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http://dx.doi.org/10.7783/KJMCS.2019.27.3.194

Comparison of Anti-oxidant and Anti-inflammatory Activities of Methanolic Extracts Obtained from Different Parts of Cotoneaster wilsonii Nakai  

Yoo, Nam Ho (Division of Bio-Resource Sciences, Kangwon National University)
Kim, Hee Kyu (Gangwondo Forest Science Institute)
Lee, Chan Ok (Gangwondo Forest Science Institute)
Park, Ju Hee (Lambio Institute, Kangwon National University)
Kim, Myong Jo (Division of Bio-Resource Sciences, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.27, no.3, 2019 , pp. 194-201 More about this Journal
Abstract
Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity ($IC_{50}$; $15.74{\mu}g/m{\ell}$), and it also had the highest total phenolic and flavonoid content ($220.95mg{\cdot}GAE/g$, and $36.46mg{\cdot}QE/g$ extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 mg/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.
Keywords
Cotoneaster wilsonii Nakai; Anti-oxidant; Anti-inflammatory; Caffeic Acid; Chlorogenic acid; Ferulic Acid; Gallic Acid; High Performance Liquid Chromatography; Syringic Acid;
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