• Title/Summary/Keyword: Bio-column

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Structural Properties of Steel-glulam Composite Column

  • Jang, Sang-Sik;Kim, Yun-Hui;Shin, Il-Joong
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.5
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    • pp.399-404
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    • 2010
  • A new green home designed to save money while at the same time saving the environment with some of the finest green features available in the market. Composite column composed of structural steel and structural glued laminated timber is avery Eco-friendly building products for design building because that use recycled or second hand. For compare to compressive strength of structural glued laminated timber (glulam), structural steel, and composite column (steel-glulam), tested compressive strength of each specimen. 1) structural glued laminated timber : Theoretical compressive strength is 151.6 kN similar to elastic limits. 2) structural steel (H type) : Theoretical compressive strength is 148.2 kN little under the elastic limits. 3) structural steel (D type) : Theoretical compressive strength is 147.3 kN upper than the elastic limits. 4) composite column : Actual elastic limits are about 600 kN. Result in, composite column improve compressive strength of Structural steel column and provide structural stability of the building.

Direct Bio-regeneration of Nitrate-laden Ion-exchange Resin (질산성질소에 파과된 이온교환수지의 생물학적 직접 재생)

  • Nam, Youn-Woo;Bae, Byung-Uk
    • Journal of Korean Society on Water Environment
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    • v.29 no.6
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    • pp.777-781
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    • 2013
  • Ion-exchange technology is one of the best for removing nitrate from drinking water. However, problems related to the disposal of spent brine from regeneration of exhausted resins must be overcome so that ion exchange can be applied more widely and economically, especially in small communities. In this background, a combined bio-regeneration and ion-exchange system was operated in order to prove that nitrate-laden resins could be bio-regenerated through direct contact with denitrifying bacteria. A nitrate-selective A520E resin was successfully regenerated by denitrifying bacteria. The bio-regeneration efficiency of nitrate-laden resins increased with the amount of flow passed through the ion-exchange column. When the fully exhausted resin was bio-regenerated for 5 days at the flowrate of 30 BV/hr and MLSS concentration of $125{\pm}25mg/L$, 97.5% of ion-exchange capacity was recovered. Measurement of nitrate concentrations in the column effluents also revealed that less than 5% of nitrate was eluted from the resin during 5 days of bio-regeneration. This result indicates that the main mechanism of bio-regeneration is the direct reduction of nitrate by denitrifying bacteria on the resin.

Synthesis of molecularly imprinted polymer (MIP) by radiation-induced polymerization and separation of ferulic acid from rice oil using MIP-packed column

  • Yoon, Seok-Kee;Lee, Jae-Chan;Lee, Seung-Ho;Choi, Seong-Ho;Kim, Hwa-Jung;Park, Hae-Jun;Kang, Hee-Dong
    • Analytical Science and Technology
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    • v.19 no.3
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    • pp.218-225
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    • 2006
  • A molecularly imprinted polymer (MIP) was synthesized by radiation-induced polymerization (RIP), where the ferulic acid was used as a template molecule, 4-vinylpyridine as a monomer and ethylene glycoldimethacrylate (EGDMA) as a cross-linking monomer. The MIP was packed in a glass column using a slurry method for use in medium pressure liquid chromatography (MPLC). The MPLC column was tested for separation and purification of ferulic acid from the rice oil. When repeated three times, the MPLC separation/purification yielded the ferulic acid with the purity higher than ~99%. The chemiluminescence of the luminal (5-amino-2,3-dihydro-1,4-phtalazinedione) measured on a potato disc slide (5.0 mm thick) was enhanced in the presence of ferulic acid, while, without the ferulic acid, the chemiluminescence of luminol on the potato slice disc was not observed, which suggests the ferulic acid obtained from the rice oil can be useful for immunoassay.

