• Title/Summary/Keyword: BioSIM

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Effect of Greenhouse Cooling and Transplant Quality Using Geothermal Heat Pump System (지열-열펌프 시스템의 온실냉방 및 육묘 효과)

  • Lee, Jae-Han;Lee, Yong-Beom;Kwon, Joon-Kook;Kang, Nam-Jun;Kim, Hak-Joo;Choi, Young-Hah;Park, Jin-Myeon;Rhee, Han-Cheol
    • Journal of Bio-Environment Control
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    • v.15 no.3
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    • pp.211-216
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    • 2006
  • This study was carried out to investigate the effect of greenhouse cooling by a geothermal heat pump system on greenhouse temperature and growth of vegetable transplants in summer season. Greenhouse air temperature in day time was 34C34C lower in fog plus shading system than in shading, while in night time that was 57C57C lower in geothermal heat pump (GHP) plus shading system compared to shading or fogplus shading. system. Plant height of cucumber, tomato and hot pepper transplants was shortened in GHP plus shading compared to shading or fog plus shading system. And Leaf area and dry weight were slightly decreased in GHP plus shading compared to the other systems. Therefore, healthy transplant index on cucumber, tomato and hot pepper was higher in GHP plusshading than in shading or fogplusshading system.

Isolation and Characterization of A Thiodiglycol-Degrading Cupravidus sp. (Thiodiglycol를 분해하는 Cupriavidus sp.의 분리와 특성)

  • Park, Jong-Deok;Kim, Jee-Cheon;Yoon, Ki-Hong
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.311-316
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    • 2007
  • A Gram-negative bacterium capable of degrading thiodiglycol (TDG), main hydrolysis product of sulfur mustard, was isolated from ginseng field in enrichment medium supplemented with TDG as carbon source. The isolate, WS-32, grew optimally at 3037C3037C and pH 6.0-8.0. It was found to be similar to the genus Cupriavi연 on the basis of 165 rRNA sequence, while its biochemical properties were highly homologous to Alcaligenes faecalis. The cell growth of WS-32 strain was slightly inhibited on LB broth by TDG, but the maximum level of its growth was maintained stably in the presence of TDG. After incubation of inoculated LB medium or uninoculated LB medium containing TDG for 2 days, TDG amount of the culture filtrate was analyzed to decrease noticeably by HPLC. TDG and alcohols were also oxidized by cell-free extract of the isolate with maximum activities at pH 8.0 and 45C45C.

연구논문 - "고온성 종균제를 이용한 제지폐수처리에 대한 연구(1)"

