• Title/Summary/Keyword: Biological Resources

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Identification and Characterization of an Agarase- and Xylanse-producing Catenovulum jejuensis A28-5 from Coastal Seawater of Jeju Island, Korea (제주 연안해수로부터 한천 분해 효소 및 자일란 분해 효소를 생산하는 Catenovulum jejuensis A28-5의 동정 및 특성 규명)

  • Kim, Da Som;Jeong, Ga Ram;Bae, Chang Hwan;Yeo, Joo-Hong;Chi, Won-Jae
    • Microbiology and Biotechnology Letters
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    • v.45 no.2
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    • pp.168-177
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    • 2017
  • Strain A28-5, which can degrade xylan and agar in solid medium, was isolated from a coastal seawater sample collected from Jeju Island, South Korea. This strain was found to be a gram-negative, $Na^+$-requiring bacterial strain with a polar flagellum for motility. Additionally, the strain was tolerant to antibiotics such as ampicillin and thiostrepton. The G+C content of the genome was 43.96% and menaquinone-7 was found to be the predominant quinone. Major fatty acids constituting the cell wall of the strain were $C_{16:1}$ ${\omega}7c/iso-C_{15:0}$ 2-OH (23.32%), $C_{16:0}$ (21.83%), and $C_{18:1}$ ${\omega}7c$ (17.98%). The 16S rRNA gene sequence of the strain showed the highest similarity (98.94%) to that of Catenovulum agarivorans YM01, which was demonstrated by constructing a neighbor-joining phylogenetic tree. A28-5 was identified as a novel species of the genus Catenovulum via DNA-DNA hybridization with Catenovulum agarivorans YM01, and thus was named as Catenovulum jejuensis A28-5. The formation of tetramers and hexamers of xylooligosaccharides and (neo)agarooligosaccharides, respectively, were confirmed by thin-layer chromatography analysis using an enzyme reaction solution containing xylan or agarose with two crude enzymes prepared from the liquid culture of the strain.

Antioxidant Activity of 11 Species in Korean Native Forest Plants (국내 산림식물 11종의 항산화 활성)

  • Cho, MyoungLae;Lee, Jong Seok;Lee, Sarah;Son, Youn Kyoung;Bae, Chang-Hwan;Yeo, Joohong;Lee, Hae-Sock;Ma, Jin-Gyeong;Lee, Ok-Hwan;Kim, Jong-Yae
    • The Korean Journal of Food And Nutrition
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    • v.28 no.6
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    • pp.1098-1106
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    • 2015
  • This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

A Floristic Study of Baengnyeongdo (Isl.) in Korea (백령도 지역의 관속식물상)

  • Kim, Jung-Hyun;Nam, Gi-Heum;Kim, Sun-Yu;Kim, Jin-Seok;Choi, Ji-Eun;Lee, Byoung Yoon
    • Korean Journal of Plant Resources
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    • v.26 no.2
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    • pp.178-213
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    • 2013
  • This study was carried out to investigate the flora of Baengnyeongdo (Isl.). The vascular plants which were collected in 5 times (from Aug. 2011 and Nov. 2012) were identified as 732 taxa in total, including 124 families, 411 genera, 647 species, 8 subspecies, 68 varieties, 7 forms and 2 hybrids. 193 of these taxa were first records for the region. In the flora of this area, the endangered plants designated and protected by the wildlife protection law, the Ministry of Environment, Korea were counted to two taxa. Korean endemics plants numbered 8 taxa, and 78 taxa of specially designated floristic regional indicator plants by the Ministry of Environment. And rare plants were identified as 20 taxa. Based on the list of southern and northern limit of distribution plants, 30 taxa and 43 taxa of halophytes were recorded in the investigated area. The naturalized plants were identified as 92 taxa, and their naturalization ratio were found to be 12.5%, respectively.

