• Journal of fish pathology
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• v.19 no.3
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• pp.277-284
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• 2006

Application of wood vinegar in fish farms has been used for the disinfection of pathogenic microorganisms and the treatment of infectious diseases. This study was performed to know the acute toxicity of wood vinegar to carp Cyprinus carpio, flounder Paralichyhus olivaceus, rock fish Sebastes schlegeli and black sea bream Pagrus major. The 24 hr, 48 hr and 96 hr LC50 respectively were: carp 1243, 1143 and 1016 ppm; flounder 1397, 1253 and 1226 ppm; rock fish 1058, 993 and 967 ppm; and black sea bream 650, 616 and 596 ppm. Death and survival of fish exposed to lethal concentrations of wood vinegar were apparently related to massive necrosis, fusion and epithelial lifting of gill lamellar epithelium, suggesting the osmotic imbalance and lack of oxygen uptake.

• The Journal of the Acoustical Society of Korea
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• v.25 no.3E
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• pp.101-109
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• 2006

Besides dorsal-aspect target strength (TS) of the fish, side-aspect TS information is also acoustically important parameter in fisheries acoustics. In this study, the side-aspect TS of 11 black sea bream (Acanthopagurus schlegeli) were measured using a split beam echosounder of 120, 200, and 420 kHz; total length of the fish ranged from 12.4 to 23.7 cm, and wet weight from 27.5 to 229.8g. For the precise TS measurement with side-aspect angle, we used anesthetized and tethered specimens of known size while being rotated through $360^{\circ}C$ by means of a carousel structure. The side-aspect TS measurements of the fish were conducted by rotating the fish in the horizontal plane at 50 interval. The ping interval was 0.2 second and elapsed time at each angle was 30-60 second. As a result, the measured side-aspect TS data were fitted by sinusoidal function. The relationships between fish length and near full side-aspect TS were as follows: $TS_{120kHz}= 21.46 log (TL)-67.5 (r = 0.70), \;TS_{200 kHz}= 31.03 log (TL)-76.9 (r=0.83),\;TS_{420 kHz}= 30.79 log (TL)-72.2 (r = 0.77)$. For comparison, theoretically estimated side-aspect TS from the Kirchhoff ray mode (KRM) model, which based on swimbladder and body morphology, were compared with the measured TS.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.21 no.1
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• pp.35-40
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• 1985

The author carried out experiments to find out the response of rock bream, Oplegnathus fasciatus (TEMMINCK et SCHLEGEL) and sea bass, Epinephelus septemfasciatus (THUNBERG) to the color nettings. The experimental water tank(180L$\times$50W$\times$55Hcm) was set up n a dark room and water level was maintained 50cm high from the bottom. The tank was devided three longitudinal sections marking 60 cm interval. The illumination systems, consisted of 20 watt fluorescent lamps and filter, were suspended adove the tank. Two different color nettings selected from five colors (red, yellow, green, blue, black) were placed in each end section of the tank. Ten fish were used in each experiment and the fish were acclimatized in the dark for 60 minutes before experiment. After the light on, the number of fish in each section of the tank was counted in every 30 seconds interval for 30 minutes. The results obtained are as follows: 1. The rock bream selected the color nettings in the order of yellow, black, blue, green and red. 2. The sea bass selected the color nettings in the order of green, black, red, blue and yellow.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.2
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• pp.66-71
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• 2006

Fluoroquinoles have a wide range of antimicrobial properties and are effective in the treatment of bacterial diseases in fish. The use of fluoroquinoles continues to grow steadily. Fluoroquinolone antibiotics are probably the most important class used among synthetic antibiotics in human and veterinary medicines because of their broad activity spectrum and good oral absorption. This study was conducted to estimate the residue of antibiotics in four species of farmed fishes, including olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major), and sea bass (Lateolabrax japonicus), collected from fish farms located in the southern coastal area of Korea. The residues of fluoroquinolones were determined using high performance liquid chromatography (HPLC) with a fluorescence detector. Residuals of five fluoroquinolones in muscle tissue of farmed fish were analyzed. We found that enrofloxacin was the most common agent in fish muscle, and that ciprofloxacin was the next most common. The range of detected concentrations of fluoroquinolones in olive flounder muscle was 0-0.859 mg/kg in 32.6% of all samples. Enrofloxacin was commonly detected in sea bass muscle at a range of 0-0.143 mg/kg in 38.9% of all samples. Fluoroquinolones were detected in 6.9% of black rock fish muscle and in 16.6% of sea bream, although the detected concentration was below 0.01 mg/kg. The maximum detection value of enrofloxacin and ofloxacin in olive flounder at the time of shipping was 0.102 mg/kg and 0.09 mg/kg, respectively; no other antimicrobials were detected. We detected no antimicrobial substances in red sea bream.