  • Lee, Seong-Ho
    • Environmental engineer
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    • s.323
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    • pp.22-27
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    • 2013
  • 종이 펄프 공장의 폐수는 다른 공업에 비해서 폐수량이 많고 오염도가 높다. 목재로부터 종이를 제조하는 경우에 수율은 종이의 종류와 제법에 의해 큰 차이가 있어서 한마디로 말하기는 어렵지만 평균 50~70%로 보는 경우, 목재 성분의 30~50%는 용해 또는 SS의 상태로 폐수중에 존재하기 때문에, 이들이 직접 하천이나 바다에 방류되면 오염의 원인이 된다. 그 때문에 각 공장에서는 목재자원의 종합적인 이용의 입장으로서 제품의 수율을 좋게 하고 동시에 폐수의 양을 감소시키고 폐수에서 유용한 자원을 회수하거나 증해약품의 회수 등을 위하여 연구 및 노력을 계속하고 있는 것이 현 상태이다. 우리 나라의 거의 모든 제지회사에서는 물리적, 화학적 처리법과 생물학적 처리법 중 호기성 처리방법이 주 처리방법으로 사용되고 있으며 탈묵 폐수나 리그닌을 다량 함유하는 폐수의 경우에는 혐기성 처리방법을 이용하기도 한다. 본 실험에서는 우유팩을 재생하여 화장지 제조의 원료로 이용하는 G제지공장의 폐수를 이용하였고, 공장의 원폐수를 부상법으로 1차 처리한 처리수를 실험실로 운반하여 활성슬러지법으로 실험하였다. 처리효율의 영향인자인 HRT를 G제지공장에 맞추어 12시간으로 조정하고 적절한 F/M비(0.23)를 위해 COD와 MLSS가 유지된 후부터 실험을 시작했다. 20C560C20C560C까지 10C10C간격으로 온도를 변화시키면서 미생물의 상태변화를 관찰하고, 또한 각 용도에서의 처리수질을 비교해 보았다. 실험조건은 pH는 중성상태(6.5~7.5), DO는 24mg/24mg/, 영양원은 BOD : N : P = 100 : 5 : 1로 맞추고 BOD, COD, SS, SVI를 측정하였으며, 현미경을 통하여 미생물과 Floc를 관찰하였다. 20C20C부터 종균제를 초기 운전시 200mg/200mg/ (분말상)을 폭기조에 투여하고 매일 200mg/200mg/를 정기적으로 투입하여 온도변화에 따른 미생물의 상태 및 처리수질을 비교해보았다. 특히 종균제로는 기존의 G제G지공장에서 사용하는 신일 미생물(Bio sound S)종균제와 청림C&C의 종균제(Cell bio P)를 비교 관찰하였다. 고온성 종균제를 두배 투입하여 투입량에 대한 처리효율을 비교하였으며 종균제를 사용하지 않고 운용함으로써 그 차이도 비교할 수 있었다.

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Discharge Variation of Perforated Hoses and Drip Irrigation Systems for Protected Cultivation (시설재배용 분수호스 및 점적관수 시스템의 관수균일도 분석)

  • Nam, Sang-Woon;Kim, Young-Shik
    • Journal of Bio-Environment Control
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    • v.16 no.4
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    • pp.297-302
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    • 2007
  • Discharge variations of perforated hose and drip irrigation systems were examined to evaluate irrigation uniformity at different pressures and length of branch line. Evaluation using statistical uniformity indicated that button drippers performed at excellent level but drip tapes and drip hoses were a little lower level. Nominal discharge of drip irrigation systems showed at the high side within the range of regulating pressure provided by the manufacturer. It is desirable that the length of branch line for drip hose, drip tape, and button dripper should be limited to 50 m, 70 m, and 100 m, respectively. Irrigation uniformity of perforated hoses showed very low level. So it is recommended that the length of branch line for perforated hoses should be limited to 3035m3035m.

Growth Chracteristics, Vitamin C Content and Antioxidative Activity among Local Strains of Sedum sarmentosum (돌나물 수집종간 생육특성, 비타민 C 함량 및 항산화 활성)

  • Kim, Hyo-Jin;Jung, Seung-Ho;Bae, Jong-Hyang;Lee, Seung-Yeob
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.110-115
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    • 2008
  • To evaluate growth chracteristics, vitamin C content and antioxidative activity of Sedum sarmentosum, 13 local strains were cultivated in the field. Keumsan, Bucheon, and Pocheon strains had narrow and oblong leaf, long hight, and slender stem compared to the other strains. Wanju and Gunsan strains were not formed flower stalks, and did not flower during the flowering time. Vitamin C contents were ranged from 407.8 to 434.4mgkg1434.4mgkg1, and it was high in Gwangyang, Buan, Wanju, and Gunsan strains. But vita min C contents didn't show a significant difference among strains. In antioxidative activity between fresh and freeze dried shoots, electron donating abilities (EDA) were ranged from 45.0 to 66.5% in MeOH extract of fresh shoot, and from 61.3 to 66.5% in MeOH extract of freeze dried shoot. Gwangyang, Suncheon, Wando strains showed high EDA compared to the others. Vitamine C content and antioxidant activity were highest in Gwangyang strain.

