• Title/Summary/Keyword: BioSIM

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대기압 플라즈마로 폐 암세포(H460)와 폐 정상세포(L132) 처리시, OH radical density에 따른 Cell 변화 측정

  • Park, Dae-Hun;Kim, Yong-Hui;Sim, Geon-Bo;Baek, Gu-Yeon;Eom, Hwan-Seop;Choe, Eun-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.184.2-184.2
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    • 2013
  • 대기압 플라즈마와 생체용액과의 상호작용은 Bio-medical 분야에서 주목 받고 있다. 대기압 플라즈마는 전자온도가 고온 플라즈마 보다 상대적으로 낮기 때문에 생체에 적용하기가 적합하다. 따라서 플라즈마가 세포에 미치는 영향을 관측하기 위해서 대기압 플라즈마를 이용하여 생체용액과의 반응을 살펴보고자 한다. Ar gas를 이용하여 플라즈마를 발생시켜 생체용액 표면을 처리하고 OES (Optical Emission Spectroscopy)을 이용해 방출 선을 조사했다. Ar 기체를 이용한 대기압 플라즈마를 사용하여 다른종류의 용액내의 OH Radical Density를 측정하였다. 용액으로는 DI (deionized) water 와 PBS (1x phosphate buffered saline)를 사용하였다. Ar gas를 200 sccm (cm3/min) 으로 흐르게 하였을 때, DI water의 OH Radical Density 는 4.33×1016cm3 으로 측정되었으며, 자외선 흡수분광법으로 측정한 완충용액인 PBS의 OH Radical Density 측정값은 $1.87{\times}10^{16}cm^{-3}$ 이다. 이런 특성을 기반으로, PBS 용액내의 H460 (Lung Cancer Cell) 와 L132 (Lung Normal Cell)을 깊이와 시간에 따라 대기압 플라즈마로 처리하여 cell의 변화를 보았다. 실험 각각의 조건은 깊이를 2 mm, 4 mm, 6 mm이며 시간은 10 sec, 30 sec, 60 sec 로 설정하였다. 표면으로부터의 깊이가 2 mm, 4 mm, 6 mm 일때 의 OH Radical Density는 각각 1.87×1016cm3, 0.5×1016cm3, 0으로써 용액이 깊어질수록 OH Radical Density가 감소함을 볼 수 있다. OH radical density가 높은 2 mm 에서, 처리한 시간이 길어질수록 Cell 은 영향을 많이 받음을 관찰 할 수 있었다. H460 이 L132 보다 플라즈마에 영향을 많이 받음을 확인하였다. 특성변화를 알아보기 위하여 raman spectroscopy, flow cytometry, electron spin resonance로 측정한다.

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Developing a New BNR (Parallel BNR) Process by Computer Simulation (컴퓨터 시뮬레이션을 이용한 신 생물학적 고도처리 (병렬 고도처리) 공법 개발)

  • Lee, Byonghi;Lee, Yong-Woon
    • Journal of Korean Society of Water and Wastewater
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    • v.16 no.6
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    • pp.670-678
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    • 2002
  • Since Korean government imposed a stricter regulation on effluent T-N and T-P concentrations from wastewater treatment plant, a new process has to be developed to meet these rules and this process should remove T-N and T-P, economically, from weak wastewater that is typical for Korea's combined sewer system sewage. In this study, a computer simulator, BioWin from EnviroSim, Inc. was used. Three processes - A2/O, Modified Johannesburg, UCT- had been simulated under same operational conditions and a new process - Parallel BNR Process - had been developed based on these simulation results. The Parallel BNR process consists of two rows of reactors: One row has anaerobic and aerobic reactors in series, and the other row has RAS anoxic1 and RAS anoxic2 reactors in series. In order to ensure anaerobic state in anaerobic tank, a part of influent is fed to RAS anoxic1 tank in second row. This process had been simulated under same conditions of other three processes and the simulation results were compared. The results showed that three existing processes could not perform biological phosphorus removal when the average influent was fed at any operation temperatures. However, the Parallel BNR process was found that biological phosphorus removal could be performed when both design and average influent were fed at any operation temperatures. This process showed the T-N concentration in effluent had a maximum value of 15mg/L when design influent was fed at 13C and a minimum value of 14mg/L when average influent was fed at 20C. Also, T-P concentrations had a maximum value of 1.3mg/L when average influent was fed at 20C and a minimum value of 1.1mg/L when design influent was fed at 13C. Based on these results, we found that this process can remove nitrogen and phosphorus biologically under any operational conditions.

