• Journal of the Korean Society of Clothing and Textiles
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• v.42 no.3
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• pp.516-529
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• 2018

This study investigated the dyeing properties and bio-functions of cotton fabrics dyed with naturally fermented Ecklonia cava extract in order to compare it with a comparison of unfermented extract. Hot water-extracted Ecklonia cava was fermented naturally under the various conditions of a fermenting period (2-8 days) and amount of molasses (0.1-1.8% v/v); in addition, it was also tested for characterization by FT-IR, antioxidant activity, total polyphenol content, and anti-microbial activity. For dyed cotton fabrics, color strength (K/S), physical color properties, dyeing fastness, sun protective property, and anti-microbial activity were evaluated considering dyeing conditions. As a result, the fermented dye under fermentation condition of 0.1% v/v with molasses during 4 days was revealed as having a similar chemical structure to the unfermented one and showed a total polyphenol content with 32.88mg/g and better antioxidant activity than the unfermented one. As for dyed fabrics, the color strength value by K/S was the highest under the condition of 0.1% v/v of molasses during 4 days among all fermenting conditions. The dyed fabrics had a reasonably good fastness (except for light). Anti-microbial activity against K. pneumoniae was better for the fermented extract-dyed fabric especially with lower dye concentrations.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.2
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• pp.420-429
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• 2004

It has been needed diagnosis technology of the EFG(Electro Functio Gram) measurement concept which is incorporated characteristic of modern science and biomedical engineering, because function abnormality diagnosis can't diagnose with visualization instruments or clinical pathology. The general diagnosis system(EFG system) has been developed to use basic diagnosis instrument that has reappearance, reliance and convenience using the functio diagnosis technology, in the field of western/oriental medicine. Also, we improved sticking electrode and simultaneous measurement method of the limbs 8CH, head 2CH to diagnose body circulation of Qi and acupoints for Oriental Medicine diagnosis/therapy. The result of clinical for adult man 20 persons, the EFG system can diagnose state of 12 meridians and autonomic nervous system. Therefore, in this paper, we designed of oriental medicine diagnosis system by bio-electric response with materialized H/W and S/W for 12 meridians state diagnosis of human body and system construction of the EFG.

• Korean Journal of Poultry Science
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• v.32 no.1
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• pp.23-27
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• 2005

The aim of this study was to investigate differentially expressed proteins between normal chicken liver and chicken liver inffeted by Salmonella pullorum. 2-dimensional electrophoresis (2DE) and mass spectrometry (MS) were used to identify the proteins. More than 300 protein spots were detected on silver stained 2DE gels using pH 3$\~$10 gradients. The most outstanding protein spot was further analyzed by MALDI-TOF MS and protein database using the Mascot search engine. The protein was finally identified as APOAI (Apolipoprotein AI). Based on the known function of the APOAI, this gene acts protective action against the accumulation of platelet thrombin at the site of vascular damage for the pullorum disease. Therefore APOAI protein, identified in this study, can be a valuable biomarker in relation to the pullorum disease in chicken.

• Molecules and Cells
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• v.38 no.1
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• pp.89-94
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• 2015

The shape and activity of mitochondria are tightly regulated by fusion and fission processes that are essential for maintaining normal cellular function. However, little is known about the involvement of mitochondrial dynamics in the development of the immune system. In this study, we demonstrate that mitochondrial dynamics play a role in the differentiation and migration of immature dendritic cells (imDCs). We show that mitochondrial elongation is induced during GM-CSF-stimulated differentiation of bone marrow progenitors to imDCs accompanied by upregulation of mitochondrial fusion proteins. These processes precede the changes in mitochondrial morphology and connectivity that occur during differentiation. Mfn2 and OPA1, but not Mfn1, are transcriptionally upregulated during differentiation; however, knockdown of Mfn2 and OPA1 does not induce any change in expression of CD11c, CDC80, or CD86. Notably, knockdown of Mfn2 or OPA1 by siRNA in imDCs significantly reduces CCR7 expression and CCL19-mediated migration. These results suggest that the mitochondrial fusion-related proteins Mfn2 and OPA1 are upregulated during bone marrow progenitor differentiation and promote the migration of imDCs by regulating the expression of CCR7.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.14 no.5
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• pp.413-420
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• 2021

This study is about a machine learning model for predicting insufficient parameters through other parameters when one of the collected parameters is insufficient. A regression model was created to predict time, temperature, humidity, CO2, and light quantity data through the machine learning regression analysis function in Matlab. In addition, the three models with the lowest RMSE values for each parameter were selected and verified. For verification, the predicted values were obtained by applying the test data to the prediction model derived from each parameter, and the correlation coefficient and error average between the measured values and the obtained predicted values were obtained and then compared.

• Molecules and Cells
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• v.42 no.1
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• pp.56-66
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• 2019

Histidine triad nucleotide-binding protein (HINT) is a member of the histidine triad (HIT) superfamily, which has hydrolase activity owing to a histidine triad motif. The HIT superfamily can be divided to five classes with functions in galactose metabolism, DNA repair, and tumor suppression. HINTs are highly conserved from archaea to humans and function as tumor suppressors, translation regulators, and neuropathy inhibitors. Although the structures of HINT proteins from various species have been reported, limited structural information is available for fungal species. Here, to elucidate the structural features and functional diversity of HINTs, we determined the crystal structure of HINT from the pathogenic fungus Candida albicans (CaHINT) in complex with zinc ions at a resolution of $2.5{\AA}$. Based on structural comparisons, the monomer of CaHINT overlaid best with HINT protein from the protozoal species Leishmania major. Additionally, structural comparisons with human HINT revealed an additional helix at the C-terminus of CaHINT. Interestingly, the extended C-terminal helix interacted with the N-terminal loop (${\alpha}1-{\beta}1$) and with the ${\alpha}3$ helix, which appeared to stabilize the dimerization of CaHINT. In the C-terminal region, structural and sequence comparisons showed strong relationships among 19 diverse species from archea to humans, suggesting early separation in the course of evolution. Further studies are required to address the functional significance of variations in the C-terminal region. This structural analysis of CaHINT provided important insights into the molecular aspects of evolution within the HIT superfamily.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.276-284
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• 2022

