Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mitigation of Membrane Biofouling in MBR Using a Cellulolytic Bacterium, Undibacterium sp. DM-1, Isolated from Activated Sludge

  • Nahm, Chang Hyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Seonki (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Sang Hyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Kibaek (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Jaewoo (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kwon, Hyeokpil (School of Chemical and Biological Engineering, Seoul National University) ;
  • Choo, Kwang-Ho (Department of Environmental Engineering, Kyungpook National University) ;
  • Lee, Jung-Kee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Jang, Jae Young (Pure Envitech Co., Ltd.) ;
  • Lee, Chung-Hak (School of Chemical and Biological Engineering, Seoul National University) ;
  • Park, Pyung-Kyu (Department of Environmental Engineering, Yonsei University)
  • Received : 2016.10.05
  • Accepted : 2017.01.03
  • Published : 2017.03.28
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Abstract

Biofilm formation on the membrane surface results in the loss of permeability in membrane bioreactors (MBRs) for wastewater treatment. Studies have revealed that cellulose is not only produced by a number of bacterial species but also plays a key role during formation of their biofilm. Hence, in this study, cellulase was introduced to a MBR as a cellulose-induced biofilm control strategy. For practical application of cellulase to MBR, a cellulolytic (i.e., cellulase-producing) bacterium, Undibacterium sp. DM-1, was isolated from a lab-scale MBR for wastewater treatment. Prior to its application to MBR, it was confirmed that the cell-free supernatant of DM-1 was capable of inhibiting biofilm formation and of detaching the mature biofilm of activated sludge and cellulose-producing bacteria. This suggested that cellulase could be an effective anti-biofouling agent for MBRs used in wastewater treatment. Undibacterium sp. DM-1-entrapping beads (i.e., cellulolytic-beads) were applied to a continuous MBR to mitigate membrane biofouling 2.2-fold, compared with an MBR with vacant-beads as a control. Subsequent analysis of the cellulose content in the biofilm formed on the membrane surface revealed that this mitigation was associated with an approximately 30% reduction in cellulose by cellulolytic-beads in MBR.

Keywords

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