Development of Pretreatment and Mixed Culture Processes for Plant Originated Lactic Acid to Produce a Functional Lactic acid Beverage (대두 전처리 공정 및 식물 유래 유산균 혼합 배양에 의한 기능성 유산균 음료 생산기술 개발)

  • Cho, Eun-Kyung;Cho, Hyung-Yong;Kim, Byeong-Cheol;Shin, Hae-Hun;Cho, Seok-Cheol;Kook, Moo-Chang;Pyun, Yu-Ryang
    • The Korean Journal of Food And Nutrition
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    • v.24 no.1
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    • pp.117-123
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    • 2011
  • This study was conducted to establish process conditions for plant-originated lactic acid production using a mixed culture of plant originated lactic acid bacteria, Lactobacillus sakei B2-16, and Lactobacillus plantarum P23, which were isolated from kimchi, and Bacillus subtilis, which was TP6 isolated from Denjang. Soybean medium was pretreated for 10 minutes at 110C and hydrolyzed with 0.2%(w/v) cellulase at 5560C for at least 2 hrs. The quality of the final fermentation product was influenced by the inoculation ratio of the Lactobacillus sakei B2-16, Lactobacillus plantarum P23, and Bacillus subtilis TP6. The optimum microorganism inoculation ratio was 1:0.7:0.3, Lactobacillus sakei B2-16: Lactobacillus plantarum P23: Bacillus subtilis TP6, respectively. The sensory characteristics of the product were a refreshing sourness and a soft flavor.

Effect of Slander Glasswort Extract Yogurt on Quality during Storage (함초 추출물 첨가가 요구르트 저장 중의 품질 특성에 미치는 영향)

  • Cho, Young-Sim;Kim, Soon-Im;Han, Young-Sil
    • Korean journal of food and cookery science
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    • v.24 no.2
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    • pp.212-221
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    • 2008
  • This study was performed in an effort to create a functional and stable yogurt product containing slander glasswort extract. The extract was added to milk at concentrations of 0.25, 0.5, and 1.0% (w/v), which was then fermented with lactic acid bacteria (Lactobacillus bulgaricus and Streptococcus thermophilus) at 42C for 6 hr. During a period of 15 days of storage, the quality characteristics of the yogurt samples were evaluated in terms of acid production (pH and titratable acidity), levels of lactic acid bacteria, color values, viscosity, and sensory characteristics. There were no significant differences in pH during the storage period; however, the control yogurt presented the highest pH value. The 1.0% slander glasswort yogurt had the highest titratable acidity; but again, there were no significant differences among the yogurts. The 1.0% slander glasswort yogurt also had the highest level of lactic acid bacteria, and both the control and slander glasswort-containing yogurts had increasing levels of lactic acid bacteria over the storage period. The 1.0% slander glasswort yogurt had the lowest L-value and highest a- and b-values. And the slander glasswort yogurts presented lower viscosity values than the control. In sensory evaluations, the 0.25% slander glasswort yogurt scored higher than the other groups for color, flavor, viscosity, sweetness, sourness, and overall palatability. The final sensory results indicated that the 0.25% slander glasswort yogurt was superior.

