• 제목/요약/키워드: Bio-film

검색결과 448건 처리시간 0.019초

Liquid Crystal-based Imaging of Biomolecular Interactions at Roller Printed Protein Surfaces

  • Park, Min-Kyung;Jang, Chang-Hyun
    • Bulletin of the Korean Chemical Society
    • /
    • 제31권5호
    • /
    • pp.1223-1227
    • /
    • 2010
  • In this study, the orientational behavior of thermotropic liquid crystals (LC) supported on a film of protein receptors was examined. Avidin was roller printed and covalently immobilized onto the surface of gold using NHS/EDC chemistry. The orientation of nematic 4-cyano-4'-pentylbiphenyl (5CB) was found to be parallel to the plane of the printed avidin surface before incubation with a solution of biotin. However, protein-receptor complexation induced a random orientation of 5CB, where protein-receptor complexes disturbed the nanoscale topography of the printed protein surface. Atomic force microscopy and ellipsometry was used to confirm printing and the specific interaction of proteins. These results demonstrate that the combination of LC and roller printing can be used to detect specific interactions between biomolecules by manipulating the orientational behavior of LC to the printed protein surfaces.

Effective Parameters for the Precise Control of Thin Film Buckling on Elastomeric Substrates

  • Ahn, Seong-Min;Jang, Chang-Hyun
    • Bulletin of the Korean Chemical Society
    • /
    • 제31권2호
    • /
    • pp.419-422
    • /
    • 2010
  • This paper reports a simple and versatile technique for generating highly controllable sinusoidal nanostructures on the surface of poly-(dimethylsiloxane) (PDMS). The sinusoidal features were generated by oxidizing PDMS slabs with oxygen plasma, then stretching them by wrapping around a cylinderical surface, and finally allowing them to relax. The wavelength and amplitude could be finely controlled by varying the fabrication conditions such as duration of oxidation, diameter of the glass cylinder, duration of stretching, thickness of the PDMS slabs, and temperature during the second hardening process. The varied trends of the buckling patterns were characterized by using an atomic force microscope.

계면활성제처리 무가온 온실내 겨울철과 봄철의 상추 생장 비교 (Comparison of Lettuce Growth between Winter and Spring in Non-Heated Greenhouse Covered with Sufactants Film)

  • 전희;이혜은;김학주;이시영;남윤일;박태욱;도현성
    • 한국생물환경조절학회:학술대회논문집
    • /
    • 한국생물환경조절학회 2003년도 춘계 학술대회 논문집
    • /
    • pp.34-37
    • /
    • 2003
  • 1980년대 이후 비닐하우스를 이용하여 수박, 참외, 딸기, 오이, 토마토, 풋고추, 상추, 배추, 시금치와 같은 채소류와 장미, 국화, 카네이션, 나리와 같은 화훼류 등에서 농업의 생산성을 크게 향상시켰다. 특히 2001년도를 기준으로 33조원에 달하는 농업총생산액 가운데 이들 시설채소류의 생산액은 9% 정도인 3조원에 이른다. 시설원예라고 불리는 이 농업분야는 추운 겨울에도 신선한 채소를 공급함으로서 식생활 개선에 크게 이바지하고, 다양한 꽃을 연중 보급하여 삶의 질을 향상시켰다. (중략)

  • PDF

계면활성제 농도가 폴리에틸렌필름온실의 수적성에 미치는 영향 (Effect of Surfactants Concentration on Waterdrop in Polyethylene Film Greenhouse)

  • 전희;이혜은;김학주;이시영;남윤일;박태욱;도현성
    • 한국생물환경조절학회:학술대회논문집
    • /
    • 한국생물환경조절학회 2003년도 춘계 학술대회 논문집
    • /
    • pp.92-97
    • /
    • 2003
  • 비닐하우스에서 피복자재로 폴리에틸렌(polyethylene), 초산비닐(ethylene vinyl-acetate) 및 염화비닐(polyvinylchloride)와 같은 연질필름을 90% 이상 사용하고 있으나 필름 표면에 물방울이 맺히는 것을 방지하는 무적성과 각종 먼지 등이 부착하는 것을 억제하는 방진성의 저하로 시설내 투광률이 5-l5% 떨어지고 다습하여 딸기와 같은 작물에 해로운 잿빛곰팡이병 발생이 심하여 생산성이 크게 떨어지고 있다('92, '97 원예연구소, '91 Jaffrin). (중략)

  • PDF

계면활성제 농도가 폴리에틸렌필름온실의 미기상에 미치는 영향 (Effect of Surfactants Concentration on Micro-environment in Polyethylene Film Greenhouse)

  • 전희;이혜은;김학주;이시영;남윤일;박태욱;도현성
    • 한국생물환경조절학회:학술대회논문집
    • /
    • 한국생물환경조절학회 2003년도 춘계 학술대회 논문집
    • /
    • pp.86-91
    • /
    • 2003
  • 비닐하우스 내외의 온도 차이로 발생하는 공기 중 수분의 결로현상 때문에 필름 표면에 물방울이 맺히게 된다. 특히 내부에서는 식물의 꽃이 피고 열매를 맺기 위하여 상대습도 60% 이상이 유지되는 것이 좋기 때문에 사람이 거주하는 건물 내부보다 훨씬 다습한 조건이 주어지기에 비닐하우스 내부에서는 물방울 발생의 소지가 높다. (중략)

  • PDF

Fragment Molecular Orbital Method: Application to Protein-Ligand Binding

  • Watanabe, Hirofumi;Tanaka, Shigenori
    • Interdisciplinary Bio Central
    • /
    • 제2권2호
    • /
    • pp.6.1-6.5
    • /
    • 2010
  • Fragment molecular orbital (FMO) method provides a novel tool for ab initio calculations of large biomolecules. This method overcomes the size limitation difficulties in conventional molecular orbital methods and has several advantages compared to classical force field approaches. While there are many features in this method, we here focus on explaining the issues related to protein-ligand binding: FMO method provides useful interaction-analysis tools such as IFIE, CAFI and FILM. FMO calculations can provide not only binding energies, which are well correlated with experimental binding affinity, but also QSAR descriptors. In addition, FMO-derived charges improve the descriptions of electrostatic properties and the correlations between docking scores and experimental binding affinities. These calculations can be performed by the ABINIT-MPX program and the calculation results can be visualized by its proper BioStation Viewer. The acceleration of FMO calculations on various computer facilities is ongoing, and we are also developing methods to deal with cytochrome P450, which belongs to the family of drug metabolic enzymes.