Head Mouse System Based on A Gyro and Opto Sensors (각속도 및 광센서를 이용한 헤드 마우스)

  • Park, Min-Je;Yoo, Jae-Ha;Kim, Soo-Chan
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.46 no.4
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    • pp.70-76
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    • 2009
  • We proposed the device to control a computer mouse with only head movements and eye blinks so that disabilities by car or other accidents can use a computer. The mouse position were estimated from a gyro-sensor which can measure head movements, and the mouse events such as click/double click were from opto sensors which can detect the eyes flicker, respectively. The sensor was mounted on the goggle in order not to disturb the visual field. There was no difference in movement speed between ours and a general mouse, but it required 34 more times in the result of the experiment to evaluate spatial movements and events detection of the proposed mouse because of the low accuracy. We could eliminate cumbersome work to periodically remove the accumulated error and intuitively control the mouse using non-linear relative point method with dead zones. Optical sensors are used in the event detection circuitry designed to remove the influence of the ambient light changes, therefore it was not affected in the change of external light source.

Effect of Silver Ion Solution on the Inhibition of Microcystis Growth (은이온 수용액의 Microcystis 생장 억제 효과)

  • Choi, Gang-Guk;Lee, Sang-Hun;Bae, Kie-Seo;Shin, Jae-Ki;Oh, Hee-Mock
    • Korean Journal of Environmental Biology
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    • v.26 no.3
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    • pp.183-191
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    • 2008
  • The effect of silver ion solution on the growth of Microcystis aeruginosa UTEX 2388 (cyanobacterium) and Chlorella sp. KCTC AG20136 (green alga) was investigated using separated and mixed culture in filtered natural water and BG11 medium. In separated culture, M. aeruginosa UTEX 2388 and Chlorella sp. KCTC AG20136 were found to be sensitive to 0.01 and 0.1 mg L11 of silver ion, respectively. Also, the silver ion concentrations for the growth inhibition of M. aeruginosa UTEX 2388 and Chlorella sp. KCTC AG20136 in the mixed culture were same in separated culture. Cyanobacteria were more sensitive to the silver ion solution than green algae. In bloom sample, the minimal inhibition concentration of silver ion solution for the low Chl-αα sample (110190 μgμg L11) and high Chl-αα sample (1,5001,900 μgμg L11) was about 0.1 and 3.0 mg L11, respectively. The silver ion concentration for the inhibition of algal bloom sample was affected by the algal biomass. In order to use silver ion solution for the control of algal bloom, the silver ion concentration must be determined in consideration of a minimal effect on the environment.

A Frequency-dependent Single Cell Impedance Analysis Chip for Applications to Cancer Cell and Normal Cell Discrimination (주파수에 따른 단일세포의 임피던스 분석칩 및 암세포와 정상세포의 구별에의 적용)

  • Chang, YoonHee;Kim, Min-Ji;Cho, Young-Ho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.63 no.12
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    • pp.1671-1674
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    • 2014
  • This paper presents a frequency-dependent cell impedance analysis chip for use in cancer and normal cell discrimination. The previous cell impedance analysis chips for flowing cells cannot allow enough time for cell-to-electrode contact to monitor frequency-dependent impedance response. Another type of the previous cell impedance analysis chips for the cells clamped by membranes need complex sample control for making stable cell-to-electrode contact. We present a new impedance analysis chip using the microchamber array, on which a PDMS cover is placed to make stable cell-to-electrode contact for the individual cell trapped in each microchamber; thus achieving frequency-dependent single-cell impedance analysis without complex sample control. Compared to the normal cells, the magnitude of NHBE cells is 60.0797.41kΩ60.0797.41kΩ higher than A549 cells in the frequency range of 95.6 kHz~2MHz and the phase of NHBE is 3.9620.83.9620.8 higher than A549 cells in the frequency range of 4.37 kHz~2MHz, respectively. It is demonstrated experimentally that the impedance analysis chip performs frequency-dependent cell impedance analysis by making stable cell-to-electrode contact with simple sample control; thereby applicable to the normal cell and cancer cell discrimination.

