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In vitro Study of Anti-inflammatory Effects of Salvia Miltiorrhiza Extracts Using Luciferase Reporter Gene Assay  

Lee Han Chang (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Yeom Mi Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Kim Gun Ho (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Han Dong Oh (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Zhao Mei Ai (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Shim In Sop (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Lee Hye Jung (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Choi Kang Duk (Bio & Information Technology, Hankyong National University)
Hahm Dae Hyun (Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.3, 2004 , pp. 740-746 More about this Journal
Abstract
In order to identify the anti-inflammatory and analgesic properties of natural herbal extracts, widely used in the Korean traditional medicine, an in vitro screening system was designed using pGL3, a luciferase reporter vector, and the tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-II as target genes. The promoter regions of each gene was generated by PCR using the human chromosome as template DNA, and inserted into pGL3 vector with Kpnl and Hindlll. The final construct was transfected into human myleomonocytic leukemia cells (U937) that could be differentiated and activated by phorbol 12-myristate 13-acetate (PMA) or lipopolysaccharide (LPS). Using this system, we tested the anti-inflammatory and analgesic effects of several herbal extracts being regarded to have the medicinal effects of diminishing the body heat and complementing Qi. The well-known chemicals of PD98059 and berberine chloride were used as controls of the transcriptional inhibitors of TNF-α and COX-II, respectively. Among them, Salvia miltiorrhiza (Dan-Sam) was found to exhibit the significant medicinal properties of anti-inflammatory and analgesic effects.
Keywords
Salvia miltiorrhiza(丹蔘); anti-inflammation; analgesia; luciferase; reporter gene; tumor necrosis factor α; cyclooxygenase II; in vitro screening;
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