• Journal of Electrochemical Science and Technology
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• 제14권1호
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• pp.1-14
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• 2023

The overuse of pesticides in agricultural sectors exposes people to food contamination. Pesticides are toxic to humans and can have both acute and chronic health effects. To protect food consumers from the adverse effects of pesticides, a rapid monitoring system of the residues is in dire need. Molecularly imprinted polymer (MIP) on a screen-printed electrode (SPE) is a leading and promising electrochemical sensing approach for the detection of several residues including pesticides. Despite the huge development in analytical instrumentation developed for contaminant detection in recent years such as HPLC and GC/MS, these conventional techniques are time-consuming and labor-intensive. Additionally, the imprinted SPE detection system offers a simple portable setup where all electrodes are integrated into a single strip, and a more affordable approach compared to MIP attached to traditional rod electrodes. Recently, numerous reviews have been published on the production and sensing applications of MIPs however, the research field lacks reviews on the use of MIPs on electrochemical sensors utilizing the SPE technology. This paper presents a distinguished overview of the MIP technique used on bare and modified SPEs for the detection of pesticides from four recent publications which are malathion, chlorpyrifos, paraoxon and cyhexatin. Different molecular imprint routes were used to prepare these biomimetic sensors including solution polymerization, thermal polymerization, and electropolymerization. The unique characteristics of each MIP-modified SPE are discussed and the comparison among the findings of the papers is critically reviewed.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.55-55
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• 2015

Today, engineers are facing new set of challenges that are quite different from the conventional ones. Information technologies are rapidly commoditizing while the paths beyond the current roadmaps became uncertain as various technologies have been pushed to their limits. Along with these changes in IT ecosystems, grand challenges such as global security, health, sustainability, and energy increasingly require trans-disciplinary solutions that go beyond the traditional arenas in STEM (Science, Technology, Engineering and Mathematics). Addressing these needs is shifting engineering education and research to a new paradigm where the emphasis is placed on the consilience for holistic and system level understanding and the convergence of technology with AHSD (arts, humanities, social science, and design). At the center of this evolutionary convergence, nanotechnologies are enabling novel functionalities such as bio-compatibility, flexibility, low power, and sustainability while on a mission to meet scalability and low cost for smart electronics, u-health, sensing networks, and self-sustainable energy systems. This talk introduces the efforts of convergence based on the emerging nano technology tool sets in the newly launched School of Integrated Technology and the Yonsei Institute of Convergence Technology at Yonsei International Campus. While the conventional devices have largely depended upon the inherent material properties, the newer devices are enabled by nanoscale dimensions and structures in increasingly standardized and scalable fabrication platform. Localized surface plasmon resonance in 0 dimensional nano particles and structures leads to subwavelength confinement and enhanced near-field interactions enabling novel field of metal photonics for sensing and integrated photonic applications [1,2]. Unique properties offered by 1 dimensional nanowires and 2 dimensional materials and structures can enable novel electronic, photonic, nano-bio, and biomimetic applications [3-5]. These novel functionalities offered by the emerging nanotechnologies are continuously finding pathways to be part of smart systems to improve the overall quality of life.

• 대한기계학회논문집B
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• 제38권10호
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• pp.817-829
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• 2014

조직공학 분야에서의 3 차원 구조체는 세포의 성장과 분화를 유도하기 위한 미세 환경을 제공하고, 재생하고자 하는 조직의 형태를 유지할 수 있도록 지탱해 주는 역할을 수행한다. 현재까지 다양한 생체재료 및 이의 가공 기법들이 이러한 3 차원 구조체를 제작하는데 적용되고 있다. 특히, 3 차원 프린팅 기술은 다양한 재료를 이용하여 원하는 외부 형상과 내부 구조를 제작할 수 있기 때문에 오늘날 조직공학 분야에 많이 이용되고 있고, 이 기술을 통해 새로운 조직공학적 접근 방법도 시도되고 있다. 본 논문에서는, 현재 조직공학 분야에 적용되고 있는 3 차원 프린팅 기술과, 이를 통해 제작된 기능성 인공지지체 및 세포 프린팅 구조체, 그리고 이의 다양한 조직공학적 적용에 대해서 서술하고자 한다.

