• Parasites, Hosts and Diseases
• /
• v.36 no.1
• /
• pp.15-22
• /
• 1998

An in uitro culture technique was established for harvesting Strongwloides venezuelensis free-living infective larvae using a nutrient broth medium as a substitute for rat-feces in polyvinyl culture bags ($10{\;}{\times}{\;}12{\;}cm$). The egg hatch rate V) in sterile saline at different incubation temperatures (X) was expressed as the quadratic function, Y = $-0.192X^2$ + 8.673x - 19.550 (r = 0.901). The highest (100%) egghatch rate was observed at $25^{\circ}C$. A significant difference (p<0.05) in development rate W) of free-living infective larvae was observed between different concentrations of nutrient broth (X) which was highest (20.6%1 in 0.12% nutrient broth concentrations, incubated at $20^{\circ}C$ for 5 days [Y = $-864.032X^2$ + 245.995X- 0.560 (r = 0.875)]. Yields (Y) of infective larvae were observed relatively high when the culture medium was incubated at higher temperatures (X) which peaked at $25^{\circ}C$ (20.0%) than at lower temperatures. $15^{\circ}C$M (10.9%) and $20^{\circ}C$ (18.1%) [Y = $-0.189X^2$ + 8.387x- 72.795 (r = 0.981)]. The period W) required for the development of infective larvae decreased with higher incubation temperatures (X) [Y = $0.035X^2$ - 2.025X + 32.375 (r = 0.995)] The highest yield (19.2%) of infective larvae was obtained from culture bag inoculated with 15.000 eggs than with below and over 15,000 eggs in 0.12% nutrient broth and incubated at $25^{\circ}C$ for 4 days. The newly adapted culture method (from egg to third-stage larva) may be useful as a bio-bar/bioassay system for screening new chemical products, anthelmintics and pesticides, as well as for parasito immunological studies with Strongwloides species.

• The Korean Journal of Blood Transfusion
• /
• v.23 no.2
• /
• pp.127-135
• /
• 2012

Background: Despite modern advances in laboratory automated medicine, work-process in the blood bank is still handled manually. Several automated immunohematological instruments have been developed and are available in the market. The IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA), a fully automated instrument for immunohematology, was recently introduced. In this study, we evaluated the performance of the IH-1000 for ABO/Rh typing and irregular antibody screening. Methods: In October 2011, a total of 373 blood samples for ABO/Rh typing and 303 cases for unexpected antibody screening were collected. The IH-1000 was compared to the manual tube and slide methods for ABO/Rh typing and to the microcolumn agglutination method (DiaMed-ID system) for antibody screening. Results: For ABO/Rh typing, concordance rate was 100%. For unexpected antibody screening, positive results for both column agglutination and IH-1000 were observed in 10 cases (four cases of anti-E and c, three of anti-E, one of anti-D, one of anti-M, and one of anti-Xg) and negative results for both were observed in 289 cases. The concordance rate between IH-1000 and column agglutination was 98.7%. Sensitivity and specificity were 90.9% and 99.3%, respectively. Conclusion: The automated IH-1000 showed good correlation with the manual tube and slide methods and the microcolumn agglutination method for ABO-RhD typing and irregular antibody screening. The IH-1000 can be used for routine pre-transfusion testing in the blood bank.

• Journal of Bio-Environment Control
• /
• v.30 no.4
• /
• pp.367-376
• /
• 2021

Strawberry cultivation in Korea is grown in greenhouse, but most farms manage their water supply using a timer control method based on the experience of growers. The timer control has problems in that it is difficult to consider the weather condition, the growth stage of crops, and the moisture content of the substrate, so that the crops cannot be managed at an optimal level, and the accuracy of cultivation management are lacking. The watering methods using integrated solar irradiance and substrate moisture contents are control systems that provide eco-friendly and precise water supply considering the growth conditions of crops. The purpose of this study was to compare the combined water supply control with integrated solar irradiance and substrate moisture contents and timer control method in hydroponic cultivation of strawberries using coir, and to set the optimal integrated solar irradiance level for complex water supply control. The irrigation system was automatically watered when it reached 100, 150, 250 J·cm^-2 based on the external solar irradiance, and forced irrigation was performed at a substrate moisture content of less than 60% in all treatments. The amount of irrigation at once was 50 mL. The timer treatment was applied as a control. The smaller the level of integrated radiation to start watering, the greater the daily amount of irrigation. Both the fresh weight and dry weight per plant were higher in the complex irrigation control method than the timer control, and the 100 and 150 J·cm^-2 treatment had the highest fresh weight, and the 100 J·cm^-2 treatment showed a significantly higher dry weight. The yield was also significantly higher in the complex control method than in the timer, and the early yield increased as the level of integrated solar irradiance was smaller. The fresh weight of fruit was the lowest in the timer-controlled irrigation. As a result of this study, the possibility of combined control irrigation method using integrated solar irradiance and substrate moisture content was confirmed for precise water supply management of strawberries in hydroponics.

