• Journal of Bio-Environment Control
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• v.26 no.2
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• pp.123-132
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• 2017

This study was aimed to investigate the effect of 1)irradiation with several different ratios using red, green, and blue LEDs and 2)various pulsed light irradiation with 50% duty ratio using red and blue LEDs on the growth and morphogenesis of three lettuce cultivars (Lactuca sativar L. cv. 'Jukchukmeon', 'Lolo Rosa', and 'Grand Rapid') in hydroponics culture system for 4 weeks after transplanting. Seeds were sown in rock-wool plug trays and they were placed in a culture room which was controlled at $23{\pm}1^{\circ}C/18{\pm}1^{\circ}C$ temperature and 50-60/70-85% for day and night, respectively, during cultivation period. Irradiated RGB ratios with LEDs were 6:3:1, 5:2.5:2.5, 3:3:4, 2:2:6, and 1:1:8 with $110{\pm}3{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD on the surface of cultivation bed. The frequencies of pulsed lighting was 50, 100, 500, 1,000, 5,000, 10,000, 25,000Hz (20, 10, 0.1, 0.04 ms) with red and blue LEDs and 50% duty ratio. At the RGB ratio of 6:3:1, the average fresh weight of 'Jukchukmeon' was significantly higher than that of other RGB treatments, but no significant difference compared to the fluorescent treatment. The average fresh weight at 1:1:8 RGB ratio in 'Lolo Rosa' was significantly lower than that of other RGB treatments. Leaf number and fresh weight of 'Grand Rapid' were significantly lower in the control and 1:1:8 RGB treatments, compared to the other RGB treatments. As the ratio of blue light increased, leaf length decreased and leaf shape became round in three lettuces. Although there is little change in growth, it could not be found any tendency to affect the growth and morphogenesis of three lettuces caused by increasing or decreasing frequency of pulsed lighting with 50% duty ratio at the $72{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD.

• Journal of Bio-Environment Control
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• v.26 no.2
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• pp.87-99
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• 2017

Recently the increase in an abnormal climate events and meteorological disasters which causes a great damage to greenhouse facilities. To minimize and evaluate the expected damages it is necessary to prepare countermeasures and a management system in advance. For this purpose, a quantitative analysis of weather and abnormal climate are needed to investigate protected cultivation areas which are vulnerable to natural disasters. This study focused on protected cultivation areas in Jeolla province, South Korea. Surrogate variables were calculated to analyze the vulnerable areas to meteorological disasters, and spatial distribution analysis was also performed by using GIS to present vulnerable areas on map. The map thus created and was compared with actual data of damages by meteorological disasters which occurred in target areas. The result of the comparison is as follows: About 50% of the target areas showed an agreement between the map created in this study and the actual data, these areas includes Gwangju metropolitan city, Naju city, Yeongam County, Jangseong County, Hampyeong County, and Haenam County. On the other hand, other areas, including Gunsan city, Mokpo city, and Muan County, suffered low damage in spite of high levels of vulnerability to meteorological disasters. This result was considered to be affected by such variables as different structural designs and management systems of greenhouses by region. This study carried out an analysis of meteorological data to find out more detailed vulnerability to protected cultivation area and to create a map of vulnerable protected cultivation areas. In addition, the map was compared with the record of natural disasters to identify actual vulnerable areas. In conclusion, this study can be utilized as basic data for preventing and reducing damages by meteorological disasters in terms of design and management of greenhouses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.1
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• pp.9-16
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• 2016