• Journal of Periodontal and Implant Science
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• 제42권4호
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• pp.113-118
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• 2012

Purpose: The aim of this study was to investigate the effects of synthetic fibronectin (FN) fragments, including fibrin binding sites from amino-terminal FN fragments containing type I repeats 1 to 5, on osteoblast-like cell activity. Methods: Oligopeptides ranging from 9 to 20 amino acids, designated FF1, FF3, and FF5, were synthesized by a solid-phase peptide synthesizing system, and we investigated the effects of these peptides on cell attachment and extent of mineralization using confocal microscopy, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, and Alizarin red S staining. Results: FF3 and FF5 peptides increased the number of attached human osteoblastic cells, and FF3 administration led to prominent cell spreading. Mineralization was increased in FF3 and FF5 compared to FF1 and the untreated control. Conclusions: Taken together, it can be concluded that the fibrin-binding oligopeptides FF3 and FF5 enhanced cell attachment and mineralization on osteoblast-like cells. These results indicate that FF3 and FF5 have the potential to increase osteoblast-like cell activity. Performing an in vivo study may provide further possibilities for surface modification of biomimetic peptides to enhance osteogenesis, thus improving the regeneration of destroyed alveolar bone.

• 대한기계학회논문집A
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• 제34권9호
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• pp.1145-1151
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• 2010

본 논문은 형상기억합금 와이어에 의해 구동되는 점핑로봇에 대한 연구로서 기구의 설계와 컴퓨터 시뮬레이션을 포함하고 있다. 인간과 같은 척추동물 하지 근골격계의 구조와 기능을 모방한 구조의 점핑 기구를 설계하였다. 점핑 기구의 각 다리는 대퇴부, 정강이, 발의 세 부분으로 구성되고, 점핑에 필수적인 단일관절근육인 대둔근, 양관절근육인 대퇴직근과 비복근을 포함하는 구조이다. 각 근육을 형상기억합금 와이어로 대체한 컴퓨터 모델로 시뮬레이션한 결과, 로봇의 최대 점핑 높이가 로봇 신장의 약 4 배임을 확인하였다. 또한 구조가 보다 단순화된 로봇 모델과 점핑 성능을 비교하였고, 그 결과 근골격계를 모방한 모델이 점핑 높이 면에서 3.3 배 우수한 것으로 나타났다. 이러한 컴퓨터 시뮬레이션을 통하여 형상기억합금이 소형 점핑로봇의 작동기로 사용하기에 적합함을 확인하였다.

• 한국세라믹학회지
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• 제48권3호
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• pp.217-221
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• 2011

Glass-ceramic materials, which consist of glass matrix phase containing crystalline ${\beta}-Ca_3(PO_4)_2$ and ${\beta}-Ca_2P_2O_7$, have been prepared by heating at $750-900^{\circ}C$ of calcium phosphate invert glasses in the silica-free $CaO-P_2O_5-TiO_2-Na_2O$, system. With increasing heating temperature from 750 to $900^{\circ}C$, the crystallite size of precipitated ${\beta}-Ca_3(PO_4)_2$ in glass with $55CaO{\cdot}35P_2O_5{\cdot}3TiO_2{\cdot}7Na_2O$ (mol%) composition increased from 48 to 91 nm. With the extension of the immersion time in dilute acetic acid solution (pH = 5) to ${\geq}$200 min, the degree of dissolution of $Ca^{2+}$ and $P^{5+}$ ions in the glass-ceramics was linearly increased and the solution was constantly maintained at pH = ~7. Biomimetic nanostructured (62-88 nm in average dia.), sphere-shaped hydroxyapatite was homogeneously formed on the surface of the glass-ceramics when socked for 7-14 days in a Hanks' solution, indicating bioactivity of the prepared glass-ceramics.

• The Korean Journal of Physiology and Pharmacology
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• 제24권6호
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• pp.441-452
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• 2020

In vivo animal models are limited in their ability to mimic the extremely complex systems of the human body, and there is increasing disquiet about the ethics of animal research. Many authorities in different geographical areas are considering implementing a ban on animal testing, including testing for cosmetics and pharmaceuticals. Therefore, there is a need for research into systems that can replicate the responses of laboratory animals and simulate environments similar to the human body in a laboratory. An in vitro two-dimensional cell culture model is widely used, because such a system is relatively inexpensive, easy to implement, and can gather considerable amounts of reference data. However, these models lack a real physiological extracellular environment. Recent advances in stem cell biology, tissue engineering, and microfabrication techniques have facilitated the development of various 3D cell culture models. These include multicellular spheroids, organoids, and organs-on-chips, each of which has its own advantages and limitations. Organoids are organ-specific cell clusters created by aggregating cells derived from pluripotent, adult, and cancer stem cells. Patient-derived organoids can be used as models of human disease in a culture dish. Biomimetic organ chips are models that replicate the physiological and mechanical functions of human organs. Many organoids and organ-on-a-chips have been developed for drug screening and testing, so competition for patents between countries is also intensifying. We analyzed the scientific and technological trends underlying these cutting-edge models, which are developed for use as non-animal models for testing safety and efficacy at the nonclinical stages of drug development.

