• Analytical Science and Technology
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• v.23 no.6
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• pp.544-550
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• 2010

Mistaking pink colored thermal oil for grape wine, a victim drank the oil to death which was analyzed to contain 39% of ethylene glycol. Thermal oil could be used for heat transfer to prevent the malfunction due to the high pressure in the boiler operated at high temperature when using water. Main component of thermal oil is known to be mineral oil or ethylene glycol. From the blood and other tissue of the victim from autopsy, ethylene glycol and its metabolite were simultaneously analyzed by GC/MS after extraction under acidic condition with acetonitrile followed by derivatization with BSTFA. About 0.2 g of the specimens were pretreated with 50 uL of 0.5 M HCl solution to keep acidic condition, then dehydrated with anhydrous sodium sulfate followed by concentration under nitrogen stream. Ethylene glycol and glycolic acid concentration in blood was measured to be $2,755\;{\mu}g/mL$ and $174\;{\mu}g/mL$ respectively. In other specimen, the concentration of ethylene glycol and glycolic acid was $860\;{\mu}g/g\sim1,290\;{\mu}g/g$ and $93\;{\mu}g/g\sim134\;{\mu}g/g$. Especially, crystal appeared in kidney which was supposed xalate from the metabolite of ethylene glycol.

• Journal of Food Hygiene and Safety
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• v.22 no.1
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• pp.63-75
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• 2007

A survey on sanitary management at 98 food service institutions located in Jeollabuk-Do were conducted. Food service institutions included 13 hospitals, 38 schools, 40 enterprises, and 7 others. The sterilization in boiling water was used for disinfection of tableware, spoon and chopsticks, and dish towel. Cooking kit, refrigerator, chopping board, and knife were sterilized with disinfectant. Frequency of sanitary training program on employees was 56.1% for 1 time/month, 12.2% for 2-3 times/month, and 11.3% for 1 time/week. Preparing ratio of defrosting, warehouse and rest room, and washing and sterilization diary were 73.8, 60.5, and 54.5%, respectively. However, checking ratio of environments (9.5%), personal sanitation (10.8%), and sanitary training program diary(8.4%) were low. Major obstacle factors on the introduction of Hazard analysis critical control point (HACCP) system into food service institutions were lack of equipment and facilities (54.1%) and lack of understanding and data on HACCP system (17.3%). Therefore, in order to reduce the outbreak of food-borne diseases increasing frequency, we estimated that the introduction of sanitary management system and the conversion of dietician' perception on sanitary managements in food service institutions were needed.

• Journal of Food Hygiene and Safety
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• v.31 no.4
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• pp.278-285
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• 2016

This study used response surface methodology (RSM) in an effort to optimize the ultrasoundassisted extraction condition of Camellia sinensis L. and Artemisia argyi mixture in order to increase extraction yield in the extract. The effects of three independent variables, $X_1$ (Mixture ratio, 60-80%), $X_2$ (Ratio of water to raw material, 20~100 mL/g), and $X_3$ (Extraction time, 25-145 min), were investigated at three levels using Box-Behnken design (BBD) to obtain the highest extraction efficiency. Y (Extraction yield) was chosen as dependent variable. Our result showed that the coefficient of determination ($R^2$) of the model was 0.9747, with significant at the level of p < 0.002. Furthermore, the predicted values of each variable were similar to the actual values. The optimum extraction conditions were as follows: mixture ratio of 85.86%, ratio of water to raw material of 92.73 mL/g, and extraction time of 56.52 min. At these conditions, predicted extraction yield was 30.03%. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing extraction conditions of Camellia sinensis L. and Artemisia argyi mixture.

