• Asian-Australasian Journal of Animal Sciences
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• 제31권9호
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• pp.1507-1515
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• 2018

Objective: In the poultry industry, the most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate the regulatory pathways during muscle differentiation. To gain insight of muscle differentiation mechanism during growth period, we identified and validated calcium-related genes which were highly expressed during muscle differentiation through mRNA sequencing analysis. Methods: We conducted next-generation-sequencing (NGS) analysis of mRNA from undifferentiated QM7 cells and differentiated QM7 cells (day 1 to day 3 of differentiation periods). Subsequently, we obtained calcium related genes related to muscle differentiation process and examined the expression patterns by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Results: Through RNA sequencing analysis, we found that the transcription levels of six genes (troponin C1, slow skeletal and cardiac type [TNNC1], myosin light chain 1 [MYL1], MYL3, phospholamban [PLN], caveolin 3 [CAV3], and calsequestrin 2 [CASQ2]) particularly related to calcium regulation were gradually increased according to days of myotube differentiation. Subsequently, we validated the expression patterns of calcium-related genes in quail myoblasts. These results indicated that TNNC1, MYL1, MYL3, PLN, CAV3, CASQ2 responded to differentiation and growth performance in quail muscle. Conclusion: These results indicated that calcium regulation might play a critical role in muscle differentiation. Thus, these findings suggest that further studies would be warranted to investigate the role of calcium ion in muscle differentiation and could provide a useful biomarker for muscle differentiation and growth.

• 한국지반신소재학회논문집
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• 제12권1호
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• pp.29-38
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• 2013

본 논문에서는 생분해 배수재를 이용한 오염토양 정화기술의 현장적용성을 평가하기 위하여 생분해 배수재의 물리적 특성시험을 수행하였으며, 이를 이용한 현장실험을 통하여 중금속 오염토양의 정화효율을 분석하였다. 두 종류(실린더 코어형, 하모니카형)의 생분해 배수재에 대한 시험결과, 실린더 코어형 배수재의 경우, 한국산업규격 표준시방에서 제시하는 기준치를 모두 만족하였으며, 하모니카형 생분해성 배수재는 실린더 코어형 배수재에 비해 배수성능이 상당히 낮은 결과를 나타내었다. 또한 오염토양 복원시 사용한 세정제로는 자체독성이 적어 생태계에 미치는 영향을 최소화하며, 생물학적 재순환시스템과의 최적인 조건을 지니고 있는 시트르산(ctric acid)을 선정하여 중금속 중 Cd, Cu, Pb을 현장실험 대상 오염물질로 선정 후 실험을 수행하였다. 현장실험은 3가지 설치조건을 고려하여 정화효율을 분석하였으며, 반응물질이 도포된 생분해 배수재를 이용하여 세척제를 주입 추출을 동시에 수행한 경우가 복원효율이 가장 높은 것으로 평가되었다.

• 대한수의학회지
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• 제45권2호
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• pp.155-161
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• 2005

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1863-1870
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• 2016

Antimicrobial peptides/proteins (AMPs) are present in all types of organisms, from microbes and plants to vertebrates and invertebrates such as insects. The grasshopper Oxya chinensis sinuosa is an insect species that is widely consumed around the world for its broad medicinal value. However, the lack of available genetic information for this species is an obstacle to understanding the full potential of its AMPs. Analysis of the O. chinensis sinuosa transcriptome and expression profile is essential for extending the available genetic information resources. In this study, we determined the whole-body transcriptome of O. chinensis sinuosa and analyzed the potential AMPs induced by bacterial immunization. A high-throughput RNA-Seq approach generated 94,348 contigs and 66,555 unigenes. Of these unigenes, 36,032 (54.14%) matched known proteins in the NCBI database in a BLAST search. Functional analysis demonstrated that 38,219 unigenes were clustered into 5,499 gene ontology terms. In addition, 26 cDNAs encoding novel AMPs were identified by an in silico approach using public databases. Our transcriptome dataset and AMP profile greatly improve our understanding of O. chinensis sinuosa genetics and provide a huge number of gene sequences for further study, including genes of known importance and genes of unknown function.