Development of microsatellite markers to assess the genetic diversity of the red-tongue viper, Gloydius ussuriensis (Reptilia: Viperidae) on the Korean Peninsula

  • Jung A Kim;Mu-Yeong Lee;Hye Sook Jeon;Min Seock Do;Kyo Soung Koo;Sang-Cheol Lee;Ji-Hwa Jung;Yoon-Jee Hong;Junghwa An
    • Journal of Species Research
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    • v.12 no.4
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    • pp.281-285
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    • 2023
  • The red-tongue viper(Gloydius ussuriensis) is one of only three species of the genus Gloydius found in South Korea. Gloydius ussuriensis has a narrow activity radius and is distributed nationwide, and this species was reported to have the largest population among the Korean species in genus Gloydius. Preliminary results of a phylogenetic analysis using part of the mitochondrial DNA indicated that domestic G. ussuriensis is not comprised of monophyletic groups, and morphological analysis showed differences between domestic populations. In this study, we developed 17 microsatellites for the analysis of G. ussuriensis genetic diversity based on these characteristics. These microsatellites were developed using six multiplex panels, which could be employed to validate 80 G. ussuriensis specimens from different geographical regions in South Korea. The average number of alleles per locus was 12.2 and ranged from 4 to 25 alleles; the observed heterozygosity ranged from 0.238 to 0.950 and the expected heterozygosity ranged from 0.213 to 0.933. As a result of assessing four inland populations, a high level of genetic diversity was confirmed. These newly developed markers will be useful for further studies on the population structure and evolutionary history of the G. ussuriensis.

Biological Resources Potential and the Recent State of International Cooperation in Indonesia

  • Park, Chang-Ho;Junaedi, Ahmad;Lee, Mi-Ra;Lee, Yeon-Hee
    • Interdisciplinary Bio Central
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    • v.2 no.4
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    • pp.11.1-11.8
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    • 2010
  • Indonesia is a mega-biodiversity country having at least one trillion US Dollars value of biological resources per year over the next 20 years. Indonesia is home to 11 percent of the world's flowering plant species and 12 percent of all mammals. Many of Indonesia's species and more than half of the archipelago's endemic plant species are found nowhere else on the Earth. This information is just a small portion of all higher plants and animals being found in Indonesia. Former studies described that total number of species in Indonesia is estimated more than 1,000,000 and most of them remain unknown scientifically. Most of lower plants and animals have not been studied yet, so that greatest portion of Indonesia's biological resources has never been assessed with respect to its economic value or conservation status. Many developed countries are building cooperation with Indonesia on resources, mainly in the fields of grant aid, socio-economic services, R & D, researcher exchange, technology transfer, infrastructure, education/training, finance, etc. Indonesia will obtain greater benefits and management of its biological richness via increasing its international capacity to add value and information to its biological diversity. These goals can be achieved by close international collaboration on search of important biological resources and other bioactive products that have potential economic values. Development of biological resource-based technology stands as the industry of the $21^{st}$ century and, therefore, Indonesia has a unique opportunity to lead the process in the world.

Comprehensive and synthetic inventory of Dokdo Island, Republic of Korea

  • Ui Wook Hwang;Hyun Soo Rho;Bia Park;Eun Hwa Choi;Cho Rong Shin;Sa Heung Kim;Jongrak Lee;Hack Cheul Kim;Mann Kyoon Shin;Taeseo Park;Jumin Jun;Heegab Lee;Jong Eun Lee;Yoon Sik Oh;Jung-Goo Myoung;Chang Geun Choi;Jin Hee Park;Seon-joo Park;Jimin Lee;Jaeho Lee;Hyeok Yeong Kwon;Kyu Tae Park;Chun Woo Lim;Seung Wook Jung;Mi Jin Lee;Yucheol Lee;Yeongheon Shin;Hee-Jung Choi;Young Wook Lee;Hyun Jong Kil;Jin-Han Kim;Myung-Suk Kang;Eun-Young Lee;Sang-Hwa Lee;Young Hyo Kim;Jongwoo Jung;Kuem Hee Jang;Young Jin Lim;Shi Hyun Ryu;Won-Gi Min;Joo Myun Park;Hyojin Lee;Minsu Woo;Yun-Bae Kim;Sehun Myoung
    • Journal of Species Research
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    • v.12 no.spc
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    • pp.1-69
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    • 2023
  • This study aims to establish a comprehensive, synthetic inventory system for the fauna and flora of Dokdo Island, Republic of Korea, which has been conducted by a specialized research group consisting of more than 50 experts. The research was conducted over five years(2015-2019) and supported by the National Institute of Biological Resources, Ministry of Environment, Republic of Korea. All possible publications on the fauna and flora of Dokdo Island over the last 68 years from 1952 to 2020 were reviewed. As a result, 1,302 species were found on Dokdo Island during the study period. An updated list of 1,963 species was created. This is expected to be of great help for the conservation and national publicity of important indigenous biological resources of Dokdo Island.