• Applied Chemistry for Engineering
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• v.25 no.4
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• pp.401-408
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• 2014

In membrane bio-reactor (MBR), the aeration control is one of the important independent variables to decrease fouling and to save energy with shear stress change on the membrane surface. The paper was carried out for numerical simulation of 3-dimensional fluid flow phenomena of the cylindrical bioreactor with submerged flat membranes equipped in the center and supplied the air from the bottom by using the COMSOL program. The viscosity and temperature of solution were assumed to be constant, and the specific air demand based on permeate volume ($SAD_p$) defined as scouring air per permeate rates was used as a variable. The calculated CFD velocities were compared with those of the velocity meter measurement and video image analysis, respectively. The results were good agreement each other within 11% error. For fluid flow in the reactor the liquid velocity increased rapidly between the air diffuser and membrane module, but the velocity decreased during flowing of the membrane module. Also, the velocity increased as it was near from the reactor wall to the central axis. The calculated shear stress on the membrane surface showed the highest value at the center part of the module bottom side and increased as aeration rate increased. Especially, the wall shear stress increased dramatically as the aeration rate increased from 0.15 to 0.25 L/min.

• Journal of Life Science
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• v.23 no.1
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• pp.84-94
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• 2013

The cytotoxicity of coffee-like green tea (CLGT) was determined in a human breast cancer cell line, MCF-7; a human prostate cancer cell clone, PC-3; a human neuroblastoma cell line, SK-N-SH; and a rat cardiomyoblast cell line, H9c2, with reference to green tea leaves (GTL). The CLGT was prepared by roasting the GTL for 60 min at $240^{\circ}C$ in a temperature-controlled frying pan. The CLGT preparation imitated the flavor and taste characteristics of coffee fairly well according to sensory analysis. The CLGT preparation had no adverse cytotoxic effects on the cancer cells or the normal cells compared to GTL. No significant change in the antioxidant activity was seen in the CLGT preparation compared to that of GTL. The amount of total protein, sugar, and phenolic compounds was reduced in the preparation relative to those in GTL, a fact that might explain the coffee-like flavor and/or taste characteristics of the CLGT preparation. These results suggest that CLGT prepared by roasting GTL for 60 min at $240^{\circ}C$ does not show any adverse effects on cancer cells and normal cells compared to GTL. They imply that CLGT could be safe for human consumption.

• Journal of Life Science
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• v.27 no.7
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• pp.834-839
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• 2017

Propolis is a natural product collected from plants by honey bees product used extensively in traditional medicine for its antioxidant, anti-inflammatory, immunomodulatory and anti-cancer effects. Propolis exhibits a broad spectrum of biological activities because it is a complex mixture of natural substances. Ovarian cancer is the second most common newly diagnosed cancer from all cancers among women in Korea and the leading cause of death from gynecological malignancies. While most ovarian cancer patients initially respond to surgical debulking and chemotherapy, patients later succumb to the disease. Thus, there is an urgent need to test novel therapeutic agents to counteract the high mortality rate associated with ovarian cancer. In this study, we investigated the anti-cancer properties and the active mechanism of Australian propolis in human epithelial ovarian cancer A2780 cells. Our data revealed that propolis showed a cytotoxic activity in a dose-dependent manner. Flow cytometric analysis for cell cycle arrest and apoptosis using propidium iodide staning and annexin V-FITC indicated that propolis could induce cycle arrest in the G0/G1 phase and apoptosis in a dose-dependent manner on human epithelial ovarian cancer cells. These results suggest that the Australian propolis is potential alternative agent on ovarian cancer prevention and treatment.

• Food Science of Animal Resources
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• v.37 no.1
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• pp.1-9
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• 2017

