• Asian-Australasian Journal of Animal Sciences
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• 제17권12호
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• pp.1641-1646
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• 2004

The objective of this study was to generate transgenic mice expressing human resistin gene by using the tetraploidembryonic stem (ES) cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR, cloned into $pCR^{(R)}$ 2.1 $TOPO^{(R)}$ vector and constructed in pCMV-Tag4C vector. Mammalian expression plasmid containing human resistin was transfected into D3-GL ES cells by Lipofectamine 2,000, and then after 10-12 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec (fusion rate: 2,114/2,256, 93.5%) and cultured up to the blastocyst stage (development rate: 1,862/2,114, 94.6%). The selected 15-20 ES cells were injected into tetraploid blastocysts, and then transferred into the uteri of E 2.5 d pseudopregnant recipient mice. To investigate the gestation progress, two E 19.5 mused fetuses were recovered by Cesarean section of which one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, our findings demonstrate that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mice for the rapid analysis of gene function in vivo.

• 한국수산과학회지
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• 제53권1호
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• pp.67-73
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• 2020

The maturity and spawning of the yellow goosefish Lophius litulon were studied using samples collected monthly from January to December 2018 from the coastal waters of Korea. We analyzed monthly changes in maturity stage, gonadosomatic index (GSI), total length (TL) at 50%, 75%, and 97.5% group maturity, egg diameter and fecundity. The spawning period was from January to June. The size of eggs spawned was between 0.30 to 1.28 mm. Fecundity varied between 328,314 to 1,624,783 eggs. The percentage of sexually mature females estimated from a logistic function was over 50% at 48.67 cm TL, 75% at 56.76 cm TL and 97.5% at 74.89 cm TL.

• 한국수산과학회지
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• 제53권1호
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• pp.43-49
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• 2020

The maturation and spawning of red seabream Pagrus major were investigated using 1,014 samples collected monthly from January to December of 2018, in the South Sea of Korea. Based on monthly changes in maturity stage and gonadosomatic index, the spawning period was estimated to be between April and August, with peak spawning occuring from May to June. Fecundity varied between 228,996 and 4,544,948 eggs. The relationship between fecundity (F) and fork length (FL) in this species can be expressed by the equation F=0.4869FL^3.9452 (R²=0.7448). Using a logistic function, the percentage of sexually mature females was estimated to be over 50% for fish with a FL of 35.3 cm.

• Journal of the Korean Wood Science and Technology
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• 제34권1호
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• pp.32-39
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• 2006

본 연구는 현악기에 사용되는 현악기용 도료가 현악기의 음향성능에 미치는 영향을 조사하였다. 음향분석장치(FFT)를 이용하여 목재악기용 도료의 도막두께에 따른 음향적 성능을 평가하였다. 목재악기용으로 사용된 도료는 악기용 우레탄계 상도도료, 오일 스테인계 도료, 자외선 경화형 에폭시 도료이다. 형성된 도막의 음향적인 특성은 FFT 분석기에서 측정된 공명주파수와 댐핑값(damping factor)으로부터 동적탄성계수와 전단탄성계수를 계산하여 얻고, 밀도와의 관계와 탄성계수간의 관계를 통하여 도막의 두께에 따른 음향적인 특성을 측정하고 비교분석하여 상관관계를 조사하였다.

• 대한수의학회지
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• 제45권2호
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• pp.155-161
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• 2005

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in chickens fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n=60/group) of day old broiler chickens were administered with 0 (control) and 0.8% (treatment) TCM-KWG through drinking water. After 5 weeks, we examined the protein expression pattern of fibularis longus and superficial pectoral muscle by Two-dimensional electrophoresis analysis. Interestingly, TCM-KWG treatment significantly increased five spot's density, and markedly reduced five spot's density in the muscles. We identified 10 proteins (desmin, myosin light chain 1, heat shock 25 kDa protein, collapsin response mediator protein-2A, alpha enolase, vimentin, actin alpha 1, my023 protein, pyruvate kinase and troponin T) by the matrix-assisted laser desorption ionization time of flight (MALDI-TOF).

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1863-1870
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• 2016

Antimicrobial peptides/proteins (AMPs) are present in all types of organisms, from microbes and plants to vertebrates and invertebrates such as insects. The grasshopper Oxya chinensis sinuosa is an insect species that is widely consumed around the world for its broad medicinal value. However, the lack of available genetic information for this species is an obstacle to understanding the full potential of its AMPs. Analysis of the O. chinensis sinuosa transcriptome and expression profile is essential for extending the available genetic information resources. In this study, we determined the whole-body transcriptome of O. chinensis sinuosa and analyzed the potential AMPs induced by bacterial immunization. A high-throughput RNA-Seq approach generated 94,348 contigs and 66,555 unigenes. Of these unigenes, 36,032 (54.14%) matched known proteins in the NCBI database in a BLAST search. Functional analysis demonstrated that 38,219 unigenes were clustered into 5,499 gene ontology terms. In addition, 26 cDNAs encoding novel AMPs were identified by an in silico approach using public databases. Our transcriptome dataset and AMP profile greatly improve our understanding of O. chinensis sinuosa genetics and provide a huge number of gene sequences for further study, including genes of known importance and genes of unknown function.