A comparison study of extraction methods for bio-liquid via hydrothermal carbonization of food waste

  • Bang, YeJin;Choi, Minseon;Bae, Sunyoung
    • Analytical Science and Technology
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    • v.31 no.3
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    • pp.112-121
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    • 2018
  • The hydrothermal carbonization method has received great attention because of the conversion process from biomass. The reaction produces various products in hydrochar, bio-liquid, and gas. Even though its yield cannot be ignored in amount, it is difficult to find research papers on bio-liquid generated from the hydrothermal carbonization reaction of biomass. In particular, the heterogeneity of feedstock composition may make the characterization of bio-liquid different and difficult. In this study, bio-liquid from the hydrothermal carbonization reaction of food wastes at $230^{\circ}C$ for 4 h was investigated. Among various products, fatty acid methyl esters were analyzed using two different extraction methods: liquid-liquid extraction and column chromatography. Different elutions with various solvents enabled us to categorize the various components. The eluents and fractions obtained from two different extraction methods were analyzed by gas chromatography with a mass spectrometer (GC/MS). The composition of the bio-liquid in each fraction was characterized, and seven fatty acid methyl esters were identified using the library installed in GC/MS device.

Bio-barrier Formation by Biomass Injection into Soil (미생물 토양 주입을 통한 Bio-barrier 형성)

  • Kim, Geon-Ha;Song, Youngwoo;Gu, Dongyoung
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.5
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    • pp.927-938
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    • 2000
  • When microorganism is injected into porous medium such as soils along with appropriate substrate and nutrients, biomass retained in the soil pore. Soil pore size and shape are varied from the initial condition as a result of biofilm formation, which make hydraulic conductivity reduced. In this research, hydraulic conductivity reduction was measured after microorganism are inoculated and cultured with synthetic substrates and nutrients. Biomass-soil mixture was evaluated its applicability to the field condition as an alternative liner material in landfill by measuring hydraulic conductivity change after repetitive freeze-thaw cycles. Resistance of biofilm to chemical solution and degree of biodegradation were measured through column test.

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Rapid Separation of Cellular Cyclosophoraoses Produced by Rhizobium Species

  • Seo, Dong-Hyuk;Lee, Sang-Hoo;Park, Hey-Lin;Kwon, Tae-Jong;Jung, Seun-Ho
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.522-525
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    • 2002
  • A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

Bio-regeneration of Ion-exchange Resin for Treating Reverse Osmosis Concentrate (RO 농축폐액의 처리를 위한 이온교환수지의 생물재생)

  • Bae, Byung-Uk;Nam, Youn-Woo
    • Journal of Korean Society on Water Environment
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    • v.30 no.5
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    • pp.517-523
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    • 2014
  • In order to remove both nitrate and sulfate present in the concentrate of RO(reverse osmosis) process, a combined bio-regeneration and ion-exchange(IX) system was studied. For this purpose, both denitrifying bacteria(DNB) and sulfate reducing bacteria(SRB) were simultaneously cultivated in a bio-reactor under anaerobic conditions. When the IX column containing a nitrate-selective A520E resin was fully exhausted by nitrate and sulfate, the IX column was bio-regenerated by pumping the supernatant of the bio-reactor, which contains MLSS concentration of $125{\pm}25mg/L$, at the flowrate of 360 BV/hr. Even though the nitrate-selective A520E resin was used, the breakthrough curves of ionic species showed that sulfate was exhausted earlier than nitrate. The reason for this result is due to the fact that the concentration of sulfate in RO concentrate was 36 to 48 times higher than nitrate. The bio-reactor was successfully operated at a volumetric loading rate of 0.6 g $COD/l{\cdot}d$, nitrate-N loading rate of 0.13 g $NO_3{^-}-N/l{\cdot}d$, and sulfate loading rate of 0.08 g $SO_4{^{2-}}/l{\cdot}d$. The removal rate of SCOD, nitrate-N, sulfate was 90, 100, and 85%, respectively. When the virgin resin was fully exhausted and consecutively bio-regenerated for 2 days, 81% of nitrate and 93% of sulfate were reduced. When the virgin resin was repeatedly used up to 4 cycles of service and bio-regeneration, the ion-exchange capacity of bio-regenerated resin decreased to 95, 91, 88, and 81% of virgin resin.

