• Title/Summary/Keyword: Bio-analysis

Search Result 3,811, Processing Time 0.033 seconds

Histological Comparative Study of Rabbit Maxillary Sinus Augmentation with Bio-Oss and β-TCP (Bio-Oss와 β-TCP를 이용한 토끼 상악동 거상술 후의 조직학적 비교 연구)

  • Moon, Yong-Suk
    • Journal of Life Science
    • /
    • v.28 no.10
    • /
    • pp.1220-1232
    • /
    • 2018
  • The purpose of this animal study was to evaluate, by histological analysis, bone regeneration in rabbit maxillary sinuses with an anorganic bovine graft (Bio-Oss) and a ${\beta}-tricalcium$ phosphate (${\beta}-TCP$) grafting. Bilateral sinus augmentation procedures were performed in 12 adult male rabbits. Rectangular replaceable bony windows were made with a piezoelectric thin saw insert. In the Bio-Oss group, Bio-Oss was grafted and in the ${\beta}-TCP$ group, ${\beta}-TCP$ was grafted and covered by replaceable bony windows. The animals were sacrificed at 2, 4, and 8 weeks after the surgical procedure. The augmented sinuses were evaluated by histomorphometric analysis using hematoxylin-eosin, Masson trichrome, and tartrate-resistant acid phosphatase stains and also by immunohistochemical analysis of proliferating cell nuclear antigen (PCNA), type I collagen, and osteocalcin content. Histologically, new bone formation was found on the surface of Bio-Oss and ${\beta}-TCP$ particles from 2 weeks and continued to 8 weeks. Significant higher new bone formation was revealed in the ${\beta}-TCP$ group than in the Bio-Oss group at 8 weeks. The amount of graft materials was significantly decreased in the ${\beta}-TCP$ group and the number of osteoclasts was significantly increased in the ${\beta}-TCP$ group from 4 to 8 weeks. Immunoreactivity to PCNA was reduced at 8 weeks. The expression of type I collagen was significantly increased in the ${\beta}-TCP$ group at 2 weeks, but was significantly increased in the Bio-Oss group at 8 weeks. Immunoreactivity to osteocalcin was increased from 2 to 8 weeks. These histological results can help in the selection of graft materials for implants. Both Bio-Oss and ${\beta}-TCP$ are proven graft materials, however, these results indicate that ${\beta}-TCP$ showed better bone regeneration results in rabbit maxillary sinus augmentation.

A study on application of eco-friendly follow-up process connected with livestock wastewater treatment plant using the upflow constructed wetland (가축분뇨처리시설과 연계한 상향류식 인공습지의 자연형 후속처리공정 적용방안에 관한 연구)

  • Choi, Hanna;Cho, Eunha;Kang, Hogeun;Park, Joohyun;Kang, Seonhong
    • Journal of Korean Society of Water and Wastewater
    • /
    • v.29 no.3
    • /
    • pp.359-370
    • /
    • 2015
  • This study developed an up-flow wetland providing either an eco-friendly follow-up process of medium-sized public treatment facility for livestock manure or a non-point source pollution controller near livestock farms. The four bench-scale up-flow wetlands were operated with four different bed media sets. The removal efficiencies of the wetland effluent for CODCr, TN, TP, SS were 35.2 %, 29.5 %, 31.2 % and 52.2 % for set 1(Blank, without reed, with bio-ceramic), 40.6 %, 43.4 %, 42.2 % and 55.4 % for set 2(with bio-reed & without bio-ceramic), 45.2 %, 48.7 %, 46.6 % and 66.3 % for set 3(with bio-reed & bio-ceramic), 32.9 %, 27.3 %, 29.3 % and 54.1 % for Set 4(with reed & bio ceramic), respectively. The set 3 condition having a mixture of bio-reed and bio-ceramic showed the highest efficiency in the bench-scale evaluation. This study suggests a mixture of bio-reed and bio-ceramic as suitable bed media in the construction of artificial wetlands near livestock farms. Soils including the bed media were monitored during the evaluation for trace elements. Soil analysis results were satisfied with the Korean Soil Contamination Standard. This study showed that the up-flow constructed wetland was feasible to treat the effluent livestock wastewater treatment facility.

