• Weed & Turfgrass Science
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• 제4권2호
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• pp.135-143
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• 2015

Typhula ishikariensis Imai is a causal agent of Typhula snow mold, one of the most important turfgrass diseases in northern regions of the United States. Within Wisconsin isolates, there are three district groups clustered with known isolates of T. ishikariensis var. ishikariensis, var. canadensis and var. idahoensis as identified by RAPD markers. To further investigate the genetic relationship among these groups (varieties), monokaryon-monokaryon and dikaryon-monokaryon mating experiments were conducted. Mating types from var. ishikariensis, var. canadensis and var. idahoensis isolates were paired in all possible combinations. Pairings between var. canadensis and var. idahoensis were highly compatible, while no compatibility was detected between var. ishikariensis and either var. canadensis or var. idahoensis. These results indicate that var. ishikariensis is genetically separated from var. canadensis and var. idahoensis, whereas var. canadensis and var. idahoensis appeared to be genetically related to each other as a taxonomic unit. In the genetic relationship with the known biological species, var. ishikariensis and var. canadensis were genetically related to biological species I and II, respectively. However, var. idahoensis was not compatible with any of the biological species, suggesting that the pathogen may be in the process of biological speciation from var. canadensis.

• 공업화학
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• 제22권1호
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• pp.45-47
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• 2011

우수한 항균성능을 가지는 것으로 알려져 있는 N-halamine에 알킬기를 도입하여 페인트용 항균성 물질인 Dichlorohexyl isocyanuric acid (DCHICA)를 합성하였다. 그램 음성균인 E. coli를 사용한 항균테스트, 곰팡이를 이용한 antifungal 테스트를 진행하여 우수한 항균성을 확인하였고, 페인트와의 혼합농도, 시간에 따른 항균성 역시 관찰하였다. 또한, 도입된 알킬기로 인해 도포시에도 우수한 표면항균활성을 가지는 것을 확인하였다.

• 한국토양비료학회지
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• 제50권4호
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• pp.226-234
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• 2017

In order to reduce the accumulation of toxic metals (As, Cd and Pb) in the chives, various immobilizing agents such as a soil pH change-inducing immobilizing agent (lime), sorption agent (compost, spent mushroom compost), soil pH change and sorption agent (biochar) and, dissolved organic carbon (DOC) coagulator (gypsum) and uncontaminated soil were applied to the contaminated soils in isolation and in combination. Then chives were grown and determined for As, Cd and Pb concentrations accumulated in the edible part at harvest. The Cd and Pb concentrations of the chive plant grown in the contaminated soil (no treatment) exceeded the legislated Korean guideline values (Cd: $0.05mg\;kg^{-1}$, Pb $0.1mg\;kg^{-1}$) and As concentration ($21mg\;kg^{-1}$) was 1,000 times higher than chives plant grown in uncontaminated environment in Korea. Application of lime and gypsum significantly reduced As, Cd and Pb concentrations in all chives examined, due to the increased soil pH and decreased soil DOC. Also, application of combination treatments involving DOC coagulator such as gypsum together with lime decreased As, Cd and Pb concentrations from 21, 1.3 and $9.7mg\;kg^{-1}$ to 2.1, 0.1 and $1.1mg\;kg^{-1}$, respectively. Consequently, it was concluded that pH change-inducing immobilizing agent (lime) which was already well known and DOC coagulator such as gypsum could be used as a promising immobilizing agent for safer chives plant production.

• 한국연초학회지
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• 제27권2호
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• pp.212-218
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• 2005

Cigarette smoking has been known to have a few beneficial effects on some neuronal diseases such as Alzheimer's disease(AD), Parkinson's disease(PD) and prion disease by scrapie agent shows many similar properties with AD. In this respect, we investigated what biological effects are exerted by cigarette smoke exposure(CSE) in the brain of mouse infected by 87V scrapie. The scrapie agent was inoculated through stereotaxic microinjection of the homogenates of the scrapie agent infected brain into the intracerebral system in the 1M mice. The inoculation into mice typically exhibits neurochemical, physiological and histopathological characteristics of prion disease: loss of neurotransmitters and induction of astrocytosis and vacuolation in brain as well as reduction of spatial movement and loss of body weight. CSE led to alleviated the loss of body weight and also improved spatial movement of the infected mice. Most interestingly, CSE attenuated astrocytosis and vacuolation caused by scrapie infection in the brain. In addition, decreased levels of dopamine in striatal and hypothalamic regions as well as serotonin level in hippocampus caused by scrapie infection were also attenuated by exposure to cigarette smoke. These findings suggest that cigarette smoke, by its inhibition of astrocytosis and vacuolation followed by its restoration of levels of some neurotransmitters, may partly contribute to suppression in the progress of neurodegeneration caused by scrapie infection.

• 한국정밀공학회지
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• 제29권4호
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• pp.373-379
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• 2012

Disruptions in manufacturing systems caused by system changes and disturbances such as the tool wear, machine breakdown, malfunction of transporter, and so on, reduce the productivity and the increase of downtime and manufacturing cost. In order to cope with these challenges, a new method to build an intelligent manufacturing system with biological principles, namely an ant colony inspired manufacturing system, is presented. In the developed system, the manufacturing system is considered as a swarm of cognitive agents where work-pieces, machines and transporters are controlled by the corresponding cognitive agent. The system reacts to disturbances autonomously based on the algorithm of each autonomous entity or the cooperation with them. To develop the ant colony inspired manufacturing system, the disturbances happened in the machining shop of a transmission case were analyzed to classify them and to find out the corresponding management methods. The system architecture with the autonomous characteristics was generated with the cognitive agent and the ant colony technology. A test bed was implemented to prove the functionality of the developed system.

