• 한국축산식품학회지
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• 제37권1호
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• pp.62-70
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• 2017

The aim of this study was identifying a suitable food grade enzymes to hydrolyze whey protein concentrates (WPCs), to give the highest bioactivity. WPCs from ultrafiltration retentate were adjusted to 35% protein (WPC-35) and hydrolyzed by enzymes, alcalase, ${\alpha}-chymotrypsin$, pepsin, protease M, protease S, and trypsin at different hydrolysis times (0, 0.5, 1, 2, 3, 4, and 5 h). These 36 types of hydrolysates were analyzed for their prominent peptides ${\beta}-lactoglobulin$ (${\beta}-Lg$) and ${\alpha}-lactalbumin$ (${\alpha}-La$), to identify the proteolytic activity of each enzyme. Protease S showed the highest proteolytic activity and angiotensin converting enzyme inhibitory activity of IC50, 0.099 mg/mL (91.55%) while trypsin showed the weakest effect. Antihypertensive and antioxidative peptides associated with ${\beta}-Lg$ hydrolysates were identified in WPC-35 hydrolysates (WPH-35) that hydrolyzed by the enzymes, trypsin and protease S. WPH-35 treated with protease S in 0.5 h, responded positively to usage as a bioactive component in different applications of pharmaceutical or related industries.

• Journal of Ginseng Research
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• 제44권4호
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• pp.527-537
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• 2020

Panax ginseng, a medicinal plant, has been used as a blood-nourishing tonic for thousands of years in Asia, including Korea and China. P. ginseng exhibits adaptogen activity that maintains homeostasis by restoring general biological functions and non-specifically enhancing the body's resistance to external stress. Several P. ginseng effects have been reported. Korean Red Ginseng, in particular, has been reported in both basic and clinical studies to possess diverse effects such as enhanced immunity, fatigue relief, memory, blood circulation, and anti-oxidation. Moreover, it also protects against menopausal symptoms, cancer, cardiac diseases, and neurological disorders. The active components found in most Korean Red Ginseng varieties are known to include ginsenosides, polysaccharides, peptides, alkaloids, polyacetylene, and phenolic compounds. In this review, the identity and bioactivity of the non-saponin components of Korean Red Ginseng discovered to date are evaluated and the components are classified into polysaccharide and nitrogen compounds (protein, peptide, amino acid, nucleic acid, and alkaloid), as well as fat-soluble components such as polyacetylene, phenols, essential oils, and phytosterols. The distinct bioactivity of Korean Red Ginseng was found to originate from both saponin and non-saponin components rather than from only one or two specific components. Therefore, it is important to consider saponin and non-saponin elements together.

• Macromolecular Research
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• 제17권6호
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• pp.378-387
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• 2009

This research compared three methods for producing and processing nanocomposite polypropylene filament yarns with permanent antimicrobial efficiency. The three methods used to mix antimicrobial agents based on silver nano particles with PP were as follows: 1) mixing of PP powder and inorganic nanocomposite filler with the appropriate concentration using a twin-screw extruder and preparing granules, 2) method 1 with a singlerather than twin-screw extruder, and 3) producing the masterbatch by a twin-screw extruder and blending it with PP in the melt spinning process. All pure polypropylene samples and other combined samples had an acceptable spinnability at the spinning temperature of $240^{\circ}C$ and take-up speed of 2,000 m/min. After producing as-spun filament yarns by a pilot plant, melt spinning machine, the samples were drawn, textured and finally weft knitted. The physical and structural properties (e.g., linear density, tenacity, breaking elongation, initial modulus, rupture work, shrinkage and crystallinity) of the as-spun and drawn yarns with constant and variable draw ratios (the variable draw ratio was used to gain a constant breaking elongation of 50%) were investigated and compared, while DSC, SEM and FTIR techniques were used to characterize the samples. Finally, the antibacterial efficiency of the knitted samples was evaluated. The experimental results revealed that the crystallinity reduction of the as-spun yarn obtained from method 1 (5%) was more than that of method 2 (3%), while the crystallinity of the modified as-spun yarns obtained with method 3 remained unchanged compared to pure yarn. However, the drawing procedure compensated for this difference. By applying methods 2 and 3, the drawing generally improved the tenacity and modulus of the modified fibers, whereas method 1 degraded the constant draw ratio. Although the biostatic efficiency of the nanocomposite yarns was excellent with all three methods, the modified fabrics obtained from methods 1 and 2 showed a higher bioactivity.

