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http://dx.doi.org/10.13103/JFHS.2016.31.4.278

Optimization of Extraction Conditions for Mixture of Camellia sinensis L. and Artemisia argyi by Response Surface Methodology  

Kim, Young-Hyun (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Woo-Sik (G1-Bio Co. Ltd.)
Kim, Jae-Min (Department of Food Science and Biotechnology, Kangwon National University)
Choi, Sun-il (Department of Food Science and Biotechnology, Kangwon National University)
Jung, Tae-Dong (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Jong-Dai (Department of Food Science and Biotechnology, Kangwon National University)
Lim, Jae Kag (Department of Chemical Engineering and Biotechnology, Korea Polytechnic University)
Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.4, 2016 , pp. 278-285 More about this Journal
Abstract
This study used response surface methodology (RSM) in an effort to optimize the ultrasoundassisted extraction condition of Camellia sinensis L. and Artemisia argyi mixture in order to increase extraction yield in the extract. The effects of three independent variables, $X_1$ (Mixture ratio, 60-80%), $X_2$ (Ratio of water to raw material, 20~100 mL/g), and $X_3$ (Extraction time, 25-145 min), were investigated at three levels using Box-Behnken design (BBD) to obtain the highest extraction efficiency. Y (Extraction yield) was chosen as dependent variable. Our result showed that the coefficient of determination ($R^2$) of the model was 0.9747, with significant at the level of p < 0.002. Furthermore, the predicted values of each variable were similar to the actual values. The optimum extraction conditions were as follows: mixture ratio of 85.86%, ratio of water to raw material of 92.73 mL/g, and extraction time of 56.52 min. At these conditions, predicted extraction yield was 30.03%. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing extraction conditions of Camellia sinensis L. and Artemisia argyi mixture.
Keywords
Response surface methodology; Ultrasound-assisted extracted; Box-Behnken design; Camellia sinensis L.; Artemisia argyi;
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Times Cited By KSCI : 17  (Citation Analysis)
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