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http://dx.doi.org/10.5573/JSTS.2007.7.3.151

Quantitative Label-free Biodetection of Acute Disease Related Proteins Based on Nanomechanical Dynamic Microcantilevers  

Hwang, Kyo-Seon (Nano-Bio Research Center, Korea Institute of Science and Technology)
Cha, Byung-Hak (Nano-Bio Research Center, Korea Institute of Science and Technology)
Kim, Sang-Kyung (Nano-Bio Research Center, Korea Institute of Science and Technology)
Park, Jung-Ho (Department of Electronics and Computer Engineering, Korea University)
Kim, Tae-Song (Nano-Bio Research Center, Korea Institute of Science and Technology)
Publication Information
JSTS:Journal of Semiconductor Technology and Science / v.7, no.3, 2007 , pp. 151-160 More about this Journal
Abstract
We report the label-free biomolecules detection based on nanomechanical micro cantilevers operated in dynamic mode for detection of two marker proteins (myoglobin and creatin kinase-MB (CK-MB)) of acute myocardical infarctions. When the specific binding between the antigen and its antibody occurred on the fuctionalized microcantilever surface, mechanical response (i.e. resonant frequency) of microcantilevers was changed in lower frequency range. We performed the label-free biomolecules detection of myoglobin and CK-MB antigen in the low concentration (clinical threshold concentration range) as much as 1 ng/ml from measuring the dynamic response change of micro cantilevers caused by the intermolecular force. Moreover, we estimate the surface stress on the dynamic microcantilevers generated by specific antibody-antigen binding. It is suggested that our dynamic microcantilevers may enable one to use the sensitive label-free biomolecules detection for application to the disease diagnosis system based on mechanical immuno-sensor.
Keywords
Microcantilever; resonant frequency; Myocardial infactions; surface stress; nanmechanics;
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1 H.-F. Ji, T. Thundat, 'In-situ detection of calcium ions with chemically modified microcantilevers,', Biosens. Bioelectron., Vol. 17, pp. 337-343, 2000   DOI   ScienceOn
2 J. Fritz, M.K. Baller, H.P. Lang, H. Rothuizen, P. Vettiger, E. Meyer, H.-J. Guntherodt, C. Gerber, J.K. Gimzewski, 'Translating biomolecular recognition into nanomechanics,' Science, Vol. 288, pp. 316-318, 2000   DOI   ScienceOn
3 G. Wu, R.H. Datar, K.M. Hansen, T. Thundat, R.J. Cote, A. Marjumdar, 'Bioassay of prostate-specific antigen (PSA) using microcantilevers,' Nat. Biotechnol., Vol. 19, pp. 856-860, 2001   DOI   ScienceOn
4 H.G. Hansma, L.I. Pietrasanta, I.D. Auerbach, C. Sorensen, R. Golan, P.A. Holden, 'Probing biopolymers with the atomic force microscope: a review,' J. Biomater. Sci., Polym. Ed. 11, Vol. 7, pp. 675-683, 2000
5 C.A. Savran, S.M. Knudsen, A.D. Ellington, S.R. Manalis, 'Micromechanical detection of proteins using aptamer-based receptor molecules,' Anal. Chem., Vol. 76, pp. 3194-3198, 2004   DOI   ScienceOn
6 B. Ilic. Y. Yang, H.G. Craighead, 'Virus detection using nanoelectromechanical devices,' Appl. Phys. Lett., Vol. 85, pp. 2604-2606, 2004   DOI   ScienceOn
7 Y. Lee, E.K. Lee, Y.W. Cho, T. Matsui, I.-C, Kang, T.-S. Kim, M. H. Han, 'ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies,' Proteomics, Vol. 3, pp. 2289-2304, 2003   DOI   ScienceOn
8 D.W. Chun, K.S. Hwang, K. Eom, J.H. Lee, B.H. Cha, W.Y. Lee. D.S. Yoon, T.S. Kim, 'Detection of the Au thin-layer in Hz per picogram regime based on the microcantilevers,' Sens. Actuat. A Vol. 135, pp. 857-862, 2007   DOI   ScienceOn