Anti-cancer Activity of Korean Local Plant Extracts Inducing Apoptosis in Various Carcinoma Cells (암세포 특이적 세포 사멸을 유도하는 자생식물 추출물의 항암 효과)

  • Yoon, Yi-Kwan;Lee, Seung-Eun;Lee, Dong-Jin;Rho, Mun-Chual;Sung, Jung-Suk;Park, Chung-Berm;Jang, Young-Joo
    • Korean Journal of Pharmacognosy
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    • v.40 no.1
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    • pp.6-12
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    • 2009
  • Thirty five methanol extracts from 19 natural local plants, which have been used as traditional anti-cancer medicine, were prepared. They were analyzed the cytotoxic effects on primary fibroblast cells and carcinoma cells. The root extract of Solanum nigrum were highly toxic in both cell lines with IC50IC50 values of less than 0.01μg/μl0.01μg/μl, and 26 of 35 extracts were toxic in all cells with IC50IC50 values of 0.12μg/μl0.12μg/μl. Three extracts including the fruit extracts of Solanum nigrum and Morus alba had no cytotoxic activity in both cell lines. Five of 35 extracts were highly toxic in cancer cells than in primary cells. Because primary cells were more resistant on these extracts, the five extracts were selected for anti-cancer agent candidates. Apoptosis or programmed cell death has an essential role in chemotherapy-induced tumor cell killing. Recently, inducers of apoptosis have been used in cancer therapy. When two of 5 cancer cell-specific cytotoxic extracts (Ulmus parvifolia and Zelkova serrata) were treated in concentration of 0.020.1μg/μl0.020.1μg/μl, apoptosis were increased at 3-5 times in cancer cell lines. Finally, the apoptotic effects of these extracts were confirmed by cleavages of both poly-(ADP-ribose)-polymerase and caspase-3 as apoptotic markers. In this report, we suggested that two of 35 medicinal herb extracts can be useful anti-cancer drug candidates inducing apoptosis in several carcinoma cell lines.

Effect on Quality of Pan-Fired Green Tea at Different Pan-Firing Conditions (덖음차 제조공정 중 덖음 횟수가 녹차의 품질에 미치는 영향)

  • Park Jang-Hyun;Kim Yong-Ok;Jung Jong-Mo;Seo Jong-Bun
    • Journal of Bio-Environment Control
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    • v.15 no.1
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    • pp.107-113
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    • 2006
  • These studies were carried out to investigate the quality of green tea processed by different pan-firing hand rolling and to select the best conditions during the procedure of green tea processing the results were as follows. At harvestion tea plant leaf, tea plants had 4 leaves. each leaf size was 7.5 cm'2.6 cm, and weight of 100 buds 46.6 g. The increase of pan-fired time decreased in the moisture content and yield of green tea. After processing green tea, 5 times pan-fired one had much more contents of total nitrogen (T-N:5.31%), caffeine (2.00%) and total amino acid (T.A.A. : 3,046 mg%) and less content of tannin (13.41%) than the other treatments. but 3 times pan-fired one had the highest contents of vitamin C (204.3 mg%) and chlorophyll (365.0 mg%) among three kinds pan-firing method. Sixteen kinds of free amino acid including theanine as a good taste component of pan-fired green tea were isolated and measured by HPLC. Free amino acid (FAA) contents were in the range of 2,6832,925 mg% and among various pan-firing methods, 5 tims pan-fired one had the highest content of F.A.A. Among these free amino acids, theanine occupied as much as 56.461.3 of F.A.A. Lightness, yellowness and redness of 5 times pan-fired green tea were middle value among three different treatments. Besides, it gained the highest score of sensory evaluation among them. These above results suggested that 5 times pan-fired green tea was considered to be the best level as pan-firing times of green tea processing.