Endocrine-disrupting chemicals found in many commercial products may interfere with the normal functioning of the endocrine system and are unsafe because of their cumulative effect on the human body. However, little is known about the effects of combinations of endocrine-disrupting chemicals in humans. Methoxychlor and bisphenol A are toxic to male reproductive organs. Therefore, we studied the effects of methoxychlor and bisphenol A on male reproductive function. Male mice were divided into four treatment groups: control, 400 mg methoxychlor, 1 mg bisphenol A, and 400 mg methoxychlor + 1 mg bisphenol A/kg/day. Methoxychlor and bisphenol A were dissolved in sesame oil and acetone and administered orally for 4 weeks. After administration, the weight and histological changes in the testicles and epididymis, sperm count and health were observed biochemical tests and whole blood counts were performed. The results showed that the mice in the bisphenol A and methoxychlor + bisphenol A groups gained more weight than those in the control and methoxychlor group. The weights of the testes and epididymis were higher in the experimental groups than in the control. Sperm motility and progression were significantly reduced in the bisphenol A and methoxychlor + bisphenol A groups. Histological observation showed a reduced number of sperm, smaller seminiferous tubules, and destroyed lumen in the methoxychlor + bisphenol A group compared to the other groups. In conclusion, our study showed that methoxychlor and bisphenol A destroy male reproductive tissues and decrease sperm quality.

• Clinical Nutrition Research
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• v.12 no.4
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• pp.269-282
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• 2023

Vitamin D participates in the biological function of the innate and adaptive immune system and inflammation. We aim to specify the effectiveness of the vitamin D supplementation on the side effects BioNTech, Pfizer vaccination, and immunoglobulin G response against severe acute respiratory syndrome coronavirus 2 in subjects tested positive for coronavirus disease 2019 (COVID-19). In this multi-center randomized clinical trial, 498 people tested positive for COVID-19 were divided into 2 groups, receiving vitamin D capsules or a placebo (1 capsule daily, each containing 600 IU of vitamin D) over 14-16 weeks. Anthropometric indices and biochemical parameters were measured before and after the second dose of vaccination. Fourteen to 16 weeks after supplementation, the intervention group had an immunoglobulin G (IgG) increase of 10.89 ± 1.2 g/L, while the control group had 8.89 ± 1.3 g/L, and the difference was significant between both groups (p = 0.001). After the second dose of vaccination, the supplement group significantly increased their 25-hydroxy vitamin D from initially 28.73 ± 15.6 ng/mL and increased to 46.48 ± 27.2 ng/mL, and the difference between them was significant. Those with a higher body mass index (BMI) had the most of symptoms, and the difference of side effects according to BMI level was significantly different. In 8 weeks after supplementation obese participants had the lowest IgG levels than overweight or normal subjects. The proportion of all types of side effects on the second dose was significantly diminished compared with the first dose in the intervention group. Supplementation of 600 IU of vitamin D3 can reduce post-vaccination side effects and increase IgG levels in participants who received BioNTech, Pfizer vaccine.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.7
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• pp.4416-4422
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• 2014

In the present study, mycosporine-like amino acids (MAAs)were extracted from in Chlamydomonas hedleyi, and their function was investigated regarding to protective capacity against UV radiation and a possibility to be developed into functional suncream including MAAs by using natural compounds. In particulr, we assessed UV protective ability and anti-inflammation of shinorine in human skin cells. As a result, shinorine can protect the skin against damage by the absorption of energy from UV radiation and functions as an anti-wrinkling substrate through a increase of collagen synthesis. These data suggest that shinorine can be utilized not only as a substrate to protect UV radiation, but anti-aging material in cosmetic products.

• Journal of the Korean Wood Science and Technology
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• v.44 no.3
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• pp.350-359
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• 2016

We performed fast pyrolysis of empty fruit bunch (EFB) in the range of temperature from $400{\sim}550^{\circ}C$ and 1.3 s of residence time. The effect of temperature on the yields and physicochemical properties of pyrolytic products were also studied. Elemental and component analysis of EFB showed that the large amount of potassium (ca. 8400 ppm) presents in the feedstock. Thermogravimetric analysis suggested that the potassium in the feedstock catalyzed degradation of cellulose. The yield of bio-oil increased with increasing temperature in the range of temperature from $400{\sim}500^{\circ}C$, while that of gas and biochar decreased and showed monotonous change each with increasing temperature. When the EFB was pyrolyzed at $550^{\circ}C$, the yield of bio-oil and char decreased while that of gas increased. Water content of the bio-oils obtained at different temperatures was 20~30% and their total acid number were less than 100 mg KOH/g oil. Viscosity of the bio-oils was 11 cSt (centistoke), and heating value varied from 15 to 17 MJ/kg. Using GC/MS analysis, 27 chemical compounds which were classified into two groups (cellulose-derived and lignin-derived) were identified. Remarkably the concentration of phenol was approximately 25% based on entire chemical compounds.