9 K.S. Hwang, K. Eom, J.H. Lee, D.W. Chun, B.H. Cha, D.S. Yoon, T.S. Kim, J.H. Park, 'Dominant surface stress driven by biomolecular interactions in the dynamical response of nanomechanical microcantilevers,' Appl. Phys. Lett., Vol. 89, 173905, 2006   DOI   ScienceOn
10 P.E. Marszalek, H. Lu, H. Li, M. Carrion-Vazquez, A.F. Oberhauser, K. Schulten, J.M. Fernandez, 'Mechanical unfolding intermediates in titin modules,' Nature, Vol. 402, pp. 100-103, 1999   DOI   ScienceOn
11 N. Backmann, C. Zahnd, F. Huber. A. Bietsch, A. Pluckthun, H.-P. Lang, H.-J. Guntherodt, M. Hegner, C. Gerber, 'A label-free immunosensor array using single-chain antibody fragments,' Proc. Natl. Acad. Sci. USA., Vol. 102, pp. 14587-14592, 2005
12 J.H. Lee, T.S. Kim, and K.H. Yoon, 'Effect of mass and stress on resonant frequency shift of functionalized Pb(Zr0.52Ti0.48)O3 thin film microcantilever for the detection of C-reactive protein', Appl. Phys. Lett., Vol. 84, pp. 3187-3189, 2004   DOI   ScienceOn
13 Y. Arntz, J.D. Seelig, H.P. Lang, J. Zhang, P. Hunziker, J.P. Ramseyer, E. Meyer, M. Hegner, Ch. Gerber, 'Label-free protein assay based on a nanomechanical cantilever array,' Nanotechnology, Vol. 14, pp. 86-90, 2003   DOI   ScienceOn
14 C. Sander, 'Genomic medicine and the future of health care,' Science, Vol. 287, pp. 1977-1978, 2000   DOI   ScienceOn
15 J.H. Lee, K.S. Hwang, J. Park, K.H. Yoon, D.S. Yoon, and T.S. Kim, 'Immunoassay of prostate specific antigen (PSA) using resonant frequency shift of piezoelectric nanomechanical cantilever,' Biosens. Bioelectron. Vol. 20, pp. 2157-2162, 2005   DOI   ScienceOn
16 T.R. Strick, M.N. Dessinges, G. Charvin, N.H. Dekker, J.-F. Allemand, D. Bensimon, V. Croquette, 'Stretching of macromolecules and proteins,' Rep. Prog. Phys., Vol. 66, pp. 1-45, 2003   DOI   ScienceOn
17 B. Ilic. Y. Yang, K. Aubin. R. Reichenbach, S. Krylov, H.G. Craighead, 'Enumeration of DNA molecules bound. to a nanomechanical oscillator,' Nano. Lett., Vol. 5, pp. 925-929, 2005   DOI   ScienceOn
18 J.H. Lee, K.H. Yoon, K.S. Hwang, J. Park, S. Ahn, and T.S. Kim, 'Label free novel electrical detection using micromachined PZT monolithic thin film cantilever for the detection of C-reactive protein,' Biosens. Bioelectron., Vol. 20, pp. 269-275, 2004   DOI   ScienceOn
19 K.S. Hwang, J.H. Lee, J. Park, D.S. Yoon, J.H. Park, and T.S. Kim, 'In-situ quantitative analysis of a prostate specific antigen (PSA) using a nanomechanical PZT cantilever,' Lab Chip. Vol. 4, pp. 547-552, 2004   DOI   ScienceOn
20 B. Ilic, D. Czaplewski, H. G. Craighead, 'Mechanical resonant immunospecific biological detector,' Appl. Phys. Lett., Vol. 77, pp. 450-452, 2000   DOI   ScienceOn
21 T. Strick, J.-F. Allemand, V. Croquette, D. Bensimon, 'Twisting and stretching single DNA molecules,' Prog. Biophys. & Mol. Biol., Vol. 74, pp. 115-140, 2000   DOI   ScienceOn
22 D.P. Allison, P. Hinterdorfer, W. Han, 'Biomolecular force measurements and the atomic force microscope,' Curr. Opin. Biotechnol., Vol. 13, pp. 47-51, 2002   DOI   ScienceOn
23 A. W. McFarland, M. A. Poggi, M.J. Doyle, L. A. Bottomley, and J. S. Colton, 'Influence of surface stress on the. resonance behavior of microcantilevers,' Appl. Phys. Lett., Vol. 87, 053505, 2005   DOI   ScienceOn
24 G. Wu, H. Ji, K. Hansen, Y. Thundat, R. Datar, R. Cote, M.F. Hagan, A.K. Chakraborty, and A. Majumdar, 'Origin of nanomechanical cantilever motion generated from biomolecular interactions,' Proc. Natl. Acad. Sci. USA., Vol. 98, pp. 1560-1564, 2001