Effect of Decomposition on Nitrogen Dynamics in Soil Applied with Compost and Rye

  • Ko, Byong-Gu;Kim, Myung-Sook;Park, Seong-Jin;Yun, Sun-Gang;Oh, Taek-Keun;Lee, Chang Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.6
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    • pp.648-657
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    • 2015
  • Soil organic matter (SOM) plays an important role in the continuous production and environmental conservation in arable soils. In particular, the decomposition of organic matter in soil might promote soil organic matter and fertility due to the mineralization of N. In this study, to evaluate the effect of organic matter amendment on the C mineralization and N dynamic, CO2C flux, extractable N and N2O emission were determined using closed chamber for 4 weeks at 10, 15, 20C of incubation temperature after the mixture of 2Mgha1 rice straw compost and rye in sandy loam and clay loam. Regardless of soil texture, decomposition rates of rice straw compost and rye at 1020C of incubation temperature ranged from 0.9 to 3.8% and 8.8 to 20.3%, respectively. Rye application in soil increased NH4N and NO3N content as well as the N2O emission compared to the rice straw compost. After incubation for 4 weeks, total C content in two soils was higher in rice straw compost than in rye application. In conclusion, application of rice straw compost and rye to soil was able to improve the soil organic matter and fertility. However, organic matter including the recalcitrant compounds like rice straw compost would be effective on the management of soil organic matter and the reduction of greenhouse gases in soil.

Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

  • Kim, Hak-Gon;Song, Hyun-Jin;Jeong, Mi-Jin;Sim, Seon-Jeong;Park, Dong-Jin;Yang, Jae-Kyung;Yoo, Seok-Bong;Yeo, Jin-Ki;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of Forest and Environmental Science
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    • v.27 no.3
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    • pp.143-149
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    • 2011
  • The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

Analysis of a 6th Industrialization Model in the Saemangeum Grain Complex (새만금 복합곡물단지의 6차산업화 모델 분석)

  • Kim, Yooan;Jung, Chanhoon;Kim, Solhee;Kim, Chanwoo;Suh, Kyo
    • Journal of Korean Society of Rural Planning
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    • v.25 no.2
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    • pp.63-74
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    • 2019
  • As the awareness of food security has increased, the Korean government has established national projects, such as Saemangeum proclaimed land, to secure sources of grain. Saemangeum is a large-scale agricultural area that was constructed to maintain preparedness for unstable food markets. This study aims to develop a 6th Industrialization Model (SIM) for Saemangeum Grain Complex by applying feasible strategies to wheat and two-rowed barley which have low self-sufficiency rates. In addition, this study estimates the potential economic value of each development strategy associated with a sixth industrialization model to create higher added values from production, processing and tourism experiences. The strategic plan for primary, secondary, and tertiary industries is to combine cultivating and processing wheat and two-rowed barley for sales and linking them to tourism experience. This study shows value added from the combination of the primary, secondary and tertiary industry of wheat and two-rowed barley are 7.5 and 23.0 times more than those of the primary and tertiary industry combination, respectively. Through branding Saemangeum Grain Complex's products, such as Saemanguem bread and craft beer, would further enhance the economic benefits derived from the complex.

The fabrication of bulk magnet stacked with HTS tapes for the magnetic levitation

  • Park, Insung;Kim, Gwantae;Kim, Kyeongdeok;Sim, Kideok;Ha, Hongsoo
    • Progress in Superconductivity and Cryogenics
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    • v.24 no.3
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    • pp.47-51
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    • 2022
  • With the innovative development of bio, pharmaceutical, and semiconductor technologies, it is essential to demand a next-generation transfer system that minimizes dust and vibrations generated during the manufacturing process. In order to develop dust-free and non-contact transfer systems, the high temperature superconductor (HTS) bulks have been applied as a magnet for levitation. However, sintered HTS bulk magnets are limited in their applications due to their relatively low critical current density (Jc) of several kA/cm2 and low mechanical properties as a ceramic material. In addition, during cooling to cryogenic temperatures repeatedly, cracks and damage may occur by thermal shock. On the other hand, the bulk magnets made by stacked HTS tapes have various advantages, such as relatively high mechanical properties by alternate stacking of the metal and ceramic layer, high magnetic levitation performance by using coated conductors with high Jc of several MA/cm2, consistent superconducting properties, miniaturization, light-weight, etc. In this study, we tried to fabricate HTS tapes stacked bulk magnets with 60 mm × 60 mm area and various numbers of HTS tape stacked layers for magnetic levitation. In order to examine the levitation forces of bulk magnets stacked with HTS tapes from 1 to 16 layers, specialized force measurement apparatus was made and adapted to measure the levitation force. By increasing the number of HTS tapes stacked layers, the levitation force of bulk magnet become larger. 16 HTS tapes stacked bulk magnets show promising levitation force of about 23.5 N, 6.538 kPa at 10 mm of levitated distance from NdFeB permanent magnet.