• Journal of Aquaculture
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• v.20 no.2
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• pp.90-95
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• 2007

Artificially-produced juvenile black sea bream, Acanthopagrus schlegeli (total length $10.4{\pm}1.1\;cm$, body weight $18.7{\pm}3.4\;g$) were released into the Gamak Bay in Korea. And their initial feeding habits and body compositions were compared with those of cultured fish (CUL) for a period of one month after release. The released black sea bream commenced feeding on the 1st day after release, and predation rate was more than 80% during the sampling period. The stomach content of fish was composed of mainly fishing bait and krill in fish sampled on 15th days after release; however, its content changed to the natural organisms such as algae and small crustaceans when the fish was sampled on 20th days after release. Crude lipid content of carcass of fish in the sampling station A (SA) were lower than that of fish in the initial, sampling station B (SB) and CUL for the 5th and 10th days after release. Carcass n-3 HUFA composition, total amino acid and essential amino acid contents of released fish showed high trend compared to that of cultured fish in 30th days after release. The released fish have the ability to adjust their feeding habits and biochemical metabolism to the natural environment within a short period even though they showed preference to artificial feed in the early days after release.

• Journal of fish pathology
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• v.21 no.2
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• pp.139-148
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• 2008

Vertebral deformity in hatchery-reared black sea bream (Acanthoparus schlegelii) fingerlings occurred. Deformed fish had a good appetite but no clinical signs were found except the vertebral abnormality and darkness around the dorsal skin of the deformed vertebra. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. No morphological change in the swim bladder was observed. Histopathological changes on the deformed vertebra, gill, liver, and spleen were observed to clarify the cause of the deformity. The vertebra were irregularly deformed upward with the shape of "V" in an alphabetic character causing the spinal cord and dorsal aorta suppressed. The diameters of the muscle fibers around the deformed vertebra, especially, in the dorsal part, were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were enlarged. No evidence of inflammatory responses in the lateral musculature were found. On the basis of normal inflation of the swim bladder, cultural environments for growth of the fingerlings, and histopathological alterations in the muscle, vertebra and gills, it is suggested that high speed of water current in the culturing aquaria between 23 to 30 days after hatching was responsible for the development of vertebral abnormality.

• Journal of fish pathology
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• v.26 no.2
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• pp.99-110
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• 2013

Hatchery-reared seeds provides a key source of animal protein for human consumption and restocking for fishery management. For stock enhancement program, we have inspected the hatchery-reared seeds of 33 species in 2009, 44 species in 2010, 43 species in 2011 and 46 species in 2012 for legally designated diseases. Results showed that abalone was the most abundant in the marine species group and then sea cucumber, olive flounder, rockfish and swimming crab were followed. Crucian carp was the most abundant and then mandarin fish, Korean bullhead, melanian snail and Chinese mitten crab were followed in the freshwater species group. The number of inspection for black sea bream, rock bream, scorpionfish, black scraper, and eel has continuously decreased for four years. The inspection for flathead mullet has been carried out only in 2009. The total number of inspection cases for eight pathogens in this study were 8,476 and disqualification cases were 56 (0.67%) by detection of aquatic animals pathogens such as koi herpesvirus, white spot syndrome virus, red sea bream iridovirus or viral haemorrhagic septicemia virus.