• Journal of Bio-Environment Control
• /
• v.30 no.4
• /
• pp.401-409
• /
• 2021

This study aimed to estimate the photosynthetic capacity of tomato plants grown in a semi-closed greenhouse using temperature response models of plant photosynthesis by calculating the ribulose 1,5-bisphosphate carboxylase/oxygenase maximum carboxylation rate (V_cmax), maximum electron transport rate (J_max), thermal breakdown (high-temperature inhibition), and leaf respiration to predict the optimal conditions of the CO₂-controlled greenhouse, for maximizing the photosynthetic rate. Gas exchange measurements for the A-C_i curve response to CO₂ level with different light intensities {PAR (Photosynthetically Active Radiation) 200µmol·m^-2·s^-1 to 1500µmol·m^-2·s^-1} and leaf temperatures (20℃ to 35℃) were conducted with a portable infrared gas analyzer system. Arrhenius function, net CO₂ assimilation (A_n), thermal breakdown, and daylight leaf respiration (R_d) were also calculated using the modeling equation. Estimated J_max, A_n, Arrhenius function value, and thermal breakdown decreased in response to increased leaf temperature (> 30℃), and the optimum leaf temperature for the estimated J_max was 30℃. The CO₂ saturation point of the fifth leaf from the apical region was reached at 600ppm for 200 and 400µmol·m^-2·s^-1 of PAR, at 800ppm for 600 and 800µmol·m^-2·s^-1 of PAR, at 1000ppm for 1000µmol of PAR, and at 1500ppm for 1200 and 1500µmol·m^-2·s^-1 of PAR levels. The results suggest that the optimal conditions of CO₂ concentration can be determined, using the photosynthetic model equation, to improve the photosynthetic rates of fruit vegetables grown in greenhouses.

• Journal of Bio-Environment Control
• /
• v.28 no.1
• /
• pp.1-8
• /
• 2019

The study aimed to determine effects of light emitting diode (LED) and the ultraviolet radiation (UVA) light of plant factory on plant growth and ascorbic acid content of spinach (Spinacia oleracea cv. Shusiro). Plants were grown in a NFT (Nutrient Film Technique) system for 28 days after transplanting with fluorescent light (FL, control), LEDs and UVA (Blue+UVA (BUV), Red and Blue (R:B(2:1)) + UVA (RBUV), Red+UVA (RUV), White LED (W), Red and Blue (R:B(2:1)), Blue (B), Red (R)) under the same light intensity ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and photoperiod (16/8h = day/night). All the light sources containing the R (R, RB, RUV, and RBUV) showed leaf epinasty symptom at 21 days after transplanting (DAT). Under the RUV treatment, the lengths of leaf and leaf petiole were significantly reduced and the leaf width was increased, lowering the leaf shape index, compared to the R treatment. Under the BUV, however, the lengths of leaf and leaf petiole were increased significantly, and the leaf number was increased compared to B. Under the RBUV treatment, the leaf length was significantly shorter than other treatments, while no significant difference between the RBUV and RB for the fresh and dry weights and leaf area. Dry weights at 28 days after transplanting were significantly higher in the R, RUV and BUV treatments than those in the W and FL. The leaf area was significantly higher under the BUV treatment. The ascorbic acid content of the 28 day-old spinach under the B was significantly higher, followed by the BUV, and significantly lower in FL and R. All the integrated data suggest that the BUV light seems to be the most suitable for growth and quality of hydroponically grown spinach in a plant factory.