Verification of Control Algorithm for Removing Oil Contaminant Factor from Proportional Pressure Control Valve (전자식 비례 압력제어밸브 내 오일 오염 입자 제거 제어 알고리즘 검증)

  • Cheon, Su Hwan;Park, Jin Kam;Jang, Kyoung Je;Sim, Sung Bo;Jang, Min Ho;Lee, Jin Woong
    • Journal of Drive and Control
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    • v.18 no.4
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    • pp.1-8
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    • 2021
  • An electro proportional pressure control valve is mainly used to control the clutch of an agricultural tractor's automatic transmission. During transmission, the operating, hydraulic oil is mix with many kinds of contaminants. The contaminants can be trapped between the valve body and spool of the proportional pressure control valve leading to abnormal operating conditions and finally critical damage to the transmission hydraulic system. The present study aimed to verify the valve control algorithm as a basic study of developing control logic that removes contaminants between the spool and the body of the proportional pressure control valve. To develop the algorithm, MATLAB/SIMULINK was used. PWM method was used to control the applied solenoid coil current. The effectiveness of the algorithm was verified by comparing the actual pressure of the normal valve with the actual pressure of the abnormal valve. Based on the present study findings, when the algorithm was applied, the response of the valve pressure according to the current became stable and oil contaminated particles were removed. In the future study, the control algorithm will be optimized for the stability of the proportional pressure reducing valve, and it will be verified in consideration with the driving of the clutch.

Residual Toxicity of Bifenthrin and Imidacloprid to Honeybee by Foliage Treatment (Bifenthrin과 Imidacloprid의 작물잎에서의 잔류량과 꿀벌에 대한 독성)

  • Cho, Kyung-Won;Park, Hyun-Ju;Bae, Chul-Han;Kim, Yeon-Sik;Shin, Dong-Chan;Lee, Seung-Yeol;Lee, Suk-Hee;Jung, Chang-Kook;Park, Yeon-Ki;Kim, Byung-Seok;Lee, Kyu-Seung
    • The Korean Journal of Pesticide Science
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    • v.14 no.3
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    • pp.226-234
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    • 2010
  • Foliage residue toxicity experiment was performed against honeybee (Apis mellifera) with bifenthrin, a synthetic pyrethroid insecticide with strong acute contact toxicity and imidacloprid, a neo-nicotinoid insecticide with strong acute oral toxicity to know the honeybee toxicity at the residue level on the leaves of alfalfa and apple. Also, the formulation differences to honeybee toxicity were investigated with WP (2%) and EC (1%) of bifenthrin and WP (10%) and SL (4%) of imidacloprid. Generally, foliage residual toxicity of honeybee and residual amounts of tested insecticides was higher in alfalfa leaves with large leaf area per unit weight than in apple leaves. While on the other hand, the only bifenthrin WP treatment showed higher honeybee toxicity on apple leaves than alfalfa. Although imidacloprid showed higher residue amounts ranged 4.925.4mgkg1 than bifenthrin ranged 0.612.7mgkg1 on the leaves, the residual toxicity to honeybee was lower than bifenthrin because of its strong penetration character. In conclusion, the residual toxicity of insecticide to honeybee could be affected by the contact and vaporized toxicity of chemical, the residual amounts on the surface of leaves, and the leaf area per unit weight and formulation differences.