalities in body weight change and clinical signs. Also, no gross abnormalities were observed at the autopsy. Conclusions : As a result of a single oral administration of the black raspberry extract to SD rats, the ALD was determined to exceed 2,000 mg/kg for both male and female SD rats.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.92-98
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• 2013

The objective of this study was to establish the decision criteria of soil suitability for Korean Black raspberry using soil morphological and physical properties. The investigation was carried out in Gochang, Sunchang, Jeongeup, Pohang, and Hoengseong districts in Korea. The obtained results showed that factors related to the decision criteria of the soil suitability for Korean Black raspberry cultivation were soil texture, soil drainage class, land slope, and available soil depth. The criteria of the best suitability soil for Korean Black raspberry was valley/fan or hill geomorphology, well or moderately drainage class, B-slope(2-7%), coarse loamy soil texture family, less than <15% gravel contents, and available soil depth deeper than 100cm. The area criteria of soil suitability for Korean Black raspberry of Gochang was more than 50% including best and suitable areas.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.4
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• pp.507-515
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• 2014

Benign prostatic hyperplasia (BPH), which is commonly found in aging men, is characterized by hyperplasia of prostatic stromal and epithelial cells beginning in the periurethral zone of the prostate. The prevalence of BPH increases in an age-dependent manner. Here, we investigated the protective effects of unripe Rubus occidentalis extracts (UROE) on BPH development using a prostate cancer cell line and testosterone-induced BPH rat model. Experiments using an established hormone-dependent prostate cancer cell line (LNCaP) showed that UROE treatment significantly decreased expression of androgen-related genes, including androgen receptor (AR), prostate specific antigen (PSA), and 5-alpha reductase 2, but not 5-alpha reductase 1, which was also observed in flutamide-treated cells. Further, AR and PSA gene expression was reduced by UROE treatment under androgen-stimulated conditions using dihydrotestosterone (DHT). BPH animals displayed elevated prostate weights. However, UROE as well as finasteride treatment significantly reduced prostate weights and DHT levels compared to testosterone-induced BPH animals. Histopathological analysis also showed that UROE treatment suppressed testosterone-induced prostatic hyperplasia. Taken together, the results suggest that UROE may effectively inhibit the development of BPH and thus may be a useful agent in BPH treatment.

• BMB Reports
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• v.41 no.7
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• pp.529-536
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• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme in the phenylpropanoid pathway, which is responsible for synthesizing a variety of secondary metabolites that participate in development and adaptation. In this study, we isolated a full-length cDNA of the C4H gene from the Korean black raspberry (Rubus sp.) and found that this gene existed as a single gene. By comparing the deduced amino acid sequence of Rubus sp. C4H with other sequences reported previously we determined that this sequence was highly conserved among widely divergent plant species. In addition, quantitative real time PCR studies indicated that the C4H gene had a differential expression pattern during fruit development, where gene expression was first detected in green fruit and was then remarkably reduced in yellow fruit, followed by an increase in red and black fruit. To investigate the two peaks in expression observed during fruit development and ripening, we measured the flavonoid content. The content of the major flavanol of Korean black raspberry fruits was determined to be highest at the beginning of fruit development, followed by a gradually decrease according to the developmental stages. In contrast, the content of anthocyanins during the progress of ripening was dramatically increased. Our results suggest that the C4H gene in Korean black raspberry plays a role during color development at the late stages of fruit ripening, whereas the expression of C4H gene during the early stages may be related to the accumulation of flavanols.

• Korean Journal of Food Science and Technology
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• v.40 no.2
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• pp.129-134
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• 2008

Five antioxidative active substances were isolated from the EtOAc layer (20 g/56.2 g) of Korean black raspberry (Rubus coreanus Miquel) wine (11 L, black raspberry 15.7 kg fresh wt. eq.) by various column chromatography and high performance liquid chromatography (HPLC). Proton nuclear magnetic resonance ($^1H$-NMR) spectroscopy and gas chromatography Electro Ionization-Mass Spectrometry (GC-EI-MS) identified these as 4-hydroxybenzoic acid (1, 0.1 mg), 3,4-dihydroxybenzoic acid (2, 0.3 mg), 4-(2-hydroxyethyl)-phenol (3, 0.6 mg; tyrosol), pyrocatechol (4, 0.3 mg), 3,4,5-trihydroxybenzoic acid ethyl ester (5, 0.6 mg; ethyl gallate). The presence of 1 and 2 in Korean black raspberry has previously been reported. However, the presence of 3-5 in Korean black raspberry, and the identification of 1-5 from the Korean black raspberry wine have never before been reported.

• Research in Plant Disease
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• v.19 no.2
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• pp.121-123
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• 2013

The strains of Colletotrichum gloeosporioides, C. coccodes, C. acutatum isolated from black raspberry were pathogenic to apple and strawberry after dropping inoculation, but showed weak pathogenicity in hot-pepper and tomato. The anthracnose pathogens of C. gloeosporioides, C. orbiculare, C. acutatum isolated from apple, hot-pepper and pumpkin showed pathogenicity in black raspberry. Moreover, the anthracnose pathogens isolated from apple caused disease symptoms in non-wounded inoculation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.5
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• pp.3361-3369
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• 2015

The objective of this study was to improve the quality of black raspberry wine added with wild grape, based on selection of yeast strains and fermentation conditions. The Y1 yeast(Lalvin 71B) indicated significantly higher lactic acid, ester, fusel oil content after fermented mashing than other samples tested and had an effect on the reduction of acidity and enhanced aroma of black raspberry wine. In addition, the high fermentation temperature($25^{\circ}C$) using Y1 yeast showed much higher tendency to retain components of aroma (ethyl acetate, higher alcohols) and the highest overall preference including sensory evaluation than that of low fermentation temperture($15^{\circ}C$). Thus, the fermentation using Y1 yeast at $25^{\circ}C$ can be applied to positively improve the taste and flavor of production of black raspberry wine added wild grape.