• BMB Reports
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• v.41 no.4
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• pp.310-315
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• 2008

BirA ligase is a prokaryotic ortholog of holocarboxylase synthetase (HCS) that can biotinylate proteins. This study tested the hypothesis that BirA ligase catalyzes the biotinylation of eukaryotic histones. If so, this would mean that recombinant BirA ligase is a useful surrogate for HCS in studies of histone biotinylation. The biological activity of recombinant BirA ligase was confirmed by enzymatic biotinylation of p67. In particular, it was found that BirA ligase biotinylated both calf thymus histone H1 and human bulk histone extracts. Incubation of recombinant BirA ligase with H3-based synthetic peptides showed that lysines 4, 9, 18, and 23 in histone H3 are the targets for the biotinylation by BirA ligase. Modification of the peptides (e.g., serine phosphorylation) affected the subsequent biotinylation by BirA ligase, suggesting crosstalk between modifications. In conclusion, this study suggests that prokaryotic BirA ligase is a promiscuous enzyme and biotinylates eukaryotic histones. Moreover the biotinylation of histones by BirA ligase is consistent with the proposed role of human HCS in chromatin.

• Wind and Structures
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• v.25 no.1
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• pp.39-61
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• 2017

Buffeting induced resonance (BIR) of hangers on long-suspension bridges is briefly reviewed, including mechanism and experimental verification. Taken the Xihoumen suspension bridge as a numerical example, sensitivities of the BIR of hangers to wind properties are investigated, including types of wind spectrum, turbulence intensity, and spacial coherence of wind fluctuations. Numerical simulations indicate that the BIR of hangers occur to both cases of different wind spectra, showing that it is insensitive to types of wind spectrum. On the other hand, it is found that the turbulence intensity affects buffeting of main cables almost in a linear manner, and so it does to the BIR of the hangers; however, the resonance factors, namely the ratio of the response of the hanger to that of the main cable, are little affected by the turbulence intensity. The spacial coherence of the wind fluctuations, although plays an important role on the buffeting responses of the main structure, has no substantial effects on the BIR of the hangers. Finally, replacement of steel strand with CFRP material has been verified as a very effective countermeasure against the BIR of hangers.

• Journal of KIISE:Software and Applications
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• v.34 no.8
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• pp.743-751
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• 2007

Model checking has been successfully applied to hardware verification. Software is more subtle than hardware with respect to formal verification due to its infinite state space. Although there are many research activities in this area, bounded model checking is regarded as a promising technique. Bounded model checking uses an upper bound to unroll its model, which is the main advantage of bounded model checking compared to other model checking techniques. In this paper, we applied bounded model checking to verify BIR which is the input model for the model checking tool BOGOR. Some BIR examples are verified with our technique. Experimental results show that bounded model checking is better than explicit model checking provided by BOGOR. This paper presents the formalization of BIR and the encoding algorithm of BIR into CNF.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.4
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• pp.247-256
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• 2013

In this study, alginate beads containing birnessite (Bir-AB), a highly reactive oxidative catalyst for the transformation of phenolic compounds, was prepared and its 1-naphthol (1-NP) removal efficiency was investigated in a batch test. Based on scanning electron microscopy image, it can be inferred that the alginate gel cluster acts as a bridge which bind the birnessite particles together. Kinetic experiment with Bir-AB of different mixing ratios of birnessite to alginate (Bir : AG=0.25 : 1~1 : 1 w/w) indicate that pseudo-first order kinetic constants, $k(hr^{-1})$ for the 1-NP removals increased about 1.5 times when the birnessite mixing ratio was doubled. The removals of 1-NP was found to be dependent on solution pH and the pesudo-first order rate constants were increased from 0.331 $hr^{-1}$ at pH 10 to 0.661 $hr^{-1}$ at pH 4. The analysis of total organic carbon for the reaction solutions showed that a higher removal of dissolved organic carbon was achieved with Bir-AB as compared to birnessite. HPLC chromatographic analysis of the methanol extract after reaction of 1-NP with Bir-AB suggest that the reaction products could be removed through incorporation into the aliginate beads as a bound residue. Mn ions produced from the oxidative transformation of 1-NP by birnessite were also removed by sorption to Bir-AB. The Bir-AB was recovered quantitatively by simple filtration and was reused twice without significant loss of the initial reactivity.

• Animal cells and systems
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• v.9 no.4
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• pp.181-190
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• 2005

BIRPs (BIR containing Proteins) which contain one to three BIR domains constitute a highly conserved family from yeast to human. BIR domains mediate the interaction of BIRPs with various other proteins. Some of the members acquire a Ring domain which acts as an E3 ubiquitin ligase. The first member of BIRPs identified in the baculovirus was found as an inhibitor of apoptosis and most of the family members in the other species have been recognized to have the same function which bind to and inhibit caspases, thereby suppresses apoptotic cell death. But an increasing number of evidences indicate that BIRPs are involved in various cellular events such as cell division, control of cell cycle, signal transduction, cell migration, innate immunity as well as regulation of apoptosis. In this review, we summarize the structural and functional features of the BIRPs, especially focus on the various functions of BIRPs unrelated to regulation of apoptosis by the recent findings.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1720-1721
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• 2007

This paper presents a Fuzzy+PID control method for a Bridge Inspection Robot(BIR) system. The BIR has been developed with the aim of checking the safety status of a real bridge, gathering accurate data and performing maintenance. The developed robot system is composed of the specially designed car for bridge inspection, the guide rail and the inspection robot. The proposed Fuzzy+PID controllers are used to track speed reference signal of X axis and position reference signal of Z axis. Experimental results verify that the proposed Fuzz+PID control design method can achieve favorable control performance with regard to external disturbance.