The rotavirus-induced diarrhea of human and animal neonates is a major public health concern worldwide. Until recently, no effective therapy is available to specifically inactivate the rotavirion particles within the gut. Passive immunotherapy by oral administration of chicken egg yolk antibody (IgY) has emerged of late as a fresh alternative strategy to control infectious diseases of the alimentary tract and has been applied in the treatment of diarrhea due to rotavirus infection. The purpose of this concise review is to evaluate evidence on the properties and performance of anti-rotavirus immunoglobulin Y (IgY) for prevention and treatment of rotavirus diarrhea in human and animal neonates. A survey of relevant anti-rotavirus IgY basic studies and clinical trials among neonatal animals (since 1994-2015) and humans (since 1982-2015) have been reviewed and briefly summarized. Our analysis of a number of rotavirus investigations involving animal and human clinical trials revealed that anti-rotavirus IgY significantly reduced the severity of clinical manifestation of diarrhea among IgY-treated subjects relative to a corresponding control or placebo group. The accumulated information as a whole depicts oral IgY to be a safe and efficacious option for treatment of rotavirus diarrhea in neonates. There is however a clear need for more randomized, placebo controlled and double-blind trials with bigger sample size to further solidify and confirm claims of efficacy and safety in controlling diarrhea caused by rotavirus infection especially among human infants with health issues such as low birth weights or compromised immunity in whom it is most needed.

• Journal of Korea Society of Industrial Information Systems
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• v.16 no.2
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• pp.19-29
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• 2011

Due to the development of web technologies and the increasing use of smart devices such as smart phone, in recent various web services are widely used in many application fields. In this environment, the topic of supporting personalized and intelligent web services have been actively researched, and an analysis technique on a web-click stream generated from web usage logs is one of the essential techniques related to the topic. In this paper, for efficient analyzing a web-click stream of sequences, a sequential pattern mining technique is proposed, which satisfies the basic requirements for data stream processing and finds a refined mining result. For this purpose, a concept of interesting sequential patterns with a time-interval constraint is defined, which uses not on1y the order of items in a sequential pattern but also their generation times. In addition, A mining method to find the interesting sequential patterns efficiently over a data stream such as a web-click stream is proposed. The proposed method can be effectively used to various computing application fields such as E-commerce, bio-informatics, and USN environments, which generate data as a form of data streams.

• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.914-917
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• 2003

This study was undertaken to find the optimum condition for the Maillard browning reaction of tagatose and glycine model solution by applying the response surface methodology. Independent variables were pH (3, 5, 7), temperature (70, 85, $100^{\circ}C$), and time (60, 180, 300 min), while the dependent variables were absorbance, yellowness, color difference, and organoleptic score. The quadratic models with the cross-product proved to be suitable, due to the high coefficients of determination and the lack of fit results. Since all the dependent variables had saddle points, the optimal points were determined through ridge analysis. For absorbance, yellowness, and color difference, the optimal points were the lowest values; in contrast, the optimal point of organoleptic score was the highest value.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.2
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• pp.107-116
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• 2006

Purpose:Maxillay sinus grafting is an effective treatment procedure to improve bone height in the posterior maxillar area for implant installation. Beta-tricalciumphosphate(${\beta}$-TCP) was introduced to be grafting substitute material, providing a reasonable bio-degradation time, no need for harvesting procedure. The purpose of this study is to evaluate bone healing & regeneration phase using histomorphometric and immunohistochemical analysis. Material & Methods:Sixteen rabbits were divided into 4 groups. Bi-lateral maxillary sinus membranes were elevated at each rabbits, ${\beta}$-TCP was augmented in left sinus, autogenous bone was augmented in right sinus. The rabbits were sacrificed at 2, 4, 8 and 12 weeks. We investigated the bone regeneration & growth factor expression. Results: 1. The mean new bone volume formation was 28.99${\pm}$6.55%, 49.54${\pm}$5.47%, 69.09${\pm}$8.90% in autogenous grafted area, and 22.86${\pm}$5.56%, 24.00${\pm}$4.09%, 34.11${\pm}$3.37% in ${\beta}$-TCP area at 4, 8, 12 weeks. Therefore, new bone formation in autogenous bone was significantly higher than ${\beta}$-TCP (p<0.05). 2. The BMP 2/4 expression in autogenous bone grafted area was higher at 4, 8 weeks. 3. There was no difference in expression pattern of BMP-7/PDGF/VEGF during grafted bone regeneration. Conclusion:The authors we conclude that the autogenous bone graft was faster than ${\beta}$-TCP in bone regeneration, and the BMP 2/4 were more important in graft bone regeneration.