• Title/Summary/Keyword: Biological agent

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Effects of Silicone Surfactant on the Cell Size and Thermal Conductivity of Rigid Polyurethane Foams by Environmentally Friendly Blowing Agents

  • Han, Mi-Sun;Choi, Seok-Jin;Kim, Ji-Mun;Kim, Youn-Hee;Kim, Woo-Nyon;Lee, Heon-Sang;Sung, Joon-Yong
    • Macromolecular Research
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    • v.17 no.1
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    • pp.44-50
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    • 2009
  • Rigid polyurethane foams (PUF)s were synthesized with environmentally friendly blowing agents such as a cyclopentane/distilled water (10.0/1.0, pphp) mixture and distilled water only for four different silicone surfactants having different silicone/polyether ratios. An attempt was made to reduce the thermal conductivities of the PUF samples by varying the concentration and the silicone/polyether ratio of the various silicone surfactants. The scanning electron microscopy (SEM) results indicated an optimum concentration of the silicone surfactant of about 1.5 to 2.5 phpp for various surfactants to reduce the cell size and lower the thermal conductivity. The silicone surfactant having a higher silicone/polymer ratio showed a smaller cell size and, therefore, demonstrated the lower thermal conductivity of the PUF samples. From the relation between the thermal conductivity and the cell size of the PUF samples, the smaller cell size improved the thermal insulation property of the rigid PUF for both the PUF samples blown by the cyclopentane/distilled water (10.0/1.0, pphp) mixture and distilled water only. If the blowing agent is fixed, then the cell size is an important factor to decrease the thermal conductivity of the PUF samples. These results indicated that rigid PUF samples having lower thermal conductivity can be obtained by choosing a silicone surfactant containing a higher silicone/polyether ratio, as well as an optimum content of the surfactant.

Effects of Nucleating Agents on the Morphological, Mechanical and Thermal Insulating Properties of Rigid Polyurethane Foams

  • Kang, Ji-Woung;Kim, Ji-Mun;Kim, Min-Soo;Kim, Youn-Hee;Kim, Woo-Nyon;Jang, Won;Shin, Dae-Sig
    • Macromolecular Research
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    • v.17 no.11
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    • pp.856-862
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    • 2009
  • This study examined the effects of liquid and solid additives on the morphological, mechanical and thermal insulating properties of rigid polyurethane foams (PUFs). The PUFs synthesized with tetramethylsilane (TEMS) as a liquid-type additive showed a smaller average cell size and lower thermal conductivity than those with the aerosil 200 and clay 30B as solid-type additives. When TEMS was added, the average cell size of the PUF became more uniform and finer due to the reduced surface tension of the polymer solution, which increased the nucleation rate and number of bubbles produced and reduced cell size. The PUFs with TEMS showed the highest closed cell contents among the PUFs prepared using TEMS, aerosil 200 and clay 30B. This suggests that the insulation properties of PUF can be determined by both the size of the cell structure and the amount of closed cell contents in the system. The compression and flexural strengths of the PUF increased slightly when the aerosil 200, clay 30B and TEMS were added compared those of the neat PUF. The reaction profiles of the PUFs showed a similar gel and tack tree time with the reaction time among the PUFs synthesized with three different additives and neat PUF. This suggests that the nucleating additives used in this study do not affect the bubble growth of the chemical reaction, and the additives may act as nucleating agents during the formation of PUF. From the above results of the cell size, thermal conductivity, closed cell contents and reaction profile of the PUFs, liquid-type nucleating agent, such as TEMS, is more effective in decreasing the thermal conductivity of the PUF than solid-type nucleating agent, such as aerosil 200 and clay 30B.

