• Applied Microscopy
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• v.45 no.3
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• pp.150-154
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• 2015

Negative staining electron microscopy facilitates the visualization of small bio-materials such as proteins; thus, many electron microscopists have used this conventional method to visualize the morphologies and structures of biological materials. To achieve sufficient contrast of the materials, a number of imaging parameters must be considered. Here, we examined the effects of one of the fundamental imaging parameters, electron beam exposure time, on electron densities generated using transmission electron microscopy. A single site of a negatively stained biological sample was illuminated with the electron beam for different times (1, 2, or 4 seconds) and sets of micrographs were collected. Computational image processing demonstrated that longer exposure times provide better electron densities at the molecular level. This report describes technical procedures for testing parameters that allow enhanced evaluations of the densities of electron microscopy images.

• Electronics and Telecommunications Trends
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• v.33 no.6
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• pp.81-93
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• 2018

Metamaterials are artificial media that can control the properties of waves at will. Active photonic metadevice technologies cover the device and material technologies that control the visible and IR light through an external signal (mainly an electrical signal). The application areas of active photonic metadevices are tremendous for example holography, active HOE, bio imaging, IR imaging, telecommunication, and optoelectronic devices. In this paper, the technical trends and prospects of active metamaterials, active meta holography, active meta devices, nano-optical telecommunication devices, and IR imaging meta devices are reviewed.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.617-624
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• 2019

Bacterial nanocellulose (BNC) which is generally synthesized by several species of bacteria has a wide variety of industrial uses, particularly in the food and material industries. However, the low levels of BNC production during the fermentation process should be overcome to reduce its production cost. Therefore, in this study, we screened and identified a new cellulose-producing bacterium, optimized production of the cellulose, and investigated the morphological properties of the cellulosic materials. Out of 147 bacterial isolates from ripened fruits and traditional vinegars, strain SFCB22-18 showed the highest capacity for BNC production and was identified as Komagataeibacter sp. based on 16S rRNA sequence analysis. During 6-week fermentation of the strain using an optimized medium containing 3.0% glucose, 2.5% yeast extract, 0.24% acetic acid, 0.27% $Na_2HPO_4$, and 0.5% ethanol at $30^{\circ}C$, about 5 g/l of cellulosic material was produced. Both imaging and IR analysis proved that the produced cellulose would be nanoscale bacterial cellulose.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.29 no.11
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• pp.685-689
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• 2016

Synthesis of the fluorescent Au nanoclusters is reported. The Au nanoclusters were synthesized via reduction of gold ions in reverse micelles with mild reducing agents. The Au nanoclusters show a bright red emission at 640 nm. The fluorescent Au nanoclusters attract great interest for sensor, electronic device and bio-imaging applications because of ultra-small size, high chemical stablity and bright emission. We believe that the fluorescent Au nanoclusters can have optoelectronic applications such as optical down conversion phosphors.

• Journal of the Optical Society of Korea
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• v.20 no.1
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• pp.78-87
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• 2016

Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC’s membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

• Journal of Bio-Environment Control
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• v.22 no.1
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• pp.73-79
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• 2013

An experiment was conducted to study incidences of heat loss in greenhouse in Gyeongnam province using thermal imaging camera in order to determine ways minimizing greenhouse heat loss. Measurements of this work showed that temperature differences between two experiment zones before and after installation of thermal curtains were about $2.0{\sim}3.0^{\circ}C$ and $1.0{\sim}2.0^{\circ}C$ respectively. There was a high correlation between the temperature data measured using a thermal imaging camera and a temperature sensor. There was no serious difference among areas, but between places on the first and second floor with thermal curtains for heat insulation, there was a relatively larger heat loss on the first floor than the second floor. Then in general the greenhouse types had no particular bearing on this matter, there was a relatively large heat loss in the parts of side wall window, the gaps and the parts folded of horizontal thermal curtains, the gutter parts, and the gaps of thermal curtain in the side wall window and facade back side for heat insulation, aren't completely sealed. It was found that there was a substantial heat loss due to infiltration through cracks on covering material, doors, ventilating openings, roof gables and floors, in particular.

