• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.207-212
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• 2016

PacBio's long-read sequencing technologies can be successfully used for a complete bacterial genome assembly using recently developed non-hybrid assemblers in the absence of second-generation, high-quality short reads. However, standardized procedures that take into account multiple pre-existing second-generation sequencing platforms are scarce. In addition to Illumina HiSeq and Ion Torrent PGM-based genome sequencing results derived from previous studies, we generated further sequencing data, including from the PacBio RS II platform, and applied various bioinformatics tools to obtain complete genome assemblies for five bacterial strains. Our approach revealed that the hierarchical genome assembly process (HGAP) non-hybrid assembler resulted in nearly complete assemblies at a moderate coverage of ~75x, but that different versions produced non-compatible results requiring post processing. The other two platforms further improved the PacBio assembly through scaffolding and a final error correction.

• Transactions of the Korean hydrogen and new energy society
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• v.28 no.4
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• pp.426-432
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• 2017

Hydrothermal carbonization (HTC) is an effective and environment friendly technique; it possesses extensive potential towards producing high-energy density solid fuels. it is a carbonization method of thermochemical process at a relatively low temperature ($180-250^{\circ}C$). It is reacted by water containing raw material. However, the production and quality of solid fuels from HTC depends upon several parameters; temperature, residence time, and pressure. This study investigates the influence of operating parameters on solid fuel production during HTC. Especially, when food waste was reacted for 2 hours, 4 hours, and 8 hours at $200^{\circ}C$ and 2.0-2.5 MPa, Data including heating value, proximate analysis and water content was consequently collected and analyzed. It was found that reaction temperature, residence time are the primary factors that influence the HTC process.

• Journal of Biosystems Engineering
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• v.26 no.6
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• pp.535-544
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• 2001

This study developed a manipulator for robotic harvester to harvest cucumber. The manipulator was designed and built fur transferring an end-effecter from a fixed point to a specified cucumber. Its development involved the integration of a manipulating system with a PC compatible, DC motors, geared boxes, timing belts, and a motor controller board. Software, written in Quick basic. combined the functions of motor control with various circumstances. In order to move smoothly and rapidly the manipulator, it's shoulder link and elbow link were minimized by using rotational inertial moment without a motor and a geared box. After 30 replications of exercising the manipulator, it was concluded that the precision values of the X, Y and Z axes were less than 0.5mm, 7.25mm and 0.35mm, respectively. The precision data indicated the manipulator was not missing any steps fur the harvester to reach a target cucumber.

• Journal of Biosystems Engineering
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• v.33 no.5
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• pp.296-302
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• 2008

The effective moisture diffusivity and its dependence on drying temperature during drying of rapeseed were experimentally investigated. The data were recorded from thin layer drying experiments at nine different combinations of drying air temperatures of 40, 50, and $60^{\circ}C$ and the relative humidities of 30, 45, and 60%. The moisture diffusion equation was analyzed using stepwise multiple regression analysis. Effective moisture diffusivities were calculated based on the moisture diffusion equation for a spherical shape using Fick's second law. The effective diffusivities during the drying of rapeseed were $l.72{\times}10^{-11}$, $2.41{\times}10^{-11}$ and $3.31{\times}10^{-11}\;m^2{\cdot}s^{-1}$ at 40, 50 and $60^{\circ}C$, respectively. The activation energy for moisture diffusion during drying was $28.47\;kJ{\cdot}mol^{-1}$. The dependence of moisture diffusivity on temperature was described by an Arrhenius-type equation. Drying occurred in the falling rate period and the internal moisture diffusion phenomenon is the governing physical mechanism of the moisture movement in the particles.

• Proceedings of the Korea Contents Association Conference
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• 2011.05a
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• pp.543-544
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• 2011

최근 유전자 염기서열분석방법이 새롭게 등장하면서 대량의 생물정보의 데이터가 축적되고 있다. 다양한 생물종의 유전체 정보에 대하여 관련 연구자들에게 유용한 정보를 제공하기 위해서는 분석된 유전체 정보를 빠르게 전달할 필요가 증가하고 있다. 본 연구에서는 분석된 유전체 정보에 대한 데이터베이스를 구축하고 이를 모바일 환경에서 손쉽게 탐색할 수 있는 클라이언트 앱을 개발하였다. 유전체 정보에 대한 데이터베이스는 BioMart를 사용하였다. BioMart의 데이터베이스 스키마를 기반으로 웹서비시스 방법을 통하여 모바일 환경의 기기와 연동할 수 있도록 하였다. 개발된 시스템의 검증을 위해서 BioMart의 유전자내용을 검색 할 수 있는 질의를 통하여 모바일기기에서 인간의 유전체 정보를 네비게이션 할 수 있도록 하였다.

• Journal of Environmental Science International
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• v.25 no.6
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• pp.757-766
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• 2016

MTBE and other gasoline additives contained in gasoline are known to be a refractory substance resistant to biodegradation. As a method of removing these substances, a research of method using native microbes of polluted soil was progressed and among these, bio-degradation possibility under aerobic condition was evaluated. All of the experiments were progressed based on batch experiment of lab scale and analyzed by GC-FID using HS-SPME technique. The result of bio-degradation experiment based on MTBE and other additives(ETBE, TAME) was observed below 1 mg/L, which initial concentration were 100 mg/L for each method. And through production of by-product and CO2, partial mineralization was confirmed. Degradation velocity of each additive was promptly represented in the order of TBA>ETBE>MTBE>TAME. Through this study, bio-degradation possibility of native microbes of oil polluted soil, MTBE and other gasoline additives was confirmed and it was considered that the result could be used for basic experiment data in removing oil pollutants of soil.

