• 한국IT서비스학회지
• /
• 제11권sup호
• /
• pp.89-101
• /
• 2012

This paper presents development of a management system for the health and diseases of the elderly. This study aimed to the promotion of the u-healthcare industry and to the increase in its competitive power by developing and expanding a system for managing the health and diseases of the elderly, in cooperation with u-healthcare companies. The study is underway through the following four substudies. In the first substudy titled development of the bio-signal collection and analysis technology using smart media, a technology that supports the collection of bio-signals in the elderly using portable terminals. In the second substudy titled development of the patient-specific healthcare platform expansion and enhancement technology, a technology is being developed for making medical decisions and taking measures based on the results of the processing of the collected bio-signals. In the third substudy titled development of the N-screen based healthcare contents open service technology, a technology is being developed to provide information on health, diseases, and medicine to platforms. In the fourth substudy titled development of the oriental medicine diagnosis and analysis technology for senile diseases. This study is expected to help ensure an excellent workforce and new technologies in the healthcare sector using smart phones, and to help reduce medical expenses by improving the health of citizens.

• Journal of Periodontal and Implant Science
• /
• 제30권2호
• /
• pp.483-490
• /
• 2000

The aim of this study was to analyze the reliability of an ultrasonic device(SDM) measuring soft tissue thickness in relation to tooth position, and to find factors which can influence the reliability. The results showed that 1. measurement error was the largest in the maxillary second molar position and the smallest in the mandibular central incisor position. 2. in a box whisker plot, the difference between two measurements was most widely distributed in molar positions. 3. in Pearson correlation analysis, the relationship between two measurements was the highest in the maxillary lateral incisor position and, the lowest in the maxillary second molar, mandibular first and second premolar position. 4. a stepwise multiple regression analysis could explain the difference of two measurements with various independent variables in 29.7% (P<0.0001). Gingival thickness was the only variable influencing the measurement difference in a statistically significant level(P<0.0001). It can be concluded that its high reliability, ease to use and patient comfort justified the application of the SDM in measurement of soft tissue thickness.

• Journal of Microbiology and Biotechnology
• /
• 제12권5호
• /
• pp.801-806
• /
• 2002

A multiplex competitive polymerase chain reaction (PCR) method was developed for the rapid identification and quantification of Leuconostoc mesnteroides and Lactobacillus plantarum populations which are the key microorganisms in kimchi fermentation. The strain-specific primers were designed to selectively amplify the target genes encoding 165 rRNA of L. plantarum and dextransucrase of L. mesenteroides. There was a linear relationship between the band intensity of PCR products and the number of colony forming units of each model organism. The PCR quantification method was compared with a traditional plate-counting method f3r the enumeration of the two lactic acid bacteria in a mixed suspension culture and also applied to a real food system, namely, watery kimchi. The population dynamics of the two model organisms in the mixed culture were reliably predictable by the competitive PCR analysis.

• Natural Product Sciences
• /
• 제26권1호
• /
• pp.97-107
• /
• 2020

Isolation of oils from leaves of Juniperus phoenicea and Juniperus oxycedrus was obtained by steam distillation extraction method. The compositions of essential oils (EOs) were studied by means of GC-MS and GC-FID, using the internal standard method and relative response factors. Around ninety eight compounds were determined in total, representing 98.25 g/100 g of EO of J. phoenicea and 98.48 g/100 g of EO of J. oxycedrus, respectively. The volatile leaf oils were dominated by the terpenic hydrocarbon fractions (79.87 g/100 g) and (61.27 g/100 g) characterized by high contents of α-pinene (64.6 g/100 g) and (54.0 g/100 g) in J. phoenicea and J. oxycedrus, respectively, as the main component. Also, the enantiomeric distribution of α-pinene, sabinene, camphene, δ-3-carene, β-pinene, limonene, linalool, terpinen-4-ol, bornyl acetate, and borneol in both oils is presented for the first time.

• 한국버섯학회지
• /
• 제11권4호
• /
• pp.181-186
• /
• 2013

${\gamma}$-Aminobutyric acid(GABA) is a four carbon non-protein amino acid that has several well-known physiological functions, such as a postsynaptic inhibitory neurotransmitter in the brain and induction of hypotensive and tranquilizer effects. A lactic acid bacterium was isolated from button mushroom bed, which is showing high GABA productivity by TLC or HPLC analysis. The strain was identified as Lactobacillus hilgardii by analysis of 16S rDNA gene sequence. When the maximum production of GABA by L. hilgardii was investigated with various concentration of monosodium glutamate, the yield of GABA reached to be 53.65 mM at 1% mono sodium glutamate (MSG) in flask cultivation. A Glutamate decarboxylase (GAD) enzyme, which was known to convert MSG to GABA, was purified from a cell-free extract of L. hilgardii and the molecular weights of purified GAD was estimated to 60,000 by SDS-PAGE. The optimum pH and temperature of GAD were at pH4.6 and at $37^{\circ}C$, respectively. The GAD activity was increased by the addition of sulfate ions such as ammonium sulfate, sodium sulfate and magnesium sulfate, indicating that the increase of hydrophobic interaction causes the increase of GAD activity.

