• JSTS:Journal of Semiconductor Technology and Science
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• 제7권3호
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• pp.151-160
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• 2007

We report the label-free biomolecules detection based on nanomechanical micro cantilevers operated in dynamic mode for detection of two marker proteins (myoglobin and creatin kinase-MB (CK-MB)) of acute myocardical infarctions. When the specific binding between the antigen and its antibody occurred on the fuctionalized microcantilever surface, mechanical response (i.e. resonant frequency) of microcantilevers was changed in lower frequency range. We performed the label-free biomolecules detection of myoglobin and CK-MB antigen in the low concentration (clinical threshold concentration range) as much as 1 ng/ml from measuring the dynamic response change of micro cantilevers caused by the intermolecular force. Moreover, we estimate the surface stress on the dynamic microcantilevers generated by specific antibody-antigen binding. It is suggested that our dynamic microcantilevers may enable one to use the sensitive label-free biomolecules detection for application to the disease diagnosis system based on mechanical immuno-sensor.

• 분석과학
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• 제25권3호
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• pp.190-196
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• 2012

납에 의한 노출에 대한 평가에는 주로 전혈 중 납을 사용하고 있으나 전혈 중 납은 납의 단기 노출에 대한 정보만을 제공한다는 단점이 있다. 납 노출의 만성적 노출지표인 혈장 중 납을 분석하기 위해서는 수 ng/L의 극미량 분석이 요구되어 납의 만성지표로서의 효용성 검증에 어려움이 있다. 본 연구에서는 납 노출 근로자의 만성 노출 지표로서 혈장 중 납을 분석하기 위하여 유도결합플라즈마 질량분석법을 정립하였다. 외부환경으로부터의 오염을 최소화시키기 위해 class 1,000 수준의 청정실을 설치한 후 가동 전과 후의 부유 분진량을 확인한 후 극미량 시료 분석을 수행하였다. 표준 우태아 혈청을 이용하여 표준물 첨가법에 의한 표준편차를 이용하여 계산한 최소검출한계는 4.3 ng/L, 최소정량한계는 12.2 ng/L이었으며, 신호-대-잡음 비로 계산한 최소검출한계는 7.0 ng/L, 최소정량한계는 22.1 ng/L이었다. 20 ng/L부터 2,000 ng/L 농도 범위에서 정밀도는 4% 이내였으며, 우태아 혈청에 20-2,000 ng/L의 농도로 첨가하여 계산한 회수율은 92.3-101.3%로 양호한 결과를 얻었다. 본 연구에서 제시한 방법을 사용하여 납 노출근로자의 혈장 및 혈청 중 납 분석이 가능하였으며 이를 통해 납의 만성노출에 대한 조사가 가능할 것으로 판단된다.

• 한국어병학회지
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• 제26권3호
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• pp.241-254
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• 2013

본 연구는 환경내의 내분비교란화학물질 조사를 위한 바이오마커 시스템을 개발하기 위하여 쏨뱅이의 Vitellogenin(Vtg)을 바이오마커로서 활용하기 위하여 실험을 실시하였다. Vtg는 에스트로겐을 이용하여 유도하였으며, 어류의 혈청으로부터 겔 여과 및 이온교환크로마토그래피를 이용하여 정제하였다. 그 후 BALB/C 마우스를 이용하여 정제된 Vtg의 하이브리도마를 생산하여 항-Vtg의 항체를 생산하였다. 쏨뱅이로부터 유되된 Vtg의 크기는 Sepharose CL-6B을 이용한 겔 여과를 통하여 약 440 kDa 인 것으로 나타났으며 main-band는 175 kDa이었으며 몇 개의 sub-band가 발견되어졌다. 8개의 단클론 항체가 개발한 하이브리도마로부터 얻을 수 있었으며, 이 항체는 본 실험에서 조사한 어류 중 우럭볼락 Sebastes hubbsi을 제외한 다른 종에서는 교차반응이 이루어지지 않았다. 그 결과, 클론 S28과 S15의 단클론항체를 ELISA와 ICG assay의 추적 인자로 사용할 수 있을 것으로 판단된다. 본 연구에서 배발된 검출시스템은 다양한 내분비교란 활동의 확인뿐 만이 아니라 내분비교란화학물질로 알려진 물질을 검출하는 바이오 마커 분석에 활용될 수 있을 것이다.

• The Plant Pathology Journal
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• 제29권2호
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• pp.144-153
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• 2013

Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

• 디지털콘텐츠학회 논문지
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• 제17권6호
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• pp.509-521
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• 2016

본 논문에서는 파킨슨 병 진단 및 바이오 표지자 검출을 위한 극한 기계학습을 결합하는 새로운 균형 표본 유전 알고리즘(SBGA-ELM)을 제안하였다. 접근법은 정확한 파킨슨 병 진단 및 바이오 표지자 검출을 위해 공개 파킨슨 병 데이터베이스로부터 22,283개의 유전자의 발현 데이터를 사용하며 다음의 두 가지 주요 단계를 포함하였다 : 1. 특징(유전자) 선택과 2. 분류단계이다. 특징 선택 단계에서는 제안된 균형 표본 유전 알고리즘에 기반하고 파킨스병 데이터베이스(ParkDB)의 유전자 발현 데이터를 위해 고안되었다. 제안된 제안 된 SBGA는 추가적 분석을 위해 ParkDB에서 활용 가능한 22,283개의 유전자 중에서 강인한 서브셋을 찾는다. 특징분류 단계에서는 정확한 파킨슨 병 진단을 위해 선택된 유전자 세트가 극한 기계학습의 훈련에 사용된다. 발견 된 강인한 유전자 서브세트는 안정된 일반화 성능으로 파킨슨 병 진단을 할 수 있는 ELM 분류기를 생성하게 된다. 제안된 연구에서 강인한 유전자 서브셋은 파킨슨병을 관장할 것으로 예측되는 24개의 바이오 표지자를 발견하는 데도 사용된다. 논문을 통해 발견된 강인 유전자 하위 집합은 SVM이나 PBL-McRBFN과 같은 기존의 파킨슨 병 진단 방법들을 통해 검증되었다. 실시된 두 가지 방법(SVM과 PBL-McRBFN)에 대해 모두 최대 일반화 성능을 나타내었다.

