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Anti-cancer Activity of Korean Local Plant Extracts Inducing Apoptosis in Various Carcinoma Cells  

Yoon, Yi-Kwan (The College of Bio-Resources Science, Dankook University)
Lee, Seung-Eun (Department of Herbal Crop Research, RDA)
Lee, Dong-Jin (The College of Bio-Resources Science, Dankook University)
Rho, Mun-Chual (Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology)
Sung, Jung-Suk (Department of National Agrobiodiversity Center, RDA)
Park, Chung-Berm (Department of Herbal Crop Research, RDA)
Jang, Young-Joo (Cell cycle & Signal Transduction Laboratory, Department of Nanobiomedical Science & Institute of Tissue Regeneration Engineering, Dankook University)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.1, 2009 , pp. 6-12 More about this Journal
Abstract
Thirty five methanol extracts from 19 natural local plants, which have been used as traditional anti-cancer medicine, were prepared. They were analyzed the cytotoxic effects on primary fibroblast cells and carcinoma cells. The root extract of Solanum nigrum were highly toxic in both cell lines with $IC_{50}$ values of less than $0.01{\mu}g/{\mu}l$, and 26 of 35 extracts were toxic in all cells with $IC_{50}$ values of $0.1{\sim}2{\mu}g/{\mu}l$. Three extracts including the fruit extracts of Solanum nigrum and Morus alba had no cytotoxic activity in both cell lines. Five of 35 extracts were highly toxic in cancer cells than in primary cells. Because primary cells were more resistant on these extracts, the five extracts were selected for anti-cancer agent candidates. Apoptosis or programmed cell death has an essential role in chemotherapy-induced tumor cell killing. Recently, inducers of apoptosis have been used in cancer therapy. When two of 5 cancer cell-specific cytotoxic extracts (Ulmus parvifolia and Zelkova serrata) were treated in concentration of $0.02{\sim}0.1{\mu}g/{\mu}l$, apoptosis were increased at 3-5 times in cancer cell lines. Finally, the apoptotic effects of these extracts were confirmed by cleavages of both poly-(ADP-ribose)-polymerase and caspase-3 as apoptotic markers. In this report, we suggested that two of 35 medicinal herb extracts can be useful anti-cancer drug candidates inducing apoptosis in several carcinoma cell lines.
Keywords
Ulmus parvifolia; Zelkova serrata; Cytotoxicity; Apoptosis; Anti-cancer;
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Times Cited By KSCI : 4  (Citation Analysis)
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