• BMB Reports
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• 제51권10호
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• pp.526-531
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• 2018

Protein-Protein Interactions (PPIs) play essential roles in diverse biological processes and their misregulations are associated with a wide range of diseases. Especially, the growing attention to PPIs as a new class of therapeutic target is increasing the need for an efficient method of cell-based PPI analysis. Thus, we newly developed a robust PPI assay (SeePPI) based on the co-translocation of interacting proteins to the discrete subcellular compartment 'processing body' (p-body) inside living cells, enabling a facile analysis of PPI by the enriched fluorescent signal. The feasibility and strength of SeePPI (${\underline{S}}ignal$ ${\underline{e}}nhancement$ ${\underline{e}}xclusively$ on ${\underline{P}}-body$ for ${\underline{P}}rotein-protein$ ${\underline{I}}nteraction$) assay was firmly demonstrated with FKBP12/FRB interaction induced by rapamycin within seconds in real-time analysis of living cells, indicating its recapitulation of physiological PPI dynamics. In addition, we applied p53/MDM2 interaction and its dissociation by Nutlin-3 to SeePPI assay and further confirmed that SeePPI was quantitative and well reflected the endogenous PPI. Our SeePPI assay will provide another useful tool to achieve an efficient analysis of PPIs and their modulators in cells.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.87-92
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• 2014

The work is directed toward the synthesis of a series of DO3A conjugates of tranexamates (1c-e) and their Gd complexes (2c-e) for use as a liver-specific MRI CA. All these complexes show thermodynamic and kinetic stabilities comparable to those of structurally related clinical agents such as Dotarem$^{(R)}$. Their $R_1$ relaxivities also compare well with those of commercial agent, ranging 3.68-4.84 $mM^{-1}s^{-1}$. In vivo MR images of mice with 2a-e reveal that only 2a exhibits liver-specificity. Although 2b and 2c show strong enhancement in liver, yet no bile-excretion is observed to be termed as a liver-specific agent. The rest behaves much like ordinary ECF CAs like Dotarem$^{(R)}$. The new series possess no toxicity to be employed in vivo.

• Bulletin of the Korean Chemical Society
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• 제29권3호
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• pp.595-598
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• 2008

Emodin (3-methyl-1,6,8-trihydroxyanthraquinone) is a natural chemotherapeutic compound with diverse biological properties including an antitumor activity. Emodin, a specific inhibitor of the protein tyrosine kinase, has a number of cellular targets in related to it. Its inhibition activity affects the mammalian cell cycle regulation in specific oncogene. Practically, it has been proven to inhibit HER-2/neu tyrosine kinase expressing breast cancer cells as an anticancer agent. 2-[123I]iodoemodin has been synthesized and evaluated human breast cancer cells (MDA-MB-231, MCF-7, fibroblast as a control) which express basal levels of HER-2/neu tyrosine kinase to investigate its suitability as a breast cancer imaging agent and 2-iodoemodin has been synthesized as a standard compound. The radiochemical yield of the 2-[123I]iodoemodin was about 72% and its radiochemical purity was over 97% after purification. The radioactivity of the 2-[123I]iodoemodin was increased in a time dependent manner in both cell lines and the ratio of MDA-MB-231 and MCF7 to fibroblast was 2.9 and 1.7, respectively.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.573-583
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• 2017

Biofilm formation on the membrane surface results in the loss of permeability in membrane bioreactors (MBRs) for wastewater treatment. Studies have revealed that cellulose is not only produced by a number of bacterial species but also plays a key role during formation of their biofilm. Hence, in this study, cellulase was introduced to a MBR as a cellulose-induced biofilm control strategy. For practical application of cellulase to MBR, a cellulolytic (i.e., cellulase-producing) bacterium, Undibacterium sp. DM-1, was isolated from a lab-scale MBR for wastewater treatment. Prior to its application to MBR, it was confirmed that the cell-free supernatant of DM-1 was capable of inhibiting biofilm formation and of detaching the mature biofilm of activated sludge and cellulose-producing bacteria. This suggested that cellulase could be an effective anti-biofouling agent for MBRs used in wastewater treatment. Undibacterium sp. DM-1-entrapping beads (i.e., cellulolytic-beads) were applied to a continuous MBR to mitigate membrane biofouling 2.2-fold, compared with an MBR with vacant-beads as a control. Subsequent analysis of the cellulose content in the biofilm formed on the membrane surface revealed that this mitigation was associated with an approximately 30% reduction in cellulose by cellulolytic-beads in MBR.

• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.280-285
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• 2006

Methanol extract of the rhizomes of turmeric, Curcuma longa L., effectively controlled the development of red pepper anthracnose caused by Colletotrichum coccodes. In addition three antifungal substances were identified from the methanol extract of C. longa rhizomes as curcumin, demethoxycurcumin, and bisdemethoxycurcumin using mass and $^{1}H-NMR$ spectral analyses. The curcuminoids in a range $0.4-100\;{\mu}g/ml$ effectively inhibited the mycelial growth of three red pepper anthracnose pathogens, C. coccodes, C. gloeosporioides, and C. acutatum. The three curcuminoids inhibited mycelial growth of C. coccodes and C. gloeosporioides to an extent similar to the synthetic fungicide dithianon did, but the synthetic agent was a little more effective against C. acutatum. The curcuminoids also effectively inhibited spore germination of C. coccodes, and bisdemethoxycurcumin was the most active. Among the three curcuminoids, only demethoxycurcumin was effective in a greenhouse test in suppressing red pepper anthracnose caused by C. coccodes.