Chemical Imaging Analysis of the Micropatterns of Proteins and Cells Using Cluster Ion Beam-based Time-of-Flight Secondary Ion Mass Spectrometry and Principal Component Analysis

  • Shon, Hyun Kyong;Son, Jin Gyeong;Lee, Kyung-Bok;Kim, Jinmo;Kim, Myung Soo;Choi, Insung S.;Lee, Tae Geol
    • Bulletin of the Korean Chemical Society
    • /
    • v.34 no.3
    • /
    • pp.815-819
    • /
    • 2013
  • Micropatterns of streptavidin and human epidermal carcinoma A431 cells were successfully imaged, as received and without any labeling, using cluster $Au_3{^+}$ ion beam-based time-of-flight secondary ion mass spectrometry (TOF-SIMS) together with a principal component analysis (PCA). Three different analysis ion beams ($Ga^+$, $Au^+$ and $Au_3{^+}$) were compared to obtain label-free TOF-SIMS chemical images of micropatterns of streptavidin, which were subsequently used for generating cell patterns. The image of the total positive ions obtained by the $Au_3{^+}$ primary ion beam corresponded to the actual image of micropatterns of streptavidin, whereas the total positive-ion images by $Ga^+$ or $Au^+$ primary ion beams did not. A PCA of the TOF-SIMS spectra was initially performed to identify characteristic secondary ions of streptavidin. Chemical images of each characteristic ion were reconstructed from the raw data and used in the second PCA run, which resulted in a contrasted - and corrected - image of the micropatterns of streptavidin by the $Ga^+$ and $Au^+$ ion beams. The findings herein suggest that using cluster-ion analysis beams and multivariate data analysis for TOF-SIMS chemical imaging would be an effectual method for producing label-free chemical images of micropatterns of biomolecules, including proteins and cells.

New Methodology to Develop Multi-parametric Measure of Heart Rate Variability Diagnosing Cardiovascular Disease

  • Jin, Seung-Hyun;Kim, Wuon-Shik;Park, Yong-Ki
    • International Journal of Vascular Biomedical Engineering
    • /
    • v.3 no.2
    • /
    • pp.17-24
    • /
    • 2005
  • The main purpose of our study is to propose a new methodology to develop the multi-parametric measure including linear and nonlinear measures of heart rate variability diagnosing cardiovascular disease. We recorded electrocardiogram for three recumbent postures; the supine, left lateral, and right lateral postures. Twenty control subjects (age: $56.70{\pm}9.23$ years), 51 patients with angina pectoris (age: $59.98{\pm}8.41$ years) and 13 patients with acute coronary syndrome (age: $59.08{\pm}9.86$ years) participated in this study. To develop the multi-parametric measure of HRV, we used the multiple discriminant analysis method among statistical techniques. As a result, the multiple discriminant analysis gave 75.0% of goodness of fit. When the linear and nonlinear measures of HRV are individually used as a clinical tool to diagnose cardiac autonomic function, there is quite a possibility that the wrong results will be obtained due to each measure has different characteristics. Although our study is a preliminary one, we suggest that the multi-parametric measure, which takes into consideration the whole possible linear and nonlinear measures of HRV, may be helpful to diagnose the cardiovascular disease as a diagnostic supplementary tool.

  • PDF

Unification System for Analysis of DNA Sequence (DNA 서열 분석을 위한 통합 시스템)

  • Song, Young-Ohk;Chang, Duk-Jin
    • The Journal of the Korea Contents Association
    • /
    • v.11 no.3
    • /
    • pp.65-72
    • /
    • 2011
  • We stand at real world that some practical use method of gene information appears in succession by entrance on the stage of advanced techonlogy. As a lot of studies and development are achieved based on analysis of bio data, necessity of a tool that can help correct interpretation of data is required more and more in a lot of targets of bioinformatics to search new relation and information are established. In this paper, we are offered in existing I wish to offer user a more convenient study tool developing system that can supplement shortcomings of various tools for data analysis. So we've designed to offer in united environment that is not environment that is parted ORF driving out, bio information retrieval and work of similarity comparison lamp to work for bio data analysis and offers lacking consecutiveness in existing analysis system.

Analysis of Pesticides in Herbal Medicine by QuEChERS and GC-MS/MS (QuEChERS 전처리와 GC-MS/MS를 활용한 생약의 잔류농약 분석 가능성 연구)

  • Seo, Go Eun;Kim, A Young;Pyo, Byoung Sik;Lee, Kyoung in
    • Korean Journal of Pharmacognosy
    • /
    • v.51 no.3
    • /
    • pp.207-216
    • /
    • 2020
  • The analysis method for qualitative analysis of the screening method that can be performed prior to the quantitative analysis of individual pesticide was reviewed in order to meet the safety standards that are being strengthened in the field of pesticide residue testing of herbal medicines. Among the residual pesticides presented in the Korean Pharmacopoeia, 56 pesticides, excluding 15 pesticides that need to be individually analyzed, were selected for analysis using QuEChERS preprocessing and GC-MS/MS, which are used in the existing agricultural products field. For each pesticide, the detection limit level of 0.001-0.005 mg/kg and the quantitative limit level of 0.002-0.017 mg/kg were confirmed. In the recovery test in which the standard was treated at a concentration of 0.02 mg/kg, it was confirmed that the proportion of pesticides satisfying the recovery of 70-120% was 85.7-96.4% for each herbal medicine, so it was confirmed that it was a level that could be reviewed by the screening method.