• 한국융합학회논문지
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• 제9권2호
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• pp.303-311
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• 2018

스쿼트운동은 안전하고 효과적인 운동성과를 기대할 수 있는 중요한 프리웨이트운동의 하나로 하체강화운동에 가장 효과적인 운동으로 알려져 있다. 인터렉티브 스쿼트운동 장치를 활용하여 동적인 운동과정을 단순화하여 스쿼트 운동 동작을 모델링하고 이를 기반으로 한 생체신호 및 동작 분석을 수행할 수 있으며 또한 장치를 통해 사용자 자세교정을 통해 올바르고 효과 높은 스쿼트운동 과정을 확립할 수 있다. 제안된 모델링을 기반으로 설계된 풋플레이트에 위치한 로드셀의 검출된 신호를 활용하여 스쿼트동작을 생체신호검출과 검출된 신호처리과정을 거쳐 스쿼트운동의 오류동작을 검출해 내는 신호출력을 확보할 수 있다. 본 논문에서 제안된 인터랙티브 스쿼트운동장치의 설계방법과 동작의 안전성 분석방법은 생체역학 신호처리 방법에 기인하며 사용자의 스쿼트 운동자세를 해석을 위한 검출된 생체신호 및 동작신호를 통해 올바르지 못한 스쿼트 동작을 교정함으로서 바른 스쿼트 운동을 유도할 수 있으며 연구 결과를 VR 장치에 적용하거나 운동 평가를 위한 장치로 활용할 수 있다.

• Genomics & Informatics
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• 제6권3호
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• pp.142-146
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• 2008

In order to understand the protein functions that are related to disease, it is important to detect the correlation between amino acid mutations and disease. Many mutation studies about disease-related proteins have been carried out through molecular biology techniques, such as vector design, protein engineering, and protein crystallization. However, experimental protein mutation studies are time-consuming, be it in vivo or in vitro. We therefore performed a bioinformatic analysis of known disease-related mutations and their protein structure changes in order to analyze the correlation between mutation and disease. For this study, we selected 111 diseases that were related to 175 proteins from the PDB database and 710 mutations that were found in the protein structures. The mutations were acquired from the Human Gene Mutation Database (HGMD). We selected point mutations, excluding only insertions or deletions, for detecting structural changes. To detect a structural change by mutation, we analyzed not only the structural properties (distance of pocket and mutation, pocket size, surface size, and stability), but also the physico-chemical properties (weight, instability, isoelectric point (IEP), and GRAVY score) for the 710 mutations. We detected that the distance between the pocket and disease-related mutation lay within $20\;{\AA}$ (98.5%, 700 proteins). We found that there was no significant correlation between structural stability and disease-causing mutations or between hydrophobicity changes and critical mutations. For large-scale mutational analysis of disease-causing mutations, our bioinformatics approach, using 710 structural mutations, called "Structural Mutatomics," can help researchers to detect disease-specific mutations and to understand the biological functions of disease-related proteins.

• 대한의용생체공학회:의공학회지
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• 제25권6호
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• pp.545-550
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• 2004

디지털 내시경 영상에서 식도염 등의 이상부위를 검출하기 위하여 임상 영상의 색상과 텍스쳐 인자에 대한 정보를 얻은 후 판별분석에 의해 영상의 이상부위론 인식하 수 있는 영상처리 알고리즘을 개발하였다. 이 알고리즘을 개발하기 위하여 여러 가지 영상처리 인자들 중에서 어떠한 인자들이 정상과 이상 부위를 구별할 수 있는 중요한 특징 인자가 되는지를 구명하였다. 이 특징 인자들을 이용하여 식도염의 중요한 진단기준이라 할 수 있는 미란 및 궤양에 대한 검출을 수행하였다. 이를 검증하기 위하여 20개의 영상 이미지를 사용하였으며 판별분석의 알고리즘을 사용할 때 보정단계와 검증단계의 성공률은 각각 92.8%와 92.4%를 나타내었다.