• Journal of Bio-Environment Control
• /
• v.31 no.4
• /
• pp.401-408
• /
• 2022

As research on a controlled environment system based on crop growth environment sensing for sustainable production of horticultural crops and its industrial use has been important, research on how to properly utilize soil moisture sensors for outdoor cultivation is being actively conducted. This experiment was conducted to suggest the proper method of utilizing the TEROS 12, an FDR (frequency domain reflectometry) sensor, which is frequently used in industry and research fields, for each orchard soil in three regions in Korea. We collected soils from each orchard where fruit trees were grown, investigated the soil characteristics and soil water retention curve, and compared TEROS 12 sensor calibration equations to correlate the sensor output to the corresponding soil volumetric water content through linear and cubic regressions for each soil sample. The estimated value from the calibration equation provided by the manufacturer was also compared. The soil collected from all three orchards showed different soil characteristics and volumetric water content values by each soil water retention level across the soil samples. In addition, the cubic calibration equation for TEROS 12 sensor showed the highest coefficient of determination higher than 0.95, and the lowest RMSE for all soil samples. When estimating volumetric water contents from TEROS 12 sensor output using the calibration equation provided by the manufacturer, their calculated volumetric water contents were lower than the actual volumetric water contents, with the difference up to 0.09-0.17 m³·m^-3 depending on the soil samples, indicating an appropriate calibration for each soil should be preceded before FDR sensor utilization. Also, there was a difference in the range of soil volumetric water content corresponding to the soil water retention levels across the soil samples, suggesting that the soil water retention information should be required to properly interpret the volumetric water content value of the soil. Moreover, soil with a high content of sand had a relatively narrow range of volumetric water contents for irrigation, thus reducing the accuracy of an FDR sensor measurement. In conclusion, analyzing soil water retention characteristics of the target soil and the soil-specific calibration would be necessary to properly quantify the soil water status and determine their adequate irrigation point using an FDR sensor.

• Journal of Bio-Environment Control
• /
• v.32 no.1
• /
• pp.40-47
• /
• 2023

There are many different types of cultivation in tomatoes for year-round production. One of them, semi-forcing cultivation is characterized by growing seedlings in winter season. If grafted seedlings are used in winter season that energy cost can be reduced, because they have tolerance to cold stress. This study was conducted to analyze the rootstock performance by measuring the growth, yield, and leaf-macronutrient content of cherry tomatoes grown in semi-forcing hydroponics. Three domestic rootstocks 'HSF4', '21LM', '21A701', and a control cultivar 'B-blocking' were grafted onto jujube-shaped cherry tomato (Lycopersicon esculentum L.) commercial cultivar 'Nonari'. The total yield per plant with grafted cherry tomato '21A701' was 3,387g, which was 11%, 22% and 24% higher than the yield with 'B-blocking', non-grafted one and 'HSF4'. The stem diameter of '21A701' was thick with 8.26mm, whereas non-grafted one was thin with 7.23mm at 160 days after transplanting. The flowering position of '21LM' was 34% and 47% higher than the flowering position of 'B-blocking' and non-grafted one at 153 days after transplanting. The NO₃-N concentration in petiole sap of '21LM' was the highest with 1,746mg·L^-1 and non-grafted one and 'HSF4' were the lowest with 1,252mg·L^-1 and 1,245mg·L^-1 at 167 days after transplanting. The results indicated that rootstock/scion combinations in cherry tomatoes can affect the plant growth, yield, and the concentration of different NO₃-N in leaves at the late growth stage. Both '21A701' and '21LM' have vigorous root system, which influence the growth and yield increased.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.17 no.6
• /
• pp.13-24
• /
• 2022

The purpose of this study is to examine whether the existence of related personnel in KOSDAQ technology special listed firms has a signal effect on the market and affects performance when listed. The KOSDAQ technology special listing system is a system introduced to enable future growth by securing financing through corporate public offering based on the technology and marketability of technology-based startups and venture companies. As a result of analyzing 135 special technology companies listed from 2005 to 21 (excluding SPAC mergers and foreign companies) whether or not related personnel affect corporate value and listing period when they are listed, it was analyzed that the presence of related personnel did not significantly affect corporate value or listing period. The same was found in the results of the verification by reducing the scope to related personnel such as public officials and related agencies. However, under certain conditions, significant results were derived from the presence of related personnel on the listing of companies listed in special technology cases. It was found that the presence of related personnel and VC investment had a significant effect on corporate value, and in the case of bio-industry, there was a slight significant effect on the duration of listing. This study is significant in that it systematically analyzed the signal effect of the existence of related personnel for the first time for all 135 companies. In addition, as a result of the analysis, the results suggest that internalized efforts to secure technology and marketability are more important, such as parallel to VC investment, rather than simply recruiting related personnel.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.27 no.7
• /
• pp.380-394
• /
• 2015

