• Journal of Applied Biological Chemistry
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• 제50권3호
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• pp.164-169
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• 2007

Three coniferous essential oils were extracted from Abies koreana, Cryptomeria japonica, and Torreya nucifera by hydrodistillation. The chemical composition of each oil was analyzed by GCMS, and their antimicrobial activities were tested against two bacteria and one yeast strains. Fortyseven compounds were identified from A. koreana oil, 39 from C. japonica, and 59 from T. nucifera. Main components of the essential oils were limonene (23.5%), bornyl acetate (17.9%), ${\alpha}-pinene$ (11.1%), and camphene (10.2%) in A. koreana, kaurene (26.3%), ${\gamma}-eudesmol$ (19.0%), elemol (6.9%), and sabinene (5.1%) in C. japonica, limonene (13.5%), ${\delta}-cadinene$ (10.5%), ${\alpha}-bisabolol$ (10.2%), and ${\alpha}-copaene$ (7.7%) in T. nucifera. Among the three coniferous trees tested, the essential oils of A. koreana exhibited higher and broader antimicrobial activity against the tested organisms than those of C. japonica and T. nucifera.

• 기술혁신학회지
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• 제16권3호
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• pp.839-860
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• 2013

본 연구는 대표적인 지식기반산업인 생명의료산업의 첨단의료복합단지 조성정책을 대상으로 하여 정책형성과정을 분석하고자 한다. 첨단의료복합단지는 다양한 분석요소와 속성들을 내포하고 있기 때문에 국내외 정치적 경제적 사회적 환경변화에 따른 정책형성과정을 Kingdon(1984, 1995, 2003)의 '정책흐름모형'을 적용하여 분석하였다. 2005년 8월 의료산업선진화위원회가 출범한 시기에서 2008년 3월 '첨단의료복합단지특별법'이 제정되기까지 정책형성 과정을 대상으로 하였다. 분석결과 첨단 의료산업분야의 정책형성 과정은 여러 요인들과 정책흐름들이 간여되어 있으며, 특히 정책의제가 정부의제로 공식화되는 법제화의 과정은 정책선도자 뿐만 아니라 다양한 이해관계자의 역동적인 역할에 의하여 진행된다는 점을 조명하였다.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.603-609
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• 2002

DFA IV is di-D-fructose-2,6':6,2'-dianhydride, consisting of two fructose residues. It can be enzymatically synthesized from levan by levan fructotransferase, and can be used for mineral absorption. Understanding of the structure and composition of levan is important to obtain high-level production of DFA IV. A bacterial strain, Pseudomonas aurantiaca 5-4380, was identified to produce low-branched levan, and the levansucrase gene (lsch) from this bacterium was found to be composed of 1,275 Up coding for a protein of 424 amino acids, with an estimated molecular weight of 47 kDa. The bacterial levansucrase gene was expressed in Escherichia coli DH5${\alpha}$ by its own promoter and lac promoter. The recombinant levansucrase was produced in soluble form with 170U of levansucrase activity from 1-ml E. coii culture broth. The expressed enzyme from the clone showed similar biochemical properties, such as size of active levansucrase, degree of branching, and optimum temperature, with P.aurantiaca 5-4380 levansucrase.

• 기술경영경제학회:학술대회논문집
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• 기술경영경제학회 2002년도 제21회 하계학술발표회 논문집
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• pp.157-179
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• 2002

Many advanced scholars and policy makers say that biotechnology is and will be the most important technology in 21th century. And biotechnology has a lots of character which are new and high tech industry, costing too much on early R&D stage and so on. One of them is that it can't be developed all seater by one single firm. So effective and efficient way to solve this generic problem is using the infrastructure which is given by government for flourishing the bio industry, especially, Knowledge infrastructure. The purpose of this study is to understand and give the information about which knowledge infrastructure affects biotechnology firms to obtain competitive advantage in technological improvement. To develop this object, I made 5 variables about infrastructure in biotechnology such as high-skilled researchers infrastructure, R&D policy infrastructure, industrialization infrastructure that supports the biotechnology industry, networking infrastructure between firms, universities, research centers, and finally bio-safety infrastructure. According to this study, to obtain technological competitive advantage for Korean bio firms, all main actors those are government, industry, university, research center they try to develop bio sector at the same time. After this cooperated effort, Korean bio firms and industry can get a international competitiveness in the global society which no one conquer the market.

• 한국축산식품학회지
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• 제32권1호
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• pp.31-39
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• 2012

The purpose of this study was to isolate bacteriocin-producing bacteria with antagonistic activities against pathogens from the intestines of pigs for probiotic use. Lactobacillus sp. AP 116 possessing antimicrobial property was selected from a total of 500 isolates. The AP 116 strain showed a relatively broad spectrum of inhibitory activity against Listeria monocytogenes, Clostridium perfringens, Pediococcus dextrinicus, and Enterococcus strains using the spot-on-lawn method. Bacteriocin activity remained unchanged after 15 min of heat treatment at $121^{\circ}C$ and exposure to organic solvents; however, it diminished after treatment with proteolytic enzymes. Maximum production of bacteriocin occurred at $34^{\circ}C$ when a pH of 6.0 was maintained throughout the culture during fermentation. According to a tricine SDS-PAGE analysis, the molecular weight of the bacteriocin was approximately 5 kDa. The isolate tolerated bile salts and low pH, and also induced nitric oxide (NO) in mouse peritoneal macrophages. Bacteriocin and bacteriocin-producing bacteria, such as Lactobacillus sp. AP 116, could be potential candidates for use as probiotics as an alternative to antibiotics in the pig industry.