• Journal of the Optical Society of Korea
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• v.19 no.1
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• pp.55-62
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• 2015

In this study we demonstrate ultrahigh-resolution spectral domain optical coherence tomography (UHR SD-OCT) with a linear-wavenumber (k) spectrometer, to accelerate signal processing and to display two-dimensional (2-D) images in real time. First, we performed a numerical simulation to find the optimal parameters for the linear-k spectrometer to achieve ultrahigh axial resolution, such as the number of grooves in a grating, the material for a dispersive prism, and the rotational angle between the grating and the dispersive prism. We found that a grating with 1200 grooves and an F2 equilateral prism at a rotational angle of $26.07^{\circ}$, in combination with a lens of focal length 85.1 mm, are suitable for UHR SD-OCT with the imaging depth range (limited by spectrometer resolution) set at 2.0 mm. As guided by the simulation results, we constructed the linear-k spectrometer needed to implement a UHR SD-OCT. The actual imaging depth range was measured to be approximately 2.1 mm, and axial resolution of $3.8{\mu}m$ in air was achieved, corresponding to $2.8{\mu}m$ in tissue (n = 1.35). The sensitivity was -91 dB with -10 dB roll-off at 1.5 mm depth. We demonstrated a 128.2 fps acquisition rate for OCT images with 800 lines/frame, by taking advantage of NVIDIA's compute unified device architecture (CUDA) technology, which allowed for real-time signal processing compatible with the speed of the spectrometer's data acquisition.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.140.2-140.2
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• 2016

Despite the growing interest in GeTe as a archetypal displacive ferroelectric material as well as the basis of related materials used in data-storage applications, atom-resolved study of single crystalline GeTe surface been lacking. Using low temperature scanning tunneling microscopy (STM) and spectroscopy (STS), we investigated as-cleaved and annealed surfaces of GeTe. We found that as-cleaved GeTe(111) surface is composed of at least two kinds of terraces at 78 K. While two terraces show metallic characteristics, they also exhibit distinctive I-V spectra and imaging conditions, with each being attributed to Ge-terminated, and Te-terminated surfaces respectively. GeTe(111) surfaces annealed at moderately elevated temperature introduces intricate networks of extended defect structures. We will present these data and discuss the role of vacancies in the formation of these structures.

• The Research Journal of the Costume Culture
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• v.31 no.1
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• pp.107-123
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• 2023

This research studied the electrical characteristics, IR transmission characteristics, stealth functions, and thermal characteristics of infrared thermal-imaging cameras of copper-sputtered samples. Nylon samples were prepared for each density as a base material for copper-sputtering treatment. Copper-sputtered NFi, NM1, NM2, NM3, NM4, and NM5, showed electrical resistance of 0.8, 445.7, 80.7, 29.7, 0.3, and 2.2 Ω, respectively, all of which are very low values; for the mesh sample, the lower the density, the lower the electrical resistance. Measuring the IR transmittance showed that the infrared transmittance of the copper-sputtered samples was significantly reduced compared to the untreated sample. Compared to the untreated samples, the transmittance went from 92.0-64.1%. When copper sputtered surface was directed to the IR irradiator, the IR transmittance went from 73.5 to 43.8%. As the density of the sample increased, the transmittance tended to decreased. After the infrared thermal imaging, the absolute values of △R, △G, and △B of the copper phase increased from 2 to 167, 98 to 192, and 7 to 118, respectively, and the closer the density of the sample (NM5→NFi), the larger the absolute value. This proves that the dense copper phase-up sample has a stealth effect on the infrared thermal imaging camera. It is believed that the copper-sputtered nylon samples produced in this study have applications in multifunctional uniforms, bio-signal detection sensors, stage costumes, etc.

• Journal of radiological science and technology
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• v.39 no.1
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• pp.35-42
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• 2016

Room-temperature operating CdZnTe(CZT) material is an innovative radiation detector which could reduce the patient dose to one-tenth level of conventional CT (Computed Tomography) and mammography system. The pixel and pixel pitch in the imaging device determine the conversion efficiency of incident X-or gamma-ray and the cross-talk of signal, that is, image quality of detector system. The weighting potential is the virtual potential determined by the position and geometry of electrode. The weighting potential obtained by computer-based simulation in solving Poisson equation with proper boundaries condition. The pixel was optimized by considering the CIE (charge induced efficiency) and the signal cross-talk in CT detector system. The pixel pitch was 1-mm and the detector thickness was 2-mm in the simulation. The optimized pixel size and inter-pixel distance for maximizing the CIE and minimizing the signal cross-talk is about $750{\mu}m$ and $125{\mu}m$, respectively.