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http://dx.doi.org/10.4014/jmb.1406.06009

Lycopene-Induced Hydroxyl Radical Causes Oxidative DNA Damage in Escherichia coli  

Lee, Wonyoung (School of Life Sciences, BK 21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University)
Lee, Dong Gun (School of Life Sciences, BK 21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.9, 2014 , pp. 1232-1237 More about this Journal
Abstract
Lycopene, which is a well-known red carotenoid pigment, has been drawing scientific interest because of its potential biological functions. The current study reports that lycopene acts as a bactericidal agent by inducing reactive oxygen species (ROS)-mediated DNA damage in Escherichia coli. Lycopene treatment elevated the level of ROS-in particular, hydroxyl radicals ($^*OH$)-which can damage DNA in E. coli. Lycopene-induced DNA damage in bacteria was confirmed and we also observed cell filamentation caused by cell division arrest, an indirect marker of the DNA damage repair system, in lycopene-treated E. coli. Increased RecA expression was observed, indicating activation of the DNA repair system (SOS response). To summarize, lycopene exerts its antibacterial effects by inducing $^*OH$-mediated DNA damage that cannot be ameliorated by the SOS response. Lycopene may be a clinically useful adjuvant for current antimicrobial therapies.
Keywords
Lycopene; hydroxyl radical; DNA damage; oxidative stress; Escherichia coli;
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