• Journal of Applied Biological Chemistry
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• 제50권3호
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• pp.148-154
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• 2007

Volatile components of the essential oils of Satsuma mandarin (C. unshiu), Dangyuza (C. grandis), Yuza (C. junos), Byungkyul (C. playtymamma), Jinkyul (C. sunki), and Hakyul (C. natsudaidai) grown in Jeju Island were isolated from the fruit peels by hydro distillation and determined by GC-MS. GC-MS analysis identified 58 compounds, with main components being d-limonene $(64.01{\sim}79.34%),\;{\beta}-myrcene\;(3.01{\sim}26.53%),\;{\gamma}-terpinene\;(0.11{\sim}12.88%),\;{\beta}-pinene\;(0.78{\sim}4.74%),\;and\;{\alpha}-pinene\;(1.01{\sim}2.55%)$. Differences in compositions and contents of the essential oils were observed among citrus varieties. Effects of citrus oils on growth inhibitions of Escherchia coli, Staphyllococcus epidermidis, and Candida albicans were investigated using disc diffusion assay and minimal inhibitory concentration (MIC) assay. The essential oils inhibited growths of the test organisms, exhibiting higher levels of activity against Gram-positive S. epidermidis (MIC values $0.04{\sim}0.17mg/mL$), whereas Gram-negative E. coli was moderately resistant (MIC values $1.66{\sim}20.30mg/mL$). MIC of citrus essential oils ranged from $0.82{\sim}23.69mg/mL$ against C. albicans. The essential oils obtained from C. sunki, C. grandis, and C. playtymamma showed the highest antimicrobial activities against S. epidermidis and C. albicans, indicating their potential as natural antimicrobial agents.

• 기술혁신학회지
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• 제13권4호
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• pp.758-771
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• 2010

우리나라는 GDP 대비 R&D투입율이 세계최고의 수준에 있으나 성과는 이에 비해 저조한 실정이다. 본 연구의 목적은 국내 바이오산업에서 전략적 자원과 공동체 공학(community engineering) 이 R&D 성과에 미치는 영향을 분석하는 것이다. 본 연구에서는 성과를 기술적 성과와 경제적 성과로 구분하였으며 조사 분석 결과 전략적 자원과 공동체 공학은 기술적 성과의 결정변수로 나타났으나 경제적 성과에 대해서는 전략적 자원만이 통계적으로 유의미한 영향을 미치는 것으로 나타났다. 또한 전략적 자원이 공동체 공학과 연구 성과 간의 관계에 대한 조절효과를 나타내지 못하는 것으로 나타났다.

• 한국식품과학회지
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• 제45권1호
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• pp.13-19
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• 2013

반응표면분석법을 이용하여 적양배추 종자의 전자선 처리조건과 재배조건에 따른 새싹채소의 이화학적 및 관능적 품질특성을 분석하여 유통에 적합한 최적조건을 모색하였다. 수분함량의 경우 회귀식의 $R^2$가 0.9638이었고 조사선량 및 재배기간에 많은 영향을 받고 있었다. Total phenolics 함량은 회귀식의 $R^2$가 0.9117이었고 조사선량에 많은 영향을 받았으며, carotenoid 함량($R^2$=0.8338)의 경우 조사선량, 재배기간, 저장기간의 순으로 조건 변수의 영향력이 작용하였다. 관능적 품질 역시 전자선 조사선량에 가장 크게 영향을 받아 선량의 증가에 따라 관능평점이 감소하는 경향을 보여주었다. 재배 및 저장조건의 최적화를 위해 total phenolics 함량 및 전반적 기호도를 superimposing한 결과 최적조건은 조사선량 2.2-3.8 kGy, 재배기간 3.0-4.0일 및 저장기간 2.0-3.0일 범위로 예측되었다.

• 한국산업융합학회 논문집
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• 제21권5호
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• pp.197-205
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• 2018

In this research, tricycle vehicle simulation based on multi-body environment has been introduced. Mathematical model of tricycle vehicle was developed. In this research the left and right wheel speed are calculated based on the rear steering angle and velocity. The kinematic model for the three - wheel drive system was completed and the results were analyzed using the actual vehicle drawings. Through simulink vehicle performance on linear and rotation movement were simulated. Using the mathematical model the control system can be applied directly to the tricycle vehicle. The simulation result shows that the proposed vehicle model is successfully represent the movement characteristics of the real vehicle. This model assists the vehicle developer to create the controller and understand the vehicle during the development process.

• 미생물학회지
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• 제54권3호
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• pp.293-294
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• 2018

유류 누출 사고로 오염된 해양 갯벌에서 분리된 Yangia sp. TSB01은 인돌을 인디고로 생물전환 할 수 있는 특성이 있다. 미생물에서 인디고는 산화효소에 의해 인돌로부터 인독실을 거쳐 생성된다. Yangia sp. TSBP01의 유전체는 2개의 chromosome과 5개의 plasmid로 구성되어 있으며, 총 5,165,974 bp의 크기에 G + C 함량은 66.5%이다. TSBP01는 인디고를 생성하는데 관여하는 indole oxygenase를 보유하고 있다.