• Journal of IKEEE
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• v.26 no.1
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• pp.36-42
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• 2022

This study presents an analysis of the human body channel as an electric signal path using body impulse response (BIR). The human body communications (HBC) has recently emerged as an effective signal transmission method to create wireless body area networks (WBAN). We provide body channel characteristics based on measured BIR in a proper experimental environment for the HBC using capacitive coupling with a customized channel sounding device, which can be applied as a guideline for the HBC system design. The frequency response of the BIR, extracted by a customized signal processing for the measure signals, shows the channel path loss (CPS) between 0 MHz and 100 MHz with an average CPS of approximately 46.8 dB. In addition, the relative noise power distributions can provide estimations on the signal to noise ratio at the HBC receiver in terms of capacitor and resistor values in the measured frequency band and the frequency band lower than 3 MHz considering the baseband signal detection.

• Molecular & Cellular Toxicology
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• v.4 no.4
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• pp.278-284
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• 2008

The X-linked inhibitor of apoptosis (XIAP) is a member of a novel family of inhibitors of apoptosis and has several BIR domains (BIR1, BIR2, and BIR3) and a carboxy-terminal RING zinc-finger. Since suppressionof apoptosis is fundamentally important for carcinogenesis and tumor growth, we investigated the expression and function of XIAP in DEN-induced carcinogenesis using rat model. Wistar rats were injected intraperitoneally with DEN at a dose of 50 mg/kg in twice a week for 12 weeks (Group II) and 16 weeks (Group III) followed by the recovery periods, respectively. The evaluation of DEN-induced carcinogenesis carried out the blood, RT-PCR, histopathological and western blot analysis. The level of blood chemistry including GOT/GPT, albumin, and total bilirubin were significantly exchanged comparing to control and Group I/Group II. The expression of albumin and collagen mRNA were significantly exchanged (P<0.05) in both groups. In addition, AFP mRNA expression decreased more after recovery periods than Group II. XIAP was expressed constitutively in normal rat liver as well as DEN-induced Groups I and Group II. In addition, XIAP expression increased more in Group I with 4 weeks recovery periods than Group I. However, XIAP expression shown to increase in Group lI, otherwise, it was decreased in Group II with 10 weeks repair periods. Taken together, these results suggest the alteration of XIAP expression could be involved in hepatocellular carcinogenesis.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.543-554
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• 2021

To preventively control fire blight in apple trees and determine policies regarding field monitoring, the Maryblyt ver. 7.1 model (MARYBLYT) was evaluated in the cities of Chungju, Jecheon, and Eumseong in Korea from 2015 to 2020. The number of blossom infection alerts was the highest in 2020 and the lowest in 2017 and 2018. And the common feature of MARYBLYT blossom infection risks during the flowering period was that the time of BIR-High or BIR-Infection alerts was the same regardless of location. The flowering periods of the trees required to operate the model varied according to the year and geographic location. The model predicts the risk of "Infection" during the flowering periods, and recommends the appropriate times to control blossom infection. In 2020, when flower blight was severe, the difference between the expected date of blossom blight symptoms presented by MARYBLYT and the date of actual symptom detection was only 1-3 days, implying that MARYBLYT is highly accurate. As the model was originally developed based on data obtained from the eastern region of the United States, which has a climate similar to that of Korea, this model can be used in Korea. To improve field utilization, however, the entire flowering period of multiple apple varieties needs to be considered when the model is applied. MARYBLYT is believed to be a useful tool for determining when to control and monitor apple cultivation areas that suffer from serious fire blight problems.

• Biodesign
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• v.6 no.4
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• pp.92-95
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• 2018

API5 is a unique oncogenic, non-BIR type IAP nuclear protein and is up-regulated in several cancers. It exerts several functions, such as apoptosis inhibition, cell cycle progression, cancer immune escape, and anticancer drug resistance. Although structural studies of API have revealed that API5 mediates protein-protein interactions, its detailed molecular functions remain unknown. Since FGF2 is one of API5's major interacting proteins, structural studies of the API5-FGF2 complex will provide insight into both proteins' molecular function. We overexpressed and purified API5 and FGF2 in Escherichia coli and crystallized the API-FGF2 complex using polyethylene glycol (PEG) 6000 as a precipitant. Diffraction data were collected to a $2.7{\AA}$ resolution using synchrotron X-rays. Preliminary diffraction analysis revealed that the API5-FGF2 complex crystal belongs to the space group $P2_12_12_1$ with the following unit cell parameters: a = 46.862, b = 76.523, $c=208.161{\AA}$. One asymmetric unit with 49.9% solvent contains one API5-FGF2 complex. Molecular replacement calculation, using API5 and FGF2 coordinates, provided a clear electron density map for an API5-FGF2 complex.