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Proteome analysis of chickens fed with tissue culture medium waste after harvest of Korean wild ginseng  

Seol, Jae-Won (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University)
Hwang, In Ho (National Livestock Research Institute)
Chae, Joon-Seok (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University)
Kang, Hyung-Sub (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University)
Ryu, Kyeong-Seon (Department of Animal Resources and Biotechnology, Chonbuk National University)
Kang, Chun-Seong (E&T Co. Ltd.)
Park, Sang-Youel (Bio-Safty Research Institute, College of Veterinary Medicine, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.45, no.2, 2005 , pp. 155-161 More about this Journal
Abstract
Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).
Keywords
Proteomic analysis; Chicken; Gienseng; MALDI-TOF;
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