• Journal of Mechanical Science and Technology
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• 제17권6호
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• pp.825-835
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• 2003

Previous studies have demonstrated the importance of joint angle errors mainly due to skin artifact and measurement errors during gait analysis. Joint angle errors lead to unreliable kinematics and kinetic analyses in the investigation of human motion. The purpose of this paper is to present the Joint Averaging Coordinate System (JACS) method for human gait analysis. The JACS method is based on the concept of statistical data reduction of anatomically referenced marker data. Since markers are not attached to rigid bodies, different marker combinations lead to slightly different predictions of joint angles. These different combinations can be averaged in order to provide a "best" estimate of joint angle. Results of a gait analysis are presented using clinically meaningful terminology to provide better communication with clinical personal. In order to verify the developed JACS method, a simple three-dimensional knee joint contact model was developed, employing an absolute coordinate system without using any kinematics constraint in which thigh and shank segments can be derived independently. In the experimental data recovery, the separation and penetration distance of the knee joint is supposed to be zero during one gait cycle if there are no errors in the experimental data. Using the JACS method, the separation and penetration error was reduced compared to well-developed existing methods such as ACRS and Spoor & Veldpaus method. The separation and penetration distance ranged up to 15 mm and 12 mm using the Spoor & Veldpaus and ACRS method, respectively, compared to 9 mm using JACS method. Statistical methods like the JACS can be applied in conjunction with existing techniques that reduce systematic errors in marker location, leading to an improved assessment of human gait.

• 대한본초학회지
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• 제28권5호
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• pp.1-6
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• 2013

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1232-1237
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• 2014

Lycopene, which is a well-known red carotenoid pigment, has been drawing scientific interest because of its potential biological functions. The current study reports that lycopene acts as a bactericidal agent by inducing reactive oxygen species (ROS)-mediated DNA damage in Escherichia coli. Lycopene treatment elevated the level of ROS-in particular, hydroxyl radicals ($^*OH$)-which can damage DNA in E. coli. Lycopene-induced DNA damage in bacteria was confirmed and we also observed cell filamentation caused by cell division arrest, an indirect marker of the DNA damage repair system, in lycopene-treated E. coli. Increased RecA expression was observed, indicating activation of the DNA repair system (SOS response). To summarize, lycopene exerts its antibacterial effects by inducing $^*OH$-mediated DNA damage that cannot be ameliorated by the SOS response. Lycopene may be a clinically useful adjuvant for current antimicrobial therapies.

• 원예과학기술지
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• 제35권2호
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• pp.232-242
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• 2017

The goal of marker-assisted backcrossing (MAB) is to significantly reduce the number of breeding generations required by using genome-based molecular markers to select for a particular trait; however, MAB systems have only been developed for a few vegetable crops to date. Among the types of molecular markers, SNPs (single-nucleotide polymorphisms) are primarily used in the analysis of genetic diversity due to their abundance throughout most genomes. To develop a MAB system in Chinese cabbage, a high-throughput (HT) marker system was used, based on a previously developed set of 468 SNP probes (BraMAB1, Brassica Marker Assisted Backcrossing SNP 1). We selected a broad-spectrum TuMV (Turnip mosaic virus) resistance (trs) Chinese cabbage line (SB22) as a donor plant, constructing a $BC_1F_1$ population by crossing it with the TuMV-susceptible 12mo-682-1 elite line. Foreground selection was performed using the previously developed trsSCAR marker. Background selection was performed using 119 SNP markers that showed clear polymorphism between donor and recipient plants. The background genome recovery rate (% recurrent parent genome recovery; RPG) was good, with three of 75 $BC_1F_1$ plants showing a high RPG rate of over 80%. The background genotyping result and the phenotypic similarity between the recurrent parent and $BC_1F_1$ showed a correlation. The plant with the highest RPG recovery rate was backcrossed to construct the $BC_2F_1$ population. Foreground selection and background selection were performed using 169 $BC_2F_1$ plants. This study shows that, using MAB, we can recover over 90% of the background genome in only two generations, highlighting the MAB system using HT markers as a highly efficient Brassica rapa backcross breeding system. This is the first report of the application of a SNP marker set to the background selection of Chinese cabbage using HT SNP genotyping technology.

• Progress in Superconductivity
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• 제9권1호
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• pp.35-39
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• 2007

Myocardial ischemia causes heterogeneity of ventricular repolarization and sometimes produces changes of the ST-T wave in ECG. Therefore, morphological changes of ST-T waveform in ECG have a clinical significance in diagnosing myocardial ischemia. In this study, we investigated the ST-T changes caused by myocardial ischemia in magnetocardiography (MCG). We analyzed MCG patterns of biphasic T, ST segment deviations from baseline, main current angle of $T_{peak}$ and $T_{peak}$ dispersion in 300 CAD patients without ST elevation in ECG, 122 symptomatic patients and 48 normal subjects. MCGs were recorded by multichannel SQUID system in a magnetically shielded room. As results, we found that appearances of the abnormality were strongly correlated with the severity of myocardial ischemia. Also we found that the percentage of the patients showing MCG changes were higher than those in ECG. These results show that morphological changes of ST-T waveform in MCG can be used as a marker of myocardial ischemia.