• Transactions of the Korean hydrogen and new energy society
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• v.13 no.1
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• pp.3-12
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• 2002

In this study, it achieved life cycle assessment to estimate environmental performance for naphtha steam reforming that account for the production over 50% of total hydrogen output. Although hydrogen dosen't emit air emissions, especially, $CO_2$, a large of $CO_2$ is emitted in hydrogen production process. In the result of this study, it ascertained the truth that $CO_2$ is emitted at the rate of $6.3kg/kgH_2$ and that result from steam reforming reaction and use of fossil fuel in hydrogen manufacturing process. Above all, 57% of total $CO_2$ emissions is emitted in process of steam reforming of naphtha and so it knew that the principle of steam reforming is key issue in aspect to environment. Also, it compared hydrogen by fuel of fuel cell vehicle with gasoline fuel of general gasoline vehicle to analyze relative environment of hydrogen for fossil fuel during the life cycle. As the result, it might be difficult in improvement of environment because $CO_2$ emissions during the hydrogen manufacturing process is nearly the same with that during the use of gasoline.

• KSBB Journal
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• v.20 no.6
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• pp.464-469
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• 2005

For the biological production of hydrogen, a new fermentative hydrogen-producing bacterium, Enterobacter sp. SNU-1453, was isolated from a domestic landfill. During the culture of this bacterium, pH significantly decreased with the accumulation of various organic acids, and consequently this inhibited the production of hydrogen. It was found that the metabolic flux in this bacterium depended on the pH and affected the hydrogen production. A butanediol pathway was dominant during the fermentation when pH was not controlled. By controlling the pH at 7 this pathway can be shifted to a mixed acid pathway, which is favorable to the production of hydrogen.

• Journal of the Korean Ceramic Society
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• v.51 no.5
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• pp.477-480
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• 2014

An $Al_2O_3$-CuO-ZnO (ACZ) precursor powder was synthesized by a facial sol-gel process using a nonionic surfactant span 80 as the chelating agent to improve the surface area and morphology. When creating a hydrogen membrane, several kinds of properties are required, such as easy dissociation of hydrogen molecules, fast hydrogen diffusion, high hydrogen solubility, and resistance to hydrogen embrittlement. ACZ-Ni composite membranes (cermet) were prepared with this precursor and pure Ni powder via the hot press sintering (HPS) method. The ACZ powder was characterized by XRD, BET, and FE-SEM. Hydrogen permeation experiments were performed by Sievert's type of hydrogen permeation membrane equipment. The hydrogen permeability of ACZ/Ni 10 wt% and ACZ/Ni 20 wt% was obtained as 7.2 and $10molm^{-2}s^{-1}$ at RT, respectively. These values of the corresponding membranes were slightly increased with increasing pressures.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.240-244
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• 2003

A novel integrated method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide to the medium, which is then decomposed to oxygen and water by catalase and adding vegetable oil to the medium as antifoam agent and oxygen vector. A new apparatus for automated addition of hydrogen peroxide to the bioreactor to keep the dissolved oxygen concentration constant over the range $10-100%\;{\pm}\;5%$ was tested. A significant increase (over threefold) of cultivation time was obtained while the dissolved oxygen concentration remained stable ($30%\;{\pm}\;5%$). Therefore, use of corn oil mixed with Ca-stearate as oxygen vector and antifoam and hydrogen peroxide as oxygen source to control excessive foam that was generated by microorganism biosurfactant, GB16-BS produced at Bacillus sp. GB16 cultivation was appropriate for stable cultivation.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.445-453
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• 2007

The effect of an electrochemically generated oxidation-reduction potential and electric pulse on ethanol production and growth of Saccharomyces cerevisiae ATCC 26603 was experimented and compared with effects of electron mediators (neutral red, benzyl viologen, and thionine), chemical oxidants (hydrogen peroxide and hypochlorite), chemical reductants (sulfite and nitrite), oxygen, and hydrogen. The oxidation (anodic) and reduction (cathodic) potential and electric pulse activated ethanol production and growth, and changed the total soluble protein pattern of the test strain. Neutral red electrochemically reduced activated ethanol production and growth of the test strain, but benzyl viologen and thionine did not. Nitrite inhibited ethanol production but did not influence growth of the test strain. Hydrogen peroxide, hypochlorite, and sulfite did not influence ethanol production and growth of the test strain. Hydrogen and oxygen also did not influence the growth and ethanol production. It shows that the test strain may perceive electrochemically generated oxidation-reduction potential and electric pulse as an environmental factor.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.412-417
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• 2001

