• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.90-101
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• 2018

Strawberry (Fragaria ${\times}$ ananassa) is a globally-cultivated and popular fruit crop, prized for its flavor and nutritional value. Sweetness, a key determinant of fruit quality, depends on the sugar composition and concentration. We selected eight strawberry cultivars based on the fruit soluble solids content to represent high and low sugar content groups. The average soluble solid content was $13.6^{\circ}Brix$ (Okmae, Geumsil, Aram, and Maehyang) and $2.9^{\circ}Brix$ (Missionary, Camino Real, Portola, and Gilgyung53), for the high and low sugar content groups, respectively. Sucrose was the main sugar in the cultivars with high sugar content, whereas fructose was the main component in the low sugar content cultivars. Fruit starch concentration ranged from $3.247{\pm}0.056$ to $3.850{\pm}0.055g/100g$, with a 12% higher concentration in the high sugar content cultivars. Additionally, we identified 41 sugar metabolism-related genes in Fragaria ${\times}$ ananassa and analyzed the relationship between their transcripts and the sugar accumulation in fruit. FaGPT1, FaTMT1, FaHXK1, FaPHS1, FaINVA-3, and FacxINV2-1 were highly expressed in the high sugar content cultivars, while FapGlcT, FaTMT2-1, FaPHS2-1, FaSUSY1-1, and FaSUSY1-2 were highly expressed in the low sugar content cultivars. In general, a greater number of genes encoding sugar transporters or involved in sugar synthesis were highly expressed in the high sugar content cultivars. Contrarily, genes involved in sugar degradation were preferentially transcribed in the low sugar content cultivars. Although gene expression was not perfectly proportional to sugar content or concentration, our analysis of the genes involved in sugar metabolism and accumulation in strawberries provides a framework for further studies and for the subsequent engineering of sugar metabolism to enhance fruit quality.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.5
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• pp.321-333
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• 2013

As environmental sustainability becomes a key consideration in policy-making, more responsible consumption and utilization in daily life concern both health and quality of life. To address inequities in health in relation to environments, waste management has taken more progressive ways, and one of them is biomass-to-energy conversion that utilizes energy recovery from food waste. By extension, a food waste zero-emission system using fermentation and extinction technology gains much attention, so that this study is designed to examine residents' attitudes toward recycling food waste produced at home and toward food waste zero-emission system. Utilizing a survey questionnaire, this research collected data from 400 individual units of multifamily housing estates nationwide, and the data were analyzed using descriptive and inferential statistics. The findings indicate that food waste generated at home was highly water-contained and produced in the stage of food preparation before cooking while respondents viewed that food waste collection and treatment needed to be improved. It's noted that respondents strongly supported the use of food waste as a energy source and would have the use of the food waste zero-emission system built in kitchen sink. Regression analysis showed that educational attainment of housewives, cooking style, and planning food purchase were statistically significant factors in the attitude of the responded residents toward recycling food waste while none of the factors were in the attitude toward the food waste zero-emission system.

• Journal of the Korean Society for information Management
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• v.33 no.3
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• pp.133-154
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• 2016

Interdisciplinary approach has been recognized as one of key strategies to address various and complex research problems in modern science. The purpose of this study is to investigate the interdisciplinary characteristics and structure of the field of big data. Among the 1,083 journals related to the field of big data, multiple Subject Categories (SC) from the Web of Science were assigned to 420 journals (38.8%) and 239 journals (22.1%) were assigned with the SCs from different fields. These results show that the field of big data indicates the characteristics of interdisciplinarity. In addition, through bibliographic coupling network analysis of top 56 journals, 10 clusters in the network were recognized. Among the 10 clusters, 7 clusters were from computer science field focusing on technical aspects such as storing, processing and analyzing the data. The results of cluster analysis also identified multiple research works of analyzing and utilizing big data in various fields such as science & technology, engineering, communication, law, geography, bio-engineering and etc. Finally, with measuring three types of centrality (betweenness centrality, nearest centrality, triangle betweenness centrality) of journals, computer science journals appeared to have strong impact and subjective relations to other fields in the network.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.246-253
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• 2018