To investigate the effect of soluble silicate zeolite dressing of the rice against bakanae disease, field trial in reclaimed land and in vitro were carried out. The coated rice seeds (SCS) which were dressed with the mixture of 25% silicic acids (binder), and the zeolite (coating powder). In wet direct seeding, uniform scattering of rice seeds on the soil surface and the better seedling establishment were shown in SCS treatment plots. The incidence of bakanae disease began from the mid tillering stage toward the heading stage. Around heading stage, the ratio of infected tillers reached its highest point by 9.9% in non-SCS treatment plots. While, in SCS treatment plots, the ratio of infected tillers was no more than 0.01%. The vitality of the pathogenic fungi of bakanae disease in the SCS and non-SCS samples were assessed. Samples were incubated for one week keeping proper humidity at $30^{\circ}C$ after inoculated with panicles of infected rice plants from experimental field plots. In non-SCS treatment, pinkish colonies were formed on the grain surface of panicle of infected plants, and mycelium, macro-conidia and micro-conidia were developed actively inside part of infected grain inoculated. While in SCS treatment, micro-conidia and mycelium were not survived and the growth of macro-conidia, mycelia were greatly inhibited and withered. Based on the results, it is concluded that the environmental friendly control of bakanae disease by use of SCS is possible and soluble silicate can be applied as agents for replacement of seed disinfection.

• Journal of Bio-Environment Control
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• v.9 no.3
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• pp.171-178
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• 2000

Gross photosynthetic rats of leaves of hydroponically grown cucumber plants(Cucumis sativus L. cv. Guwoosalichungjang) were measured under various conditions of photosynthetic photon flux(PPF), ambient $CO_2$ concentration, air temperature and leaf nitrogen contents. Light compensation point of leaf photosynthesis appeared to be in the range of 10~20$\mu$mol.m$^{-2}$ .s$^{-1}$ and light saturation point be above 1000$\mu$mol.m$^{-2}$ .s$^{-1}$ . Gross photosynthetic rates increased persistently and asymptotically as air temperature rose from 12$^{\circ}C$ to 32$^{\circ}C$. However, there were only small differences in gross photosynthetic rates in the range of 24-32$^{\circ}C$, so that the range seemed to be optimal for photosynthesis of cucumber plants at the condition of $CO_2$ concentration of 400$\mu$mol.mol$^{-1}$ and PPF of around 400$\mu$mol.m$^{-2}$ .s$^{-1}$ . $CO_2$ compensation point of leaf photosynthesis appeared to be in the range of 20-40$\mu$mol.mol$^{-1}$ and $CO_2$ saturation point be above 1200$\mu$mol.mol$^{-1}$ . Gross photosynthetic rates increased sigmoidally as leaf nitrogen content increased. These environmental factors interacted synergistically to enhance gross photosynthetic rate, so that the rate increased multiplicatively s level of one factor increased progressively with higher levels of he other factors. Mathematical models wer developed to estimate the gross photosynthetic rate in accordance with the variations of these environmental factors. These modes can be used not only to explain he variation of growth or yield of cucumber plants under different environmental conditions but also as building blocks of plant growth model or expert system of cucumber plants.

• Korean Journal of Ecology and Environment
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• v.43 no.2
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• pp.263-270
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• 2010

This is a research on development of water purification equipment called artificial floating island (=AFI) for the stagnant water area which can secure exuberant landscape and water-friendility. The equipment devised in this study is designed to make up the weakness of conventional AFIs and improves the removal efficiency of pollutants using the mixture of media and plants. The air compressor positioned at the inlet releases air with inflow continuously, the water pump at the outlet sprays as a form of fountain with causing a disturbance on stable water column, then, both of them contribute improvement of water quality over a large area. We applied Bio-stone as a media in this system and performed an experiment of pre-efficiency test, and we concluded that the higher pollutants concentration of inflow, the higher removal efficiency we obtained. At the result of lab-scale experiment, in the case of high-concentration inflow, in the removal efficiency of SS is 62.2%, BOD is 50.2%, COD is 55.1%, T-N is 31.6%, T-P is 38.4%. In addition, to evaluate the field application, we set up the facilities in Sin-gal lake located in Yongin-Si Gyeonggi-Do, and researched on the removal efficiency of outflow relative to the inflow. As a result, SS is 53.5%, BOD is 32.8%, COD is 36.9%, T-N is 22.6%, T-N is 33.2%.