Sodium alginate was used to immobilize Bifidobacterium breve ATCC 15700 cells. The ability of the Ca-alginate beads to protect the B. breve ATCC 15700 was evaluated under different conditions including alginate concentration, bead size, pH, hydrogen peroxide, and storage period. The survival of the B. Breve ATCC 15700 was estimated in pasteurized yogurt, containing either the immobilized or free cells, throughout the storage period. The survival cells in bead after exposure to acidic solution (pH 3.0) increased with increase of both the alginate gel concentration and bead size. Also, immobilized cells in alginate bead were more resistant than the free cells to hydrogen peroxide, storage period, and the environment inside yogur. When retreated beads with skim milk and nonretreated beads were tested in acidified pH 3.0 TPY media including acetic and lactic acid, the number of viable cells in the retreated bead was approximately 10-fold higher than that of nonretreated beads. This suggests that the skim milk operated as a material decreasing the diffusion of acid and hydrogen perosicde into alginate gels. From this research, it was found that yogurt itself supported immobilized cells with an improved protection from the extreme acidity in yogurt.

• Journal of Microbiology and Biotechnology
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• v.10 no.1
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• pp.35-42
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• 2000

Reaction characteristics of 4-methylcatechol 2,3-dioxygenase (4MC230) purified from Pseudomonas putida SU10 with a higher activity toward 4-methylcatechol than catechol or 3-cethylcatechol were studied by altering their physical and chemical properties. The enzyme exhibited a maximum activity at pH 7.5 and approximately 40% at pH 6.0 for 4-methylcatechol hydrolysis. The optimum temperature for the enzyme was around $35^{\circ}C$, since the enzyme was unstable at higher temperature. Acetone(10%) stabilized the 4MC230. The effects of solvent and other chemicals (inactivator or reactivator) for the reactivation of the 4MC230 were also investigated. Silver nitrate and hydrogen peroxid severely deactivated the enzyme and the deactivation by hydrogen peroxide severely deactivated the enzyme and the deactivation by hydrogen peroxide was mainly due to the oxidation of ferrous ion to ferric ion. Some solvents acted as an activator and protector for the enzyme from deactivation by hydrogen peroxide. Ascorbate, cysteine, or ferrous ion reactivated the deactivated enzyme by hydrogen peroxide. The addition of ferrous ion together with a reducing agent fully recovered the enzyme activity and increased its activity abut 2 times.

• Korean Chemical Engineering Research
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• v.46 no.3
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• pp.598-603
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• 2008

In petrochemical complex, Hydrogen Network optimization is surveying the extra Hydrogen and needed Hydrogen of each company and calculating the optimized distribution. This paper compares the case of using the existing pipeline and the case of not using the existing pipeline to show the effect quantitatively and clearly by modeling the both cases and using mathematical programming. As a result, using the existing pipeline can save the pipe cost over 20% and increase the whole network benefit by thirteen billion won.

• Proceedings of the Microbiological Society of Korea Conference
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• 2004.05a
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• pp.101-104
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• 2004

Among biological hydrogen production processes, fermentative processes have some advantages. In this research, the hydrogen producing bacterium was isolated from domestic landfill area and identified as Enterobacter sp. The strain was named Enterobacter sp. SNU-1453. Important parameters for the hydrogen process include pH, temperature, concentration of initial glucose, and kind of sugars. The pH of the culture medium significantly decreased as fermentation proceeded due to the accumulation of various organic acids, and this inhibited the $H_2$ production seriously. When pH was controlled at pH 7.0, hydrogen production was 2614.5 m1/1 in 17 hours. The increase of glucose concentration resulted in higher $H_2$ production. The productivity of this strain was 6.87 mmol $H_2/l$ per hi on concentration of 25g glucose/l. Enterobacter sp. SNU-1453 could utilize various sugars. These results indicate that Enterobacter sp. SNU-1453 has a high potential as a fermentative $H_2$ producer.

• Korean Chemical Engineering Research
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• v.45 no.4
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• pp.372-377
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• 2007

In order to assess its capability as biofilter bed material under variable conditions of two parameters (inlet gas concentration and inlet gas flow rate), reticulated polyurethan was applied to remove hydrogen sulfide via a biological process. We detected a maximal elimination capacity (critical loading rate) of $488.3(330.1)g-H_2S/m^3{\cdot}hr$, when reticulated polyurethane was employed as supporting material of biofilter. This study show that the application of reticulated polyurethane carrier might be a favorable choice as a packing material in biofilter for the biological removal of hydrogen sulfide.