• 방사선산업학회지
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• 제7권1호
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• pp.45-49
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• 2013

In tissue engineering application, a fibrous structure of scaffolds has been issued as an alternative system to regulate cell survival and tissue regeneration, and electrospinning technique has been popularly used to generate fibrous meshes or sheets mimicking the structure of native extracellular matrix (ECM). However, recent strategy in the scaffold development is expanded to provide the structural property as well as a biological property of native ECM, a variety of surface modification techniques have been used to introduce biological property. In this study, we developed biomimetic poly(L-lactide-co-${\varepsilon}$-caprolactone) (PLCL) nano- and micro-fibrous scaffolds as a unique platform with structural and biological properties with native ECM using electrospinning method and gamma-ray irradiation. Surface morphology of the scaffolds was observed by scanning electron microscopy, and alteration of surface property was evaluated with toluidine blue O staining, water contact angle measurement and ATR-FTIR analysis.

• 드라이브 ㆍ 컨트롤
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• 제18권1호
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• pp.1-8
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• 2021

This paper presents research on methods for the reduction of forklifts' noise level for the increased comfort and safety of its operator. A cooling fan with a high air volume flow rate installed in the forklift acts as an important design parameter which efficiently cools the heat exchanger system, helping to transfer internal heat from the engine room to the outdoors with both transmitted and diffracted opening noises. The cooling fan contributes significantly to both the forklift's emitted sound power and the operator room's noise level, thereby necessitating research on the forklift's reduction of acoustic power level and transmission. A noise analysis for various fan models with a biomimetic design based on eagle-wing geometry was conducted. In addition to the acoustic power generation, the aerodynamic performance of the cooling blade is also strongly influenced by the design of airfoil distribution, thereby requiring optimization. The cooling fans were fabricated and installed in the forklift in order to check the efficacy of the forklift engine's cooling, and the final version of the fan was measured for its ability to lower acoustic power level and cool the engine room. This study explains the aerodynamic and acoustic features of the designed fans with the use of BEM analysis and forklift test results.

• 대한화장품학회지
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• 제50권2호
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• pp.119-129
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• 2024

인체 피부 림프계는 간질액을 배출하고 면역 시스템을 활성화하는 중요한 역할을 한다. 자외선과 자연적인 노화와 같은 환경 요인들은 종종 이러한 림프관의 구조적 변화를 일으키며, 이로 인해 피부 기능 장애를 발생시키기도 한다. 그러나 이러한 연구를 위한 동물 실험 대체 방안이 없기 때문에 여전히 연구를 진행하기엔 적합하지 않은 제한 사항들이 존재한다. 인체 피부 림프계를 더 잘 이해하고 림프관 형성에 관련된 분자 및 생리학적 변화를 조사하기 위해, 생체 모방 미세유체 플랫폼인 'skin-lymph-on-a-chip'을 제작하여, 새로운 체외 인체 피부 림프 모델을 개발하였다. 간단히 말해, 이 플랫폼은 체외에서 분화된 일차 정상 인간 표피 각질형성세포(NHEKs)와 피부 림프 내피세포(HDLECs)를 공동 배양하는 것을 의미한다. 약 500 시간 동안 자연 발효를 통해 확보하고 집약된 인삼 뿌리 추출물인 Lymphanax^TM의 림프관 형성 효과를 평가하기 위해 해당 시스템에 적용하였고, 분자 수준 요인들의 변화는 딥러닝 기반 알고리즘을 사용하여 분석하였다. 결론적으로, Lymphanax^TM는 skin-lymph-on-a-chip에서 건강한 림프관 형성을 촉진하였고, 그 성분들이 칩내에서 분화된 NHEKs를 거의 침투하지 않는 결과를 통해, HDELCs에 간접적으로 영향을 미쳤음을 확인하였다. 전반적으로, 이 연구는 기존과 차별화된 체외 인체 피부 림프모델 시스템의 확보와 더불어 이를 통한 Lymphanax^TM의 림프 활성화 효과에 대한 새로운 관점을 제공한다.