• Journal of the Korean Magnetics Society
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• v.15 no.2
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• pp.100-105
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• 2005

$Ni_{0.9}Zn_{0.1}Fe_2O_4$ nanoparticles have been prepared by a sol-gel method. The structural and magnetic properties have been investigated by DTA/TGA, XRD, SEM, and $M\ddot{o}ssbauer$ spectroscopy, VSM. $Ni_{0.9}Zn_{0.1}Fe_2O_4$ powder that was annealed at $300^{\circ}C$ has spinel structure and behaved superparamagnetically. The estimated size of superparammagnetic Ni-Zn ferrite nanoparticle is around 10 nm. The hyperfine fields at 13 K for the A and B patterns were found to be 533 and 507 kOe, respectively. The blocking temperature ($T_B$) of superparammagnetic $Ni_{0.9}Zn_{0.1}Fe_2O_4$ nanoparticle is about 250 K. The magnetic anisotropy constant and relaxation time constant of $Ni_{0.9}Zn_{0.1}Fe_2O_4$ nanoparticle were calculated to be $1.6\times10^6\;ergs/cm^3$ and ${\tau}_0=5.0{\times}10^{-13}$ s, respectively. Also, Temperature increased up to $43^{\circ}C$ within 10 minutes under AC magnetic field of 7 MHz. It is considered that $Ni_{0.9}Zn_{0.1}Fe_2O_4$ powder that was annealed at $300^{\circ}C$ is available for biomedicine application such as hyperthermia, drug delivery system and contrast agents in MRI.

• Microbiology and Biotechnology Letters
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• v.35 no.2
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• pp.163-172
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• 2007

This study was designed to investigate that inorganic germanium $(GeO_2)$ did not exist in germanium-fortified yeast or obtained to non-detectable value by current analytical methods and equipments. For this purpose, we achieved $GeO_2$ qualitative analysis protocol which could be the scientific basis of the study. Since reddish brown precipitate was formed from the reaction of $GeO_2$ with 1 equiv $NaBH_4$, and dark brown precipitate was also formed from the reaction of $GeO_2$ with 2 equiv $NaBH_4$, $GeO_2$ was qualitatively analyzed by observing these particular colored-precipitates. Because no color change was showed from the reaction between $NaBH_4$ and $SiO_2$, the color change could be caused by charge transfer transition on Ge-O and B binding properties. The reaction between $NaBH_4$ and germanium-fortified yeast did not show any color change and precipitate formation which meant no $GeO_2$ existed in germanium-fortified yeast. The reaction between $NaBH_4$ and supernatant specimen collected from the outside of dialysis membrane (MWCO 1,200 dalton) did not show any color change and precipitate formation. Therefore, we considered that the both germaniums in and outside of the dialysis membrane were organic germaniums. Germanium-fortified yeast which was biosynthesized organic germanium can be applied not only as a new functional material for improving health, prevention and treatment of chronic degenerative diseases including cancers, and the regulation of immune system, but also as a new materials.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.9
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• pp.1333-1339
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• 2011

After mulberry (Morus alba) leaves were fermented with Hericium erinaceum mycelium by solid-state culture to enhance physiological activity, fermented mulberry leaves (MA-HE) was extracted by hot-water (MA-HEHW) and ethanol (MA-HE-E). MA-HE-HW showed enhanced mitogenic and intestinal immune system modulating activities (1.41 and 1.52 fold of saline control, respectively) compared to hot-water extracts of non-fermented mulberry leaves (MA-HW) and H. erinaceum mycelium (HE-HW) at $100\;{\mu}g$/mL. Meanwhile, when we tested the inhibitory effects of extracts on nitric oxide (NO), tumor necrosis factor (TNF)-${\alpha}$, and interleukin (IL)-$1{\beta}$ and IL-6 production, MA-HE-E significantly inhibited these pro-inflammatory mediators in LPS-stimulated RAW 264.7 cells (45.1, 41.3, 70.2, and 55.7% inhibition of LPS control at $1,000\;{\mu}g$/mL). In addition, MA-HE-HW and MA-HE-E did not show any cytotoxicity on RAW 264.7 cells at $1,000\;{\mu}g$/mL whereas HE-E and MA-E indicated cytotoxicity (80.1 and 30.7% cell viability of saline control). These results suggest that mulberry leaves fermented with H. erinaceum by solid-state culture might have enhanced immunomodulatory and anti-inflammatory effects compared to non-fermented mulberry leaves, resulting in ingredients biotransformed for fermentation with H. erinaceum mycelium.

