• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.54-60
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• 2006

In this paper, we describe a bio-fluidic device for adaptive sample pretreatment, in order to optimize the conditions under which absorbance assays can be conducted. This device can be successfully applied to the measurement of Escherichia coli (E. coli) concentrations using adaptive dilution, with which the dilution ratio can be adjusted during the dilution. Although many attempts have been previously made to miniaturize complex biochemical analyses at the chip scale, very few sample pretreatment processes have actually been miniaturized or automated at this point. Due to the lack of currently available on-chip pretreatments, analytical instruments tend to suffer from a limited range of analysis. This occasionally hinders the direct and quantitative analysis of specific analyses obtained from real samples. In order to overcome these issues, we exploit two novel strategies: dilution with a programmable ratio, and to-and-fro mixing. The bio-fluidic device consists of a rectangular chamber constructed of poly(dimethylsiloxane) (PDMS). This chamber has four openings, an inlet, an outlet, an air control, and an air vent. Each of the dilution cycles is comprised of four steps: detection, liquid drain, buffer injection, and to-and-fro mixing. When using adaptive sample pretreatment, the range in which E. coli concentrations can be measured is broadened, to an optical density (O.D.) range of $0.3{\sim}30$. This device may prove useful in the on-line monitoring of cell concentrations, in both fermenter and aqueous environments.

• 대한의용생체공학회:의공학회지
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• 제31권5호
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• pp.375-384
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• 2010

The purpose of this study is to determine whether there is a correlation between the segmental bio-impedance measurement with the frequency modulations and the life-style disease of obesity. An obesity is not simply the factor for estimating the life-style disease of obesity, but also the risk factor occurring. There are many methods (BMI, WHR, Waist, CT, DEXA, BIA, etc.) for measuring a degree of obesity; the bio-impedance measurement is more economic and more effective than others. The physical examination, the blood test, the medical imaging diagnosis and the bio-impedancemeasurementswithmultiple frequencies for each body parts have been conducted for 77 people. The estimated value has been calculated through a segmental bio-impedance model based on multi-frequency that was created to reflect the highest correlation by analyzing correlation with linear regression analysis method for the measured bio-impedance and the risk factors. Then we compared with the clinical diagnosis. In case of high level cholesterol, low HDL-C and high LDL-C for life-style disease, the sensitivity is 80~100%and the specificity is 83~100%. This study has shown conclusively that bio-impedance can be a possible predictor to analyze the disease risk rate of population and individual health maintenance. And also the multi-frequency segmental bio-impedance can be used as early predictor to estimate the life-style disease of obesity.

• Journal of Microbiology and Biotechnology
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• 제23권11호
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• pp.1598-1609
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• 2013

Organochlorine pesticide residues continue to remain as a major environmental threat worldwide. Lindane is an organochlorine pesticide widely used as an acaricide in medicine and agriculture. In the present study, a new lindane-degrading yeast strain, Pseudozyma VITJzN01, was identified as a copious producer of glycolipid biosurfactant. The glycolipid structure and type were elucidated by FTIR, NMR spectroscopy, and GC-MS analysis. The surface activity and stability of the glycolipid was analyzed. The glycolipids, characterized as mannosylerythritol lipids (MELs), exhibited excellent surface active properties and the surface tension of water was reduced to 29 mN/m. The glycolipid was stable over a wide range of pH, temperature, and salinity, showing a very low CMC of 25 mg/l. Bio-microemulsion of olive oil-in-water (O/W) was prepared using the purified biosurfactant without addition of any synthetic cosurfactants, for lindane solubilization and enhanced degradation assay in liquid and soil slurry. The O/W bio-microemulsions enhanced the solubility of lindane up to 40-folds. Degradation of lindane (700 mg/l) by VITJzN01 in liquid medium amended with bio-microemulsions was found to be enhanced by 36% in 2 days, compared with degradation in 12 days in the absence of bio-microemulsions. Lindane-spiked soil slurry incubated with bio-microemulsions also showed 20-40% enhanced degradation compared with the treatment with glycolipids or yeast alone. This is the first report on lindane degradation by Pseudozyma sp., and application of bio-microemulsions for enhanced lindane degradation. MEL-stabilized bio-microemulsions can serve as a potential tool for enhanced remediation of diverse lindane-contaminated environments.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 1997년도 가을 심포지움 및 학술논문발표요지
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• pp.20-26
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• 1997

The design or analysis of beehouse inside temperature environment based on steady heat transfer theory causes much deviation and theoretically it is impossible to control the inside temperature lower than the outside temperature under the condition that the bee produces heat and no cooling equipment is installed. But in practical use of beehouse, the inside temperature is somehow lower than the outside temperature because of the heat inertia of concrete floor. (omitted)

• 정보처리학회논문지B
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• 제11B권7호
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• pp.759-768
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• 2004