• 한국결정성장학회지
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• 제22권2호
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• pp.99-104
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• 2012

BCP(biphasic calcium phosphate) 분말을 제조하기 위하여 $Ca(NO_3)_2{\cdot}4H_2O$, $(NH_4)_2HPO_4$를 출발 물질로 공침법(co-precipitation process)을 이용하여 합성하였다. 합성된 분말의 열처리 전 후의 HAp(Hydroxyapatite)/${\beta}$-TCP(${\beta}$-Tricalcium phosphate) 결정상 비율 및 분광학적 특성을 XRD 및 FT-IR를 이용하여 분석하였다. BCP 분말의 생체활성 거동을 평가하기 위하여 Hanks' Balanced Salt Solution(HBSS)에 침적시켜 시간에 따라 형상, 이온농도의 변화 및 결정상을 분석한 결과 낮은 결정화도의 HAp가 생성됨을 확인하였다. BCP분말의 세포독성 평가에서도 대조군에 비하여 세포성장률이 우수함을 관찰하였다.

• 한국세라믹학회지
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• 제48권3호
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• pp.217-221
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• 2011

Glass-ceramic materials, which consist of glass matrix phase containing crystalline ${\beta}-Ca_3(PO_4)_2$ and ${\beta}-Ca_2P_2O_7$, have been prepared by heating at $750-900^{\circ}C$ of calcium phosphate invert glasses in the silica-free $CaO-P_2O_5-TiO_2-Na_2O$, system. With increasing heating temperature from 750 to $900^{\circ}C$, the crystallite size of precipitated ${\beta}-Ca_3(PO_4)_2$ in glass with $55CaO{\cdot}35P_2O_5{\cdot}3TiO_2{\cdot}7Na_2O$ (mol%) composition increased from 48 to 91 nm. With the extension of the immersion time in dilute acetic acid solution (pH = 5) to ${\geq}$200 min, the degree of dissolution of $Ca^{2+}$ and $P^{5+}$ ions in the glass-ceramics was linearly increased and the solution was constantly maintained at pH = ~7. Biomimetic nanostructured (62-88 nm in average dia.), sphere-shaped hydroxyapatite was homogeneously formed on the surface of the glass-ceramics when socked for 7-14 days in a Hanks' solution, indicating bioactivity of the prepared glass-ceramics.

• 한국결정성장학회지
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• 제20권5호
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• pp.243-248
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• 2010

Si-BCP(si-substituted biphasic calcium phosphate)를 제조하기 위하여 $Ca(NO_3)_2{\cdot}4H_2O$, $(NH_4)_2HPO_4$, $Si(OC_2H_5)_4$을 출발 물질로 공침법(co-precipitation process)을 이용하여 합성하였다. 합성된 분말의 HAp/${\beta}$-TCP 결정상 비율 및 Si-P 치환 거동은 X-선 회절 상 분석을 이용하여 측정하였고, silicon ion 첨가 시 나타나는 BCP의 화학적 결합거동을 측정하기 위하여 FT-IR를 사용하여 분석하였다. 또한 Si-BCP 분말의 in-vitro 생분해거동 및 생체활성도를 측정하기 위하여 제조된 분말을 SBF(simulated body fluid) solution에 침적시킨 뒤 분말의 형상과 구성 성분은 SEM과 EDS를 통하여 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.600-608
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• 2013

