• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.2026-2026
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• 2019

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1566-1573
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• 2014

Although most algae cultivation systems are operated in suspended culture, an attached growth system can offer several advantages over suspended systems. Algal cultivation becomes light-limited as the microalgal concentration increases in the suspended system; on the other hand, sunlight penetrates deeper and stronger in attached systems owing to the more transparent water. Such higher availability of sunlight makes it possible to operate a raceway pond deeper than usual, resulting in a higher areal productivity. The attached system achieved 2.8-times higher biomass productivity and total lipid productivity of $9.1g\;m^{-2}day^{-1}$ and $1.9g\;m^{-2}day^{-1}$, respectively, than the suspended system. Biomass productivity can be further increased by optimization of the culture conditions. Moreover, algal biomass harvesting and dewatering were made simpler and cheaper in attached systems, because mesh-type substrates with attached microalgae were easily removed from the culture and the remaining treated wastewater could be discharged directly. When the algal biomass was dewatered using natural sunlight, the palmitic acid (C16:0) content increased by 16% compared with the freeze-drying method. There was no great difference in other fatty acid composition. Therefore, the attached system for algal cultivation is a promising cultivation system for mass biodiesel production.

• Molecules and Cells
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• v.37 no.1
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• pp.81-87
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• 2014

Chronic pressure-overload cardiac hypertrophy is associated with an increased risk of morbidity/mortality, largely due to maladaptive remodeling and dilatation that progresses to dilated cardiomyopathy. Alternative splicing is an important biological mechanism that generates proteomic complexity and diversity. The recent development of next-generation RNA sequencing has improved our understanding of the qualitative signatures associated with alternative splicing in various biological conditions. However, the role of alternative splicing in cardiac hypertrophy is yet unknown. The present study employed RNA-Seq and a bioinformatic approach to detect the RNA splicing regulatory elements involved in alternative splicing during pressure-overload cardiac hypertrophy. We found GC-rich exonic motifs that regulate intron retention in 5' UTRs and AT-rich exonic motifs that are involved in exclusion of the AT-rich elements that cause mRNA instability in 3' UTRs. We also identified motifs in the intronic regions involved in exon exclusion and inclusion, which predicted splicing factors that bind to these motifs. We found, through Western blotting, that the expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, were significantly altered during cardiac hypertrophy. Collectively, the present results suggest that chronic pressure-overload hypertrophy is closely associated with distinct alternative splicing due to altered expression of splicing factors.

• Journal of Biomedical Engineering Research
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• v.28 no.2
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• pp.195-198
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• 2007

In this paper, we introduce a digital cross-polarization and fluorescent color imaging system for quantitative evaluation of skin lesions. We describe the characterization of the imaging systems and the quantitative image analysis methods to show the feasibility for quantitative evaluation of skin lesions. The polarization color image was used to compute erythema and melanin index image which are useful for quantitative evaluation of pigmentation and vascular skin lesions, respectively. The fluorescent color image was used to quantitatively evaluate "sebum" and "vitiligo". In quantitative evaluation of various skin lesions, we confirmed the clinical efficacy of the imaging systems for dermatological applications. Finally, we sure that the imaging systems can be utilized as important assistant tools for the evaluation of skin lesions by providing reproducible quantitative result for widely distributed skin lesions.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.3 no.1
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• pp.1-9
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• 1999

Microzooplankton was analysed between $40^{\circ}s$ to $53^{\circ}s$S and $140^{\circ}E$ to $146^{\circ}E$ of the Sourthern Ocean from November 18 to November 30, 1995 to investigate the influence of frontal systems. The density and carbon biomass of microzooplankton were clearly associated with frontal systems, and at least 4 different communities were identified. The Subtropical Convergence Zone and Antarctic Polar Front Zone were the major biological boundaries recognized in the Southern Ocean. Ciliates predominated other microzooplankton in density and carbon biomass. Non-tintinnid ciliates occupied more than 70% of the total microzooplankton, and Laboea spp. was the major component of the non-tintinnid ciliates. The density and carbon biomass showed a decreasing tendency toward south from $40^{\circ}S$ to the $53^{\circ}S$ transect. The ecological importance of a frontal zone is confirmed by the microzooplanktonic data obtained from this study.

• Genomics & Informatics
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• v.1 no.2
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• pp.75-79
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• 2003

Every protein can be characterized by either a distinct motif or a combination of motifs. Nevertheless, little is known about the relationships among (more than two) the motifs. Some of the proteins in the world are share motifs for evolutional or other biological benefits - they can save energy, time and resource for controlling and managing a variety of proteins. In some cases of motifs, the tendency is quite common and they can act the 'hub' motif of a network of the motif associations. The hubs are structurally and functionally important in themselves and also important in disease-related mutations. They will be highly resistant mutation to conserve their functions. But, in case of the a rare mutation, mutations on the position of hub can more easily cause fatal diseases.

• Journal of Biomedical Engineering Research
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• v.18 no.2
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• pp.173-178
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• 1997

pH sensitive polymers have long been utilized as one important type among many interesting drug delivery systems. This is due to the reason of different pH environments in human organs, which requires different pH control mechanism depending upon the organs. In the present study the pH sensitivity was investigated for both pH sensitive and pH insensitive biopolymers using the diffusion effect along with the swelling effect. NMR microscopy was performed along with optical microscopy. For the analysis of diffusion effect, UMR Microscopy was perFormed to measure diffusion coefficients for various liquids such as distilled water, acetone and DMSO(dimethyl sulfoxide). Also, this technique is expected to contribute to the studies for many pH drug delivery systems.

• Advances in environmental research
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• v.5 no.3
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• pp.169-178
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• 2016

This study aimed at determining the treatability of high-strength wastewater (chemical oxygen demand, COD>4000 mg/L) using combined anaerobic-aerobic granular sludge in lagoon systems. The lagoon systems were simulated in laboratory-scale aerated and non-aerated batch processes inoculated with dried granular microorganisms at a dose of 0.4 g/L. In the anaerobic batch, a removal efficiency of 25% was not attained until the 12th day. It took 14 days of aerobic operation to achieve sCOD removal efficiency of 94% at COD:N:P of 100:4:1. The best removal efficiency of sCOD (96%) was achieved in the sequential anaerobic-aerobic batch of 12 days and 2 days, respectively at COD:N:P ratio of 200:4:1. Sequential anaerobic-aerobic treatment can achieve efficient and cost effective treatment for high-strength wastewater in lagoon systems.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.05
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• pp.18-21
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• 1996

Even though there are several deterministic methods for the modeling of linear systems, there is no standard method for the modeling of nonlinear systems. For the modeling of nonlinear systems we have applied the genetic programming method to estimate nonlinear time sereis. We get the time series from the simple known nonlinear dynamics, and fed those to genetic programming. For the tested nonlinear systems, suggested method estimated the nonlinear dynamics correctly.

• Journal of the Korean Magnetic Resonance Society
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• v.8 no.1
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• pp.55-61
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• 2004

Biosupramolecular systems such as biological membranes usually fluid under physiological conditions$^1$. Therefore, solid-state NMR has been used to investigate biosupramolecular systems. But solid-state NMR spectra contain a large number of overlapping resonances and are rather difficult to analyze. These problem has to be overcome by selective isotope labeling. We constructed a deuterium NMR probe for AM400 NMR spectrometer, which is mainly used for liquid samples. To overcome the fluidity problem, a saddle type coil was designed. The efficiency was systematically investigated for two kinds of coil geometry, solenoid and saddle types. Our results suggest that solenoids are superior to saddle type coils in the sensitivity. However, the letter fits better to fluid samples such as biosupramolecular systems.