• Korean Journal of Environment and Ecology
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• v.21 no.3
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• pp.243-256
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• 2007

The purpose of this paper is aimed at identifying the planting condition of greenbelt axis covering forests located at Sinhyeon-eup, Geoje-si and also establishing improvement plans for the ecological organization. The study was executed with buffer green space designed to mitigate noise, which is located at a halfway point linking Mt. Yukkyo(altitude of 50m) and Mt. Jungmae(altitude of 131m). The number of the biotope patterns was classified into 17 in total: the two are urbanized districts such as a townified district and streets and another 15 are greenbelts and open space such as forest biotope, inland water biotope, and landscaping tree plantations biotope. According to the analysis of biotope types, it was estimated that the making use of already established buffer greens as a linking medium with a foothold of Mt. Yukkyo and Jungmae, whose natural eco-system is well suited for habitation of living organism, is the one and only way to the influx of living organism into the downtown area. The green coverage rate of the base green area, sub-base green area and linkage green area was 160.29%, 128.37% and 44.37% respectively; the green capacity coefficient(i.e. GVZ[$Gr{\ddot{u}}nvolumezahl$]) for base green area, sub-base green area and linkage green area was $4.04m^3/m^2,\;3.95m^3/m^2\;and\;0.65m^3/m^2$ respectively. The base green area has constituted multi-layered vegetation structure and thus played a role as habitats for living organism and supply centre of species, whereas the sub-base green area has destroyed lower layer vegetation, and the linkage green area was in bad shape due to the lack of planting volume and damage of the shrub layer. Accordingly, this research paper intended to suggest detailed implementation plans for the improvement in landscape for city dwellers' use and relaxation; in other words, this paper focused on ecological build-up for the Influx of wild birds into the downtown area for the promotion of bio-diversity of species through the linkage of base green areas and the fostering of nature observing trails for citizens as well as the connecting of green areas through the build-up of roadside greens to make these green areas to be efficiently used as corridors for the influx of wild birds and bio-organism habitation and for citizens' using space.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.247-252
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• 2011

Electrical conductivity, drainage, and irrigation amount of nutrient solution are important factors for determination of the mixing ratio of fresh and reused nutrient solutions in closed-loop soilless culture. Generally a fixed mixing ratio is applied in commercial scale greenhouses using solar radiation-based irrigation system. Although it ensures continuous supply of fresh nutrient solution in the mixing process, occasional discharge of the drainage is inevitably required. This study was conducted to compare the nutrient replenishing effect under different mixing processes and to investigate appropriate mixing process. For this experiment, a fixed mixing ratio (FR), modifiable mixing ratio (MR), and open-loop (OP) as control were applied. Mixing ratio was determined by a set value of EC for dilution of collected drainage in FR and the set values of 1.0 and $2.0dS{\cdot}m^{-1}$ were used as treatments (FR 1.0 and FR 2.0), respectively. In MR, mixing ratio was determined based on EC and volume of drainage within irrigation volume per event. The volume of drainage stored in the drainage tank tended to increase in FR 1.0. Although such trend was not observed in FR 2.0 and MR, the volume of drainage stored in MR was lower than that in FR 2.0. The ion balance of $Mg^{2+}:K^+:Ca^{2+}$ or $SO^{2-}_4:NO^-_3:PO^{3-}_4$ in the drainage and reused nutrient solution changed within a narrow range regardless of treatment.