The main industries in Chungcheongbuk-do are the smart IT parts; bio-health; and transportation machinery, materials and parts. The transportation, machinery, materials and parts (TMMP) industry is a key source technology for environmentally friendly and intelligent vehicles related to future vehicles in the machinery sector. Business support projects to improve this industry are currently underway. In this study, the number of enterprises, number of employees, amount of shipment, and added value of the enterprises in Chungbuk area were analyzed for the downstream and rear industries of the transportation, machinery, materials, and parts industry to set targets and directions for the technical support projects during the enterprise support. In particular, for the SMEs with less than 50 employees, the changes in the value added per enterprise were analyzed according to the period from the year of foundation. In the case of the rear industry, the value added by companies within three years after foundation has decreased gradually each year, so technical support is needed at the beginning of the startup. Companies between the ages of five and nine years are showing a fluctuating trend each year. Therefore, it is necessary to support business diversification or find new items.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.72-77
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• 2001

Expression of f3 ~agarase Gene and Catabolite Repression in Escherichia coli by the Promoter of Alginate Lyase Gene Isolated from Marine Pseudomonas sp. Jin, Cheal~Ho, J~Hyeon Park, Jeong-Hyun Han, YoonM Hyeok Chae, Jong~Hee Lee, Jung-Kee Lee!, and In-800 Kong*. Faculty of Food Science and Biotechnology, Pukyong National UniversitYt Pusan 608-737, Korea, llnBioNet Co. 1690-3 Taejon 306-230, Korea - Promoter is a key factor for expression of the recombinant protein. There are many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter ~md association between the promoter mutants, f3 agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids ofthe alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using peR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of f3 -agarase productivity.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.1
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• pp.38-44
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• 2013

To construct the system controlling the aeration volume for D wastewater treatment plant effectively, the fluctuation of aeration volume was analyzed with changes of factors of the influent. As a result, the range of aeration volume was wide to maintain the certain concentration of DO, and the key factor to decide the aeration volume was found to be the temperature, F/M ratio, the loading rate of $BOD_5$ and T-N of the influent. Among the factors, the temperature of the influent had the most decisive effect on the aeration volume. The result showed that $45.8m^3/h$ of the aeration volume was needed with an increase of $1^{\circ}C$ of the influent, and the effect of the season was considered. Since the temperature of the influent is affected by a change of season, same as F/M ratio, the loading rate of the influent and the concentration of MLSS, it seemed that the change of the temperature of the influent affects the aeration volume even more. Therefore, it is preferable to consider the loading rate of the influent and F/M ratio altogether, rather than considering only one factor when deciding aeration volume.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.135-145
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• 2017

The purpose of this study is to present meaningful information and policy implications concerning the determinants of royalties to the government-funded research centers and agencies. These groups are responsible for policies encouraging technology transfer from the public sector to the private sector. To identify key determinants of royalties in technology transfer, this study conducted AHP survey analysis (Survey period: 01/10~31/10, 2016) of 85 companies which were participants of the R&D project "Next Generation BioGreen21" of R.D.A in the "N"center from 2011 to 2015. Research results show that the critical factors include: 1)Technical considerations for determining the profitability of the technologies, 2)The interest and willingness of the management group, 3)Necessity and urgency of technology transfer. These findings suggest three main policy implications. First, the government-funded research centers and agencies should develop technologies that help companies improve their commercialization as well as the profitability in the near future. Second, government-funded research centers and agencies should consider reducing the administrative burden of the royalty payment for private companies. Third, public R&D projects should reflect the proper research schedule for technology development on the basis of the R&D time span of companies which transfer technology.