Weed Management Technology with Host Specific of Biological Control Agents (기주특이성 잡초 활성 미생물을 이용한 잡초방제 기술)

  • Hong, Yeon-Kyu;Lee, Bong-Choon;Song, Seok-Bo;Park, Sung-Tae;Kim, Jeong-Nam;Geon, Min-Goo;Kim, In-Seob
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.175-190
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    • 2006
  • The term mycoherbicide started in 1970, but its interest heightened due to increase costs of chemical herbicides. A classical biocontrol agent is expected to become a permanent part of its new environment and do no harm to it. Contemporary biological control agent(BCA) must be produced by artificial culture and could be applied like chemical herbicides over weeds. BCA is different from the classical approach in that it released through natural spread. To date 26 species of fungi are used as classical BCA against 26 species of weeds in seven countries. There are a number of examples of pathogens attacking non-target plants. But through risk assessments which include understanding the taxonomy, biology and ecology, the target and non-target species, it will be safe to introduce of exotic pathogens to control weeds. But pathogens have not been successfully used in practice. Many mycoherbicides show potential in laboratories, but are ineffective in the field and not consistent from year to year or field to field. There is also a lack of understanding humidity, dew formation and temperature and their effects on suppression of weeds by plant pathogens. Potential pathogen must be selected as a BCA. Previous studies suggest that these pathogens must (1) produce abundant and durable inoculum in artifical culture, (2) be genetically stable and weed specific and (3) kill weeds in control. A granular preparation of mycoherbicide into sodium alginate is lighter than liquids and less bulky than organic matter. Gel forms have also been used.

Synthesis of Oxazolidinone Phosphonate Derivatives, Part I

  • Hwang, Jae-Min;Shin, Woon-Seob;Jung, Kang-Yeoun
    • Bulletin of the Korean Chemical Society
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    • v.25 no.9
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    • pp.1326-1330
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    • 2004
  • Several new derivatives of oxazolidinone phosphonate, that are expected to show biological activity, were prepared efficiently by previously published methods. ${\gamma}$-Ketophosphonate 1 was synthesized using pentacovalent oxaphosphorane chemistry followed by reductive amination with aromatic amine of oxazolidinone 4. Biological activity of the synthetic compounds has been studied. One of the synthetic compounds showed promising result for us to pursue further studies.

Isolation of Bacillus licheniformis Producing Antimicrobial Agents against Bacillus cereus and Its Properties (Bacillus cereus 증식 억제능을 가지는 Bacillus licheniformis SCK 121057의 분리 및 특징)

  • Kim, Yong-Sang;Yun, Suk-Hyun;Jeong, Do-Yeon;Hahn, Kum-Su;Uhm, Tai-Boong
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.270-277
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    • 2010
  • In order to manufacture Bacillus cereus-free fermented soybean products, an antimicrobial agentproducing isolate against B. cereus was obtained from 150 traditionally fermented soybean products. The morphological and biochemical tests and the phylogenetic relationship among 16S rRNA gene sequences indicated that the isolate named as the strain SCK 121057 was most closely related to Bacillus licheniformis. The B. licheniformis isolate began to produce the antimicrobial agent after 48 h of incubation. The agent was nonproteinaceous and insensitive to heat, long term storage and protease K. Electron microscopic observation indicated that the agent attacked the membrane of B. cereus, leaving the ghost cell. The isolate inhibited growth of B. subtilis, Lactobacillus brevis and various types of pathogenic strains including Escherichia coli, E. faecalis, Micrococcus luteus, Staphylococcus aureus, Aspergillus flavus, A. ochraceus, and A. parasiticus as well as B. cereus. After coinoculation of B. licheniformis SCK 121057 and B. cereus in the ratio (as the basis of CFU/g sample) of 10 to 1 on the surface of cooked soybeans, cell numbers of B. cereus had been dramatically reduced after 31 days of incubation compared to those of single inoculation of B. cereus.

Effective Screening of Antagonist for the Biological Control of Soilborne Infectious Disease (Damping-Off)

  • LEE BAEK-SEOK;LEE HYANG-BOK;CHOI SUNG-WON;YUN HYUN-SHIK;KIM EUN-KI
    • Journal of Microbiology and Biotechnology
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    • v.15 no.4
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    • pp.701-709
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    • 2005
  • An efficient method of selecting an antagonistic strain for use as a biological control agent strain was developed. In this improved method, the surface tension reduction potential of an isolate was included in the 'decision factor,' in addition to two other factors; the growth rate and pathogen inhibition. By using a statistically designed method, an isolate from the soil was selected and identified as Bacillus sp. GB 16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth were observed when the Bacillus sp. GB 16 was used. The action of the surface tension reducing component was assumed to enhance the wetting, spreading, and residing of the antagonistic strain in the rhizosphere. This result showed that the improved selection method was quite effective in selecting the best antagonistic strain for the biological control of soilborne infectious plant pathogens.