생체인식기술을 응용한 제품이 다양해지면서 상호운용성의 문제가 제기되어 국제표준화가 진행중인 BioAPl(Biometric Application Pro-gramming Interface)를 기반으로 한 생체인식 제품의 개발이 증대되고 있다. 그러나 BioAPl를 적용하여 개발된 제품에 대한 성능 측정 및 표준규격의 적합성 여부에 대한 신뢰성 있는 평가 도구가 마련되어 있지 않은 상태이다. 본 논문은 생체인식시스템이 BioAPl 규격의 요구사항 및 기준에 얼마나 만족하여 개발하였는가를 검증하기 위한 적합성시험 방법을 연구하였다. 제안된 적합성 시험기술의 첫 번째는 BioAPl명세서가 제공하는 각 함수들을 제대로 구현하였는가에 대한 확인과 사용여부를 평가하는 것이다. 이것은 응용에서 APl(Application Provider Inter-face)함수를 호출하면 프레임워크를 통해 BSP(Biometric Service Provider)를 실행하는 것으로 이때 파라미터와 선행함수의 선택이 필요하다. 두 번째는 BioAPl의 해당 테스트케이스를 분석하여 모듈관리, 핸들기능, 검증기능에 대한 시나리오를 평가하는 것이다. 실험은 BioAPl 컨소시엄에서 제공하는 샘플프로그램과 상용 지문검증시스템의 BSP를 사용하여 제안하는 적합성 평가 방법에 대한 실험을 수행하였다. 이에 따라 BioAPl를 기반으로 한 BSP들이 요구사항에 적합하게 개발되었는지를 판단할 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제27권9호
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• pp.1329-1334
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• 2006

The electrochemical reduction of coumarin, 7-acetoxy-4-methyl coumarin (AMC), and 7-acetoxy-4-bromomethyl coumarin (ABMC), in 0.1 M tetraethyl ammonium perchlorate/acetonitrile solution was carried out by direct current, differential pulse polarography, cyclic voltammetry, and controlled potential coulometry. The electrochemical reduction of ABMC was proceeded through three steps of electron transfer coupled with the chemical reactions. The color of solution was changed to yellow when the carbonyl group was reduced during 2nd step (-1.8 volts) and independented with cleavage of bromo group. Highest fluorescence intensity showed when the electrochemical reduction of AMC was controlled at near the potential (-2.3 volts vs. Ag/AgCl).

• Structural Monitoring and Maintenance
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• 제5권3호
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• pp.325-343
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• 2018

This study aims to propose a performance-based design method of a novel passive base isolation system, BIO isolation system, which is inspired by an energy dissipation mechanism called 'sacrificial bonds and hidden length'. Fragility functions utilized in this study are derived, indicating the probability that a component, element, or system will be damaged as a function of a single predictive demand parameter. Based on PEER framework methodology for Performance-Based Earthquake Engineering (PBEE), a systematic design procedure using performance and fragility objectives is presented. Base displacement, superstructure absolute acceleration and story drift ratio are selected as engineering demand parameters. The new design method is then performed on a general two degree-of-freedom (2DOF) structure model and the optimal design under different seismic intensities is obtained through numerical analysis. Seismic performances of the biologically inspired (BIO) isolation system are compared with that of the linear isolation system. To further demonstrate the feasibility and effectiveness of this method, the BIO isolation system of a 4-storey reinforced concrete building is designed and investigated. The newly designed BIO isolators effectively decrease the superstructure responses and base displacement under selected earthquake excitations, showing good seismic performance.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.108-108
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• 2017

In this study, biocomposite filaments with agricultural by-products can be used in extrusion-based 3D (Three-dimensional) printing. Extrusion-based 3D printing stands as a promising technique owing to its versatility. We hypothesized that bio-filament composite consisted of something derived from agricultural by-products could be used as 3D printing materials that could overcome the drawbacks of PCL (poly-caprolactone). Bio-filament mixed with PCL and agricultural by-products was defined as r-PCL in this study. In order to find it out the optimal mixing ratio of filaments, we had investigated PCL, r-PCL 10%, r-PCL 20%, r-PCL 50% separately. The morphological and chemical characteristics of the filaments were analyzed by FE-SEM (Field emission scanning electron microscope) and EDX (Energy-dispersive X-Ray spectroscopy), and the mechanical properties were evaluated by stress-strain curve, water contact angle, and cytotoxicity analysis. Results of this study have been shown as a promising way to produce eco-friendly bio-filaments composite for FDM (Fused deposition modeling) method based 3D printing technology. Thus, we could establish biomimetic scaffolds based on bio-printer filaments mixed with agricultural by-product.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.109.1-109
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• 2003

From the PMMV (pepper mild mottle virus)-inducible ESTs differentially expressed in Capsicum chinense PI257284, we isolated a full-length cDNA (CcPHZF： Capsicum chinense phenazine), encoding a phenazine biosynthesis protein which catalyzes the hydroxylation of phenozine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. Phenazine compound has been known to exhibit broad-spectrum of antibiotic activity against various species of bacteria and fungus. The entire region of CcPHZF is 879 bp in length and the open reading frame predicted a polypeptide of 292 amino acids. The homolog of CcPHZF is not Present in database except clones of AC004044 and NM100203 from Arabidopsis with 58 and 59％, respectively. Genomic Southern analysis indicated that the pepper genome contains a single copy of CcPHZF. The CcPHZF was strongly induced in the pepper leaves 3 days after PMMV treatment, when HR occurs on the leaf surface. Characterization of CcPHZF is underway to investigate if the CcPHZF is related to disease resistance against pathogens.

• Journal of Pharmaceutical Investigation
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• 제34권1호
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• pp.73-79
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• 2004

The union of information-technology and biology presents great possibilities to both applications of bio-information and development of science and technology. Also, meaningful analysis of bio-information brings about a new innovation in the field of bio-market with the advent and growth of bioinformatics. Hence, bioinformatics is the most import aspect for establishing a science-technology-oriented society in the $21^{st}$ century. This article provides trends in current state of bioinformatics. Technological development of bioinformatics for the rapid growth of bio-industry means that using bioinformatics, a biologist can process and store enormous amount of data such as current Human Genome Project and future data in the field of biology. We have manly looked at the tends of bio-information, databases and mining tools that are generally used, and strategies and directions for the future.