• 한국작물학회지
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• 제45권1호
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• pp.6-13
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• 2000

Water deficit is the primary constraint of soybean [Glycine max (L.) Merr.] yield, and a physiological understanding of processes affected by water deficit is a key step in identifying and improving drought tolerance in soybean. The objectives of this research were to evaluate biomass and nitrogen accumulation patterns and water use efficiency (WUE) as possible mechanisms associated with the drought tolerance of Jackson. Biomass accumulation of Jackson was contrasted with the PI416937, which also has demonstrated tolerance to drought. For water-deficit treatment, total biomass accumulation was negligible for PI416937, but biomass accumulation continued at approximately 64 % of the well-watered treatment of Jackson. Transpirational losses for Jackson and PI416937 were approximately the same for the water-deficit treatment, indicating that Jackson had superior WUE. Isotopic discrimination of $^{13}$ C relative to $^{12}$ C also indicated that Jackson had higher WUE. Results indicated that increased WUE for Jackson under water deficit showed it was tolerant to drought rather than had an avoidance mechanism.

• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.646-651
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• 2011

Cultures of Saccharomyces cerevisiae were treated with pulsed electric fields to improve accumulation of zinc in the biomass. Under optimized conditions, that is, on 15 min exposure of the 20 h grown culture to PEFs of 1500 V and 10 ${\mu}s$ pulse width, accumulation of zinc in the yeast biomass reached a maximum of 15.57 mg/g d.m. Under optimum zinc concentration (100 ${\mu}g$/ml nutrient medium), its accumulation in the cells was higher by 63% in comparison with the control (without PEFs). That accumulation significantly correlated against zinc concentration in the medium. Neither multiple exposure of the cultures to PEFs nor intermittent supplementation of the cultures with zinc increased the zinc accumulation. The intermittent supplementation of the cultures with zinc and multiple exposures on PEFs could even reduce the accumulation efficiency, respectively, by 57% and 47%.

• Plant Biotechnology Reports
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• 제2권2호
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• pp.133-136
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• 2008

Cell cultures of Commiphora wightii (Arnott.) Bhandari were grown in shake flasks and a bioreactor and an increase in guggulsterone accumulation up to $18{\mu}g\;l^{-1}$ was recorded in cells grown in the production medium containing a combination of sucrose:glucose (4% total), precursors (phenylalanine, pyruvic acid, xylose, and sodium acetate), morphactin, and 2iP. A yield of $10g\;l^{-1}$ biomass and ${\sim}200{\mu}g\;l^{-1}$ guggulsterone was recorded in a 3-l flask and in a 2-l stirred tank bioreactor compared with 6.6 g biomass and $67{\mu}g\;l^{-1}$ guggulsterone in 250-ml flasks. Increased vessel size was correlated with increased biomass and guggulsterone accumulation. 2iP alone was not effective for biomass and guggulsterone accumulation in cell cultures of C. wightii.

• Journal of Forest and Environmental Science
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• 제34권1호
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• pp.12-23
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• 2018

Quantitative information on biomass and available nutrients are essential for developing sustainable forest management strategies to regulate atmospheric carbon. An attempt was made at Chilapatta Reserve Forest in Duars region of West Bengal to quantify its above and below ground carbon along with available "N", "P" and "K" in the soil. Stratified random nested quadrats were marked for soil, biomass and litter sampling. Indirect or non-destructive procedures were employed for biomass estimation. The amount of these available nutrients and organic carbon quantified in soil indicates that the forest soil is high in organic carbon and available "K" and medium in phosphorus and nitrogen. The biomass, soil carbon and total carbon (soil C＋C in plant biomass) in the forest was 1,995.98, 75.83 and $973.65Mg\;ha^{-1}$. More than 90% of the carbon accumulated in the forest was contributed by the trees. The annual litter production of the forest was $5.37Mg\;ha^{-1}$. Carbon accumulation is intricately linked with site quality factors. The estimated biomass of $1,995.98Mg{\cdot}ha^{-1}$ clearly indicates this. The site quality factor i.e. tropical moist deciduous with optimum availability of soil nutrients, heavy precipitation, high mean monthly relative humidity and optimum temperature range supported luxuriant growth which was realized as higher biomass accumulation and hence higher carbon accumulated.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1139-1146
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• 2007

Cultures of Saccharomyces cerevisiae were subjected to the effect of PEF (pulse electric field) and a source of selenium. The culture period after which yeast cells were subjected to PEF treatment was optimized, as was the duration of the exposure. Optimization of the nutrient medium composition in S. cerevisiae cultures resulted in an over 1.8-fold increase in selenium accumulation with relation to cultures on the initial substrate. Optimization of the pH value and of culture duration resulted in selenium accumulation increase by approximately 78%. A significant correlation was found between the accumulation of selenium in yeast cells and its concentration in the culture substrate. The highest accumulation of selenium in the biomass of yeast, approx. $240\;{\mu}g/g$ d.m., was obtained after 15-min exposure to PEF on a 20-h culture. An approx. 50% higher content of selenium in cells was recorded, as compared with the control culture without the application of PEF.