• Journal of Biosystems Engineering
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• 제38권1호
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• pp.33-40
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• 2013

Purpose: The goal of this study was to build an accurate digital factory that evaluates the performance of a factory using computer simulation. To achieve this goal, we evaluated the effect of worker-related variables on production in a simulation model using comparative analysis of two cases. Methods: The overall work process and worker-related variables were determined and used to build a simulation model. Siemens PLM Software's Plant Simulation was used to build a simulation model. Also, two simulation models were built, where the only difference was the use of the worker-related variable, and the total daily production analyzed and compared in terms of the individual process. Additionally, worker efficiency was evaluated based on worker analysis. Results: When the daily production of the two models were compared, a 0.16% error rate was observed for the model where the worker-related variables were applied and error rate was approximately 5.35% for the model where the worker-related variables were not applied. In addition, the production in the individual processes showed lower error rate in the model that included the worker-related variables than the model where the worker-related variables were not used. Also, among the total of 22 workers, only three workers satisfied the IFRS (International Financial Reporting Standards) suggested worker capacity rate (90%). Conclusions: In the daily total production and individual process production, the model that included the worker-related variables produced results that were closer to the real production values. This result indicates the importance of worker elements as input variables, in regards to building accurate simulation models. Also, as suggested in this study, the model that included the worker-related variables can be utilized to analyze in more detail actual production. The results from this study are expected to be utilized to improve the work process and worker efficiency.

• Mass Spectrometry Letters
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• 제12권4호
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• pp.192-199
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• 2021

Alpine glaciers harbor a large quantity of bio-labile dissolved organic matter (DOM), which plays a pivotal role in global carbon cycling as glacial-fed streams are headwaters of numerous large rivers. To understand the complexity, origin, and fate of DOM in glaciers and downstream-linked streams and lakes, we elucidated the molecular composition of DOM in two different Tibetan Plateau glaciers, eight glacial-fed streams and five lakes, using an ultrahigh-resolution 15 Tesla Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The compositional changes of the DOM samples revealed that glacier DOM mostly exhibited sulfur-containing organic compounds (CHOS species). We also found that aliphatic formulae contributed more than 50% of the total abundance of assigned molecules in glacier samples, and those compounds were significantly related to CHOS species. The CHO proportions of glacial-fed streams and lakes samples increased with increasing distance from glacial terminals. The relative contribution of terrestrial-derived organics (i.e., lignins and tannins) declined while microbial-originated organics (aliphatics) increased with increasing elevation. This suggested the gradual input of allochthonous materials from non-glacial environment and the degradation of microbe-derived compounds along lower elevations. Alpine glaciers are retreating as a result of climate change and they nourished numerous streams, rivers, and downstream-linked lakes. Therefore, the interpretations of the detailed molecular changes in glacier ice, glacial-fed streams, and alpine lakes on the Tibetan Plateau could provide broad insights for understanding the biogeochemical cycling of glacial DOM and assessing how the nature of DOM impacts fluvial ecosystems.

• 한국육종학회지
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• 제40권4호
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• pp.361-370
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• 2008

In order to better understand the morphological differentiation of the two cultivated types of Perilla crop and their weedy types in Korea and Japan, we studied the variation of 62 accessions by examining 15 morphological characteristics. By using ANOVA (one-way analysis of variance), we determined that var. frutescens and var. crispa showed significant morphological differences in terms of plant height and seed weight. Furthermore, cultivated var. frutescens and var. crispa could also be clearly discriminated from one another using PCA (principal component analysis). Specifically, quantitative and qualitative characteristics such as plant height, seed weight, degree of pubescence, shape of leaf, color of leaf, fragrance of plant, color of flower, color of stem and seed size greatly contributed to differences seen in the positive and negative direction on the first axis. In our study, most accessions of cultivated var. frutescens and those of its weedy type could be clearly discriminated from one another, however, most accessions of cultivated and weedy types of var. crispa were not clearly discriminated by the ANOVA and PCA analyses. These results indicated that cultivated var. frutescens can be considered to be a domesticated form, while the cultivated var. crispa can not be considered to be a domesticated form in Korea and Japan. It is our belief that our results concerning the morphological variations among cultivated types of Perilla crop and their weedy types in Korea and Japan will help ensure the long-term success of breeding programs and maximize the use of the germplasm resources in Korea.