Infiltration Rates of Liquid Pig Manure with Various Dilution Ratios in Three Different Soil (돈분액비 토양침투율과 토양내 분포 특성)

  • Shim, Ho-Young;Lee, Kyo-Suk;Lee, Dong-Sung;Jeon, Dae-Sung;Park, Mi-Suk;Shin, Ji-Su;Lee, Yun-Koung;Goo, Ji-Won;Kim, Soo-Bin;Song, Seong-Geun;Chung, Doug-Young
    • Korean Journal of Environmental Agriculture
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    • v.33 no.3
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    • pp.164-168
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    • 2014
  • BACKGROUND: Increase in application of liquid pig manure(LPM) in agriculture as nutritional source has become a social issue due to its influence on water quality. Also, proper application methods have not been developed with respect to indigenous properties of LPM and soil physical properties. Therefore, we conducted this experiment to observe the infiltration characteristics and distribution of dissolved organic compounds of LPM in soils having different soil textures. METHODS AND RESULTS: To do this experiment, we collected three different soils and LPM. We analyzed the physical and chemical properties of both soils and LPM to determine the dilution ratios of LPM. The LPM diluted to 4 different ratios with distilled water was applied to the top of soil column. Infiltration rates were observed by time and depth until the amount of effluent collected from the bottom of the soil columns were stabilized while maintaining the hydraulic head 3 cm above the soil column. The results showed that infiltration rates increased with increasing dilution ratios in the order of sandy, loamy sand, and sandy loam. The time required to reach steady state was increased with decreasing sand contents clay. CONCLUSION: The size and amount of the dissolved organic compounds in LPM that can determine the efficiency as fertilizer and environmental problems as nonpoint pollution source in water quality have not been investigated with respect to behavior and transport of them in soil. Therefore, it requires further research how we can properly apply LPM as valuable fertilizer substitute for inorganic fertilizers.

Bio Sparging Column Experiment for Remediation of Diesel Contaminated Groundwater (디젤오염 지하수 정화를 위한 공기주입정화법 칼럼 실험)

  • Chang Soon-Woong;Lee Si-Jin;Song Jung-Hoon;Kwon Soo-Youl
    • Journal of Environmental Science International
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    • v.13 no.12
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    • pp.1059-1065
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    • 2004
  • Bio sparging experiments were conducted in a laboratory column to investigate the potential removal of diesel contaminated groundwater. The objectives in this study were (a) to determine the extent of diesel degradation in laboratory columns under supplement of nutrient; (b) to determine the effect of variation of air flow in the removal of diesel and (c) to evaluate the potential enhancement of diesel degradation as a function of temperature. Our results showed that the nutrient supplement and higher air flow greatly enhanced diesel degradation. However, the variation of water temperature examined slightly increased degradation rate of diesel fuel.

Removal of Benzene in Solution by using the Bio-carrier with Dead Bacillus drentensis sp. and Polysulfone (Bacillus drentensis sp. 사균과 polysulfone으로 이루어진 미생물담체를 이용한 수용액 내 벤젠 제거)

  • Park, Sanghee;Lee, Minhee
    • Journal of Soil and Groundwater Environment
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    • v.18 no.1
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    • pp.46-56
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    • 2013
  • Laboratory scale experiments to remove benzene in solution by using the bio-carrier composed of dead biomass have been performed. The immobilized bio-carrier with dead Bacillus drentensis sp. and polysulfone was manufactured as the biosorbent. Batch sorption experiments were performed with bio-carriers having various quantities of biomass and then, their removal efficiencies and uptake capacities were calculated. From results of batch experiments, 98.0% of the initial benzene (1 mg/L) in 1 liter of solution was removed by using 40 g of immobilized bio-carrier containing 5% biomass within 1 hour and the biosorption reaction reached in equilibrium within 2 hours. Benzene removal efficiency slightly increased (99.0 to $99.4%{\pm}0.05$) as the temperature increased from 15 to $35^{\circ}C$, suggesting that the temperature rarely affects on the removal efficiency of the bio-carrier. The removal efficiency changed under the different initial benzene concentration in solution and benzene removal efficiency of the bio-carrier increased with the increase of the initial benzene concentration (0.001 to 10 mg/L). More than 99.0% of benzene was removed from solution when the initial benzene concentration ranged from 1 to 10 mg/L. From results of fitting process for batch experimental data to Langmuir and Freundlich isotherms, the removal isotherms of benzene were more well fitted to Freundlich model ($r^2$=0.9242) rather than Langmuir model ($r^2$=0.7453). From the column experiment, the benzene removal efficiency maintained over 99.0% until 420 pore volumes of benzene solution (initial benzene concentration: 1 mg/L) were injected in the column packed with bio-carriers, investigating that the immobilized carrier containing Bacillus drentensis sp. and polysulfone is the outstanding biosorbent to remove benzene in solution.