This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2014. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) The research works on the thermal and fluid engineering have been reviewed as groups of heat and mass transfer, cooling and heating, and air-conditioning, the flow inside building rooms, and smoke control on fire. Research issues dealing with duct and pipe were reduced, but flows inside building rooms, and smoke controls were newly added in thermal and fluid engineering research area. (2) Research works on heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer and industrial heat exchangers. Researches on heat transfer characteristics included the results for thermal contact resistance measurement of metal interface, a fan coil with an oval-type heat exchanger, fouling characteristics of plate heat exchangers, effect of rib pitch in a two wall divergent channel, semi-empirical analysis in vertical mesoscale tubes, an integrated drying machine, microscale surface wrinkles, brazed plate heat exchangers, numerical analysis in printed circuit heat exchanger. In the area of pool boiling and condensing, non-uniform air flow, PCM applied thermal storage wall system, a new wavy cylindrical shape capsule, and HFC32/HFC152a mixtures on enhanced tubes, were actively studied. In the area of industrial heat exchangers, researches on solar water storage tank, effective design on the inserting part of refrigerator door gasket, impact of different boundary conditions in generating g-function, various construction of SCW type ground heat exchanger and a heat pump for closed cooling water heat recovery were performed. (3) In the field of refrigeration, various studies were carried out in the categories of refrigeration cycle, alternative refrigeration and modelling and controls including energy recoveries from industrial boilers and vehicles, improvement of dehumidification systems, novel defrost systems, fault diagnosis and optimum controls for heat pump systems. It is particularly notable that a substantial number of studies were dedicated for the development of air-conditioning and power recovery systems for electric vehicles in this year. (4) In building mechanical system research fields, seventeen studies were reported for achieving effective design of the mechanical systems, and also for maximizing the energy efficiency of buildings. The topics of the studies included energy performance, HVAC system, ventilation, and renewable energies, piping in the buildings. Proposed designs, performance performance tests using numerical methods and experiments provide useful information and key data which can improve the energy efficiency of the buildings. (5) The field of architectural environment was mostly focused on indoor environment and building energy. The main researches of indoor environment were related to the evaluation of work noise in tunnel construction and the simulation and development of a light-shelf system. The subjects of building energy were worked on the energy saving of office building applied with window blind and phase change material(PCM), a method of existing building energy simulation using energy audit data, the estimation of thermal consumption unit of apartment building and its case studies, dynamic window performance, a writing method of energy consumption report and energy estimation of apartment building using district heating system. The remained studies were related to the improvement of architectural engineering education system for plant engineering industry, estimating cooling and heating degree days for variable base temperature, a prediction method of underground temperature, the comfort control algorithm of car air conditioner, the smoke control performance evaluation of high-rise building, evaluation of thermal energy systems of bio safety laboratory and a development of measuring device of solar heat gain coefficient of fenestration system.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.18 no.3
• /
• pp.740-746
• /
• 2004

In order to identify the anti-inflammatory and analgesic properties of natural herbal extracts, widely used in the Korean traditional medicine, an in vitro screening system was designed using pGL3, a luciferase reporter vector, and the tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-II as target genes. The promoter regions of each gene was generated by PCR using the human chromosome as template DNA, and inserted into pGL3 vector with Kpnl and Hindlll. The final construct was transfected into human myleomonocytic leukemia cells (U937) that could be differentiated and activated by phorbol 12-myristate 13-acetate (PMA) or lipopolysaccharide (LPS). Using this system, we tested the anti-inflammatory and analgesic effects of several herbal extracts being regarded to have the medicinal effects of diminishing the body heat and complementing Qi. The well-known chemicals of PD98059 and berberine chloride were used as controls of the transcriptional inhibitors of TNF-α and COX-II, respectively. Among them, Salvia miltiorrhiza (Dan-Sam) was found to exhibit the significant medicinal properties of anti-inflammatory and analgesic effects.