Melanogenesis Inhibitory Activity of Epicatechin-3-O-Gallate Isolated from Polygonum amphibium L.

  • Lee, Young Kyung;Hwang, Buyng Su;Hwang, Yong;Lee, Seung Young;Oh, Young Taek;Kim, Chul Hwan;Nam, Hyeon Ju;Jeong, Yong Tae
    • Microbiology and Biotechnology Letters
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    • v.49 no.1
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    • pp.24-31
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    • 2021
  • This study aimed to investigate the melanogenesis inhibitory activity of epicatechin-3-O-gallate (ECG) isolated from Polygonum amphibium L. ECG was isolated from the ethanol extract of P. amphibium L, and its chemical structure was determined using spectroscopic methods such as LC-ESI-MS, 1D-NMR, and UV spectroscopy. ECG inhibited the melanogenesis of B16F10 cells in a dose-dependent manner. Particularly, it decreased the melanin content by 27.4% at 200 µM concentration, compared with the control, in B16F10 cells, without causing cytotoxicity. It is noteworthy that the expression of three key proteins, including tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF), involved in melanogenesis, is significantly inhibited by ECG. The ECG isolated in this study caused the inhibition of body pigmentation and tyrosinase activity in vivo in the zebrafish model. These results suggest that the ECG isolated from P. amphibium L. is an effective anti-melanogenesis agent.

The Relationship among Sun-Screening Agent Use, Bone Health Promotion Behavior and Bone Mineral Density of Female College Students (여대생의 자외선 차단제 사용과 골 건강증진행위 및 골밀도와의 관계)

  • Hyun, Hye Jin;Kim, Joo Hyun;Ko, Ga Yeon;Park, Bock Soon;Choi, Eun Young;Ahn, Mi Hyang
    • Journal of Korean Biological Nursing Science
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    • v.15 no.4
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    • pp.202-209
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    • 2013
  • Purpose: The purpose of this study is to investigate relationships among sun-screening agents use, bone health promotion behavior, and bone mineral density. Methods: The participants of this study were 105 female college students. The data were collected using a questionnaire about sun-screening agent use and bone health promotion behavior. Bone mineral density was measured with Ultrasound Bone Densitometer/Osteo Pro Series. Data were analyzed with the SPSS/Win 18.0 program. Results: The mean Z-score in female college students was $-1.04{\pm}1.26$, and the T-score was $-1.16{\pm}1.22$, 21.39% of the participants have osteopenia. Bone mineral density showed a significant correlation with weight. Bone mineral density showed no significant correlation between sun-screening agent use, and bone health promotion behavior. There was a significant correlation between protection power of sun-screening agents and the protection by clothes. Conclusion: According to the results of this study, we need to develop an intervention program for bone health promotion of female college students.

Fermentation of a Potential Biocontrol Agent, Bacillus amyloliquefaciens SKU-78 Strain (풋마름병균의 길항세균 Bacillus amyloliquefaciens SKU-78의 대량 배양 조건 확립)

  • Kim, Shin-Duk;Cho, Hong-Bum
    • Korean Journal of Microbiology
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    • v.50 no.1
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    • pp.84-86
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    • 2014
  • Mass production of biocontrol agent is an essential step for its commercial use. Media composition and culture conditions for production of Bacillus amyloliquefaciens SKU-78, a potential biocontrol agent against bacterial wilts, were optimized by a flask culture. Low cost media combining nitrogen and carbon sources were tested. Maximum cell growth (> $2{\times}10^9$ CFU/ml) was obtained in a medium of 5% soy flour combined with 3% corn starch after 24 h cultivation. The optimum initial pH, temperature and shaking speed was 5.5, $30^{\circ}C$ and 150-250 rpm, respectively. Fermentation of SKU-78 was scaled up in 30 L fermenter and the profiles of cell density, pH, dissolved oxygen and spore formation were recorded. After 8 h lag phase, exponential growth occurred and reached at maximum viable cell number ($1.2{\times}10^{11}$ CFU/ml) after 20 h. The SKU-78 strain grown in a low cost medium exhibited the high suppression of bacterial wilts. The results indicate that SKU-78 strain can be produced in a low cost medium and provide a basis for scaling up to industrial level.