Amide analogs of tridecapeptide ${\alpha}$-factor (WHWLQLKPGQPMYCONH$_2$) of Saccharomyces cerevisiae, in which Trp at position 1 and 3 were replaced with other residues, were synthesized to ascertain whether cooperative interactions between two Trp residues occurred upon binding with its receptor. Analogs containing Ala or Aib at position 3 of the peptide $[Ala_3]{\alpha}$-factor amide (2) and $[Aib_3]{\alpha}$-factor amide (5) exhibited greater decreases in bioactivity than analogs with same residue at position one $[Ala^1]{\alpha}$-factor amide (1) and $[Aib^1]{\alpha}$-factor amide (4), reflecting that $Trp^3$ may plays more important role than $Trp^1$ for agonist activity. Analogs containing Ala or Aib in both position one and three 3, 6 exhibited complete loss of bioactivity, emphasizing both the essential role and the combined role of two indole rings for triggering cell signaling. In contrast, double substituted analog with D-Trp in both positions 9 exhibited greater activity than single substituted analog with D-Trp 8 or deleted analog 7, reflecting the combined contribution of two tryptophane residues of ${\alpha}$-factor ligand to activation of Ste2p through interaction with residue $Tyr^{266}$ and importance of the proper parallel orientation of two indole rings for efficient triggering of signal G protein coupled activation. Among ten amide analogs, $[Ala^{1,3}]{\alpha}$-factor amide (3), $[Aib^{1,3}]{\alpha}$-factor amide (6), [D-$Trp^3]{\alpha}$-factor amide (8) and [des-$Trp^1,Phe^3]{\alpha}$-factor amide (10) were found to have antagonistic activity. Analogs 3 and 6 showed greater antagonistic activity than analogs 8 and 10.

• 농업생명과학연구
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• 제46권6호
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• pp.157-163
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• 2012

새송이버섯(Pleurotus eryngii)은 인기 있는 식용버섯 중의 하나로서 항산화, 항암 또는 면역조절 기능 등의 인체에 유익한 생리활성 기능을 지닌다. 본 연구에서는 새송이버섯 균사체 배양 시 커피음료 생산과정으로부터 폐기되는 커피박(spent coffee ground: SCG) 첨가에 의한 균사체의 생장과 기능성의 변화를 조사하였다. 그 결과, SCG(1-10%, w/v) 첨가 시 생장속도가 상당히 증가하는 경향을 나타내었다. 특히 1% SCG 첨가 시 무첨가에 비해 건조중량이 2.5배 증가함으로써 SCG가 탁월한 새송이버섯 균사체의 생장촉진제 기능이 제시되었다. SCG 첨가에 의하여 균사체의 polysaccharide 함량은 변화가 없었으나 polyphenol량 및 항산화능의 증대를 확인할 수 있었다.

• 한국약용작물학회지
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• 제27권3호
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• pp.194-201
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• 2019

Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity ($IC_{50}$; $15.74{\mu}g/m{\ell}$), and it also had the highest total phenolic and flavonoid content ($220.95mg{\cdot}GAE/g$, and $36.46mg{\cdot}QE/g$ extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 mg/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.

• 대한치과보철학회지
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• 제57권1호
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• pp.88-94
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• 2019

심미적 치료에 대한 요구가 늘어나면서 높은 강도와 심미성을 갖는 지르코니아의 요구도 증가하고 있다. 이러한 흐름에 비추어 지르코니아의 생체적 합성을 평가하는 것은 중요한 일이다. 이번 논문에서는 지르코니아의 생체적합성에 대한 in vivo 실험에 대한 문헌 연구를 진행하였다. In vivo 실험에서 연조직, 경조직에 대한 지르코니아의 생체적합성을 확인할 수 있었다. 다양한 실험동물 및 환자에서 진행된 연구의 대다수에서 지르코니아의 높은 생체적합성이 보고되었으며, 신생골 합성 및 골부착의 면에서 티타늄과 유사한 성질을 보였다. 한편, 지르코니아는 임플란트로도 활용할 수 있다. 임플란트로 활용하기 위해 HA (hydroxyapatite)를 처리하여 생체활성을 높이는 다양한 방식이 제안되고 있다. 하지만 기존의 티타늄 임플란트에 HA를 코팅하는 방식은 낮은 결합강도 및 HA의 변성으로 인한 문제점이 있었기 때문에 HA-지르코니아 composite, HA-coated 지르코니아, HA-지르코니아 functionally graded material (FGM) 또는 알루미나 개재 HA-지르코니아 등의 새로운 방식이 연구되고 있다. 이러한 방식들은 보다 높은 결합강도를 지니고 있으며, 높은 생체적합성을 보여주고 있다.