• Natural Product Sciences
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• v.18 no.4
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• pp.273-278
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• 2012

Further phytochemical investigation on the whole plant of Thyrsanthera suborbicularis, collected in Cambodia, led to kaempferol (1), vitexin (2), apigenin-7-O-neohesperidoside (3), chrysoeriol-7-O-${\beta}$-D-glucopyranoside (4), isorhamnetin 3-O-rutinoside (5), kaempferol-3-O-[${\alpha}$-L-rhamnopyranosyl-(13)-${\alpha}$-L-rhamnopyranosyl-(16)-${\beta}$-D-galactopyranoside (6), kaempferol-3-O-${\alpha}$-L-rhamnopyranosyl(12)-O-[${\alpha}$-L-rhamnopyranosyl (16)]-${\beta}$-D-glucopyranoside (7), kaempferol-3-O-[6"-O-(E)-p-coumaroyl]-${\beta}$-D-glucopyranoside (8), kaempferol-3-O-[6"-O-(E)-p-coumaroyl]-${\beta}$-D-galactopyranoside (9), and amentoflavone (10). All the structures were confirmed by the interpretation of NMR (1D and 2D) and MS data, and comparison with the published values. Of the isolated compounds 1 - 10, compounds 8 and 10 displayed the inhibitory activity against NO production in LPS-induced Raw 264.7 cells with $IC_{50}$ values, 3.56 and $15.73{\mu}M$, respectively.

• Membrane and Water Treatment
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• v.8 no.1
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• pp.73-88
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• 2017

Adsorption kinetics of aqueous ferric ion ($Fe^{3+}$) onto bio-natural rice grains (BRG) have been studied in a batch system. The influence of contact time (0-180 minutes), the dosage of BRG adsorbent (10, 20, 40, and $60gL^{-1}$), and ambient temperature (27, 37, 47, and $57^{\circ}C$) for the adsorption system have been reported. The equilibrium time achieved after 20 minutes of adsorption contact time. The maximum removal of ferric ion is 99% by using $60gL^{-1}$ of BRG, $T=37^{\circ}C$, and $50mgL^{-1}$ ferric ion solution. Adsorption kinetic and diffusion models, such as pseudo-first order, pseudo-second order, and Weber-Morris intra-particle diffusion model, have been used to describe the adsorption rate and mechanism of the ferric ion onto BRG surface. The sorption data results are fitted by Lagergren pseudo-second order model ($R^2=1.0$). The kinetic parameters, rate constant, and sorption capacities have been calculated. The new information in this study suggests that BRG could adsorb ferric ion from water physiosorption during the first 5 minutes. Afterward, the electrostatic interaction between ferric ion and BGR-surface could take place as a very weak chemisorptions process. Thus, there is no significant change could be noticed in the FTIR spectra after adsorption. I recommend producing BGR as a bio-natural filtering material for removing the ferric ion from water.

• Fisheries and Aquatic Sciences
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• v.16 no.1
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• pp.25-34
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• 2013

Early ontogenic development in Siberian sturgeon Acipenser baerii was documented and the effects of different temperatures on embryonic and prelarval development were examined. Photograph-assisted data on morphogenesis in Siberian sturgeon prolarvae agreed well with published descriptions of their ontogeny and ecological behaviors, although certain aspects of differentiation, such as gill covering and scute development, could be rearing condition-sensitive. The present study provides the first characterization of the transient development of teeth during early larval stages; the pattern was congruent with the transition of prolarvae to exogenous feeding. From examinations of embryonic and prelarval development under different temperature conditions ($12-24^{\circ}C$), developmental speed was inversely related with temperature. Overall, hatchability was higher and hatching events were more synchronized at $20^{\circ}C$ than at lower temperatures. After hatching, similar patterns of temperature-dependency were observed in yolk sac absorption and the evacuation of the pigment plug. Our results suggest that the incubation of Siberian sturgeon embryos and prolarvae at temperatures close to $20^{\circ}C$ would be advantageous in hatcheries, based on reductions in the duration and uniformity of egg and prolarval developmental stages.

• Korean Journal of Pharmacognosy
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• v.36 no.4 s.143
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• pp.332-337
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• 2005

We here demonstrate the evidence of increased anti-tumor and immunostimulating activities of crude extracts (GAL) from Galium. aparine L. In experimental lung metastasis of colon26-M3.1 carcinoma or B16-BL6 melanoma cells, prophylactically intravenous (i.v) administration of GAL significantly inhibited lung metastasis in a dose-dependant manner. In an in vitro cytotoxicity analysis, GAL at the concentration up to $500\;{\mu}g/ml$ did not affect the growth of B16-BL6 melanoma cells. In contrast, GAL showed the enhancement of splenocyte proliferating activity in a dose-dependent manner. Peritoneal macrophages stimulated with GAL produced various cytokines such as $1L-1{\beta},\;TNF-{\alpha},\;IFN-{\gamma}$ and IL-12. These data suggest that GAL has an antitumor activity to inhibit tumor metastasis, and its antitumor effects is associated with activation of nonspecific immnune related cells.