• Journal of Biosystems Engineering
• /
• 제28권6호
• /
• pp.537-544
• /
• 2003

The objectives of this study were to investigate the failure characteristics of a total of 90 parts of tractor driveline, and to predict their average annual demands required to perform the after-sales service. The failure characteristics such as failure mode, mean time between failures, characteristic life and reliability were analyzed using the data collected through the experienced mechanics at the part centers of the tractor manufacturers. The analysis was based on the assumption that the failure distribution follows the Weibull distribution. The average annual demands were also predicted for the replacement parts using the mean time between failures and the renewal theory based on the Weibull distribution. The results of the study revealed that the driveline parts failure was mostly from wearout and their average characteristic life is about 1.760 hours. The estimated mean time between failures was in a range of 670∼3,740 hours and reliability in a range of 40∼60％. The annual replacement demands were in a range of 4∼45 for a service of 100 tractors.

• Bulletin of the Korean Chemical Society
• /
• 제29권3호
• /
• pp.656-662
• /
• 2008

Antagonists of the d -opioid receptor are effective in overcoming resistance against analgesic drugs such as morphine. To identify novel antagonists of the d -opioid receptor that display high potency and low resistance, we performed 3D-QSAR analysis using chemical feature-based pharmacophore models. Chemical features for d -opioid receptor antagonists were generated using quantitative (Catalyst/HypoGen) and qualitative (Catalyst/HipHop) approaches. For HypoGen analysis, we collected 16 peptide and 16 non-peptide antagonists as the training set. The best-fit pharmacophore hypotheses of the two antagonist models comprised identical features, including a hydrophobic aromatic (HAR), a hydrophobic (HY), and a positive ionizable (PI) function. The training set of the HipHop model was constructed with three launched opioid drugs. The best hypothesis from HipHop included four features: an HAR, an HY, a hydrogen bond donor (HBD), and a PI function. Based on these results, we confirm that HY, HAR and PI features are essential for effective antagonism of the d -opioid receptor, and determine the appropriate pharmacophore to design such antagonists.

• Bulletin of the Korean Chemical Society
• /
• 제30권8호
• /
• pp.1724-1728
• /
• 2009

Severe acute respiratory syndrome coronavirus (SARS-CoV) helicase separates the double-stranded nucleic acids using the energy from ATP hydrolysis. We have measured ATPase activity of SARS-CoV helicase in the presence of various types of nucleic acids. Steady state ATPase analysis showed that poly(U) has two-times higher turnover number than poly(C) with lower Michaelis constant. When M13 single-stranded DNA is used as substrate, the Michaelis constant was about twenty-times lower than poly(U), whereas turnover numbers were similar. However, stimulation of ATPase activity was not observed in the presence of double-stranded DNA. pH dependent profiles of ATP hydrolysis with the helicase showed that the optimal ATPase activities were in a range of pH 6.2 ~ 6.6. In addition, ATP hydrolysis activity assays performed in the presence of various divalent cations exhibited that $Mg^{2+}$ stimulated the ATPase activity with the highest rate and $Mn^{2+}$ with about 40% rate as compared to the $Mg^{2+}$.

• Journal of Periodontal and Implant Science
• /
• 제38권1호
• /
• pp.83-90
• /
• 2008

Purpose: This study investigated the osteoconductivity of natural calcium carbonate-derived bone substitutes, hen eggshell (ES), and compared with those of commercial bone substitutes. Materials and Methods: Osseous defects created in the rat calvaria were filled with particulated ES(ES-1), ES with calcium-deficient hydroxyapatite surface layer (ES-2), Biocoral(Inoteb, France), and Bio-Oss(Geistlich Pharma, Wolhusen, Switzerland). After 4 and 8 weeks of healing, histomorphometic analysis was performed to evaluate the amount of newly formed mineralized bone area (NB%). Results: Histologic and histomorphometric analysis showed new bone formation and direct bony contact with the grafted materials in all groups. At 4 weeks, Biocoral group showed greater NB% compared to Bio-Oss and ES-1 groups (P<0.05). At 8 weeks, Biocoral and ES-2 groups showed significantly greater NB% compared to Bio-Oss group (P<0.05). Conclusion: These results indicate that natural calcium carbonate-derived bone substitutes with microporous calcium-deficient hydroxyapatite surface layer may be an effective materials treating osseous defects.

• Mycobiology
• /
• 제46권4호
• /
• pp.429-439
• /
• 2018

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the trpC and crp promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.