• KSBB Journal
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• v.29 no.6
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• pp.443-447
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• 2014

Biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) was examined in recombinant Escherichia coli W strain from sucrose. The Pseudomonas sp. MBEL 6-19 phaC1437 gene and the Clostridium propionicum pct540 gene, which encode engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 ($PhaC1_{Ps6-19}$) and engineered C. propionicum propionyl-CoA transferase ($Pct_{Cp}$), respectively, were expressed in E. coli W to construct key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli W expressing the phaC1437 gene and the pct540 gene could synthesize P(3HB-co-13mol%LA) up to the polymer content of 31.3 wt% when it was cultured in chemically defined MR medium containing 20 g/L of sucrose and 2 g/L of sodium 3-hydroxybutyrate. When Ralstonia eutropha phaAB genes were additionally expressed to provide 3-hydroxybutyrate-CoA (3HB-CoA) from sucrose, P(3HB-co-16mol%LA) could be synthesized from sucrose as a sole carbon source without supplement of sodium 3-hydroxybutyrate in culture medium, but the PHA content was decreased to 12.2 wt%. The molecular weight of P(3HB-co-16 mol%LA) synthesized in E. coli W using sucrose as carbon source were $1.53{\times}10^4$ ($M_n$) and $2.78{\times}10^4$ ($M_w$), respectively, which are not different from those that have previously been reported by other recombinant E. coli strains. Engineered E. coli strains developed in this study should be useful for the production of lactate-containing PHAs from sucrose, one of the most abundant and least expensive carbon sources.

• Journal of Life Science
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• v.19 no.9
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• pp.1200-1208
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• 2009

Tight junctions (TJs) that act as paracellular permeability barriers play an essential role in regulating the diffusion of fluid, electrolytes and macromolecules through the paracellular pathway. In this study, we investigated the correlation between the tightening of TJs, permeability and the invasive activity of genistein - a bioactive isoflavone of soybeans - in human breast carcinoma MCF-7 and MDA-MB-231 cells. The inhibitory effects of genistein on cell proliferation, motility and invasiveness were found to be associated with the increased tightness of the TJs, which was demonstrated by an increase in transepithelial electrical resistance and a decrease in paracellular permeability. Additionally, the immunoblotting results indicated that genistein repressed the levels of the proteins that comprise the major components of TJ, claudin-3 and claudin-4, which play a key role in the control and selectivity of paracellular transport. Furthermore, genistein decreased the metastasis-related gene expressions of insulin like growth factor-1 receptor and snail, while concurrently increasing that of thrombospondin-1 and E-cadherin. In addition, we demonstrated that claudins play an important role in the anti-motility and invasiveness of genistein using claudin-3 small interfering RNA. Taken together, our results indicate a possible role for genistein as an inhibitor of cancer cell invasion through the tightening of TJs, which may counteract the up-regulation of claudins. In addition, our results indicate that this may be beneficial for the inhibition of tumor metastasis.

• Molecules and Cells
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• v.38 no.7
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• pp.643-650
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• 2015

The use of conditioned medium from mesenchymal stem cells may be a feasible approach for regeneration of bone defects through secretion of various components of mesenchymal stem cells such as cytokines, chemokines, and growth factors. Mesenchymal stem cells secrete and accumulate multiple factors in conditioned medium under specific physiological conditions. In this study, we investigated whether the conditioned medium collected under hypoxic condition could effectively influence bone regeneration through enhanced migration and adhesion of endogenous mesenchymal stem cells. Cell migration and adhesion abilities were increased through overexpression of intercellular adhesion molecule-1 in hypoxic conditioned medium treated group. Intercellular adhesion molecule-1 was upregulated by microRNA-221 in mesenchymal stem cells because microRNAs are key regulators of various biological functions via gene expression. To investigate the effects in vivo, evaluation of bone regeneration by computed tomography and histological assays revealed that osteogenesis was enhanced in the hypoxic conditioned medium group relative to the other groups. These results suggest that behavioral changes of endogenous mesenchymal stem cells through microRNA-221 targeted-intercellular adhesion molecule-1 expression under hypoxic conditions may be a potential treatment for patients with bone defects.