• 구강회복응용과학지
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• 제31권3호
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• pp.231-241
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• 2015

환자의 심미적 요구가 증가함에 따라 전치뿐만 아니라 구치부에서도 자연치아와 유사한 색조와 외형을 재현한 보철물에 대한 필요성이 증가하고 있다. 단일 지르코니아와 CAD/CAM 시스템을 통해 이러한 환자의 요구를 만족시키는 보철물 제작이 가능해졌다. 본 증례는 70세 여자환자로 전치부 보철물이 깨져서 보기 싫고 하악 구치부가 없어서 저작이 불편하다는 주소로 내원하였다. 하악 구치의 상실로 교합평면이 붕괴되었으며 과도한 치아 마모 및 수직고경 상실이 관찰되어 치아 상실 부위에 임플란트를 식립하고 수직고경 증가와 함께 지르코니아 고정성 보철물을 이용한 전악 수복 치료를 진행하였다. 치료 후 3년 간 이상적인 교합이 잘 유지되고 기능적, 심미적으로 만족한 결과를 얻었기에 이를 보고하는 바이다.

• 구강회복응용과학지
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• 제32권3호
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• pp.240-245
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• 2016

치은 연하 변연을 갖는 임플란트 수복물 장착시 잔여 합착제의 제거는 임플란트 생물학적 합병증을 예방하기 위해 매우 중요하다. 치은 연하 잔여 합착제를 최소화하기 위해 인상용 실리콘이나 자가중합형 레진을 이용하여 복제 지대주를 제작하는 여러 방법이 소개되었다. 본 증례에서는 열가소성 접착제(hot melt adhesive material)를 이용하여 복제 임플란트 지대주(abutment replica)제작하는 방법을 소개하고자 한다. 이 방법은 간단하고 부가적인 기공과정이 필요 없어 진료실에서 쉽게 이용할 수 있어 이에 보고하는 바이다.

• 한국해양바이오학회지
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• 제12권2호
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• pp.81-90
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• 2020

Sialic acid, which is contained in about 60-160 mg/kg in the edible bird's nest (EBN), is known to facilitate in the proper formation of synapses and improve memory function. The objective of this study is to extract effectively the sialic acid from edible bird's nest using affinity bead technology (ABT). After preparing the non-polar polymeric bead "KJM-278-28A" having a porous network structure, and then desorbed sialic acid was concentrated and dried. The analysis of the physicochemical properties of bead "KJM-278-28A" showed that the particle size was 400-700 ㎛, the moisture holding capacity was 67-70%, the surface area (BET) was 705-900 ㎡/g, and the average pore diameter 70-87 Å. The adsorption capacity of the bead "KJM-278-28A" for sialic acid was shown a strong physical force to bind sialic acid to the bead surface of 400 mg/L. In addition, as a result of analyzing the adsorption and desorption effects of sialic acid on water, ethanol, and 10% ethanol on the bead, it was confirmed that desorption effectively occurs from the beads when only ethanol is used. As a result of HPLC measurement of the separated sialic acid solution, a total of four sialic acid peaks of N-acetyl-neuraminic acid (Neu5Ac), α,β-anomer of Neu5Ac and N-glycoly-neuraminic acid were identified. Through these results, it was confirmed that it is possible to separate sialic acid from EBN extract with efficient and high yield when using ABT.

• 반도체디스플레이기술학회지
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• 제20권4호
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• pp.89-95
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• 2021

As the influence of fine dust on society spreads gradually, the public's interest in indoor air is increasingly rising. Air-purifying plants are drawing keen attention due to their natural purifying function enabled by plant physiology. However, as their fine dust reduction mechanism is limited to adsorption only, vegetation bio-filters that optimize purification effects through integration with air-conditioning systems is rising as an alternative. In accordance with the relevant standard test methods, this study looked into the fine dust reduction assessment method by air-conditioning airflow volume that can be used for the industrial spread of vegetation bio-filters. In the case of PM₁₀ at 300 ㎍/m³, it was in the order of EG-B(3,500CMH, 29 min.) < EG-A (2,500CMH, 37 min.) < CG(0CMH, 64 min.) for reaching the maintenance level (100 ㎍/m³) of publicly used facilities. For reaching the WHO Guideline(50 ㎍/m³) requirement, it was in the order of EG-B (51 min.) < EG-A (160 min.) < CG (170 min.). In the case of PM_2.5, it was in the order of EG-B (26 min.) < EG-A (33 min.) < CG (57 min.) for reaching the maintenance level (50 ㎍/m³) of publicly used facilities. It was in the order of EG-B (48 min) < EG-A (140 min) < CG (158 min) for reaching the WHO Guideline (25 ㎍/m³) requirement. The findings from the analysis showed that fine dust can be reduced most efficiently when the system is operated at 3,500CMH level. The limitation of this study is that due to the absence of a way of assessing the stress of plants in vegetation bio-filters, generating optimal air-conditioning air flow of the relevant system and economics analysis against the existing facility-type air purification system have been clarified, which should be explored further though follow-up studies.