• Ecology and Resilient Infrastructure
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• 제10권4호
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• pp.161-170
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• 2023

본 연구에서는 제주도에서 자생하는 준맹그로브인 황근 (Hibiscus hamabo)과 갯대추나무 (Paliurus ramosissimus)의 탄소 저장량을 원격탐사로 추정하고 기후요인에 의하여 공간변이를 예측하는 인공지능 모델을 구축하고자 하였다. 준맹그로브의 지상부 탄소 축적량은 Global Ecosystem Dynamics Investigation (GEDI) 라이다에 의하여 제공되는 지상부 생물량 밀도(aboveground biomass density, AGBD)를 Sentinel-2 영상으로부터 추출한 normalized difference vegetation index (NDVI)으로 해상도를 상향하여 추정하였다. 제주도에서 단위면적당 탄소 축적량은 황근이 16.6 t C/ha, 갯대추나무가 21.1 t C/ha이었다. 제주도 전 해안에서 준맹그로브의 탄소 축적량은 11.5 t C로 추정되었다. 환경요인에 따른 준맹그로브의 탄소 축적량을 예측하기 위하여 랜덤 포레스트 기술을 적용하였다. 제주도 준맹그로브림의 분포면적 대비 지상부 생물량의 잔차를 계산하였다. 이 잔차에 영향을 미치는 주요 환경요인으로는 가장 습한 달의 강수량, 가장 더운 달의 최고온도, 등온성 및 가장 습한 달의 평균 온도가 선정되었다. 제주도에서 랜덤 포레스트 분석으로 예측된 준맹그로브의 탄소 축적량은 12.0 t C/ha - 27.6 t C/ha 범위의 공간적 변이를 나타내었다. 본 연구에서 개발된 탄소 축적량의 원격탐사 추정법과 환경요인에 따른 인공지능 예측법은 한반도에서 탄소흡수원으로서 맹그로브의 보전과 조성에 필요한 기초자료로 활용할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1920-1927
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• 2015

This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe²⁺, Mg²⁺, Ni²⁺, and Zn²⁺ further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe²⁺ addition.

• Journal of Plant Biotechnology
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• 제5권3호
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• pp.181-185
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• 2003

Callus and suspension cultures derived from leaf explants of Plumbago rosea were established on Murashige and Skoog's medium containing 1 mg/L IAA, 0.5 mg/L NAA and 0.3 mg/L BAP. Callus cultures were tested for their growth and accumulation of plumbagin, a naphthoquinone and was identified by $^1H$ NMR and electron ionization mass spectroscopy. While auxins (not 2,4-D) influenced growth and plumbagin accumulation, cytokinins did not influence them much. Increasing concentrations of IAA in presence of NAA and BAP increased plumbagin in suspensions only up to 1 mg/L. Growth of callus was optimum (8.3 g DCW/I) at a hormonal combination of 1.5 mg/L IAA, 0.5 mg/L NAA and 0.3 mg/L BAP, but high plumbagin accumulation (4.9 mg/g DCW) was recorded at 1.0 mg/L IAA plus 0.3 mg/L BAP. Since instability in growth and secondary metabolite accumulation was noticed, several cell lines/clumps of callus were screened for plumbagin accumulation by visual and analytical methods. Biomass and accumulation of plumbagin showed a negative correlation in several cell lines. But one cell line showed stability both in terms of biomass and plumbagin accumulation over a period of 6 months.

• 한국초지조사료학회지
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• 제39권3호
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• pp.148-152
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• 2019

Soil acidity or alkalinity are serious limitations for crop production. The purpose of this study was to clarify the negative effects of extreme pH stress (low and high) on alfalfa vegetative growth (VG) and biomass accumulation (BA). Two-week-old alfalfa seedlings were exposed to different pH (4.0, 4.5, 7.0, 8.0 and 8.5, respectively) levels for 72 hours. Alfalfa grown at pH 4.0 and 8.5 significantly reduced VG and BA, wherein as neutral pH (7.0) comparably exhibited better growth and biomass yield. These results indicate that extreme acidic or alkaline level are critical limiting factors for growth and biomass yield in alfalfa.

• Journal of Forest and Environmental Science
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• 제34권1호
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• pp.1-11
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• 2018

We studied natural and plantation forest ecosystem of Sarguja in Chhattisgarh, India in order to understand how vegetation biomass, carbon stock and its allocation patterns vary among the sites. For this, stratified random sampling was opted to measure the different layers of vegetation. Wide floral diversity was found in the natural forest site as compared to the teak stand. Overall, 17 tree species found in natural forest comprising 8 families while in the teak stand 6 species were recorded. In understory strata 23 species were recorded (18 herbs and 5 shrubs) in natural forest whereas in teak stand 20 herb species and 3 shrubs were found. Great variation was also seen in the population dynamics of the different vegetation stratum in concerned sites. The sapling, seedling and herb density was found to be highest in natural stand while tree and shrub density was more in teak stand. Results indicated that stand biomass of the natural site was $321.19t\;ha^{-1}$ while in the teak stand it was $276.61t\;ha^{-1}$. The total biomass of tree layer in plantation site was $245.22t\;ha^{-1}$ and natural forest $241.44t\;ha^{-1}$. The sapling, seedling, shrub and forest floor biomass was found highest under natural forest as compared to the teak plantation site. Carbon stock has similar trend as that of biomass accumulation in natural forest and teak stand. Higher biomass accumulation and carbon stock were recorded in the higher girth class gradation of the population structure. Proper efforts are required to manage these diverse ecosystems to obtain higher biomass and sustainable ecological services.