• Journal of fish pathology
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• v.23 no.1
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• pp.85-98
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• 2010

Ministry of Agriculture, Fisheries and Food (MAFF) and National Fisheries Research and Development Institute (NFRDI) have inspected the hatchery-reared seeds of 22 marine species and 11 freshwater species for aquatic animal diseases in stock enhancement program in 2009. Results showed that total 12 local selfgovernments have been restocking the sea with cultured juveniles. Gyeongsangnam-do, Jeollanam-do, Jejudo and Chungcheongnam-do have a preference for marine species seeds to freshwater species. On the contrary, freshwater species were released mostly in Gyeonggi-do, Jeollabuk-do and Chungcheongbuk-do. In the marine species group, abalone was the most abundant as 24.5%, and then sea cucumber (15.2%), olive flounder (11.5%), swimming crab (5.6%), black sea bream and rockfish (6.8%), rock bream (5.1%), black rockfish (4.6%) and scorpionfish (4.5%) were followed. Crucian carp was the most abundant as 19.4%, and then eel (17.0%), Korean bullhead (12.3%), melanian snail (12.0%), catfish (8.4%) were followed in the freshwater species group. The total number of inspection cases in this study were 1,080 and disqualification cases were 19 by detection of aquatic animals pathogens such as red sea bream iridovirus (RSIV), koi herpesvirus (KHV) or white spot syndrome virus (WSSV).

• Journal of Aquaculture
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• v.21 no.4
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• pp.278-284
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• 2008

Artificially-produced juvenile black sea bream Acanthopagrus schlegeli (total length $8.3{\pm}1.0\;cm$, body weight $11.2{\pm}4.2\;g$) were released in eelgrass, Zostera marina bed and their initial food organism and changes of body compositions were investigated for one month after release. Predation rates in fish sampled on 1st and 3rd days were 79%, increased up to 100% on 7th and 10th days, and then decreased on 30th days as 75%. Major prey organisms was composed of mainly Amphipoda and Gastropoda. Ratio of Amphipoda and Gastropoda in stomach were highest in fish sampled on 15th and 1st days after release, respectively. Crustacea and Algae were maintained about 20% during a sampling period. Visceral weight index (VWI) offish sampled 20th and 30th after release were significantly higher than that of initial. Carcass crude protein and lipid contents of released fish were showed significantly decreasing; however carcass n-3 HUFA composition was showed increasing tendency with the passage of time after release. Eelgrass bed was supposed to be helpful for the released fish to adjust their feeding habits and biochemical metabolism to the natural environment within a short period after release.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.2
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• pp.109-117
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• 1997

In order to hold the behavior of fish school to the set-net, a series of tag-recapture experiments were carried out in two fishing grounds of the middle sized set-nets which were located in 20m depth on the coast of Keojedo and Namhaedo in the Southern part of Korea from September to October in 1996. In the experiments, the leading ability of the leader and the fish court and the recapturing ability of the bag nets were checked out for the six species of fish in method of discharging the tagged fishes at side points of leader, and the middle points of the fish court and the bag nets in a hauling step, and recapturing them at the bag nets in the next hauling. The results obtained are as follows; 1. The ratio recaptured at the both side bag-nets in the next hauling after discharged from the fish court in the previous step was 20.3% in small size of mackerel Scomber japonicus, 16.2% in small size of horse mackerel Trachurus japonicus, 10.3% in black sea-bream Acanthopagrus schlegelii, 19.1% in red barracuda Syhyraena pinguis, 16.3% in halfbeak Hemiramphus sajori, 20.0% in gizzard shad Konosirus punctatus individually, and totally in six species of fish, that was 17.2%. 2. The ratio recaptured at the same bag net after discharged in the both side bag-nets was 21.7% in small size of mackerel, 21.5% in small size of horse mackerel, 6.7% in black sea-bream, 17.8% in red barracuda, 16.8 in half-beak, 19.1% in gizzard shad individually, and totally in six species of fish, that was 18.8%. 3. The leading ratio from side points of the leader departed from door in 25m to fish court was 58.9% in small size of mackerel, 74.6% in small size of horse mackerel, 38.0% in black sea-bream, 54.7% in red barracuda, 58.6% in half-beak, 54.5% in gizzard shad individually, and totally in six species of fish, that was 57.8%. So it was assumed that the leader of set-net was very effective in leading to the swimming direction of small size of mackerel, small size of horse mackerel, red barracuda, half-beak and gizzard shad. 4. Red barracuda, half-beak and gizzard shad entered into bag net of upstream in large numbers than bag net of downstream, and small size of horse mackerel and black sea-bream entered into bag net of downstream in large numbers than bag net of upstream. 5. Small size of mackerel and small size of horse mackerel had high remaining rate in the bag net of downstream, and black sea-bream, red barracuda and half-beak had high remaining rate in the bag net of upstream.